An inhibitor persistently decreased enteric methane emission from dairy cows with no negative effect on milk production
Open accessAbstract
A quarter of all anthropogenic methane emissions in the United States are from enteric fermentation, primarily from ruminant livestock. This study was undertaken to test the effect of a methane inhibitor, 3-nitrooxypropanol (3NOP), on enteric methane emission in lactating Holstein cows. An experiment was conducted using 48 cows in a randomized block design with a 2-wk covariate period and a 12-wk data collection period. Feed intake, milk production, and fiber digestibility were not affected by the inhibitor. Milk protein and lactose yields were increased by 3NOP. Rumen methane emission was linearly decreased by 3NOP, averaging about 30% lower than the control. Methane emission per unit of feed dry matter intake or per unit of energy-corrected milk were also about 30% less for the 3NOP-treated cows. On average, the body weight gain of 3NOP-treated cows was 80% greater than control cows during the 12-wk experiment. The experiment demonstrated that the methane inhibitor 3NOP, applied at 40 to 80 mg/kg feed dry matter, decreased methane emissions from high-producing dairy cows by 30% and increased body weight gain without negatively affecting feed intake or milk production and composition. The inhibitory effect persisted over 12 wk of treatment, thus offering an effective methane mitigation practice for the livestock industries.
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3-nitrooxypropanol is a promising methanogenesis inhibitor that reduces methane emissions in ruminant animals by up to 40%.
“3-nitrooxypropanol is the latest developed and promising inhibitor for methanogenesis,127 which has been shown to reduce methane emissions in ruminant animals by up to 40%.128,129”
From Technologies and perspectives for achieving carbon neutrality (2021) · cites this paper as [128]
3-nitrooxypropanol (3-NOP) reduces methane emissions by up to 30%.
“3-nitrooxypropanol (3-NOP) has been shown to reduce methane emissions by up to 30% (Hristov et al., 2015; Jayanegara et al., 2018).”
From Addressing global ruminant agricultural challenges through understanding the rumen microbiome: past, present, and future (2018) · cites this paper as Hristov et al., 2015
Inhibition of methyl-coenzyme M reductase can result in a reduction in rumen methanogenesis (up to 30%) without negative effects to the animal.
“This enzyme catalyzes the last step of methane-forming reactions (Duin et al., 2016) and its inhibition can result in a reduction in rumen methanogenesis (up to 30%) without negative effects to the animal (Hristov et al., 2015).”
From Addressing global ruminant agricultural challenges through understanding the rumen microbiome: past, present, and future (2018) · cites this paper as Hristov et al., 2015
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Estimación lineal de los requerimientos nutricionales del NRC para ganado de leche.
Elizondo-Salazar, J. A. (2014) · Agronomía Mesoamericana · doi:10.15517/am.v13i1.13244 · Cited by 6,497 · see citing works
Inventory of U.S. Greenhouse Gas Emissions and Sinks
Hockstad, L., & Hanel, L. (2018) · University of North Texas Digital Library (University of North Texas) · doi:10.15485/1464240 · Cited by 2,861 · see citing works
Johnson, K. A., & Johnson, D. E. (1995) · Journal of Animal Science · doi:10.2527/1995.7382483x · Cited by 2,714 · cited 1× · see citing works
“Apart from its GHG effect, the emission of enteric methane is a net loss of feed energy (25), which cannot be used by the ruminant animal for productive purposes.”
BackgroundN UTRITIONAL R EGULATION OF M ILK F AT S YNTHESIS
Bauman, D. E., & Griinari, J. M. (2003) · Annual Review of Nutrition · doi:10.1146/annurev.nutr.23.011702.073408 · Cited by 1,167 · see citing works
Knapp, J. R., Laur, G. L., Vadas, P. A., Weiss, W. P., & Tricarico, J. M. (2014) · Journal of Dairy Science · doi:10.3168/jds.2013-7234 · Cited by 1,002 · see citing works
The World Intellectual Property Organization
Foltea, M. (2012) · Cambridge University Press eBooks · doi:10.1017/cbo9781139235969.011 · Cited by 885 · see citing works
Janssen, P. H. (2010) · Animal Feed Science and Technology · doi:10.1016/j.anifeedsci.2010.07.002 · Cited by 848 · see citing works
Factors affecting odd- and branched-chain fatty acids in milk: A review
Vlaeminck, B., Fievez, V., Cabrita, A. R. J., Fonseca, A. J. M., & Dewhurst, R. J. (2006) · Animal Feed Science and Technology · doi:10.1016/j.anifeedsci.2006.06.017 · Cited by 750 · see citing works
A Nordic proposal for an energy corrected milk (ECM) formula
Sjaunja, L.-O., Baevre, L., Junkkarinen, L., Pedersen, J., & Setala, J. (1991) · Cited by 616 · see citing works
Crystal Structure of Methyl-Coenzyme M Reductase: The Key Enzyme of Biological Methane Formation
Ermler, U., Grabarse, W., Shima, S., Goubeaud, M., & Thauer, R. K. (1997) · Science · doi:10.1126/science.278.5342.1457 · Cited by 608 · see citing works
Anthropogenic emissions of methane in the United States
Miller, S., Wofsy, S., Michalak, A., Kort, E., Andrews, A., Biraud, S., Dlugokencky, E., Eluszkiewicz, J., Fischer, M., Janssens-Maenhout, G., et al. (2013) · Proceedings of the National Academy of Sciences of the United States of America · doi:10.1073/pnas.1314392110 · Cited by 541 · see citing works
Johnson, K., Huyler, M. T., Westberg, H. H., Lamb, B., & Zimmerman, P. (1994) · Environmental Science & Technology · doi:10.1021/es00051a025 · Cited by 532 · see citing works
Huhtanen, P. J., Kaustell, K., & Jaakkola, S. (1994) · Animal Feed Science and Technology · doi:10.1016/0377-8401(94)90173-2 · Cited by 524 · see citing works
Redirecting rumen fermentation to reduce methanogenesis
McAllister, T. A., & Newbold, C. J. (2008) · Australian Journal of Experimental Agriculture · doi:10.1071/ea07218 · Cited by 501 · see citing works
Effects of essential oils on ruminal microorganisms and their protein metabolism
