Combined effects of 3-nitrooxypropanol and canola oil supplementation on methane emissions, rumen fermentation and biohydrogenation, and total tract digestibility in beef cattle

Official Methods of Analysis of AOAC International

Cunniff, P. (2019) · doi:10.1093/9780197610138.001.0001 · Cited by 12,213 · see citing works

Official Methods of Analysis of AOAC International

Horwitz, W., & LATIMER, G. W. (2006) · Medical Entomology and Zoology · Cited by 10,948 · see citing works

Anthropogenic and Natural Radiative Forcing

Myhre, G., Shindell, D., & Pongratz, J. (2014) · Cambridge University Press eBooks · doi:10.1017/cbo9781107415324.018 · Cited by 4,634 · see citing works

Nutrient requirements of beef cattle

Board on Agriculture (1963) · Cited by 3,663 · see citing works

Methane emissions from cattle

Johnson, K. A., & Johnson, D. E. (1995) · Journal of Animal Science · doi:10.2527/1995.7382483x · Cited by 2,714 · cited 2× · see citing works

Nutrient Requirements of Beef Cattle

Perry, T. W. (1995) · Elsevier eBooks · doi:10.1016/b978-012552052-2/50026-3 · Cited by 2,121 · see citing works

Rapid method for determination of total fatty acid content and composition of feedstuffs and feces

Sukhija, P. S., & Palmquist, D. L. (1988) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf00084a019 · Cited by 1,736 · see citing works

Nutritional management for enteric methane abatement: a review

Beauchemin, K. A., Kreuzer, M., O’Mara, F. P., & McAllister, T. A. (2008) · Australian Journal of Experimental Agriculture · doi:10.1071/ea07199 · Cited by 1,162 · see citing works

Agriculture, Forestry and Other Land Use (AFOLU)

Smith, P., Bustamante, M., Ahammad, H., Clark, H., Dong, H., Elsiddig, E. A., Haberl, H., Harper, R. J., House, J. I., Jafari, M., et al. (2014) · Murdoch University Research Portal · Cited by 868 · see citing works

Influence of hydrogen on rumen methane formation and fermentation balances through microbial growth kinetics and fermentation thermodynamics

Janssen, P. H. (2010) · Animal Feed Science and Technology · doi:10.1016/j.anifeedsci.2010.07.002 · Cited by 848 · see citing works

Evaluating acid and base catalysts in the methylation of milk and rumen fatty acids with special emphasis on conjugated dienes and total trans fatty acids

Kramer, J. K. G., Fellner, V., Dugan, M. E. R., Sauer, F. D., Mossoba, M. M., & Yurawecz, M. P. (1997) · Lipids · doi:10.1007/s11745-997-0156-3 · Cited by 814 · see citing works

BOARD-INVITED REVIEW: The hepatic oxidation theory of the control of feed intake and its application to ruminants

Allen, M. S., Bradford, B. J., & Oba, M. (2009) · Journal of Animal Science · doi:10.2527/jas.2009-1779 · Cited by 633 · see citing works

Atlas of rumen microbiology.

OGIMOTO, K., & Soichi, I. M. A. I. (1981) · Cited by 626 · see citing works

Review: Fifty years of research on rumen methanogenesis: lessons learned and future challenges for mitigation

Beauchemin, K. A., Ungerfeld, E. M., Eckard, R. J., & Wang, M. (2020) · Animal · doi:10.1017/s1751731119003100 · Cited by 619 · see citing works

Microscale determination of inorganic nitrogen in water and soil extracts

Sims, G. K., Ellsworth, T. R., & Mulvaney, R. L. (1995) · Communications in Soil Science and Plant Analysis · doi:10.1080/00103629509369298 · Cited by 571 · see citing works

Redirecting rumen fermentation to reduce methanogenesis

McAllister, T. A., & Newbold, C. J. (2008) · Australian Journal of Experimental Agriculture · doi:10.1071/ea07218 · Cited by 501 · see citing works

An inhibitor persistently decreased enteric methane emission from dairy cows with no negative effect on milk production

Hristov, A. N., Oh, J., Giallongo, F., Frederick, T. W., Harper, M. T., Weeks, H. L., Branco, A. F., Moate, P. J., Deighton, M. H., Williams, S. R. O., et al. (2015) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1504124112 · Cited by 496 · see citing works

Methane emissions from beef cattle: Effects of monensin, sunflower oil, enzymes, yeast, and fumaric acid1

McGinn, S. M., Beauchemin, K. A., Coates, T., & Colombatto, D. (2004) · Journal of Animal Science · doi:10.2527/2004.82113346x · Cited by 477 · see citing works

Combining Results of Two GC Separations Partly Achieves Determination of All cis and trans 16:1, 18:1, 18:2 and 18:3 Except CLA Isomers of Milk Fat as Demonstrated Using Ag‐Ion SPE Fractionation

Kramer, J. K. G., Hernandez, M., Cruz‐Hernandez, C., Kraft, J., & Dugan, M. E. R. (2008) · Lipids · doi:10.1007/s11745-007-3143-4 · Cited by 381 · see citing works

Methane emissions from beef cattle: Effects of fumaric acid, essential oil, and canola oil1

Beauchemin, K. A., & McGinn, S. M. (2006) · Journal of Animal Science · doi:10.2527/2006.8461489x · Cited by 379 · see citing works

Mode of action uncovered for the specific reduction of methane emissions from ruminants by the small molecule 3-nitrooxypropanol

