Cited by 429Neuroscience and Neuropharmacology ResearchNeuropeptides and Animal PhysiologyNeural dynamics and brain function

Immunochemical characterization of inhibitory mouse cortical neurons: Three chemically distinct classes of inhibitory cells

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Xu, Xiangmin, Roby, Keith D., Callaway, Edward M.·2010·Journal of Comparative Neurology

Abstract

The cerebral cortex has diverse types of inhibitory neurons. In rat cortex, past research has shown that parvalbumin (PV), somatostatin (SOM), calretinin (CR), and cholecystokinin (CCK) label four distinct chemical classes of GABAergic interneurons. However, in contrast to rat cortex, previous studies indicate that there is significant colocalization of SOM and CR in mouse cortical inhibitory neurons. In the present study we further characterized immunochemical distinctions among mouse inhibitory cortical neurons by double immunochemical labeling with chemical markers. We found that, PV, SOM, and vasointenstinal peptide (VIP) reliably identify three nonoverlapping distinct subpopulations, as there was no overlap of immunoreactivity between PV and all the other chemical markers tested, and SOM and VIP did not show any overlap in labeled neurons in all the cortical areas. In comparison, there was significant overlap in combinations of other chemical markers. With some laminar and regional variations, the average overlap of SOM/CR (percentage of SOM+ cells expressing CR) and SOM/neuropeptide tyrosine (NPY) across all examined layers and cortical regions was 21.6% and 7.1%, respectively. The average overlap of VIP/CR, VIP/NPY, and CR/NPY was 34.2%, 9.5%, and 10%, respectively. We quantified and assessed the percentages of marker-positive GABAergic cells, and showed that the nonoverlapping subpopulations (i.e., PV+, SOM+ and VIP+ cells) accounted for about 60% of the GABAergic cell population. Taken together, our data reveal important chemical distinctions between mouse inhibitory cortical neurons and indicate that PV, SOM, and VIP can differentially label a majority of mouse inhibitory cortical neurons.

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  • O-LMs express PV, albeit at significantly lower levels than AACs/PVBCs/BiCs, while PV and SST are considered mutually exclusive in the neocortex.

    “On the other hand, O-LMs express PV, albeit at significantly lower levels than AACs/PVBCs/BiCs, while PV and SST are considered mutually exclusive in the neocortex (324, 344, 561, 568, 582, 721, 1142, 1237).”

    From Hippocampal GABAergic Inhibitory Interneurons (2017) · cites this paper as [1237]

  • PV+ and SST+ interneurons form relatively non-overlapping interneuron populations.

    “As noted earlier, PV+ and SST+ interneurons form relatively non-overlapping interneuron populations [132, 133].”

    From An emerging circuit pharmacology of GABAA receptors (2018) · cites this paper as [133]

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Research works printed but not linked: 2

  1. Meskenaite V 1997 Calretinin-immunoreactive local circuit neurons in area 17 of the cynomolgus monkey, Macaca fascicularis The Journal of comparative neurology 379 113 132 9057116
  2. Somogyi P Tamas G Lujan R Buhl EH 1998 Salient features of synaptic organisation in the cerebral cortex Brain Res Brain Res Rev 26 113 135 9651498

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Authors
Xu, X., Roby, K. D., & Callaway, E. M.
Affiliations
Salk Institute for Biological Studies (US)
Corresponding author
Callaway, Edward M.
Venue
Journal of Comparative Neurology
Publisher
Wiley
Published
2009-11-30
Volume
518
Issue
3
Pages
389-404
ISSN
  • 0021-9967 (ISSN-L)
  • 1096-9861
OpenAlex
W2066101989
Cited by
429
References
23
Citations by year
2026: 10 · 2025: 25 · 2024: 18 · 2023: 15 · 2022: 30 · 2021: 23 · 2020: 39 · 2019: 29 · 2018: 32 · 2017: 32 · 2016: 32 · 2015: 31 · 2014: 27 · 2013: 29 · 2012: 23
FWCI
7.77
Type
journal article
Language
en
Open access
Locations
  • The Journal of Comparative Neurology (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • National Institute of Mental Health (MH063912, R01MH063912)
  • National Institute on Drug Abuse (R00DA023700, K99DA023700, DA023700)
  • National Eye Institute (EY-010742, R01EY010742)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
  • Life Sciences › Neuroscience › Cognitive Neuroscience › Neural dynamics and brain function
Keywords
GABAergic interneurons, parvalbumin, somatostatin, vasoactive intestinal peptide, neuropeptide tyrosine, calretinin, cholecystokinin, neuropeptide co-localization, mouse cortex, interneuron subpopulations
MeSH terms
Animals, Brain Chemistry, Cerebral Cortex, gamma-Aminobutyric Acid, Glutamate Decarboxylase, Immunohistochemistry, Interneurons, Mice, Inbred C57BL, Mice, Transgenic, Neural Inhibition, Synaptic Transmission, Neuropeptide Y, Neuropeptides, Parvalbumins, Phenotype, Recombinant Fusion Proteins, Somatostatin, Vasoactive Intestinal Peptide, Biomarkers, Green Fluorescent Proteins, Mice, S100 Calcium Binding Protein G, Calbindin 2

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