McIntosh, F. M., Williams, P., Losa, R., Wallace, R. J., Beever, D. A., & Newbold, C. J. (2003) · Applied and Environmental Microbiology · doi:10.1128/aem.69.8.5011-5014.2003 · Cited by 412 · see citing works
Ragsdale, S. W. (2009) · Journal of Biological Chemistry · doi:10.1074/jbc.r900020200 · Cited by 400 · see citing works
Greenhouse gas emissions from ruminant supply chains – a global life cycle assessment
Opio, C., Gerber, P., Mottet, A., Falcucci, A., Tempio, G., MacLeod, M. J., Vellinga, T. V., Henderson, B. B., & Steinfeld, H. (2013) · Cited by 396 · see citing works
Ruminant enteric methane mitigation: a review
Cottle, D., Nolan, J. V., & Wiedemann, S. G. (2011) · Animal Production Science · doi:10.1071/an10163 · Cited by 343 · see citing works
Leahy, S. C., Kelly, W. J., Altermann, E., Ronimus, R. S., Yeoman, C. J., Pacheco, D. M., Li, D., Kong, Z., McTavish, S. M., Sang, C., et al. (2010) · PLoS ONE · doi:10.1371/journal.pone.0008926 · Cited by 318 · see citing works
Milk odd- and branched-chain fatty acids as biomarkers of rumen function—An update
Fievez, V., Colman, E., Castro-Montoya, J. M., Stefanov, I., & Vlaeminck, B. (2011) · Animal Feed Science and Technology · doi:10.1016/j.anifeedsci.2011.12.008 · Cited by 304 · see citing works
Hristov, A. N., Oh, J.-A., Lee, C., Meinen, R. J., Montes, F., OTT, T. L., Firkins, J. L., Rotz, A. C., Dell, C. J., Adesogan, C., et al. (2013) · Socio-Environmental Systems Modeling · Cited by 303 · see citing works
Development of equations for predicting methane emissions from ruminants
Ramin, M., & Huhtanen, P. J. (2013) · Journal of Dairy Science · doi:10.3168/jds.2012-6095 · Cited by 301 · see citing works
Ungerfeld, E. M. (2015) · Frontiers in Microbiology · doi:10.3389/fmicb.2015.00037 · Cited by 269 · see citing works
Milk Fat: Origin of Fatty Acids and Influence of Nutritional Factors Thereon
Palmquist, D. L. (2007) · Springer eBooks · doi:10.1007/0-387-28813-9_2 · Cited by 227 · see citing works
Hydrogen as an intermediate in the rumen fermentation
Hungate, R. E. (1967) · Archives of Microbiology · doi:10.1007/bf00406327 · Cited by 224 · see citing works
Wecht, K. J., Jacob, D. J., Frankenberg, C., Jiang, Z., & Blake, D. R. (2014) · Journal of Geophysical Research Atmospheres · doi:10.1002/2014jd021551 · Cited by 201 · see citing works
Yan, T., Mayne, C. S., Gordon, F. G., Porter, M. G., Agnew, R. E., Patterson, D. C., Ferris, C. P., & Kilpatrick, D. J. (2010) · Journal of Dairy Science · doi:10.3168/jds.2009-2929 · Cited by 193 · see citing works
The Rumen Fermentation: A Model for Microbial Interactions in Anaerobic Ecosystems
Wolin, M. J. (1979) · Advances in microbial ecology · doi:10.1007/978-1-4615-8279-3_2 · Cited by 173 · see citing works
Reynolds, C. K., Humphries, D. J., Kirton, P., Kindermann, M., Duval, S. M., & Steinberg, C. E. W. (2014) · Journal of Dairy Science · doi:10.3168/jds.2013-7397 · Cited by 159 · see citing works
Relationships between methane production and milk fatty acid profiles in dairy cattle
Dijkstra, J., van Zijderveld, S. M., Apajalahti, J., Bannink, A., Gerrits, W. J. J., Newbold, J. R., Perdok, H. B., & Berends, H. (2011) · Animal Feed Science and Technology · doi:10.1016/j.anifeedsci.2011.04.042 · Cited by 152 · see citing works
Mitsumori, M., Shinkai, T., Takénaka, A., Enishi, O., Higuchi, K., Kobayashi, Y., Nonaka, I., Asanuma, N., Denman, S. E., & McSweeney, C. S. (2011) · British Journal Of Nutrition · doi:10.1017/s0007114511005794 · Cited by 147 · see citing works
Lopes, J. C., de Matos, L. F., Harper, M. T., Giallongo, F., Oh, J., Gruen, D. S., Ono, S., Kindermann, M., Duval, S. M., & Hristov, A. N. (2016) · Journal of Dairy Science · doi:10.3168/jds.2015-10832 · Cited by 147 · see citing works
Haisan, J., Sun, Y., Guan, L. L., Beauchemin, K. A., Iwaasa, A. D., Duval, S. M., Barreda, D. R., & Oba, M. (2014) · Journal of Dairy Science · doi:10.3168/jds.2013-7834 · Cited by 146 · see citing works
Wang, M., Sun, X., Janssen, P. H., Tang, S., & Tan, Z. (2014) · Animal Feed Science and Technology · doi:10.1016/j.anifeedsci.2014.04.012 · Cited by 145 · see citing works
Hristov, A. N., Lee, C., Cassidy, T. W., Long, M., Heyler, K. S., Corl, B. A., & Forster, R. J. (2010) · Journal of Dairy Science · doi:10.3168/jds.2010-3508 · Cited by 141 · see citing works
Martı́nez, G., Abecia, L., Arco, A., Cantalapiedra-Hijar, G., Martín-García, A. I., Molina‐Alcaide, E., Kindermann, M., Duval, S. M., & Yáñez-Ruíz, D. R. (2014) · Journal of Dairy Science · doi:10.3168/jds.2013-7398 · Cited by 140 · see citing works
Hristov, A. N., Oh, J., Giallongo, F., Frederick, T. W., Weeks, H. L., ZIMMERMAN, P. R., Harper, M. T., Hristova, R. A., Zimmerman, R. S., & Branco, A. F. (2015) · Journal of Visualized Experiments · doi:10.3791/52904 · Cited by 138 · see citing works
Hristov, A. N., Lee, C., Cassidy, T. W., Heyler, K. S., Tekippe, J., Varga, G. A., Corl, B. A., & Brandt, R. C. (2012) · Journal of Dairy Science · doi:10.3168/jds.2012-5975 · Cited by 130 · see citing works
Romero-Pérez, A., Okine, E. K., McGinn, S. M., Guan, L., Oba, M., Duval, S. M., Kindermann, M., & Beauchemin, K. A. (2015) · Journal of Animal Science · doi:10.2527/jas.2014-8726 · Cited by 128 · see citing works
The potential of 3-nitrooxypropanol to lower enteric methane emissions from beef cattle1
Romero-Pérez, A., Okine, E. K., McGinn, S. M., Guan, L. L., Oba, M., Duval, S. M., Kindermann, M., & Beauchemin, K. A. (2014) · Journal of Animal Science · doi:10.2527/jas.2014-7573 · Cited by 113 · see citing works
Lee, C., & Hristov, A. N. (2013) · Journal of Dairy Science · doi:10.3168/jds.2012-6442 · Cited by 112 · see citing works
Williams, S. R. O., Moate, P. J., Hannah, M. C., Ribaux, B. E., Wales, W. J., & Eckard, R. (2011) · Animal Feed Science and Technology · doi:10.1016/j.anifeedsci.2011.08.013 · Cited by 107 · see citing works
Deighton, M. H., Williams, S. R. O., Hannah, M. C., Eckard, R., Boland, T. M., Wales, W. J., & Moate, P. J. (2014) · Animal Feed Science and Technology · doi:10.1016/j.anifeedsci.2014.08.003 · Cited by 106 · see citing works
Diversity of gut methanogens in herbivorous animals.