Duin, E. C., Wagner, T., Shima, S., Prakash, D., Cronin, B., Yáñez-Ruiz, D. R., Duval, S., Rümbeli, R., Stemmler, R. T., Thauer, R. K., et al. (2016) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1600298113 · Cited by 339 · see citing works

The effect of dietary fats on methane emissions, and its other effects on digestibility, rumen fermentation and lactation performance in cattle: A meta-analysis

Patra, A. K. (2013) · Livestock Science · doi:10.1016/j.livsci.2013.05.023 · Cited by 287 · see citing works

Epithelial Capacity for Apical Uptake of Short Chain Fatty Acids Is a Key Determinant for Intraruminal pH and the Susceptibility to Subacute Ruminal Acidosis in Sheep ,

Penner Gregory, B., Aschenbach, J. R., Gäbel, G., Rackwitz, R., & Oba, M. (2009) · Journal of Nutrition · doi:10.3945/jn.109.108506 · Cited by 207 · see citing works

Propionate precursors and other metabolic intermediates as possible alternative electron acceptors to methanogenesis in ruminal fermentation in vitro

Newbold, C. J., López, S., Nelson, N., Ouda, J. O., Wallace, R. J., & Moss, A. R. (2005) · British Journal Of Nutrition · doi:10.1079/bjn20051445 · Cited by 202 · see citing works

Effect of high-oil corn or added corn oil on ruminal biohydrogenation of fatty acids and conjugated linoleic acid formation in beef steers fed finishing diets

Duckett, S. K., Andrae, J. G., & Owens, F. N. (2002) · Journal of Animal Science · doi:10.2527/2002.80123353x · Cited by 177 · see citing works

Rumen Microbes, Enzymes and Feed Digestion-A Review

Wang, Y., & McAllister, T. A. (2002) · Asian-Australasian Journal of Animal Sciences · doi:10.5713/ajas.2002.1659 · Cited by 171 · see citing works

Invited review: Role of rumen biohydrogenation intermediates and rumen microbes in diet-induced milk fat depression: An update

Dewanckele, L., Toral, P. G., Vlaeminck, B., & Fievez, V. (2020) · Journal of Dairy Science · doi:10.3168/jds.2019-17662 · Cited by 161 · see citing works

Determination of Dietary Starch in Animal Feeds and Pet Food by an Enzymatic-Colorimetric Method: Collaborative Study

Hall, M. B., Collaborators:, Arbaugh, J., Binkerd, K., Carlson, A., Doan, T., Grant, T., Heuer, C., Inerowicz, H. D., Jean-Louis, B., et al. (2015) · Journal of AOAC International · doi:10.5740/jaoacint.15-012 · Cited by 151 · see citing works

Effects of ethyl-3-nitrooxy propionate and 3-nitrooxypropanol on ruminal fermentation, microbial abundance, and methane emissions in sheep

Martı́nez, G., Abecia, L., Arco, A., Cantalapiedra-Hijar, G., Martín-García, A. I., Molina‐Alcaide, E., Kindermann, M., Duval, S. M., & Yáñez-Ruíz, D. R. (2014) · Journal of Dairy Science · doi:10.3168/jds.2013-7398 · Cited by 140 · see citing works

Additive methane-mitigating effect between linseed oil and nitrate fed to cattle1

Guyader, J., Eugène, M., Meunier, B., Doreau, M., Morgavi, D., Silberberg, M., Rochette, Y., Gérard, C., Loncke, C., & Martin, C. (2015) · Journal of Animal Science · doi:10.2527/jas.2014-8196 · Cited by 135 · see citing works

Effects of 3-nitrooxypropanol on rumen fermentation, lactational performance, and resumption of ovarian cyclicity in dairy cows

Audino Melgar, M., Harper, M. T., Oh, J., Giallongo, F., Young, M.-P., OTT, T. L., Duval, S. M., & Hristov, A. N. (2019) · Journal of Dairy Science · doi:10.3168/jds.2019-17085 · Cited by 131 · see citing works

Sustained reduction in methane production from long-term addition of 3-nitrooxypropanol to a beef cattle diet1

Romero-Pérez, A., Okine, E. K., McGinn, S. M., Guan, L., Oba, M., Duval, S. M., Kindermann, M., & Beauchemin, K. A. (2015) · Journal of Animal Science · doi:10.2527/jas.2014-8726 · Cited by 128 · see citing works

The combined effects of supplementing monensin and 3-nitrooxypropanol on methane emissions, growth rate, and feed conversion efficiency in beef cattle fed high-forage and high-grain diets1

Vyas, D., Alemu, A. W., McGinn, S. M., Duval, S. M., Kindermann, M., & Beauchemin, K. A. (2018) · Journal of Animal Science · doi:10.1093/jas/sky174 · Cited by 120 · see citing works

Effect of camelina oil or live yeasts (Saccharomyces cerevisiae) on ruminal methane production, rumen fermentation, and milk fatty acid composition in lactating cows fed grass silage diets

Bayat, A. R., Kairenius, P., Stefański, T., Leskinen, H., Comtet-Marre, S., Forano, E., Chaucheyras-Durand, F., & Shingfield, K. J. (2015) · Journal of Dairy Science · doi:10.3168/jds.2014-7976 · Cited by 118 · see citing works

The potential of 3-nitrooxypropanol to lower enteric methane emissions from beef cattle1

Romero-Pérez, A., Okine, E. K., McGinn, S. M., Guan, L. L., Oba, M., Duval, S. M., Kindermann, M., & Beauchemin, K. A. (2014) · Journal of Animal Science · doi:10.2527/jas.2014-7573 · Cited by 113 · see citing works

