Phot2‐regulated relocation of NPH3 mediates phototropic response to high‐intensity blue light in Arabidopsis thaliana
Open accessAbstract
Two redundant blue‐light receptors, known as phototropins (phot1 and phot2), influence a variety of physiological responses, including phototropism, chloroplast positioning, and stomatal opening in Arabidopsis thaliana. Whereas phot1 functions in both low‐ and high‐intensity blue light (HBL), phot2 functions primarily in HBL. Here, we aimed to elucidate phot2‐specific functions by screening for HBL‐insensitive mutants among mutagenized Arabidopsis phot1 mutants. One of the resulting phot2 signaling associated (p2sa) double mutants, phot1 p2sa2, exhibited phototropic defects that could be restored by constitutively expressing NON‐PHOTOTROPIC HYPOCOTYL 3 (NPH3), indicating that P2SA2 was allelic to NPH3. It was observed that NPH3‐GFP signal mainly localized to and clustered on the plasma membrane in darkness. This NPH3 clustering on the plasma membrane was not affected by mutations in genes encoding proteins that interact with NPH3, including PHOT1, PHOT2 and ROOT PHOTOTROPISM 2 (RPT2). However, the HBL irradiation‐mediated release of NPH3 proteins into the cytoplasm was inhibited in phot1 mutants and enhanced in phot2 and rpt2‐2 mutants. Furthermore, HBL‐induced hypocotyl phototropism was enhanced in phot1 mutants and inhibited in the phot2 and rpt2‐2 mutants. Our findings indicate that phot1 regulates the dissociation of NPH3 from the plasma membrane, whereas phot2 mediates the stabilization and relocation of NPH3 to the plasma membrane to acclimate to HBL.
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The process of NPH3 leaving the plasma membrane is regulated by PHOT1, whereas PHOT2 regulates the stability and repositioning of NPH3 to the plasma membrane to adapt to high-fluence blue light.
“The process of NPH3 leaving the plasma membrane is regulated by PHOT1, whereas PHOT2 regulates the stability and repositioning of NPH3 to the plasma membrane to adapt to high-fluence blue light (Zhao et al., 2018).”
From The action of enhancing weak light capture via phototropic growth and chloroplast movement in plants (2022) · cites this paper as Zhao et al., 2018
The Discussion (page unavailable) says phot1 regulates NPH3’s dissociation from the plasma membrane and phot2 regulates its return during continuous high-intensity blue light. But it identifies phot1—not NPH3—as the protein whose stability phot2 may regulate. (LLM, conf=0.90)
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Zhao, Q., Zhu, J., Li, N., Wang, X., Zhao, X., & Zhang, X. (2020) · Journal of Integrative Plant Biology · doi:10.1111/jipb.12813 · Cited by 28 · see citing works
Self-citationThe action of enhancing weak light capture via phototropic growth and chloroplast movement in plants
Xin, G.-Y., Li, L.-P., Wang, P.-T., Li, X.-Y., Han, Y.-J., & Zhao, X. (2022) · Stress Biology · doi:10.1007/s44154-022-00066-x · Cited by 17 · see citing works
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- Authors
- Zhao, X., Zhao, Q., Xu, C., Wang, J., Zhu, J., Shang, B., & Zhang, X.
- Affiliations
- Henan University (CN)
- Institute of Plant Stress Biology State Key Laboratory of Cotton Biology School of Life Sciences Henan University Kaifeng 475004 China
- Corresponding author
- Zhang, Xiao
- Venue
- Journal of Integrative Plant Biology
- Publisher
- Wiley
- Published
- 2018-04-30
- Volume
- 60
- Issue
- 7
- Pages
- 562-577
- ISSN
- 0577-7496 (ISSN-L)
- 1672-9072
- 1744-7909
- OpenAlex
- W2794340967
- Cited by
- 32
- References
- 38
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 6 · 2023: 2 · 2022: 3 · 2021: 6 · 2020: 6 · 2019: 4 · 2018: 2
- FWCI
- 3.98
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Integrative Plant Biology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (31670289, 31570294)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Light effects on plants
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
- Keywords
- phot2, phot1, NPH3, phototropism, blue light receptor, plasma membrane localization, Rpt2, Arabidopsis thaliana
- MeSH terms
- Cell Membrane, Light, Mutation, Recombinant Fusion Proteins, Subcellular Fractions, Signal Transduction, Arabidopsis, Gene Expression Regulation, Plant, Phototropism, Hypocotyl, Protein Transport, Arabidopsis Proteins, Plants, Genetically Modified, Seedlings, Green Fluorescent Proteins, Phototropins, Etiolation
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