St-Pierre, B., & Wright, A.-D. G. (2013) · Animal · doi:10.1017/s1751731112000912 · Cited by 98 · see citing works
Klevenhusen, F., Zeitz, J. O., Duval, S. M., Kreuzer, M., & Soliva, C. R. (2011) · Animal Feed Science and Technology · doi:10.1016/j.anifeedsci.2011.04.071 · Cited by 96 · see citing works
Soliva, C. R., Amelchanka, S. L., Duval, S. M., & Kreuzer, M. (2011) · British Journal Of Nutrition · doi:10.1017/s0007114510005684 · Cited by 90 · see citing works
Knight, T. W., Ronimus, R. S., Dey, D., Tootill, C. M., Naylor, G. E., Evans, P. N., Molano, G., Smith, A. H., Tavendale, M. H., Pinares-Patiño, C. S., et al. (2011) · Animal Feed Science and Technology · doi:10.1016/j.anifeedsci.2011.04.059 · Cited by 87 · see citing works
A meta-analysis of variability in continuous-culture ruminal fermentation and digestibility data
Hristov, A. N., Lee, C., Hristova, R., Huhtanen, P. J., & Firkins, J. L. (2012) · Journal of Dairy Science · doi:10.3168/jds.2012-5533 · Cited by 87 · see citing works
Holliger, C., Pierik, A. J., Reijerse, E. J., & Hagen, W. R. (1993) · Journal of the American Chemical Society · doi:10.1021/ja00066a034 · Cited by 84 · see citing works
van Lingen, H. J., Crompton, L. A., Hendriks, W. H., Reynolds, C. K., & Dijkstra, J. (2014) · Journal of Dairy Science · doi:10.3168/jds.2014-8268 · Cited by 77 · see citing works
Influence of Methane Inhibition on Energetic Efficiency of Lambs
Trei, J. E., Scott, G. C., & Parish, R. C. (1972) · Journal of Animal Science · doi:10.2527/jas1972.343510x · Cited by 64 · see citing works
Effect of Methane Inhibitors on Rumen Metabolism and Feedlot Performance of Sheep
Trei, J. E., Parish, R. C., Singh, Y. K., & Scott, G. C. (1971) · Journal of Dairy Science · doi:10.3168/jds.s0022-0302(71)85882-4 · Cited by 61 · see citing works
Scheller, S., Goenrich, M., Mayr, S., Thauer, R. K., & Jaun, B. (2010) · Angewandte Chemie International Edition · doi:10.1002/anie.201003214 · Cited by 51 · see citing works
Lee, C., Oh, J., Hristov, A. N., Harvatine, K. J., Vazquez-Anon, M., & Zanton, G. I. (2014) · Journal of Dairy Science · doi:10.3168/jds.2014-8904 · Cited by 36 · see citing works
Hristov, A. N., Oh, J., Giallongo, F., Frederick, T. W., Harper, M. T., Weeks, H. L., Branco, A. F., Price, W. J., Moate, P. J., Deighton, M. H., et al. (2016) · Journal of Dairy Science · doi:10.3168/jds.2016-10897 · Cited by 35 · see citing works
An investigation of possible competing mechanisms for Ni-containing methyl–coenzyme M reductase
Chen, S., Blomberg, M. R. A., & Siegbahn, P. E. M. (2014) · Physical Chemistry Chemical Physics · doi:10.1039/c4cp01483a · Cited by 34 · see citing works
Williams, S. R. O., Moate, P. J., Deighton, M. H., Hannah, M. C., & Wales, W. J. (2014) · Animal Production Science · doi:10.1071/an14292 · Cited by 29 · see citing works
Livestock methane emissions in the United States
Hristov, A. N., Johnson, K. A., & Kebreab, E. (2014) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1401046111 · Cited by 26 · see citing works
Flagged first, then by citations
1 of the 59 references couldn’t be matched to a known paper.
Research works printed but not linked: 5
- IPCC, Intergovernmental Panel on Climate Change (2014) Working Group III -Mitigation of Climate Change. Chapter 11, Agriculture, Forestry and Other Land Use (AFOLU) (Cambridge Univ Press, Cambridge, UK).
- McAllister TA, Newbold CJ (2008) Redirecting rumen methane to reduce methano- genesis. Aust J Exp Agric 48(2):7-13.
- National Research Council (2001) Nutrient Requirements of Dairy Cattle. 7th Revised Edition (National Academy of Sciences Press, Washington, DC).
- Hammond KJ, et al. (2013) Methane emissions from growing dairy heifers estimated using an automated head chamber (GreenFeed) compared to respiration chambers or SF 6 techniques. Adv Anim Biosci 4(2):391.
- Huhtanen P, Krizsan S, Cabezas Garcia EH, Hetta M, Gidlund H (2013) Repeatability and between cow variability of enteric CH 4 and total CO 2 emissions. Adv Anim Biosci 4(2):588.
Cited by
Sorted by citations
Technologies and perspectives for achieving carbon neutrality
Wang, F., Harindintwali, J. D., Yuan, Z., Wang, M., Wang, F., Li, S., Yin, Z., Huang, L., Fu, Y., Li, L., et al. (2021) · The Innovation · doi:10.1016/j.xinn.2021.100180 · Cited by 1,330 · see citing works
“Among these, 3-nitrooxypropanol is the latest developed and promising inhibitor for methanogenesis,(127) which has been shown to reduce methane emissions in ruminant animals by up to 40%.(128,129) Vaccination, by inducing the host immune system to create antibodies capable of suppressing methanogens, has the potential…”
BackgroundGreenhouse gas mitigation potentials in the livestock sector
Herrero, M., Henderson, B., Havlík, P., Thornton, P. K., Conant, R. T., Smith, P., Wirsenius, S., Hristov, A. N., Gerber, P., Gill, M., et al. (2016) · Nature Climate Change · doi:10.1038/nclimate2925 · Cited by 1,045 · see citing works
Self-citationBeauchemin, K. A., Ungerfeld, E. M., Eckard, R. J., & Wang, M. (2020) · Animal · doi:10.1017/s1751731119003100 · Cited by 619 · see citing works
“, 2014) and sustained decrease of CH4 production over several months have been reported for lactating dairy cows (25% to 32%, 12-week study; Hristov et al., 2015) and growing beef cattle (high-forage diet for 105 days, 37% decrease; highgrain diet for 105 days, 42% decrease; Vyas et al.”
BackgroundMethodThe rumen microbiome: balancing food security and environmental impacts
Mizrahi, I., Wallace, R. J., & Moraïs, S. (2021) · Nature Reviews Microbiology · doi:10.1038/s41579-021-00543-6 · Cited by 490 · see citing works
Huws, S. A., Creevey, C. J., Oyama, L. B., Mizrahi, I., Denman, S. E., Popova, M., Muñoz-Tamayo, R., Forano, E., Waters, S. M., Hess, M., et al. (2018) · Frontiers in Microbiology · doi:10.3389/fmicb.2018.02161 · Cited by 487 · see citing works
“, 2016) and its inhibition can result in a reduction in rumen methanogenesis (up to 30%) without negative effects to the animal (Hristov et al., 2015).”
BackgroundMatthews, C., Crispie, F., Lewis, E., Reid, M., O’Toole, P. W., & Cotter, P. D. (2018) · Gut Microbes · doi:10.1080/19490976.2018.1505176 · Cited by 466 · see citing works
“Recent studies have investigated the use of 3-Nitrooxypropanol (3NOP) to inhibit methane production.(104,105) In a study carried out by,(105) rumen methane emissions were decreased linearly by 30% through the use of 3NOP, while increasing milk protein and lactose yields.”