Linseed oil supplementation to dairy cows fed diets based on red clover silage or corn silage: Effects on methane production, rumen fermentation, nutrient digestibility, N balance, and milk production

Benchaar, C., Hassanat, F., Martineau, R., & Gervais, R. (2015) · Journal of Dairy Science · doi:10.3168/jds.2015-9398 · Cited by 112 · see citing works

Dose-response effect of 3-nitrooxypropanol on enteric methane emissions in dairy cows

Melgar, A., Welter, K. C., Nedelkov, K., Martins, C. M. M. R., Harper, M. T., Oh, J., Räisänen, S. E., Chen, X., Cueva, S. F., Duval, S., et al. (2020) · Journal of Dairy Science · doi:10.3168/jds.2019-17840 · Cited by 112 · see citing works

Effects of coconut and fish oils on ruminal methanogenesis, fermentation, and abundance and diversity of microbial populations in vitro

Patra, A. K., & Yu, Z. (2013) · Journal of Dairy Science · doi:10.3168/jds.2012-6159 · Cited by 92 · see citing works

Effects of sustained reduction of enteric methane emissions with dietary supplementation of 3-nitrooxypropanol on growth performance of growing and finishing beef cattle1

Vyas, D., McGinn, S. M., Duval, S. M., Kindermann, M., & Beauchemin, K. A. (2016) · Journal of Animal Science · doi:10.2527/jas.2015-0268 · Cited by 89 · see citing works

The effects of feeding 3-nitrooxypropanol at two doses on milk production, rumen fermentation, plasma metabolites, nutrient digestibility, and methane emissions in lactating Holstein cows

Haisan, J., Sun, Y., Guan, L. L., Beauchemin, K. A., Iwaasa, A., Duval, S. M., Kindermann, M., Barreda, D. R., & Oba, M. (2016) · Animal Production Science · doi:10.1071/an15219 · Cited by 88 · see citing works

Future Earth and Sustainable Developments

Cheng, H. (2020) · The Innovation · doi:10.1016/j.xinn.2020.100055 · Cited by 86 · see citing works

3-Nitrooxypropanol decreases methane emissions and increases hydrogen emissions of early lactation dairy cows, with associated changes in nutrient digestibility and energy metabolism

van Gastelen, S., Dijkstra, J., Binnendijk, G. P., Duval, S. M., Heck, J. M. L., Kindermann, M., Zandstra, T., & Bannink, A. (2020) · Journal of Dairy Science · doi:10.3168/jds.2019-17936 · Cited by 84 · see citing works

The effects of dietary supplementation with 3-nitrooxypropanol on enteric methane emissions, rumen fermentation, and production performance in ruminants: a meta-analysis

Kim, H., Lee, H. G., Baek, Y.-C., Lee, S., & Seo, J. (2020) · Journal of Animal Science and Technology · doi:10.5187/jast.2020.62.1.31 · Cited by 77 · see citing works

Optimal dose of 3-nitrooxypropanol for decreasing enteric methane emissions from beef cattle fed high-forage and high-grain diets

Vyas, D., McGinn, S. M., Duval, S. M., Kindermann, M., & Beauchemin, K. A. (2016) · Animal Production Science · doi:10.1071/an15705 · Cited by 75 · see citing works

Dietary supplementation with tannin and soybean oil on intake, digestibility, feeding behavior, ruminal protozoa and methane emission in sheep

Lima, P. R., Apdini, T., Freire, A. S., de Sant’ana, A. S., Moura, L. M. L., Nascimento, J. C. S., Rodrigues, R. T. D. S., Dijkstra, J., Neto, A. F. G., Queiróz, M. A. Á., et al. (2019) · Animal Feed Science and Technology · doi:10.1016/j.anifeedsci.2019.01.017 · Cited by 65 · see citing works

Simulation of the effects of diet on the contribution of rumen protozoa to degradation of fibre in the rumen

Dijkstra, J., & Tamminga, S. (1995) · British Journal Of Nutrition · doi:10.1079/bjn19950166 · Cited by 64 · see citing works

Dietary forage to concentrate ratio and sunflower oil supplement alter rumen fermentation, ruminal methane emissions, and nutrient utilization in lactating cows1

Bayat, A. R., Ventto, L., Kairenius, P., Stefański, T., Leskinen, H., Tapio, I., Negussie, E., Vilkki, J., & Shingfield, K. J. (2017) · Translational Animal Science · doi:10.2527/tas2017.0032 · Cited by 59 · see citing works

Effects of 3-nitrooxypropanol on enteric methane production, rumen fermentation, and feeding behavior in beef cattle fed a high-forage or high-grain diet1

Kim, S., Lee, C., Pechtl, H. A., Hettick, J. M., Campler, M. R., Pairis-Garcia, M. D., Beauchemin, K. A., Celi, P., & Duval, S. M. (2019) · Journal of Animal Science · doi:10.1093/jas/skz140 · Cited by 55 · see citing works

Feed intake and methane emissions from cattle grazing pasture sprayed with canola oil

Pinares-Patiño, C. S., Franco, F. E., Molano, G., Kjestrup, H., Sandoval, E., MacLean, S., Battistotti, M., Koolaard, J. P., & Laubach, J. (2015) · Livestock Science · doi:10.1016/j.livsci.2015.11.020 · Cited by 35 · see citing works

Molecular hydrogen generated by elemental magnesium supplementation alters rumen fermentation and microbiota in goats

