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Cited by 448

The DOI matches its Crossref record; 42 of the 42 references it cites were found in a registry or an index.

Molecular cloning and characterization of human estrogen receptor betacx: a potential inhibitor ofestrogen action in human

Open access
Ogawa, S·1998·Nucleic Acids Research
DOI 10.1093/nar/26.15.3505
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Abstract

We have identified and characterized a novel human estrogen receptor (ER) beta isoform, ERbetacx, which is truncated at the C-terminal region but has an extra 26 amino acids due to alternative splicing. The ERbetacx transcript is expressed in testis, ovary, thymus and prostate as well as in human cultured cell lines such as HEC-1, HOS-TE85 and Saos-2 cells. ERbetacx protein is also immunodetectable in these human cells. Biochemical analysis reveals that the average dissociation constants ( K d) of ERalpha and ERbeta for 17beta-estradiol (E2) are 0.2 and 0.6 nM respectively, but ERbetacx has no ligand binding ability. ERalpha and ERbeta proteins bind to the estrogen response element, whereas ERbetacx does not form any shifted complex in gel shift assays. In a transient expression assay, ERbetacx shows no ligand-dependent transactivation ability of a basal promoter and also cannot interact with a cofactor, TIF1alpha, in the presence or absence of E2. ERbetacx preferentially forms a heterodimer with ERalpha rather than that with ERbeta, inhibiting DNA binding by ERalpha. Interestingly, however, it shows a significant dominant negative activity only against ERalpha transactivation. Thus, this study indicates that ERbetacx potentially inhibits ERalpha-mediated estrogen action and that alternative splicing of the C-terminal region and its inhibitory properties are characteristic of several members of nuclear receptor isoforms.

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Metadata

Authors
Ogawa, S.
Venue
Nucleic Acids Research
Publisher
Oxford University Press (OUP)
Published
1998-08-01
Volume
26
Issue
15
Pages
3505-3512
ISSN
  • 0305-1048 (ISSN-L)
  • 1362-4962
DOI
10.1093/nar/26.15.3505
OpenAlex
W2116325136
Cited by
448
References
42
FWCI
11.00
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: Molecular cloning and characterization of human estrogen receptor betacx: a potential inhibitor ofestrogen action in human. https://app.cochat.ai/papers/10.1093/nar/26.15.3505
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Ogawa, S. (1998). Molecular cloning and characterization of human estrogen receptor betacx: a potential inhibitor ofestrogen action in human. Nucleic Acids Research. https://doi.org/10.1093/nar/26.15.3505

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

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