Urinary vitamin D-binding protein is elevated in patients with endometriosis
Abstract
BACKGROUND: Recently, proteomic technologies have demonstrated that several proteins are differently expressed in various body fluids of patients with endometriosis compared with those without this condition. The aim of this study was to investigate proteins secreted in urine of patients with endometriosis using proteomic techniques in order to identify potential markers for the clinical diagnosis of endometriosis. METHODS: Urine samples were collected from women undergoing laparoscopy for different indications including pelvic masses, pelvic pain, suspicious endometriosis, infertility and diagnostic evaluation. Proteomic techniques and mass spectrometry were used to identify proteins secreted in the urine of the patients with and without endometriosis and quantification of identified protein was performed using western blot and specific commercial sandwich enzyme-linked immunosorbent assays (ELISA). RESULTS: Twenty-two protein spots were differentially expressed in the urine of patients with and without endometriosis, one of which was identified as urinary vitamin D-binding protein (VDBP). ELISA quantification of urinary VDBP corrected for creatinine expression (VDBP-Cr) revealed that urinary VDBP-Cr was significantly greater in patients with endometriosis than in those without (111.96 ± 74.59 versus 69.90 ± 43.76 ng/mg Cr, P = 0.001). VDBP-Cr had limited value as a diagnostic marker for endometriosis (Sensitivity 58%, Specificity 76%). When combined with serum CA-125 levels (the product of serum CA-125 and urinary VDBP-Cr), it did not significantly increase the diagnostic power of serum CA-125 alone. CONCLUSIONS: Urinary VDBP levels are elevated in patients with endometriosis. They have limited value as a potential diagnostic biomarker for endometriosis but suggest it would be worthwhile to investigate other urinary proteins for this purpose.
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Cho et al. identified significantly higher levels of 22 urine proteins in women with endometriosis including vitamin D-binding protein, prealbumin, enolase-1, and alpha1-antitrypsin using 2D-PAGE and tandem MS.
“Cho et al. used 2D-PAGE and tandem MS to identify significantly higher levels of 22 urine proteins in women with endometriosis including vitamin D-binding protein, prealbumin, enolase-1, and alpha1-antitrypsin [107].”
From Update on biomarkers for the detection of endometriosis (2015) · cites this paper as [107]
As individual analytes, the 22 urine proteins identified by Cho et al. evidenced insufficient sensitivity and specificity for use as a biomarker.
“As individual analytes, these proteins evidenced insufficient sensitivity and specificity for use as a biomarker.”
From Update on biomarkers for the detection of endometriosis (2015) · cites this paper as [107]
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“used 2D-PAGE and tandem MS to identify significantly higher levels of 22 urine proteins in women with endometriosis including vitamin D-binding protein, prealbumin, enolase-1, and alpha1antitrypsin [107].”
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Self-citation
- Authors
- Cho, S., Choi, Y. S., Yim, S. Y., Yang, H. I., Jeon, Y. E., Lee, K. E., Kim, H., Seo, S. K., & Lee, B. S.
- Affiliations
- Yonsei University (KR)
- Corresponding author
- Lee, B. S.
- Venue
- Human Reproduction
- Publisher
- Oxford University Press (OUP)
- Published
- 2011-12-08
- Volume
- 27
- Issue
- 2
- Pages
- 515-522
- ISSN
- 0268-1161 (ISSN-L)
- 1460-2350
- OpenAlex
- W2170070360
- Cited by
- 74
- References
- 47
- Citations by year
- 2026: 1 · 2024: 9 · 2023: 4 · 2022: 7 · 2021: 5 · 2020: 6 · 2019: 3 · 2018: 6 · 2017: 5 · 2016: 5 · 2015: 6 · 2014: 3 · 2013: 12 · 2012: 2
- FWCI
- 6.10
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Human Reproduction (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Reproductive Medicine › Endometriosis Research and Treatment
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Ectopic Pregnancy Diagnosis and Management
- Health Sciences › Medicine › Obstetrics and Gynecology › Gynecological conditions and treatments
- Keywords
- endometriosis, urinary biomarkers, diagnostic biomarkers, urinary proteome, CA125, differentially expressed proteins, serum biomarkers
- MeSH terms
- Adult, alpha 1-Antitrypsin, DNA-Binding Proteins, Endometriosis, Female, Humans, Middle Aged, Phosphopyruvate Hydratase, Prealbumin, Sensitivity and Specificity, Biomarkers, Tumor, Vitamin D-Binding Protein, Biomarkers, Up-Regulation, Protein Disulfide-Isomerases, Protein Subunits, Tumor Suppressor Proteins, Young Adult
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