Wang, M., Wang, R., Zhang, X., Ungerfeld, E. M., Long, D., Mao, H., Jiao, J., Beauchemin, K. A., & Tan, Z. (2017) · British Journal Of Nutrition · doi:10.1017/s0007114517002161 · Cited by 34 · see citing works

Corn oil supplementation enhances hydrogen use for biohydrogenation, inhibits methanogenesis, and alters fermentation pathways and the microbial community in the rumen of goats

Zhang, X., Medrano, R. F., Wang, M., Beauchemin, K. A., Ma, Z. Y., Wang, R., Wen, J. N., Lukuyu, B. A., Tan, Z. L., & He, J. (2019) · Journal of Animal Science · doi:10.1093/jas/skz352 · Cited by 32 · see citing works

3-Nitrooxypropanol supplementation had little effect on fiber degradation and microbial colonization of forage particles when evaluated using the in situ ruminal incubation technique

Zhang, X., Gruninger, R. J., Alemu, A. W., Wang, M., Tan, Z. L., Kindermann, M., & Beauchemin, K. A. (2020) · Journal of Dairy Science · doi:10.3168/jds.2019-18077 · Cited by 32 · see citing works

3-NOP: Mutagenicity and genotoxicity assessment

Thiel, A., Schoenmakers, A., Verbaan, I. A. J., Chenal, E., Etheve, S., & Beilstein, P. (2018) · Food and Chemical Toxicology · doi:10.1016/j.fct.2018.11.010 · Cited by 30 · see citing works

Short-Term Eating Preference of Beef Cattle Fed High Forage or High Grain Diets Supplemented with 3-Nitrooxypropanol

Lee, C., Kim, S., Beauchemin, K. A., Celi, P., & Duval, S. M. (2019) · Animals · doi:10.3390/ani10010064 · Cited by 17 · see citing works

Effects of DL-Malate on In Vitro Forage Fiber Digestion by Mixed Ruminal Microorganisms

Martin, S. A. (2004) · Current Microbiology · doi:10.1007/s00284-003-4081-x · Cited by 11 · see citing works

Methanogenesis and recent techniques for mitigation of methanogenesis in ruminants.

Bunglavan, S. J. (2014) · Journal of Livestock Science · Cited by 8 · see citing works

Technologies and perspectives for achieving carbon neutrality

Wang, F., Harindintwali, J. D., Yuan, Z., Wang, M., Wang, F., Li, S., Yin, Z., Huang, L., Fu, Y., Li, L., et al. (2021) · The Innovation · doi:10.1016/j.xinn.2021.100180 · Cited by 1,330 · see citing works

Full adoption of the most effective strategies to mitigate methane emissions by ruminants can help meet the 1.5 °C target by 2030 but not 2050

Arndt, C., Hristov, A. N., Price, W. J., McClelland, S. C., Pelaez, A. M., Cueva, S. F., Oh, J., Dijkstra, J., Bannink, A., Bayat, A. R., et al. (2022) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2111294119 · Cited by 391 · see citing works

Invited review: current enteric methane mitigation options

Beauchemin, K. A., Ungerfeld, E. M., Abdalla, A. L., Alvarez, C., Arndt, C., Becquet, P., Benchaar, C., Berndt, A., Mauricio, R. M., McAllister, T. A., et al. (2022) · Journal of Dairy Science · doi:10.3168/jds.2022-22091 · Cited by 324 · see citing works

Invited review: Advances in nutrition and feed additives to mitigate enteric methane emissions

Hristov, A. N. (2024) · Journal of Dairy Science · doi:10.3168/jds.2023-24440 · Cited by 95 · see citing works

A Review of 3-Nitrooxypropanol for Enteric Methane Mitigation from Ruminant Livestock

Yu, G., Beauchemin, K. A., & Dong, R. (2021) · Animals · doi:10.3390/ani11123540 · Cited by 89 · see citing works

Enteric methane mitigation interventions

Fouts, J. Q., Honan, M. C., Roque, B. M., Tricárico, J. M., & Kebreab, E. (2022) · Translational Animal Science · doi:10.1093/tas/txac041 · Cited by 68 · see citing works

Effects of dietary fat, nitrate, and 3-nitrooxypropanol and their combinations on methane emission, feed intake, and milk production in dairy cows

Maigaard, M., Weisbjerg, M. R., Johansen, M., Walker, N. D., Ohlsson, C., & Lund, P. (2023) · Journal of Dairy Science · doi:10.3168/jds.2023-23420 · Cited by 55 · see citing works

Feed additives for methane mitigation: Recommendations for testing enteric methane-mitigating feed additives in ruminant studies

Hristov, A. N., Bannink, A., Battelli, M., Belanche, A., Sanz, M. C. C., Fernández-Turren, G., García, F. J. G., Jonker, A., Kenny, D. A., Lind, V., et al. (2024) · Journal of Dairy Science · doi:10.3168/jds.2024-25050 · Cited by 52 · see citing works

Application of 3-nitrooxypropanol and canola oil to mitigate enteric methane emissions of beef cattle results in distinctly different effects on the rumen microbial community

Gruninger, R. J., Zhang, X., Smith, M. L., Kung, L., Vyas, D., McGinn, S. M., Kindermann, M., Wang, M., Tan, Z. L., & Beauchemin, K. A. (2022) · Animal Microbiome · doi:10.1186/s42523-022-00179-8 · Cited by 49 · see citing works

Current Perspectives on Achieving Pronounced Enteric Methane Mitigation From Ruminant Production

Ungerfeld, E. M., Beauchemin, K. A., & Muñoz, C. (2022) · Frontiers in Animal Science · doi:10.3389/fanim.2021.795200 · Cited by 42 · see citing works