BackgroundInterpreting contemporary trends in atmospheric methane
Turner, A. J., Frankenberg, C., & Kort, E. A. (2019) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1814297116 · Cited by 426 · see citing works
Roque, B. M., Venegas, M., Kinley, R. D., de Nys, R., Duarte, T. L., Yang, X., & Kebreab, E. (2021) · PLOS ONE · doi:10.1371/journal.pone.0247820 · Cited by 433 · see citing works
The ruminal microbiome associated with methane emissions from ruminant livestock
Tapio, I., Snelling, T. J., Strozzi, F., & Wallace, R. J. (2017) · Journal of Animal Science and Biotechnology · doi:10.1186/s40104-017-0141-0 · Cited by 391 · see citing works
Ocko, I., Sun, T., Shindell, D. T., Oppenheimer, M., Hristov, A. N., Pacala, S. W., Mauzerall, D. L., Xu, Y., & Hamburg, S. P. (2021) · Environmental Research Letters · doi:10.1088/1748-9326/abf9c8 · Cited by 389 · see citing works
“…methane emissions from all major emitting sectors—such as the straightforward plugging of natural gas leaks (IEA 2017) to ruminant feed supplements (Hristov et al 2015)—and the resulting abatement potentials for readily available measures have been characterized (EPA 2013, 2019, IEA 2017, Harmsen…”
BackgroundRoque, B. M., Salwen, J. K., Kinley, R., & Kebreab, E. (2019) · Journal of Cleaner Production · doi:10.1016/j.jclepro.2019.06.193 · Cited by 364 · see citing works
Roehe, R., Dewhurst, R. J., Duthie, C.-A., Rooke, J. A., McKain, N., Ross, D. W., Hyslop, J. J., Waterhouse, A., Freeman, T. C., Watson, M., et al. (2016) · PLoS Genetics · doi:10.1371/journal.pgen.1005846 · Cited by 359 · see citing works
Reducing emissions from agriculture to meet the 2 °C target
Wollenberg, E. K., Richards, M., Smith, P., Havlík, P., Obersteiner, M. H., Tubiello, F. N., Herold, M., Gerber, P., Carter, S., Reisinger, A. R., et al. (2016) · Global Change Biology · doi:10.1111/gcb.13340 · Cited by 363 · see citing works
“Promising options include recently developed methane inhibitors that reduce dairy cow emissions by 30% while increasing body weight without affecting milk yields or composition (Hristov et al., 2015).”
BackgroundNiu, M., Kebreab, E., Hristov, A. N., Oh, J., Arndt, C., Bannink, A., Bayat, A. R., Brito, A. F., Boland, T. M., Casper, D. P., et al. (2018) · Global Change Biology · doi:10.1111/gcb.14094 · Cited by 343 · see citing works
“, 2016), 3nitrooxypropanol (Hristov et al., 2015), and intragastric infusion of acetate, propionate, glucose, and cis- or trans-fatty acids.”
Duin, E. C., Wagner, T., Shima, S., Prakash, D., Cronin, B., Yáñez-Ruiz, D. R., Duval, S., Rümbeli, R., Stemmler, R. T., Thauer, R. K., et al. (2016) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1600298113 · Cited by 339 · see citing works
“At the low concentrations of 3-NOP and of nitrite required to completely inhibit methanogenesis, these compounds are not toxic to animals (19, 35, 36).”
BackgroundInfluentialInvited review: current enteric methane mitigation options
Beauchemin, K. A., Ungerfeld, E. M., Abdalla, A. L., Alvarez, C., Arndt, C., Becquet, P., Benchaar, C., Berndt, A., Mauricio, R. M., McAllister, T. A., et al. (2022) · Journal of Dairy Science · doi:10.3168/jds.2022-22091 · Cited by 324 · see citing works
“Long-term in vivo inhibition of enteric CH4 production by 3-NOP was initially reported by Hristov et al. (2015) and has since been confirmed in various studies (Yu et al., 2021).”
Danielsson, R., Dicksved, J., Sun, L., Gonda, H. L., Müller, B., Schnürer, A., & Bertilsson, J. (2017) · Frontiers in Microbiology · doi:10.3389/fmicb.2017.00226 · Cited by 317 · see citing works
“However, recently, the use of 3-nitrooxypropanol, a compound designed to inhibit the activity of the enzyme responsible for formation of CH4 (Duval and Kindermann, 2012), was shown to reduce CH4 emissions in dairy cows without any signs of toxic effects on the animal and no or a minor effect on DMI (Reynolds et al., 2…”
BackgroundDiverse hydrogen production and consumption pathways influence methane production in ruminants
Greening, C., Geier, R. R., Wang, C., Woods, L. C., Morales, S. E., McDonald, M. J., Rushton-Green, R., Morgan, X. C., Koike, S., Leahy, S. C., et al. (2019) · The ISME Journal · doi:10.1038/s41396-019-0464-2 · Cited by 296 · see citing works
The Role of the Gut Microbiome in Cattle Production and Health: Driver or Passenger?
O’Hara, E., Neves, A. L. A., Song, Y., & Guan, L. L. (2020) · Annual Review of Animal Biosciences · doi:10.1146/annurev-animal-021419-083952 · Cited by 251 · see citing works
“Supplementation of 3-NOP has been shown to dramatically reduce ruminal CH4 production in lactating dairy cows and crucially does not have any adverse effect on milk yield (32), though an increase in milk fat has been reported (86).”
BackgroundAgriculture, Forestry and Other Land Uses (AFOLU)
Change, I. P. O. C. (2023) · Cambridge University Press eBooks · doi:10.1017/9781009157926.009 · Cited by 287 · see citing works
Honan, M. C., Feng, X., Tricárico, J. M., & Kebreab, E. (2021) · Animal Production Science · doi:10.1071/an20295 · Cited by 234 · see citing works
“In a meta-analysis of the anti-methanogenic effects of 3NOP, Dijkstra et al. (2018) reported that enteric CH4 production was reduced 39% in dairy and 22% in beef cattle at a mean dose of 0.123 g/kg DM. Additive dose and the neutral detergent fibre (NDF) content of diet had a significant impact on the effectiveness of…”
BackgroundInfluentialGlasson, C. R. K., Kinley, R. D., de Nys, R., King, N., Adams, S. L., Packer, M. A., Svenson, J., Eason, C., & Magnusson, M. E. (2022) · Algal Research · doi:10.1016/j.algal.2022.102673 · Cited by 222 · see citing works
Llonch, P., Haskell, M. J., Dewhurst, R. J., & Turner, S. P. (2016) · animal · doi:10.1017/s1751731116001440 · Cited by 207 · see citing works
“recent study (Hristov et al., 2015) extended the trial to 14 weeks, achieving an average 30% CH4 reduction, and no toxic effects were observed.”
BackgroundThauer, R. K. (2019) · Biochemistry · doi:10.1021/acs.biochem.9b00164 · Cited by 210 · see citing works
Hammond, K. J., Crompton, L. A., Bannink, A., Dijkstra, J., Yáñez-Ruíz, D. R., O’Kiely, P., Kebreab, E., Eugène, M., Yu, Z., Shingfield, K. J., et al. (2016) · Animal Feed Science and Technology · doi:10.1016/j.anifeedsci.2016.05.018 · Cited by 202 · see citing works
“digestive and metabolic activity at a fixed level of feed intake can affect CO2 emission, as well as variation in rumen fermentation, and thereby change the CH4:CO2 ratio, the estimated CO2 emission and thus predicted CH4 emission (Huhtanen et al., 2015).”
