Leaf Positioning of Arabidopsis in Response to Blue Light
Open accessAbstract
Appropriate leaf positioning is essential for optimizing photosynthesis and plant growth. However, it has not been elucidated how green leaves reach and maintain their position for capturing light. We show here the regulation of leaf positioning under blue light stimuli. When 1-week-old Arabidopsis seedlings grown under white light were transferred to red light (25 micromol m(-2) s(-1)) for 5 d, new petioles that appeared were almost horizontal and their leaves were curled and slanted downward. However, when a weak blue light from above (0.1 micromol m(-2) s(-1)) was superimposed on red light, the new petioles grew obliquely upward and the leaves were flat and horizontal. The leaf positioning required both phototropin1 (phot1) and nonphototropic hypocotyl 3 (NPH3), and resulted in enhanced plant growth. In an nph3 mutant, neither optimal leaf positioning nor leaf flattening by blue light was found, and blue light-induced growth enhancement was drastically reduced. When blue light was increased from 0.1 to 5 micromol m(-2) s(-1), normal leaf positioning and leaf flattening were induced in both phot1 and nph3 mutants, suggesting that phot2 signaling became functional and that the signaling was independent of phot1 and NPH3 in these responses. When plants were irradiated with blue light (0.1 micromol m(-2) s(-1)) from the side and red light from above, the new leaves became oriented toward the source of blue light. When we transferred these plants to both blue light and red light from above, the leaf surface changed its orientation to the new blue light source within a few hours, whereas the petioles initially were unchanged but then gradually rotated, suggesting the plasticity of leaf positioning in response to blue light. We showed the tissue expression of NPH3 and its plasma membrane localization via the coiled-coil domain and the C-terminal region. We conclude that NPH3-mediated phototropin signaling optimizes the efficiency of light perception by inducing both optimal leaf positioning and leaf flattening, and enhances plant growth.
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The nph3 mutant is defective in leaf extension.
“This mutant is also defective in leaf extension”
From The action of enhancing weak light capture via phototropic growth and chloroplast movement in plants (2022) · cites this paper as Inoue et al., 2008a
The nph3 mutant is impaired in photosynthesis and plant growth under weak blue light conditions.
“The nph3 mutant is also impaired in photosynthesis and plant growth under weak BL conditions.”
From The action of enhancing weak light capture via phototropic growth and chloroplast movement in plants (2022) · cites this paper as Inoue et al., 2008a
Cites
phot1 and phot2 mediate blue light regulation of stomatal opening
Kinoshita, T., Doi, M., Suetsugu, N., Kagawa, T., Wada, M., & Shimazaki, K.-I. (2001) · Nature · doi:10.1038/414656a · Cited by 899 · cited 3× · see citing works
“, 2001), stomatal opening (Kinoshita et al., 2001; Doi et al., 2004), and leaf flattening (Sakamoto and Briggs, 2002; Takemiya et al.”
BackgroundSelf-citationPhototropins 1 and 2: versatile plant blue-light receptors
Briggs, W. R., & Christie, J. M. (2002) · Trends in Plant Science · doi:10.1016/s1360-1385(02)02245-8 · Cited by 785 · see citing works
Chloroplast avoidance movement reduces photodamage in plants
Kasahara, M., Kagawa, T., Oikawa, K., Suetsugu, N., Miyao, M., & Wada, M. (2002) · Nature · doi:10.1038/nature01213 · Cited by 581 · see citing works
Cellular and Subcellular Localization of Phototropin 1
Sakamoto, K., & Briggs, W. R. (2002) · The Plant Cell · doi:10.1105/tpc.003293 · Cited by 405 · see citing works
<i>Arabidopsis</i> NPH3: A NPH1 Photoreceptor-Interacting Protein Essential for Phototropism
Motchoulski, A., & Liscum, E. (1999) · Science · doi:10.1126/science.286.5441.961 · Cited by 320 · see citing works
Phototropins Promote Plant Growth in Response to Blue Light in Low Light Environments
Takemiya, A., Inoue, S.-I., Doi, M., Kinoshita, T., & Shimazaki, K.-I. (2005) · The Plant Cell · doi:10.1105/tpc.104.030049 · Cited by 279 · see citing works
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Plant Flavoprotein Photoreceptors
Christie, J. M., Blackwood, L., Petersen, J., & Sullivan, S. (2015) · Plant and Cell Physiology · doi:10.1093/pcp/pcu196 · Cited by 294 · see citing works