Gas exchange, rumen hydrogen sinks, and nutrient digestibility and metabolism in lactating dairy cows fed 3-nitrooxypropanol and cracked rapeseed

Kjeldsen, M. H., Weisbjerg, M. R., Larsen, M., Højberg, O., Ohlsson, C., Walker, N. D., Hellwing, A. L. F., & Lund, P. (2023) · Journal of Dairy Science · doi:10.3168/jds.2023-23743 · Cited by 41 · see citing works

Mitigating enteric methane emissions: An overview of methanogenesis, inhibitors and future prospects

Xie, X., Cao, Y., Li, Q., Li, Q., Yang, X., Wang, R., Zhang, X., Tan, Z., Lin, B., & Wang, M. (2025) · Animal nutrition · doi:10.1016/j.aninu.2025.02.001 · Cited by 43 · see citing works

Invited review: Interplay of rumen microbiome and the cattle host in modulating feed efficiency and methane emissions

Badhan, A., Wang, Y., Terry, S. A., Gruninger, R. J., Guan, L. L., & McAllister, T. A. (2025) · Journal of Dairy Science · doi:10.3168/jds.2024-26063 · Cited by 45 · see citing works

Recent advances in feed additives with the potential to mitigate enteric methane emissions from ruminant livestock

Kelly, L., & Kebreab, E. (2023) · Journal of Soil and Water Conservation · doi:10.2489/jswc.2023.00070 · Cited by 33 · see citing works

Effect of 3-nitrooxypropanol on enteric methane emissions of feedlot cattle fed with a tempered barley-based diet with canola oil

Almeida, A. K., Cowley, F. C., McMeniman, J. P., Karagiannis, A., Walker, N. D., Tamassia, L. F. M., McGrath, J. J., & Hegarty, R. S. (2023) · Journal of Animal Science · doi:10.1093/jas/skad237 · Cited by 31 · see citing works

Review: Biological consequences of the inhibition of rumen methanogenesis

Ungerfeld, E. M., & Pitta, D. (2024) · animal · doi:10.1016/j.animal.2024.101170 · Cited by 31 · see citing works

Recent advances in feed and nutrition of beef cattle in China — A review

Gao, Q., Liu, H., Zuo, W., Lan, X., An, J., Shen, W., & Wan, F. (2022) · Animal Bioscience · doi:10.5713/ab.22.0192 · Cited by 33 · see citing works

Feed additives for methane mitigation: Modeling the impact of feed additives on enteric methane emission of ruminants—Approaches and recommendations

Dijkstra, J., Bannink, A., Congio, G. F. D. S., Ellis, J. L., Eugène, M., Garcia, F., Niu, M., Vibart, R. E., Yáñez-Ruíz, D. R., & Kebreab, E. (2024) · Journal of Dairy Science · doi:10.3168/jds.2024-25049 · Cited by 29 · see citing works

3-Nitrooxypropanol supplementation of a forage diet decreased enteric methane emissions from beef cattle without affecting feed intake and apparent total-tract digestibility

Alemu, A. W., Gruninger, R. J., Zhang, X., O’Hara, E., Kindermann, M., & Beauchemin, K. A. (2023) · Journal of Animal Science · doi:10.1093/jas/skad001 · Cited by 29 · see citing works

Quantifying the Impact of Different Dietary Rumen Modulating Strategies on Enteric Methane Emission and Productivity in Ruminant Livestock: A Meta-Analysis

Pepeta, B. N., Hassen, A., & Tesfamariam, E. H. (2024) · Animals · doi:10.3390/ani14050763 · Cited by 35 · see citing works

Effect of combining the methanogenesis inhibitor 3-nitrooxypropanol and cottonseeds on methane emissions, feed intake, and milk production of grazing dairy cows

Muñoz, C., Muñoz, I., Rodríguez, R., Urrutia, N. L., & Ungerfeld, E. M. (2024) · animal · doi:10.1016/j.animal.2024.101203 · Cited by 19 · see citing works

Effects of dietary supplementation with 3-nitrooxypropanol on enteric methane production, rumen fermentation, and performance in young growing beef cattle offered a 50:50 forage:concentrate diet

Kirwan, S. F., Tamassia, L. F. M., Walker, N. D., Karagiannis, A., Kindermann, M., & Waters, S. M. (2023) · Journal of Animal Science · doi:10.1093/jas/skad399 · Cited by 17 · see citing works

A Meta-Analysis of 3-Nitrooxypropanol Dietary Supplementation on Growth Performance, Ruminal Fermentation, and Enteric Methane Emissions of Beef Cattle

Orzuna‐Orzuna, J. F., Godina-Rodríguez, J. E., Garay-Martínez, J. R., Rivera, L. D. G., Maldonado‐Jáquez, J. A., & Lara-Bueno, A. (2024) · Fermentation · doi:10.3390/fermentation10060273 · Cited by 16 · see citing works

A Meta-Analysis of Dietary Inhibitors for Reducing Methane Emissions via Modulating Rumen Microbiota in Ruminants

Ma, G., Jin, W., Zhang, Y., Gai, Y., Tang, W. H. W., Guo, L., Azzaz, H. H., Ghaffari, M. H., Gu, Z., Mao, S., et al. (2024) · Journal of Nutrition · doi:10.1016/j.tjnut.2024.12.011 · Cited by 14 · see citing works

A review of key microbial and nutritional elements for mechanistic modeling of rumen fermentation in cattle under methane-inhibition