BackgroundInfluentialStefenoni, H., Räisänen, S. E., Cueva, S. F., Wasson, D. E., Lage, C. F. A., Audino Melgar, M., Fetter, M. E., Smith, P. B. W., Hennessy, M. L., Vecchiarelli, B., et al. (2021) · Journal of Dairy Science · doi:10.3168/jds.2020-19686 · Cited by 206 · see citing works
Physiology, Biochemistry, and Applications of F 420 - and F o -Dependent Redox Reactions
Greening, C., Ahmed, F. H., Mohamed, A. E., Lee, B. M., Pandey, G., Warden, A. C., Scott, C. S., Oakeshott, J. G., Taylor, M. C., & Jackson, C. J. (2016) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.00070-15 · Cited by 188 · see citing works
Loboguerrero, A. M., Campbell, B., Cooper, P. J. M., Hansen, J., Rosenstock, T. S., & Wollenberg, E. (2019) · Sustainability · doi:10.3390/su11051372 · Cited by 205 · see citing works
Hristov, A. N., Kebreab, E., Niu, M., Oh, J., Bannink, A., Bayat, A. R., Boland, T. M., Brito, A. F., Casper, D. P., Crompton, L. A., et al. (2018) · Journal of Dairy Science · doi:10.3168/jds.2017-13536 · Cited by 198 · see citing works
“, 2016) and 3-nitrooxypropanol (Hristov et al., 2015b) in existing empirical models.”
Dijkstra, J., Bannink, A., France, J., Kebreab, E., & van Gastelen, S. (2018) · Journal of Dairy Science · doi:10.3168/jds.2018-14456 · Cited by 180 · see citing works
Could propionate formation be used to reduce enteric methane emission in ruminants?
Wang, K., Xiong, B., & Zhao, X. (2022) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2022.158867 · Cited by 178 · see citing works
How much do direct livestock emissions actually contribute to global warming?
Reisinger, A., & Clark, H. (2018) · Global Change Biology · doi:10.1111/gcb.13975 · Cited by 197 · see citing works
“…demandside management (e.g. Aleksandrowicz et al., 2016; Alexander et al., 2015; Stehfest et al., 2009) or novel supply side mitigation technologies, such as inhibitors that suppress enteric CH4 (Hristov et al., 2015) or N2O from pastoral soils (Di & Cameron, 2002; Misselbrook et al., 2014).”
BackgroundChange, I. P. O. C. (2022) · Cambridge University Press eBooks · doi:10.1017/9781009157988.007 · Cited by 193 · see citing works
Kebreab, E., Bannink, A., Pressman, E. M., Walker, N. D., Karagiannis, A., van Gastelen, S., & Dijkstra, J. (2022) · Journal of Dairy Science · doi:10.3168/jds.2022-22211 · Cited by 163 · see citing works
“Data from 13 articles (14 experiments) met the selection criteria, and 48 treatment means were used for dairy cattle (Haisan et al., 2014, 2017; Hristov et al., 2015; Lopes et al., 2016; Van Wesemael et al., 2019; Melgar et al., 2020a,b, 2021; van Gastelen et al., 2020, 2022; Yanibada et al., 2020; Bampidis et al., 20…”
MethodMin, B. R., Solaiman, S., Waldrip, H. M., Parker, D., Todd, R. W., & Brauer, D. (2020) · Animal nutrition · doi:10.1016/j.aninu.2020.05.002 · Cited by 159 · see citing works
Symposium review: Effective nutritional strategies to mitigate enteric methane in dairy cattle
Hristov, A. N., Audino Melgar, M., Wasson, D. E., & Arndt, C. (2022) · Journal of Dairy Science · doi:10.3168/jds.2021-21398 · Cited by 159 · see citing works
Jayanegara, A., Sarwono, K. A., Kondo, M., Matsui, H., Ridla, M., Laconi, E. B., & Nahrowi (2018) · Italian Journal of Animal Science · doi:10.1080/1828051x.2017.1404945 · Cited by 158 · see citing works
Review: Selecting for improved feed efficiency and reduced methane emissions in dairy cattle
Løvendahl, P., Difford, G. F., Li, B., Chagunda, M. G. G., Huhtanen, P. J., Lidauer, M., Lassen, J., & Lund, P. (2018) · animal · doi:10.1017/s1751731118002276 · Cited by 157 · see citing works
“There are exceptions, including nitrate and the patented compound 3-nitrooxypropanol, a methyl coenzyme M reductase inhibitor, which decreases CH4 emissions by up to 30% in lactating cows without compromising feed intake or milk production (Hristov et al., 2015).”
BackgroundMitigation Pathways Compatible with 1.5°C in the Context of Sustainable Development
IPCC (2022) · Cambridge University Press eBooks · doi:10.1017/9781009157940.004 · Cited by 167 · see citing works
Martı́nez, G., Denman, S. E., Yang, C., Cheung, J., Mitsumori, M., & McSweeney, C. S. (2016) · Frontiers in Microbiology · doi:10.3389/fmicb.2016.01122 · Cited by 150 · see citing works
“The observed 30% decrease in methanogenesis did not adversely affect rumen metabolism and the rumen microbiota was able to adapt and redirect [H] into other microbial end-products for both diets.”
BackgroundLopes, J. C., de Matos, L. F., Harper, M. T., Giallongo, F., Oh, J., Gruen, D. S., Ono, S., Kindermann, M., Duval, S. M., & Hristov, A. N. (2016) · Journal of Dairy Science · doi:10.3168/jds.2015-10832 · Cited by 147 · see citing works
Key Considerations for the Use of Seaweed to Reduce Enteric Methane Emissions From Cattle
Vijn, S., Compart, D. P., Dutta, N., Foukis, A., Hess, M., Hristov, A. N., Kalscheur, K. F., Kebreab, E., Nuzhdin, S. V., Price, N. N., et al. (2020) · Frontiers in Veterinary Science · doi:10.3389/fvets.2020.597430 · Cited by 144 · see citing works
“Feed additives, such as the inhibitor 3-nitrooxypropanol (3-NOP), have been shown to consistently decrease enteric methane emissions by up to 30% in both dairy and beef cattle (7, 8).”
BackgroundAchieving net-zero emissions in agriculture: a review
Rosa, L., & Gabrielli, P. (2023) · Environmental Research Letters · doi:10.1088/1748-9326/acd5e8 · Cited by 154 · see citing works
“Moreover, some inhibitors and feed additivesmay have unforeseen environmental implications, and public acceptance issues could greatly impede their widespread implementation [47].”
BackgroundHristov, A. N., Oh, J., Giallongo, F., Frederick, T. W., Weeks, H. L., ZIMMERMAN, P. R., Harper, M. T., Hristova, R. A., Zimmerman, R. S., & Branco, A. F. (2015) · Journal of Visualized Experiments · doi:10.3791/52904 · Cited by 138 · see citing works
Reisinger, A. R., Clark, H., Cowie, A., Emmet‐Booth, J., Fischer, C. G., Herrero, M., Howden, M. S., & Leahy, S. C. (2021) · Philosophical Transactions of the Royal Society A Mathematical Physical and Engineering Sciences · doi:10.1098/rsta.2020.0452 · Cited by 143 · see citing works
“3-Nitrooxypropanol (3-NOP) has been shown to consistently reduce CH4 emissions by around 30% in Total Mixed Ration (TMR) farm systems [66,67] without compromising animal productivity [68] and is expected to be commercially available in some countries within the next 2 years.”
