The Subcellular Localization and Blue-Light-Induced Movement of Phototropin 1-GFP in Etiolated Seedlings of Arabidopsis thalianaw
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Phototropin 1 (phot1) is a photoreceptor for phototropism, chloroplast movement, stomatal opening, leaf expansion, and solar tracking in response to blue light. Following earlier work with PHOT1::GFP (Sakamoto and Briggs, 2002), we investigated the pattern of cellular and subcellular localization of phot1 in 3- 4-d-old etiolated seedlings of Arabidopsis thalinana. As expressed from native upstream sequences, the PHOT1::GFP fusion protein is expressed strongly in the abaxial tissues of the cotyledons and in the elongating regions of the hypocotyl. It is moderately expressed in the shoot/root transition zone and in cells near the root apex. A fluorescence signal is undetectable in the root epidermis, root cap, and root apical meristem itself. The plasma membranes of mesophyll cells near the cotyledon margin appear labeled uniformly but cross-walls created by recent cell divisions are more strongly labeled. The pattern of labeling of individual cell types varies with cell type and developmental stage. Blue-light treatment causes PHOT1::GFP, initially relatively evenly distributed at the plasma membrane, to become reorganized into a distinct mosaic with strongly labeled punctate areas and other areas completely devoid of fluorescence--a phenomenon best observed in cortical cells in the hypocotyl elongation region. Concomitant with or following this reorganization, PHOT1::GFP moves into the cytoplasm in all cell types investigated except for guard cells. It disappears from the cytoplasm by an unidentified mechanism after several hours in darkness. Neither its appearance in the cytoplasm nor its eventual disappearance in darkness is prevented by the translation inhibitor cycloheximide, although the latter process is retarded. We hypothesize that blue-light-induced phot1 re-localization modulates blue-light-activated signal transduction.
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Under blue light irradiation, part of PHOT1 detaches from the cell membrane and disperses in the cytoplasm, whereas some PHOT2 proteins can be coupled to the Golgi apparatus.
“Under blue light irradiation, part of the PHOT1 protein detaches from the cell membrane and disperses in the cytoplasm, in contrast, some PHOT2 proteins can be coupled to the Golgi apparatus (Sakamoto and Briggs, 2002; Kong et al., 2006; Wan et al., 2008).”
From The action of enhancing weak light capture via phototropic growth and chloroplast movement in plants (2022) · cites this paper as Wan et al., 2008
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The action of enhancing weak light capture via phototropic growth and chloroplast movement in plants
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- Authors
- Wan, Y.-L., Eisinger, W., Ehrhardt, D., Kubitscheck, U., Baluska, F., & Briggs, W.
- Affiliations
- Santa Clara University (US)
- Carnegie Institution for Science (US)
- University of Bonn (DE)
- Corresponding author
- Briggs, Winslow
- Venue
- Molecular Plant
- Publisher
- Elsevier BV
- Published
- 2008-01
- Volume
- 1
- Issue
- 1
- Pages
- 103-117
- ISSN
- 1674-2052 (ISSN-L)
- OpenAlex
- W2157978223
- Cited by
- 105
- References
- 44
- Citations by year
- 2026: 2 · 2025: 5 · 2024: 1 · 2023: 3 · 2022: 5 · 2021: 3 · 2020: 3 · 2019: 5 · 2018: 7 · 2017: 1 · 2016: 3 · 2015: 5 · 2014: 11 · 2013: 7 · 2012: 14
- FWCI
- 9.71
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Molecular Plant (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Science Foundation
- National Institutes of Health
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Light effects on plants
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
- Keywords
- phototropin 1, subcellular localization, blue light, phototropism, etiolated seedlings, Arabidopsis thaliana, GFP fusion protein, plasma membrane, hypocotyl elongation, cotyledons, guard cells, cycloheximide, signal transduction, photoreceptors
- MeSH terms
- Image Processing, Computer-Assisted, Light, Recombinant Fusion Proteins, Subcellular Fractions, Arabidopsis, Genes, Reporter, Plant Roots, Plant Shoots, Hypocotyl, Cotyledon, Seedlings, Green Fluorescent Proteins, Phototropins
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