Cited by 226Porphyrin Metabolism and DisordersIron Metabolism and DisordersMetalloenzymes and iron-sulfur proteins

Iron use for haeme synthesis is under control of the yeast frataxin homologue (Yfh1)

Lesuisse, E.·2003·Human Molecular Genetics

Abstract

The YFH1 gene is the yeast homologue of the human FRDA gene, which encodes the frataxin protein. Saccharomyces cerevisiae cells lacking the YFH1 gene showed very low cytochrome content. In Deltayfh1 strains, the level of ferrochelatase (Hem15p) was very low, as a result of transcriptional repression of HEM15. However, the low amount of Hem15p was not the cause of haeme deficiency in Deltayfh1 cells. Ferrochelatase, a mitochondrial protein, able to mediate insertion of iron or zinc into the porphyrin precursor, made primarily the zinc protoporphyrin product. Zinc protoporphyrin instead of haeme accumulated during growth of Deltayfh1 mutant cells and, furthermore, preferential formation of zinc protoporphyrin was observed in real time. The method for these studies involved direct presentation of porphyrin to mitochondria and to ferrochelatase of permeabilized cells with intact architecture, thereby specifically testing the iron delivery portion of the haeme biosynthetic pathway. The studies showed that Deltayfh1 mutant cells are defective in iron use by ferrochelatase. Mössbauer spectroscopic analysis showed that iron was present as amorphous nano-particles of ferric phosphate in Deltayfh1 mitochondria, which could explain the unavailability of iron for haeme synthesis. A high frequency of suppressor mutations was observed, and the phenotype of such mutants was characterized by restoration of haeme synthesis in the absence of Yfh1p. Suppressor strains showed a normal cytochrome content, normal respiration, but remained defective in Fe-S proteins and still accumulated iron into mitochondria although to a lesser extent. Yfh1p and Hem15p were shown to interact in vitro by Biacore studies. Our results suggest that Yfh1 mediates iron use by ferrochelatase.

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Crossref · 10.1093/hmg/ddg096

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Authors
Lesuisse, E.
Affiliations
Centre National de la Recherche Scientifique (FR)
Corresponding author
Lesuisse, E.
Venue
Human Molecular Genetics
Published
2003-04-15
Volume
12
Issue
8
Pages
879-889
ISSN
  • 0964-6906 (ISSN-L)
  • 1460-2083
OpenAlex
W2163221921
Cited by
226
References
48
Citations by year
2026: 1 · 2025: 2 · 2024: 3 · 2023: 4 · 2022: 3 · 2021: 5 · 2020: 5 · 2019: 9 · 2018: 11 · 2017: 6 · 2016: 5 · 2015: 9 · 2014: 7 · 2013: 13 · 2012: 9
FWCI
6.32
Type
journal article
Language
en
Open access
closed
Locations
  • Human Molecular Genetics (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Institute of Diabetes and Digestive and Kidney Diseases (DK 53953)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Porphyrin Metabolism and Disorders
  • Health Sciences › Medicine › Hematology › Iron Metabolism and Disorders
  • Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Metalloenzymes and iron-sulfur proteins
Keywords
Yfh1, frataxin, ferrochelatase, Saccharomyces cerevisiae, iron delivery, zinc protoporphyrin, cytochrome biosynthesis, mitochondrial iron, iron-sulfur proteins, suppressor mutations, protein-protein interaction, frdA
MeSH terms
Frataxin, Cytochromes, Ferrochelatase, Fungal Proteins, Genes, Fungal, Heme, Iron, Mitochondria, Protoporphyrins, Saccharomyces cerevisiae, Suppression, Genetic, Gene Deletion, Iron-Binding Proteins

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