BackgroundBlack, J. L., Davison, T. M., & Box, I. (2021) · Animals · doi:10.3390/ani11040951 · Cited by 137 · see citing works
“The potential role of vaccination against rumen Archaea, including peptide sequences, the commercially developed 3-nitrooxypropanol (3-NOP) [19] and biochar were not included in NLMP research, but were evaluated.”
BackgroundNutritional strategies in ruminants: A lifetime approach
McGrath, J. J., Duval, S. M., Tamassia, L. F. M., Kindermann, M., Stemmler, R. T., Gouvêa, V. N., Acedo, T. S., Immig, I., Williams, S. N., & Celi, P. (2017) · Research in Veterinary Science · doi:10.1016/j.rvsc.2017.09.011 · Cited by 142 · see citing works
Audino Melgar, M., Harper, M. T., Oh, J., Giallongo, F., Young, M.-P., OTT, T. L., Duval, S. M., & Hristov, A. N. (2019) · Journal of Dairy Science · doi:10.3168/jds.2019-17085 · Cited by 131 · see citing works
“Procedures were exempted from Institutional Review Board and reviewed by professional staff in The Pennsylvania State University Office of Research Protections [exemption in 45 CFR 46.101(b) (6) for Food Taste and Acceptance Studies].”
BackgroundMethodResultInfluentialProspects of artificial meat: Opportunities and challenges around consumer acceptance
Zhang, L., Hu, Y., Badar, I. H., Xia, X., Kong, B., & Chen, Q. (2021) · Trends in Food Science & Technology · doi:10.1016/j.tifs.2021.07.010 · Cited by 133 · see citing works
Leahy, S. C., Clark, H., & Reisinger, A. R. (2020) · Frontiers in Sustainable Food Systems · doi:10.3389/fsufs.2020.00069 · Cited by 129 · see citing works
“Most of the development of a methane inhibitor is being carried out in developed countries, with the most advanced product (3NOP) so far showing high efficacy (>30% reduction) in feedlot systems (Hristov et al., 2015) but limited suitability in grazing based systems (Reisinger et al.”
BackgroundRecent Advances in Measurement and Dietary Mitigation of Enteric Methane Emissions in Ruminants
Patra, A. K. (2016) · Frontiers in Veterinary Science · doi:10.3389/fvets.2016.00039 · Cited by 126 · see citing works
“Feeding of NOP at 40–80 mg/kg diet in dairy cattle was also associated with decreased CH4 production by 30% (126).”
BackgroundAlmeida, A. K., Hegarty, R. S., & Cowie, A. (2021) · Animal nutrition · doi:10.1016/j.aninu.2021.09.005 · Cited by 128 · see citing works
Insights on Alterations to the Rumen Ecosystem by Nitrate and Nitrocompounds
Latham, E. A., Anderson, R. C., Pinchak, W. E., & Nisbet, D. J. (2016) · Frontiers in Microbiology · doi:10.3389/fmicb.2016.00228 · Cited by 121 · see citing works
“…ruminal methane emissions from dairy or beef cattle and results have yielded positive results, with methane emissions, based on dry matter intake, being decreased from controls by 6 to nearly 60% (Haisan et al., 2014; Reynolds et al., 2014; Romero-Perez et al., 2014, 2015b; Hristov et al., 2015).”
BackgroundA 100-Year Review: A century of change in temperate grazing dairy systems
Roche, J. R., Berry, D. P., Bryant, A. M., Burke, C. R., Butler, S. T., Dillon, P., Donaghy, D. J., Horan, B., Macdonald, K. A., & Macmillan, K. L. (2017) · Journal of Dairy Science · doi:10.3168/jds.2017-13182 · Cited by 121 · see citing works
“Although CH4 inhibitors are being explored (Hristov et al., 2015), currently they need to be included with the feed and may not be effective in grazing systems.”
BackgroundVyas, D., Alemu, A. W., McGinn, S. M., Duval, S. M., Kindermann, M., & Beauchemin, K. A. (2018) · Journal of Animal Science · doi:10.1093/jas/sky174 · Cited by 120 · see citing works
“However, only 31% (5 g/d) of expected H2 was released; thus, it appears that the remaining spared H2 was diverted toward alternate H2 sinks or toward dissolved H2, which is a larger pool compared with gaseous H2 emissions (Janssen, 2010).”
BackgroundYang, C., Rooke, J. A., Cabeza, I., & Wallace, R. J. (2016) · Frontiers in Microbiology · doi:10.3389/fmicb.2016.00132 · Cited by 121 · see citing works
“An overall benefit, due to mitigation of methanogenesis by dietary nitrate supplementation, on ruminant animal energetics has not been detected (van Zijderveld et al., 2011a; Knapp et al., 2014; Hristov et al., 2015).”
BackgroundDose-response effect of 3-nitrooxypropanol on enteric methane emissions in dairy cows
Melgar, A., Welter, K. C., Nedelkov, K., Martins, C. M. M. R., Harper, M. T., Oh, J., Räisänen, S. E., Chen, X., Cueva, S. F., Duval, S., et al. (2020) · Journal of Dairy Science · doi:10.3168/jds.2019-17840 · Cited by 112 · see citing works
“Dose-response effects of 3-NOP have been studied in beef cattle (Romero-Pérez et al., 2014; Vyas et al., 2016) and dairy cows (Reynolds et al., 2014; Hristov et al., 2015a).”
InfluentialSelf-citationvan Gastelen, S., Dijkstra, J., & Bannink, A. (2019) · Journal of Dairy Science · doi:10.3168/jds.2018-15785 · Cited by 111 · see citing works
Manafiazar, G., Zimmerman, S., & Basarab, J. A. (2016) · Canadian Journal of Animal Science · doi:10.1139/cjas-2015-0190 · Cited by 110 · see citing works
Homo-Acetogens: Their Metabolism and Competitive Relationship with Hydrogenotrophic Methanogens
Karekar, S. C., Stefanini, R., & Ahring, B. K. (2022) · Microorganisms · doi:10.3390/microorganisms10020397 · Cited by 101 · see citing works
Martı́nez, G., Duval, S. M., Kindermann, M., Schirra, H. J., Denman, S. E., & McSweeney, C. S. (2018) · Frontiers in Microbiology · doi:10.3389/fmicb.2018.01582 · Cited by 102 · see citing works
Callaway, T. R., Lillehoj, H. S., Chuanchuen, R., & Gay, C. G. (2021) · Antibiotics · doi:10.3390/antibiotics10050471 · Cited by 100 · see citing works
“, probiotic and prebiotic feed additives [29], 3-nitrooxypropanol (3-NOP) [30], bacteriophage [31], management practices, dietary changes, organic acid inclusion, and vaccination.”
