Cited by 1,054Berberine and alkaloids researchMetabolism, Diabetes, and CancerNatural Antidiabetic Agents Studies

Berberine, a Natural Plant Product, Activates AMP-Activated Protein Kinase With Beneficial Metabolic Effects in Diabetic and Insulin-Resistant States

Lee, Yun S., Kim, Woo S., Kim, Kang H., Yoon, Myung J., Cho, Hye J., Shen, Yun, Ye, Ji-Ming, Lee, Chul H. +7 more·2006·Diabetes

Abstract

Berberine has been shown to have antidiabetic properties, although its mode of action is not known. Here, we have investigated the metabolic effects of berberine in two animal models of insulin resistance and in insulin-responsive cell lines. Berberine reduced body weight and caused a significant improvement in glucose tolerance without altering food intake in db/db mice. Similarly, berberine reduced body weight and plasma triglycerides and improved insulin action in high-fat–fed Wistar rats. Berberine downregulated the expression of genes involved in lipogenesis and upregulated those involved in energy expenditure in adipose tissue and muscle. Berberine treatment resulted in increased AMP-activated protein kinase (AMPK) activity in 3T3-L1 adipocytes and L6 myotubes, increased GLUT4 translocation in L6 cells in a phosphatidylinositol 3′ kinase–independent manner, and reduced lipid accumulation in 3T3-L1 adipocytes. These findings suggest that berberine displays beneficial effects in the treatment of diabetes and obesity at least in part via stimulation of AMPK activity.

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Crossref · 10.2337/db06-0006

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55 of 55 references verified0 citation errors · 0 not checkable

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Authors
Lee, Y. S., Kim, W. S., Kim, K. H., Yoon, M. J., Cho, H. J., Shen, Y., Ye, J.-M., Lee, C. H., Oh, W. K., Kim, C. T., Hohnen-Behrens, C., Gosby, A., Kraegen, E. W., James, D. E., & Kim, J. B.
Affiliations
  • Seoul National University (KR)
  • Garvan Institute of Medical Research (AU)
  • Korea Research Institute of Bioscience and Biotechnology (KR)
Venue
Diabetes
Published
2006-08-01
Volume
55
Issue
8
Pages
2256-2264
ISSN
  • 0012-1797 (ISSN-L)
  • 1939-327X
OpenAlex
W2117680364
Cited by
1,054
References
55
Citations by year
2026: 33 · 2025: 35 · 2024: 30 · 2023: 45 · 2022: 59 · 2021: 65 · 2020: 73 · 2019: 62 · 2018: 59 · 2017: 77 · 2016: 62 · 2015: 57 · 2014: 67 · 2013: 66 · 2012: 51
FWCI
20.18
Type
journal article
Language
en
Open access
closed
Locations
  • Diabetes (publishedVersion)
  • PubMed (publishedVersion)
  • RMIT Research Repository (RMIT University Library) (submittedVersion)
  • Figshare (submittedVersion)
  • KRIBB Repository (submittedVersion)
Topics
  • Health Sciences › Medicine › Pharmacology › Berberine and alkaloids research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Natural Antidiabetic Agents Studies
Keywords
berberine, AMPK, insulin resistance, obesity, glucose tolerance, lipogenesis, energy expenditure, GLUT4 translocation, adipocytes, myotubes, triglycerides, db/db mice, high-fat diet, antidiabetic natural products
MeSH terms
Adipose Tissue, Animals, Berberine, Cell Line, Diabetes Mellitus, Diet, Dietary Fats, Energy Metabolism, Enzyme Activation, Gene Expression Regulation, Insulin Resistance, Lipids, Liver, Male, Mice, Inbred C57BL, Mice, Obese, Multienzyme Complexes, Obesity, Phosphorylation, Glucose Clamp Technique, Weight Loss, Rats, Wistar, Protein Serine-Threonine Kinases, Adipocytes, Muscle, Skeletal, Muscle Fibers, Skeletal, Phosphatidylinositol 3-Kinases, 3T3-L1 Cells, Glucose Transporter Type 4, Mice, Rats, AMP-Activated Protein Kinases

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