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Cited by 234Genomics and Chromatin DynamicsChromatin Remodeling and CancerNuclear Structure and Function

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The chromatin remodeling complex NoRC targets HDAC1 to the ribosomal gene promoter and represses RNA polymerase I transcription

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Zhou, Yonggang, Santoro, Raffaella, Grummt, Ingrid·2002·The EMBO Journal
DOI 10.1093/emboj/cdf460PMC PMC126197
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Abstract

Mammalian chromatin remodeling complexes are involved in both activation and repression of transcription. Here, we show that NoRC, a SNF2h- containing nucleolar chromatin remodeling complex, represses ribosomal gene transcription. NoRC-mediated rDNA silencing was alleviated by trichostatin A, indicating that histone deacetylation is causally involved in silencing. Chromatin immunoprecipitation experiments demonstrate that overexpression of TIP5, the large subunit of NoRC, mediates deacetylation of nucleosomes in the vicinity of the rDNA promoter. Protein-protein interaction assays reveal association of TIP5 with the histone deacetylase HDAC1 in vivo and in vitro. Deletion of the C-terminal PHD finger and bromodomain abolishes the interaction of TIP5 and HDAC1, and abrogates transcriptional repression. The results suggest that NoRC silences the rDNA locus by targeting the SIN3 corepressor complex to the rDNA promoter, thereby establishing a repressed chromatin structure.

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Metadata

Authors
Zhou, Y., Santoro, R., & Grummt, I.
Affiliations
German Cancer Research Center (DE)
Corresponding author
Grummt, Ingrid
ORCID
Venue
The EMBO Journal
Publisher
Springer Science and Business Media LLC
Published
2002-09-02
Volume
21
Issue
17
Pages
4632-4640
ISSN
  • 0261-4189 (ISSN-L)
  • 1460-2075
DOI
10.1093/emboj/cdf460
PMC
PMC126197
OpenAlex
W2045365624
Cited by
234
References
0
Citations by year
2026: 2 · 2025: 2 · 2024: 6 · 2023: 4 · 2022: 9 · 2021: 5 · 2020: 8 · 2019: 6 · 2018: 11 · 2017: 6 · 2016: 12 · 2015: 9 · 2014: 10 · 2013: 12 · 2012: 15
FWCI
4.99
Type
journal article
Language
en
Open access
Licence
Locations
  • The EMBO Journal (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Deutsches Krebsforschungszentrum
  • Deutsche Forschungsgemeinschaft
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Chromatin Remodeling and Cancer
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
Keywords
norC, HDAC1, histone deacetylation, ribosomal DNA transcription, rDNA silencing, RNA polymerase I, PHD finger, bromodomain, transcriptional repression
MeSH terms
Bromodomain Containing Proteins, Acetylation, Animals, Cell Line, Chromatin, Chromosomal Proteins, Non-Histone, DNA, Ribosomal, Histone Deacetylases, Histones, Multienzyme Complexes, Nucleosomes, Promoter Regions, Genetic, Protein Processing, Post-Translational, Recombinant Fusion Proteins, RNA Polymerase I, Transcription, Genetic, Trans-Activators, 3T3 Cells, Protein Structure, Tertiary, Genes, rRNA, Amino Acid Motifs, Gene Silencing, Protein Interaction Mapping, Macromolecular Substances, Mice, Sin3 Histone Deacetylase and Corepressor Complex, Histone Deacetylase 1

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