BackgroundClimate Change Impact, Adaptation, and Mitigation in Temperate Grazing Systems: A Review
Ghahramani, A., Howden, M. S., del Prado, A., Thomas, D., Moore, A. D., Ji, B., & Ateş, S. (2019) · Sustainability · doi:10.3390/su11247224 · Cited by 99 · see citing works
“These include provision of additional dietary oils [154], ionophores and other additives [19] including 3-nitrooxypropanol (3NOP) [155], nitrates [156], tannins and saponins [157], specific shrubs and pasture species with anti-methanogenic properties [154,157], higher digestibility forages and feedstuffs such as red-a…”
BackgroundInhibition of Rumen Methanogenesis and Ruminant Productivity: A Meta-Analysis
Ungerfeld, E. M. (2018) · Frontiers in Veterinary Science · doi:10.3389/fvets.2018.00113 · Cited by 97 · see citing works
“Importantly, in some experiments in which the energy gain obtained from inhibiting CH4 production surpassed the energy losses as H2 there was still no increase in productivity associated (25, 26, 56, 60, 62).”
BackgroundReview: Optimizing ruminant conversion of feed protein to human food protein
Broderick, G. A. (2017) · animal · doi:10.1017/s1751731117002592 · Cited by 96 · see citing works
“Recent reports indicate that the inhibitor 3-nitrooxypropanol reduced methane production by more than 30%, an effect that persisted for at least 12 weeks (Hristov et al., 2015).”
The social cost of methane: theory and applications
Shindell, D. T., Fuglestvedt, J. S., & Collins, W. J. (2017) · Faraday Discussions · doi:10.1039/c7fd00009j · Cited by 96 · see citing works
Reducing enteric methane emissions from dairy cattle: Two ways to supplement 3-nitrooxypropanol
Wesemael, D. V., Vandaele, L., Ampe, B., Cattrysse, H., Duval, S. M., Kindermann, M., Fievez, V., Campeneere, S. D., & Peiren, N. (2018) · Journal of Dairy Science · doi:10.3168/jds.2018-14534 · Cited by 92 · see citing works
Enteric methane emission, milk production, and composition of dairy cows fed 3-nitrooxypropanol
Audino Melgar, M., Lage, C. F. A., Nedelkov, K., Räisänen, S. E., Stefenoni, H., Fetter, M. E., Chen, X., Oh, J., Duval, S. M., Kindermann, M., et al. (2020) · Journal of Dairy Science · doi:10.3168/jds.2020-18908 · Cited by 95 · see citing works
Mapping and linking supply- and demand-side measures in climate-smart agriculture. A review
Scherer, L., & Verburg, P. H. (2017) · Agronomy for Sustainable Development · doi:10.1007/s13593-017-0475-1 · Cited by 95 · see citing works
“Examples of feed optimization include diets richer in concentrates (Ross et al. 2014) and supplementation with methane inhibitors (Hristov et al. 2015).”
BackgroundMethodInfluentialA Review of 3-Nitrooxypropanol for Enteric Methane Mitigation from Ruminant Livestock
Yu, G., Beauchemin, K. A., & Dong, R. (2021) · Animals · doi:10.3390/ani11123540 · Cited by 89 · see citing works
Vyas, D., McGinn, S. M., Duval, S. M., Kindermann, M., & Beauchemin, K. A. (2016) · Journal of Animal Science · doi:10.2527/jas.2015-0268 · Cited by 89 · see citing works
Ugbogu, E. A., Elghandour, M. M. M. Y., Ikpeazu, V. O., Rodríguez, G. B., Molina, O. M., Arunsi, U. O., Emmanuel, O., & Salem, A. Z. M. (2018) · Journal of Cleaner Production · doi:10.1016/j.jclepro.2018.12.233 · Cited by 91 · see citing works
van Gastelen, S., Dijkstra, J., Binnendijk, G. P., Duval, S. M., Heck, J. M. L., Kindermann, M., Zandstra, T., & Bannink, A. (2020) · Journal of Dairy Science · doi:10.3168/jds.2019-17936 · Cited by 84 · see citing works
The GreenFeed system for measurement of enteric methane emission from cattle
Hammond, K. J., Waghorn, G. C., & Hegarty, R. S. (2016) · Animal Production Science · doi:10.1071/an15631 · Cited by 83 · see citing works
van Gastelen, S., Dijkstra, J., Heck, J. M. L., Kindermann, M., Klop, A., de Mol, R. M., Rijnders, D., Walker, N. D., & Bannink, A. (2022) · Journal of Dairy Science · doi:10.3168/jds.2021-20782 · Cited by 83 · see citing works
Pitta, D., Indugu, N., Audino Melgar, M., Hristov, A. N., Challa, K., Vecchiarelli, B., Hennessy, M. L., Narayan, K. S., Duval, S. M., Kindermann, M., et al. (2022) · Microbiome · doi:10.1186/s40168-022-01341-9 · Cited by 83 · see citing works
“Further, these authors observed that H2 emissions increased under inhibited methanogenesis for the first 8 weeks and then steadily declined in 3-NOP supplemented cows over the remainder of the 15-week period [7].”
BackgroundMeale, S. J., Popova, M., Saro, C., Martin, C., Bernard, A., Lagrée, M., Yáñez-Ruíz, D. R., Boudra, H., Duval, S. M., & Morgavi, D. P. (2021) · Scientific Reports · doi:10.1038/s41598-021-82084-9 · Cited by 78 · see citing works
Kim, H., Lee, H. G., Baek, Y.-C., Lee, S., & Seo, J. (2020) · Journal of Animal Science and Technology · doi:10.5187/jast.2020.62.1.31 · Cited by 77 · see citing works
“With our knowledge, total 5 in vivo studies were conducted to investigate the effects of NOP supplementation on sustained mitigation of CH4 emission [15,16,19,22,25].”
BackgroundInfluentialCofactor F420: an expanded view of its distribution, biosynthesis and roles in bacteria and archaea
Grinter, R., & Greening, C. (2021) · FEMS Microbiology Reviews · doi:10.1093/femsre/fuab021 · Cited by 73 · see citing works
Vyas, D., McGinn, S. M., Duval, S. M., Kindermann, M., & Beauchemin, K. A. (2016) · Animal Production Science · doi:10.1071/an15705 · Cited by 75 · see citing works
Pitta, D., Indugu, N., Narayan, K. S., & Hennessy, M. L. (2022) · Journal of Dairy Science · doi:10.3168/jds.2021-21466 · Cited by 76 · see citing works
Inclusion of wheat and triticale silage in the diet of lactating dairy cows
Harper, M. T., Oh, J., Giallongo, F., Roth, G. W., & Hristov, A. N. (2017) · Journal of Dairy Science · doi:10.3168/jds.2017-12553 · Cited by 72 · see citing works
Montoya-Flores, M. D., Molina-Botero, I. C., Arango, J., Romano-Muñoz, J. L., Solorio‐Sánchez, F. J., Aguilar-Pérez, C. F., & Kú-Vera, J. C. (2020) · Animals · doi:10.3390/ani10020300 · Cited by 69 · see citing works
“Similar observations have been suggested when evaluating mitigation strategies for extended periods of time and interpreting the results of mitigating GHG in kg of product (milk and meat) obtained [68].”