Pressman, E. M., & Kebreab, E. (2024) · Frontiers in Microbiology · doi:10.3389/fmicb.2024.1488370 · Cited by 17 · see citing works

International Symposium on Ruminant Physiology: Rumen fungi, archaea, and their interactions

McAllister, T. A., Thomas, K. D., Gruninger, R. J., Elshahed, M. S., Li, Y., & Cheng, Y. (2025) · Journal of Dairy Science · doi:10.3168/jds.2024-25713 · Cited by 14 · see citing works

Combined effects of nitrate and medium-chain fatty acids on methane production, rumen fermentation, and rumen bacterial populations in vitro

Vadroňová, M., Šťovíček, A., Jochová, K., Výborná, A., Tyrolová, Y., Tichá, D., Homolka, P., & Joch, M. (2023) · Scientific Reports · doi:10.1038/s41598-023-49138-6 · Cited by 12 · see citing works

The Effects of Different Doses of 3-NOP on Ruminal Fermentation Parameters, Methane Production, and the Microbiota of Lambs In Vitro

Xuan, T., Zheng, T., Li, T., Wu, B., Li, T. S., Bao, W., & Qin, W. (2024) · Fermentation · doi:10.3390/fermentation10090440 · Cited by 12 · see citing works

The Path to Net-Zero in Dairy Production: Are Pronounced Decreases in Enteric Methane Achievable?

Beauchemin, K. A., Kebreab, E., Cain, M. L., & VandeHaar, M. J. (2024) · Annual Review of Animal Biosciences · doi:10.1146/annurev-animal-010324-113703 · Cited by 13 · see citing works

Greenhouse-gas abatement on Australian dairy farms: what are the options?

Garnett, L., & Eckard, R. (2024) · Animal Production Science · doi:10.1071/an24139 · Cited by 11 · see citing works

Perspective: Could dairy cow nutrition meaningfully reduce the carbon footprint of milk production?

Hristov, A. N. (2023) · Journal of Dairy Science · doi:10.3168/jds.2023-23461 · Cited by 10 · see citing works

The influence of feeding canola oil steeped Asparagopsis armata on resulting fatty acid profile and dairy processing properties of cow’s milk

Alvarez-Hess, P. S., Thomson, A. L., Williams, S. R. O., Logan, A., Taylor, C. A., Singh, T. K., Roque, B. M., Neachtain, A. S. Ó., Kinley, R. D., & Jacobs, J. L. (2024) · Animal Feed Science and Technology · doi:10.1016/j.anifeedsci.2024.115924 · Cited by 8 · see citing works

Red seaweed supplementation suppresses methanogenesis in the rumen, revealing potentially advantageous traits among hydrogenotrophic bacteriaPreprint

Zhang, P., Roque, B. M., Romero, P., Shapiro, N., Eloe‐Fadrosh, E. A., Kebreab, E., Diamond, S., & Hess, M. (2024) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2024.06.07.597961 · Cited by 7 · see citing works

Effect of 3-Nitrooxypropanol Combined with Different Feed Additives on Growth Performance, Carcass Traits, Enteric Methane Emissions, and Physiological Responses in Feedlot Beef Cattle Fed a High-Concentrate Finishing Diet

de Souza, W. L., Niehues, M. B., Cardoso, A. D. S., de Carvalho, V. V., Perdigão, A., Acedo, T. S., Costa, D. F. A., Tamassia, L. F. M., Kindermann, M., & Reis, R. A. (2024) · Animals · doi:10.3390/ani14233488 · Cited by 6 · see citing works

Enteric methane emissions, rumen fermentation, and milk composition of dairy cows fed 3-nitrooxypropanol and L-malate supplements

Zhou, X., Fu, S., Li, G., Yao, Z., Du, X., Zhang, Y., & Gao, T. (2024) · Frontiers in Veterinary Science · doi:10.3389/fvets.2024.1479535 · Cited by 6 · see citing works

Red seaweed supplementation suppresses methanogenesis in the rumen, revealing potentially advantageous traits among hydrogenotrophic bacteria

Zhang, P., Roque, B. M., Romero, P., Shapiro, N., Eloe‐Fadrosh, E. A., Kebreab, E., Diamond, S., & Hess, M. (2025) · Microbiome · doi:10.1186/s40168-025-02251-2 · Cited by 5 · see citing works

Effect of Cymbopogon citratus on Enteric Methane Emission, Nutrients Digestibility, and Energy Partition in Growing Beef Cattle

Vázquez-Carrillo, M. F., Zaragoza-Guerrero, R., Corona, L., González-Ronquillo, M., Castillo-Gallegos, E., & Ortega, O. A. C. (2023) · Agriculture · doi:10.3390/agriculture13040745 · Cited by 4 · see citing works

Loss of Myostatin leads to low production of CH4 by altering rumen microbiota and metabolome in cattle

Hai, C., Wang, L., Wu, D., Pei, D., Yang, Y., Liu, X., Zhao, Y., Bai, C., Su, G., Bao, Z., et al. (2025) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2025.139533 · Cited by 3 · see citing works

Understanding heterogeneity in methane emissions from confinement-fed dairy and beef cattle supplemented with Bovaer®: a meta-analysis

Macdonald, A., Shephard, R. W., Hepworth, G., & Eckard, R. (2025) · Frontiers in Animal Science · doi:10.3389/fanim.2025.1689264 · Cited by 3 · see citing works