Roberts, D., Pedmale, U. V., Morrow, J., Sachdev, S., Lechner, E., Tang, X., Zheng, N., Hannink, M., Genschik, P., & Liscum, E. (2011) · The Plant Cell · doi:10.1105/tpc.111.087999 · Cited by 150 · see citing works
Chloroplast Accumulation Response Enhances Leaf Photosynthesis and Plant Biomass Production
Gotoh, E., Suetsugu, N., Yamori, W., Ishishita, K., Kiyabu, R., Fukuda, M., Higa, T., Shirouchi, B., & Wada, M. (2018) · Plant Physiology · doi:10.1104/pp.18.00484 · Cited by 128 · see citing works
In Vivo Phosphorylation Site Mapping and Functional Characterization of Arabidopsis Phototropin 1
Sullivan, S., Thomson, C. E., Lamont, D. J., Jones, M. A., & Christie, J. M. (2008) · Molecular Plant · doi:10.1093/mp/ssm017 · Cited by 107 · see citing works
Phototropism: at the crossroads of light-signaling pathways
Goyal, A., Szarzynska, B., & Fankhauser, C. (2013) · Trends in Plant Science · doi:10.1016/j.tplants.2013.03.002 · Cited by 109 · see citing works
Wan, Y.-L., Eisinger, W., Ehrhardt, D., Kubitscheck, U., Baluska, F., & Briggs, W. (2008) · Molecular Plant · doi:10.1093/mp/ssm011 · Cited by 105 · see citing works
Phytochrome Kinase Substrate 4 is phosphorylated by the phototropin 1 photoreceptor
Demarsy, E., Schepens, I., Okajima, K., Hersch, M., Bergmann, S., Christie, J., Shimazaki, K., Tokutomi, S., & Fankhauser, C. (2012) · The EMBO Journal · doi:10.1038/emboj.2012.186 · Cited by 94 · see citing works
Self-citationShining Light on the Function of NPH3/RPT2-Like Proteins in Phototropin Signaling
Christie, J. M., Suetsugu, N., Sullivan, S., & Wada, M. (2018) · Plant Physiology · doi:10.1104/pp.17.00835 · Cited by 81 · see citing works
Haga, K., Tsuchida-Mayama, T., Yamada, M., & Sakai, T. (2015) · The Plant Cell · doi:10.1105/tpc.15.00178 · Cited by 66 · see citing works
Molecular basis of chloroplast photorelocation movement
Kong, S.-G., & Wada, M. (2016) · Journal of Plant Research · doi:10.1007/s10265-016-0788-1 · Cited by 50 · see citing works
Deetiolation Enhances Phototropism by Modulating NON-PHOTOTROPIC HYPOCOTYL3 Phosphorylation Status
Sullivan, S., Kharshiing, E., Laird, J., Sakai, T., & Christie, J. M. (2019) · Plant Physiology · doi:10.1104/pp.19.00206 · Cited by 33 · see citing works
Zhao, X., Zhao, Q., Xu, C., Wang, J., Zhu, J., Shang, B., & Zhang, X. (2018) · Journal of Integrative Plant Biology · doi:10.1111/jipb.12639 · Cited by 32 · see citing works
The action of enhancing weak light capture via phototropic growth and chloroplast movement in plants
Xin, G.-Y., Li, L.-P., Wang, P.-T., Li, X.-Y., Han, Y.-J., & Zhao, X. (2022) · Stress Biology · doi:10.1007/s44154-022-00066-x · Cited by 17 · see citing works
Wang, J., Liang, Y.-P., Zhu, J.-D., Wang, Y.-X., Yang, M.-Y., Yan, H.-R., Lv, Q.-Y., Cheng, K., Zhao, X., & Zhang, X. (2021) · Frontiers in Plant Science · doi:10.3389/fpls.2021.704618 · Cited by 15 · see citing works
Suetsugu, N., & Wada, M. (2017) · International Journal of Molecular Sciences · doi:10.3390/ijms18071469 · Cited by 7 · see citing works
- Authors
- Inoue, S.-I., Kinoshita, T., Takemiya, A., Doi, M., & Shimazaki, K.-I.
- Affiliations
- Kyushu University (JP)
- Venue
- Molecular Plant
- Publisher
- Elsevier BV
- Published
- 2008-01
- Volume
- 1
- Issue
- 1
- Pages
- 15-26
- ISSN
- 1674-2052 (ISSN-L)
- OpenAlex
- W2082598282
- Cited by
- 156
- References
- 6
- Citations by year
- 2026: 6 · 2025: 4 · 2024: 9 · 2023: 5 · 2022: 4 · 2021: 11 · 2020: 12 · 2019: 6 · 2018: 10 · 2017: 5 · 2016: 10 · 2015: 7 · 2014: 14 · 2013: 7 · 2012: 15
- FWCI
- 10.53
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Molecular Plant (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Ministry of Education, Culture, Sports, Science and Technology (14704003, 16207003, 17084005)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Light effects on plants
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
- Keywords
- blue light, phototropism, NPH3, petiole orientation, red light, plasma membrane localization, coiled-coil domain, light perception, Arabidopsis thaliana
- MeSH terms
- Gravitation, Light, Morphogenesis, Photosynthesis, Protoplasts, Recombinant Fusion Proteins, Ultraviolet Rays, Arabidopsis, Genes, Reporter, Plant Leaves, Arabidopsis Proteins, Phototropins
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