Cited by 2,829Genomics and Phylogenetic StudiesRNA Research and SplicingGenomics and Chromatin Dynamics

AUGUSTUS: ab initio prediction of alternative transcripts

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Stanke, M., Keller, O., Gunduz, I., Hayes, A., Waack, S., Morgenstern, B.·2006·Nucleic Acids Research

Abstract

AUGUSTUS is a software tool for gene prediction in eukaryotes based on a Generalized Hidden Markov Model, a probabilistic model of a sequence and its gene structure. Like most existing gene finders, the first version of AUGUSTUS returned one transcript per predicted gene and ignored the phenomenon of alternative splicing. Herein, we present a WWW server for an extended version of AUGUSTUS that is able to predict multiple splice variants. To our knowledge, this is the first ab initio gene finder that can predict multiple transcripts. In addition, we offer a motif searching facility, where user-defined regular expressions can be searched against putative proteins encoded by the predicted genes. The AUGUSTUS web interface and the downloadable open-source stand-alone program are freely available from http://augustus.gobics.de.

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  • Gene-finding tools such as Augustus can incorporate RNA-seq data to better annotate protein-coding transcripts, but perform worse on non-coding transcripts.

    “Gene-finding tools such as Augustus [28] can incorporate RNA-seq data to better annotate protein-coding transcripts, but perform worse on non-coding transcripts [29].”

    From A survey of best practices for RNA-seq data analysis (2016) · cites this paper as [28]

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  1. Gadani,F., Hayes,A., Opperman,C.H., Lommel,S.A., Sosinski,B.R., Burke,M., Hi,L., Brierly,R., Salstead,A. and Heer,J. (2003) Large scale genome sequencing and analysis of Nicotiana tabacum: the tobacco genome initiative. 5e`mes Journe´es Scientifiques du Tabac de Bergerac, pp. 117-130.

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Authors
Stanke, M., Keller, O., Gunduz, I., Hayes, A., Waack, S., & Morgenstern, B.
Affiliations
  • University of Göttingen (DE)
  • Altria (United States) (US)
Corresponding author
Stanke, M.
Venue
Nucleic Acids Research
Published
2006-07-01
Volume
34
Issue
Web Server
Pages
W435-W439
ISSN
  • 0305-1048 (ISSN-L)
  • 1362-4962
OpenAlex
W1996700726
Cited by
2,829
References
24
Citations by year
2026: 305 · 2025: 464 · 2024: 412 · 2023: 325 · 2022: 287 · 2021: 307 · 2020: 178 · 2019: 160 · 2018: 83 · 2017: 77 · 2016: 59 · 2015: 39 · 2014: 43 · 2013: 24 · 2012: 15
FWCI
2.99
Type
journal article
Language
en
Open access
Licence
cc-by-nc
Locations
  • Nucleic Acids Research (publishedVersion, cc-by-nc)
  • PubMed (publishedVersion)
  • GoeScholar The Publication Server of the Georg-August-Universität Göttingen (Georg-August-Universität Göttingen) (submittedVersion, cc-by)
Funders
  • Deutscher Akademischer Austauschdienst
  • Bundesministerium für Bildung und Forschung (01AK803G)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Phylogenetic Studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
Keywords
Augustus, gene prediction, alternative splicing, splice variants, generalized hidden Markov models, eukaryotic gene structure, web server, protein-coding genes
MeSH terms
Animals, Exons, Genes, Humans, Introns, Markov Chains, Proteins, Software, User-Computer Interface, Gene Expression, Alternative Splicing, Internet

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