A meta-analysis of 3-nitrooxypropanol effects on methane production and yield in beef cattle

de Oliveira, M. H., Zihlmann, R., Bannink, A., Beauchemin, K. A., Dijkstra, J., Pressman, E. M., van Gastelen, S., & Kebreab, E. (2026) · animal · doi:10.1016/j.animal.2026.101767 · Cited by 3 · see citing works

Insights into Effects of Combined Capric and Lauric Acid on Rumen Bacterial Composition

Vadroňová, M., Šťovíček, A., Výborná, A., Tyrolová, Y., Tichá, D., & Joch, M. (2024) · Microorganisms · doi:10.3390/microorganisms12061085 · Cited by 3 · see citing works

Toward compatibility with national dairy production and climate goals through locally appropriate mitigation interventions in Kenya

Graham, M. W., Özkan, Ş., Arndt, C., González-Quintero, R., Korir, D., Merbold, L., Mottet, A., Ndung’u, P. W., Notenbaert, A. M. O., & Leitner, S. (2024) · Agricultural Systems · doi:10.1016/j.agsy.2024.104098 · Cited by 3 · see citing works

Assessing the Impact of the Methane Inhibitors 3-Nitrooxypropanol (3-NOP) and Canola Oil on the Rumen Anaerobic Fungi

O’Hara, E., Chomistek, N., Terry, S. A., Beauchemin, K. A., & Gruninger, R. J. (2025) · Animals · doi:10.3390/ani15091230 · Cited by 3 · see citing works

Short- and long-term dietary supplementation as well as withdrawal of the enteric methane inhibitor 3-nitrooxypropanol reveal distinct effects on the rumen microbial community

Choi, Y., Zhou, M., Romero-Pérez, A., Beauchemin, K. A., Duval, S. M., Kindermann, M., & Guan, L. L. (2025) · Journal of Animal Science and Biotechnology/Journal of animal science and biotechnology · doi:10.1186/s40104-025-01291-w · Cited by 2 · see citing works

Combination of carbohydrases and essential oils improve dietary performance of feedlot steers on a high-energy diet

Abreu, J. A. D. D. C., Neumann, M., Paris, W., de Souza, A. M., Vigne, G. L. D., de Almeida, E. R., Cristo, F. B., & Sidor, F. D. S. (2021) · Semina Ciências Agrárias · doi:10.5433/1679-0359.2022v43n2p523 · Cited by 2 · see citing works

Nutritional Manipulation as a Strategy to Mitigate Enteric Methane Emissions from Livestock – A Review

Dayoub, W. (2024) · International Journal of Agriculture and Biology · doi:10.17957/ijab/15.2235 · Cited by 4 · see citing works

Lactational performance and enteric methane emissions in dairy cows fed high-oil oats, cold-pressed rapeseed cake, and 3-nitrooxypropanol in a grass silage–based diet

Fant, P., Mantovani, G., Vadroňová, M., Sabetti, M. C., Krizsan, S. J., & Ramin, M. (2025) · Journal of Dairy Science · doi:10.3168/jds.2025-27007 · Cited by 2 · see citing works

Impact of Zootechnical Additive of the Nitrooxypropanol to Minimize Methane Emission in Cattle: A Review

Mshary, G. S., Castillo, C., Bermúdez, J. H., & Muíño, R. (2024) · Diyala Agricultural Sciences Journal · doi:10.52951/dasj.24160203 · Cited by 1 · see citing works

Effect of Hemp Seed Oil on Milk Performance, Blood Parameters, Milk Fatty Acid Profile, and Rumen Microbial Population in Milk-Producing Buffalo: Preliminary Study

Gu, Q., Lin, B., Wan, D., Kong, Z., Tang, Q., Qi, Y., Cai, X., Ding, H., Qin, G., & Zou, C. (2025) · Animals · doi:10.3390/ani15040514 · Cited by 1 · see citing works

Investigating interactions between grass silage– or corn silage–based diets and 3-nitrooxypropanol on fermentation and methane emission dynamics and rumen microbiome in dairy cattle

van Gastelen, S., Dijkstra, J., Alferink, S. J. J., Binnendijk, G. P., Klop, A., van Themaat, E. V. L., Walker, N. D., & Bannink, A. (2025) · Journal of Dairy Science · doi:10.3168/jds.2025-26957 · Cited by 1 · see citing works

Effects of dietary fat supplementation on enteric methane mitigation and farm profitability: A case study of an organic and a conventional dairy herd

Jørgensen, N., Chen, L., Thorup, V. M., Weisbjerg, M. R., & Østergaard, S. (2025) · Livestock Science · doi:10.1016/j.livsci.2025.105856 · Cited by 1 · see citing works

Inclusion of rapeseed cake or oil in the diet of beef cattle reduces enteric methane emissions without affecting animal performance

Folliard, N. T., Waters, S. M., Roskam, E., O’Flaherty, V., & Kenny, D. A. (2026) · animal · doi:10.1016/j.animal.2026.101780 · Cited by 1 · see citing works

Advancing methane mitigation in ruminants through feed additive combinations: efficacy, interactions, and implementation challenges

Ramos‐Morales, E., Palma‐Hidalgo, J. M., Newbold, J. R., Pardo, Z., Yáñez-Ruíz, D. R., & Newbold, C. J. (2026) · Frontiers in Microbiology · doi:10.3389/fmicb.2026.1683471 · Cited by 1 · see citing works

Evaluación del efecto de Cymbopogon citratus sobre el consumo, digestibilidad, emisión de metano y partición de la energía en bovinos en crecimiento

Vázquez-Carrillo, M. F., Zaragoza-Guerrero, R., Corona, L., González-Ronquillo, M., Castillo-Gallegos, E., & Ortega, O. A. C. (2023) · Archivos Latinoamericanos de Producción Animal · doi:10.53588/alpa.310537