BackgroundReview: Methanogens and methane production in the digestive systems of nonruminant farm animals
Misiukiewicz, A., Gao, M., Filipiak, W., Cieślak, A., Patra, A. K., & Szumacher‐Strabel, M. (2020) · animal · doi:10.1016/j.animal.2020.100060 · Cited by 68 · see citing works
Enteric methane mitigation interventions
Fouts, J. Q., Honan, M. C., Roque, B. M., Tricárico, J. M., & Kebreab, E. (2022) · Translational Animal Science · doi:10.1093/tas/txac041 · Cited by 68 · see citing works
Zhang, X. M., Smith, M. L., Gruninger, R. J., Kung, L., Vyas, D., McGinn, S. M., Kindermann, M., Wang, M., Tan, Z. L., & Beauchemin, K. A. (2021) · Journal of Animal Science · doi:10.1093/jas/skab081 · Cited by 65 · see citing works
Energy and Climate Footprint Towards the Environmental Sustainability
Banerjee, A., Jhariya, M. K., Raj, A., Yadav, D. K., Khan, N. A., & Meena, R. S. (2020) · Springer eBooks · doi:10.1007/978-981-15-9496-0_14 · Cited by 67 · see citing works
Belanche, A., Yáñez-Ruíz, D. R., Detheridge, A. P., Griffith, G. W., Kingston‐Smith, A. H., & Newbold, C. J. (2019) · Environmental Microbiology · doi:10.1111/1462-2920.14801 · Cited by 65 · see citing works
“, 2010) and there has been significant research effort targeting this microbial community in order to decrease methane emissions (Hristov et al., 2015).”
BackgroundWallace, R. J., Snelling, T. J., McCartney, C. A., Tapio, I., & Strozzi, F. (2017) · Genetics Selection Evolution · doi:10.1186/s12711-017-0285-6 · Cited by 63 · see citing works
“However, unfortunately that largely intuitive prediction does not seem to hold in practice [22, 23], thus weakening the incentive to farmers to adopt measures that would lower methane emissions.”
BackgroundSustainability of the Dairy Industry: Emissions and Mitigation Opportunities
Peterson, C. B., & Mitloehner, F. M. (2021) · Frontiers in Animal Science · doi:10.3389/fanim.2021.760310 · Cited by 65 · see citing works
“, 2016), as well as Holstein dairy cattle (Reynolds et al., 2014; Hristov et al., 2015; Lopes et al., 2016; Haisan et al., 2017).”
Backgroundvan Gastelen, S., Burgers, E. E. A., Dijkstra, J., de Mol, R. M., Muizelaar, W., Walker, N. D., & Bannink, A. (2024) · Journal of Dairy Science · doi:10.3168/jds.2023-24198 · Cited by 62 · see citing works
Weishaupt, A., Ekardt, F., Garske, B., Stubenrauch, J., & Wieding, J. (2020) · Sustainability · doi:10.3390/su12052053 · Cited by 62 · see citing works
Management of Enteric Methanogenesis in Ruminants by Algal-Derived Feed Additives
McCauley, J. I., Labeeuw, L., Jaramillo-Madrid, A. C., Nguyen, L. N., Nghiem, L. D., Chaves, A. V., & Ralph, P. J. (2020) · Current Pollution Reports · doi:10.1007/s40726-020-00151-7 · Cited by 65 · see citing works
Krizsan, S. J., Ramin, M., Chagas, J. C. C., Halmemies‐Beauchet‐Filleau, A., Singh, A., Schnürer, A., & Danielsson, R. (2023) · Frontiers in Animal Science · doi:10.3389/fanim.2023.1112969 · Cited by 60 · see citing works
“The inhibitor 3-nitroxypropanol has demonstrated CH4 reductions of only approximately 30%, when given in very low doses to dairy cows (Hristov et al., 2015).”
BackgroundRomero, P., Belanche, A., Jiménez, E., Hueso, R., Ramos‐Morales, E., Salwen, J. K., Kebreab, E., & Yáñez-Ruíz, D. R. (2023) · Journal of Animal Science and Biotechnology/Journal of animal science and biotechnology · doi:10.1186/s40104-023-00935-z · Cited by 60 · see citing works
“The substantial reduction of CH 4 production by the addition of AT was accompanied by an increase in rumen H 2 accumulation, in agreement with previous studies that used AT [8, 9] and other anti-methanogenic feed additives [51, 52].”
ResultMoate, P. J., Deighton, M. H., Williams, S. R. O., Pryce, J. E., Hayes, B. J., Jacobs, J. L., Eckard, R., Hannah, M. C., & Wales, W. J. (2015) · Animal Production Science · doi:10.1071/an15222 · Cited by 60 · see citing works
“Recent research with dairy cows in Canada and the USA has shown that administration of NOP was associated with reduced methane production (Haisan et al. 2014; Hristov et al. 2015).”
BackgroundMartinez-Fernandez, G., Denman, S. E., Cheung, J., & McSweeney, C. S. (2017) · Frontiers in Microbiology · doi:10.3389/fmicb.2017.01871 · Cited by 59 · see citing works
“A recent trial with dairy cattle using 3-nitrooxypropanol to reduce CH4 emissions produced an 80% increase on the average body weight gain over the experimental period (Hristov et al., 2015).”
BackgroundUngerfeld, E. M. (2015) · Frontiers in Microbiology · doi:10.3389/fmicb.2015.01272 · Cited by 58 · see citing works
In Vitro Evaluation of Different Dietary Methane Mitigation Strategies
Chagas, J. C. C., Ramin, M., & Krizsan, S. J. (2019) · Animals · doi:10.3390/ani9121120 · Cited by 58 · see citing works
“The most successful inhibitor suggested in vivo so far is 3-nitroxypropanol that has showed CH4 reducing effects when provided to dairy cows in a low dose [12].”
Background
- Authors
- Hristov, A. N., Oh, J., Giallongo, F., Frederick, T. W., Harper, M. T., Weeks, H. L., Branco, A. F., Moate, P. J., Deighton, M. H., Williams, S. R. O., Kindermann, M., & Duval, S.
- Affiliations
- Pennsylvania State University (US)
- Departamento de Zootecnia, Universidade Estadual de Maringá, PR 87020-900, Brazil;
- Department of Economic Development Jobs Transport and Resources (AU)
- Animal Nutrition and Health, DSM Nutritional Products, Basel CH-4002, Switzerland;
- Research Centre for Animal Nutrition and Health, DSM Nutritional Products France, Saint Louis Cedex 68305, France
- ORCID
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2015-07-30
- Volume
- 112
- Issue
- 34
- Pages
- 10663-10668
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC4553761
- OpenAlex
- W2145651012
- Cited by
- 496
- References
- 59
- Citations by year
- 2026: 54 · 2025: 49 · 2024: 58 · 2023: 48 · 2022: 48 · 2021: 53 · 2020: 40 · 2019: 42 · 2018: 35 · 2017: 31 · 2016: 34 · 2015: 4
- FWCI
- 74.95
- Type
- journal article
- Language
- en
- Update notices
- correction (2015-08-12)
- Open access
- bronze
- doi.org
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Seoul National University Open Repository (Seoul National University) (submittedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Ruminant Nutrition and Digestive Physiology
- Physical Sciences › Environmental Science › Ecology › Agriculture Sustainability and Environmental Impact
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
- Keywords
- enteric methane, 3-nitrooxypropanol, methane inhibitors, dairy cattle, Holstein cows, methane mitigation, milk production, milk protein, lactose yield, weight gain, ruminant methane emissions, feed intake, fiber digestibility
- MeSH terms
- Medicago sativa, Animals, Animal Feed, Archaea, Energy Intake, Carbon Dioxide, Cattle, Zea mays, Female, Fermentation, Gases, Hydrogen, Lactation, Methane, Milk, Rumen, Weight Gain, Greenhouse Effect, Dietary Supplements, Propanols
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