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Cited by 18Berberine and alkaloids researchBirth, Development, and HealthGestational Diabetes Research and Management

Supplemental Berberine in a High-Fat Diet Reduces Adiposity and Cardiac Dysfunction in Offspring of Mouse Dams with Gestational Diabetes Mellitus

Open access
Cole, Laura K, Zhang, Ming, Chen, Li, Sparagna, Genevieve C, Vandel, Marilyne, Xiang, Bo, Dolinsky, Vernon W, Hatch, Grant M·2021·The Journal of Nutrition
DOI 10.1093/jn/nxaa408

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Abstract

Background There are few evidence-based strategies to attenuate the risk of metabolic syndrome in offspring exposed to gestational diabetes mellitus (GDM). Berberine (BBR) is an isoquinoline alkaloid extracted from Chinese herbs and exhibits glucose lowering properties. Objectives We hypothesized that dietary BBR would improve health outcomes in the mouse offspring of GDM dams. Methods Wild-type C57BL/6 female mice were fed either a Lean-inducing low-fat diet (L-LF,10% kcal fat, 35% kcal sucrose) or a GDM-inducing high-fat diet (GDM-HF, 45% kcal fat, 17.5% sucrose) for 6 wk prior to breeding with wild-type C57BL/6 male mice throughout pregnancy and the suckling period. The resulting Lean and GDM-exposed male and female offspring were randomly assigned an LF (10% kcal fat, 35% kcal sucrose), HF (45% kcal fat, 17.5% sucrose), or high-fat berberine (HFB) (45% kcal fat, 17.5% sucrose diet) containing BBR (160 mg/kg/d, HFB) at weaning for 12 wk. The main outcome was to evaluate the effects of BBR on obesity, pancreatic islet function, and cardiac contractility in GDM-exposed HF-fed offspring. Significance between measurements was determined using a 2 (gestational exposure) × 3 (diet) factorial design by a 2- way ANOVA using Tukey post-hoc analysis. Results In the GDM-HF group, body weights were significantly increased (16%) compared with those in baseline (L-LF) animals (P < 0.05). Compared with the L-LF animals, the GDM-HF group had a reduction in pancreatic insulin glucose-stimulated insulin secretion (74%) and increased cardiac isovolumetric contraction time (IVCT; ∼150%) (P < 0.05). Compared with GDM-HF animals, the GDM-HFB group with the dietary addition of BBR had significantly reduced body weight (16%), increased glucose-stimulated insulin secretion from pancreatic islets (254%), and reduced systolic heart function (46% IVCT) (P < 0.05). Conclusions In a mouse model of GDM, dietary BBR treatment provided protection from obesity and the development of pancreatic islet and cardiac dysfunction.

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  • Maternal administration of natural compounds could also protect offspring from cardiovascular dysfunction through direct effects on the heart or by mitigating GDM-induced obesity or other cardiometabolic conditions.

    “Therefore, maternal administration of natural compounds could also protect offspring from cardiovascular dysfunction through direct effects on the heart or by mitigating GDM-induced obesity or other cardiometabolic conditions.”

    From Recent Experimental Studies of Maternal Obesity, Diabetes during Pregnancy and the Developmental Origins of Cardiovascular Disease (2022) · cites this paper as [142]

  • Compared to GDM offspring fed a postnatal high-fat-and-sucrose diet, GDM offspring that received berberine in the high-fat-and-sucrose diet had a lower body weight.

    “Compared to GDM offspring fed a postnatal high-fat-and-sucrose diet, the GDM offspring that received berberine in the high-fat-and-sucrose diet had a lower body weight, increased pancreatic islet glucose-stimulated insulin secretion and improved systolic and diastolic heart function [142].”

    From Recent Experimental Studies of Maternal Obesity, Diabetes during Pregnancy and the Developmental Origins of Cardiovascular Disease (2022) · cites this paper as [142]

  • Compared to GDM offspring fed a postnatal high-fat-and-sucrose diet, GDM offspring that received berberine in the high-fat-and-sucrose diet had improved systolic and diastolic heart function.

    “Compared to GDM offspring fed a postnatal high-fat-and-sucrose diet, the GDM offspring that received berberine in the high-fat-and-sucrose diet had a lower body weight, increased pancreatic islet glucose-stimulated insulin secretion and improved systolic and diastolic heart function [142].”

    From Recent Experimental Studies of Maternal Obesity, Diabetes during Pregnancy and the Developmental Origins of Cardiovascular Disease (2022) · cites this paper as [142]

  • Compared to GDM offspring fed a postnatal high-fat-and-sucrose diet, GDM offspring that received berberine in the high-fat-and-sucrose diet had increased pancreatic islet glucose-stimulated insulin secretion.

    “Compared to GDM offspring fed a postnatal high-fat-and-sucrose diet, the GDM offspring that received berberine in the high-fat-and-sucrose diet had a lower body weight, increased pancreatic islet glucose-stimulated insulin secretion and improved systolic and diastolic heart function [142].”

    From Recent Experimental Studies of Maternal Obesity, Diabetes during Pregnancy and the Developmental Origins of Cardiovascular Disease (2022) · cites this paper as [142]

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Authors
Cole, L. K., Zhang, M., Chen, L., Sparagna, G. C., Vandel, M., Xiang, B., Dolinsky, V. W., & Hatch, G. M.
Affiliations
  • Children's Hospital Research Institute of Manitoba (CA)
  • Jilin University (CN)
  • University of Colorado Anschutz (US)
Corresponding author
Hatch, Grant M
ORCID
Venue
The Journal of Nutrition
Publisher
Elsevier BV
Published
2021-04
Volume
151
Issue
4
Pages
892-901
ISSN
0022-3166 (ISSN-L)
DOI
10.1093/jn/nxaa408
OpenAlex
W3122503354
Cited by
18
References
45
Citations by year
2026: 2 · 2025: 5 · 2024: 5 · 2023: 2 · 2022: 3 · 2021: 1
Type
supplementary materials
Language
en
Open access
Licence
Locations
  • Journal of Nutrition (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Heart and Stroke Foundation of Canada (144626)
  • Canada Foundation for Innovation
  • Canada Research Chairs
  • Manitoba Medical Services Foundation
Topics
  • Health Sciences › Medicine › Pharmacology › Berberine and alkaloids research
  • Health Sciences › Medicine › Pediatrics, Perinatology and Child Health › Birth, Development, and Health
  • Health Sciences › Medicine › Obstetrics and Gynecology › Gestational Diabetes Research and Management
Keywords
berberine, gestational diabetes mellitus, high-fat diet, offspring metabolic programming, obesity, cardiac dysfunction, glucose-stimulated insulin secretion, pancreatic islets, isovolumic contraction time, metabolic syndrome, dietary supplementation, isoflavones, isoflavone glycosides, isoflavone glucosides
MeSH terms
Animals, Berberine, Body Weight, Female, Glucose, Heart Diseases, Insulin, Islets of Langerhans, Male, Mice, Inbred C57BL, Mitochondria, Heart, Obesity, Pregnancy, Prenatal Exposure Delayed Effects, Diabetes, Gestational, Dietary Supplements, Adiposity, Mice, Diet, High-Fat

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