Hemp Seed Oil Can Improve Serum Antioxidant Capacity and Regulate Blood Lipid and Milk Fatty Acid Metabolism in Dairy Water BuffaloPreprint

Gu, Q., Lin, B., Wan, D., Tang, Q., Qi, Y., Ding, H., Zou, C., & Qin, G. (2024) · SSRN Electronic Journal · doi:10.2139/ssrn.4822318

Livestock as a source of greenhouse gas emission

Vázquez-Carrillo, M. F., Jimenez, L. E. R., Radic‐Schilling, S., Ortega, O. A. C., & González-Ronquillo, M. (2024) · Elsevier eBooks · doi:10.1016/b978-0-443-19231-9.00004-1

The effect of increasing canola oil and soybean oil addition to beef cattle rations based on corn silage and barley grain on the in vitro ruminal gas production and rumen fermentation

Kara, K., Yılmaz, K., Öztaş, S. Y., & Pi̇rci̇, G. (2024) · Grassland Science · doi:10.1111/grs.12432

Supplementation of Oils or Oilseeds as Methane-Mitigating Feed Components in Dairy and Beef Cattle: A Meta-AnalysisPreprint

Malik, M. I., Capucchio, M. T., Rashid, M. A., Salami, S. A., Zhang, Q., Cheng, L., & Hao, L. (2024) · SSRN Electronic Journal · doi:10.2139/ssrn.5064872

Metatranscriptomic changes in the propionate pathway and carbohydrate enzyme revealed the rebalancing mechanism of rumen microbiota after CH4 inhibition by 3-NOPPreprint

Cao, Y., Zhang, S., Li, Q., Zhou, X., Wang, W., Ouyang, B., Zhang, X., Tan, Z., & Wang, M. (2025) · Research Square · doi:10.21203/rs.3.rs-7121971/v1

Twice daily feeding of two phytogenic dietary additives; their individual and combined effects on methane production, dry matter intake, milk production and composition in dairy cows

Adam, C., Dando, I., Berkhout, M., Neachtain, A. S. Ó., Ho, C. K. M., Jacobs, J. L., Giri, K., Williams, S. R. O., & Marett, L. C. (2025) · Animal Feed Science and Technology · doi:10.1016/j.anifeedsci.2025.116559

Economic and circular economy analysis of including potato waste in beef feedlot diets: a Canadian case study

Stephens, E. C., Aboagye, I. A., Mengistu, G. F., McAllister, T. A., & Ominski, K. (2025) · Frontiers in Sustainability · doi:10.3389/frsus.2025.1628164

In vitro dose-response of bromoform stabilized in vegetable oil on rumen fermentation and methane production

Gonzalez-Chappe, L., Daudet, A., Firkins, J. L., Relling, A. E., & Pittaluga, A. M. (2026) · JDS Communications · doi:10.3168/jdsc.2025-0988

Potential of faecal NIRS for predicting methane emissions in dairy cows supplemented with 3-nitrooxypropanol

Soares, D., Baleret, N., Parra, D., Martin, C., & Andueza, D. (2026) · animal · doi:10.1016/j.animal.2026.101913

3-Nitrooxypropanol as a rumen methane mitigation agent: mechanisms, efficacy, and future directions

Zeeshan, M., Khan, N. A., Zhang, X., Liu, Y., & Tu, Y. (2026) · Circular Agricultural Systems · doi:10.48130/cas-0026-0017

Use of 3-nitrooxypropanol in a commercial feedlot to decrease enteric methane emissions from cattle fed a corn-based finishing diet

Alemu, A. W., Shreck, A. L., Booker, C. W., McGinn, S. M., Pekrul, L. K. D., Kindermann, M., & Beauchemin, K. A. (2020) · Journal of Animal Science · doi:10.1093/jas/skaa394 · Cited by 31 · see citing works

330 3-nitrooxypropanol Supplementation of a Forage Diet Decreased Enteric Methane Emissions from Beef Cattle Without Affecting Apparent Total-tract Digestibility

Alemu, A. W., Zhang, X., Kindermann, M., & Beauchemin, K. A. (2021) · Journal of Animal Science · doi:10.1093/jas/skab235.338 · Cited by 7 · see citing works

Effect of 3-nitrooxypropanol on enteric methane emissions of feedlot cattle fed with a tempered barley-based diet with canola oil

Almeida, A. K., Cowley, F. C., McMeniman, J. P., Karagiannis, A., Walker, N. D., Tamassia, L. F. M., McGrath, J. J., & Hegarty, R. S. (2023) · Journal of Animal Science · doi:10.1093/jas/skad237 · Cited by 31 · see citing works

Effects of 3-nitrooxypropanol on methane emissions, rumen fermentation, and performance of Hanwoo steers fed an early fattening stage diet

Bharanidharan, R., Tomple, B. M., Tamassia, L. F. M., Hong, W. H., Cho, J. Y., Zihlmann, R., Kindermann, M., Hong, S., & Kim, K. H. (2026) · Journal of Animal Science · doi:10.1093/jas/skag096

The potential of 3-nitrooxypropanol to lower enteric methane emissions from beef cattle1

Romero-Pérez, A., Okine, E. K., McGinn, S. M., Guan, L. L., Oba, M., Duval, S. M., Kindermann, M., & Beauchemin, K. A. (2014) · Journal of Animal Science · doi:10.2527/jas.2014-7573 · Cited by 113 · see citing works