Diagnosis of Neuroinvasive Astrovirus Infection in an Immunocompromised Adult With Encephalitis by Unbiased Next-Generation Sequencing
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Metagenomic next-generation sequencing (NGS) was used to diagnose an unusual and fatal case of progressive encephalitis in an immunocompromised adult presenting at disease onset as bilateral hearing loss. The sequencing and confirmatory studies revealed neuroinvasive infection of the brain by an astrovirus belonging to a recently discovered VA/HMO clade.
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Human host background limitation may be overcome with brute force due to increasing sequencer capacities; e.g., astrovirus detected by ultradeep sequencing of brain tissue yielded 1,612 reads out of ~134 million (0.0012%).
“For instance, an astrovirus was detected in a child with encephalitis by ultradeep sequencing of brain tissue, yielding only 1,612 reads out of ~134 million (0.0012%) sequences103.”
From Clinical metagenomics (2019) · cites this paper as [103]
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- Authors
- Naccache, S. N., Peggs, K. S., Mattes, F. M., Phadke, R., Garson, J. A., Grant, P., Samayoa, E., Federman, S., Miller, S., Lunn, M. P., Gant, V., & Chiu, C. Y.
- Affiliations
- University of California, San Francisco (US)
- University College London (GB)
- University College London Hospitals NHS Foundation Trust (GB)
- Corresponding author
- Naccache, Samia N.
- ORCID
- Venue
- Clinical Infectious Diseases
- Publisher
- Oxford University Press (OUP)
- Published
- 2015-01-07
- Volume
- 60
- Issue
- 6
- Pages
- 919-923
- ISSN
- 1058-4838 (ISSN-L)
- 1537-6591
- PMC
- PMC4345816
- OpenAlex
- W2164367095
- Cited by
- 294
- References
- 13
- Citations by year
- 2026: 5 · 2025: 15 · 2024: 21 · 2023: 17 · 2022: 22 · 2021: 23 · 2020: 23 · 2019: 38 · 2018: 35 · 2017: 37 · 2016: 44 · 2015: 14
- FWCI
- 33.74
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- cc-by-nc-nd
- Locations
- Clinical Infectious Diseases (publishedVersion, cc-by-nc-nd)
- Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-nd)
- PubMed Central (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- UCL Discovery (University College London) (submittedVersion)
- eScholarship (California Digital Library) (submittedVersion)
- HighWire Press Open Archive (submittedVersion)
- Funders
- NIHR Biomedical Research Centre, Royal Marsden NHS Foundation Trust/Institute of Cancer Research
- National Institute for Health and Care Research
- University College London
- National Institutes of Health
- University of California, San Francisco
- Medical Research Council (MR/K000608/1)
- National Heart, Lung, and Blood Institute (R01HL105704)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Infectious Encephalopathies and Encephalitis
- Health Sciences › Medicine › Infectious Diseases › Viral gastroenteritis research and epidemiology
- Health Sciences › Medicine › Epidemiology › Virology and Viral Diseases
- Keywords
- immunocompromised adults, bilateral hearing loss, metagenomic next-generation sequencing, viral diagnosis, fatal infection
- MeSH terms
- Adult, Mamastrovirus, Brain, Hearing Loss, Bilateral, Humans, Leukemia, Lymphoid, Phylogeny, Fatal Outcome, Encephalitis, Viral, Astroviridae Infections, Capsid Proteins, High-Throughput Nucleotide Sequencing
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