AUGUSTUS: ab initio prediction of alternative transcripts
Open accessAbstract
AUGUSTUS is a software tool for gene prediction in eukaryotes based on a Generalized Hidden Markov Model, a probabilistic model of a sequence and its gene structure. Like most existing gene finders, the first version of AUGUSTUS returned one transcript per predicted gene and ignored the phenomenon of alternative splicing. Herein, we present a WWW server for an extended version of AUGUSTUS that is able to predict multiple splice variants. To our knowledge, this is the first ab initio gene finder that can predict multiple transcripts. In addition, we offer a motif searching facility, where user-defined regular expressions can be searched against putative proteins encoded by the predicted genes. The AUGUSTUS web interface and the downloadable open-source stand-alone program are freely available from http://augustus.gobics.de.
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Gene-finding tools such as Augustus can incorporate RNA-seq data to better annotate protein-coding transcripts, but perform worse on non-coding transcripts.
“Gene-finding tools such as Augustus [28] can incorporate RNA-seq data to better annotate protein-coding transcripts, but perform worse on non-coding transcripts [29].”
From A survey of best practices for RNA-seq data analysis (2016) · cites this paper as [28]
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- Authors
- Stanke, M., Keller, O., Gunduz, I., Hayes, A., Waack, S., & Morgenstern, B.
- Affiliations
- University of Göttingen (DE)
- Altria (United States) (US)
- Corresponding author
- Stanke, M.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2006-07-01
- Volume
- 34
- Issue
- Web Server
- Pages
- W435-W439
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W1996700726
- Cited by
- 2,829
- References
- 24
- Citations by year
- 2026: 305 · 2025: 464 · 2024: 412 · 2023: 325 · 2022: 287 · 2021: 307 · 2020: 178 · 2019: 160 · 2018: 83 · 2017: 77 · 2016: 59 · 2015: 39 · 2014: 43 · 2013: 24 · 2012: 15
- FWCI
- 2.99
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by-nc
- Locations
- Nucleic Acids Research (publishedVersion, cc-by-nc)
- PubMed (publishedVersion)
- GoeScholar The Publication Server of the Georg-August-Universität Göttingen (Georg-August-Universität Göttingen) (submittedVersion, cc-by)
- Funders
- Deutscher Akademischer Austauschdienst
- Bundesministerium für Bildung und Forschung (01AK803G)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Phylogenetic Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Keywords
- Augustus, gene prediction, alternative splicing, splice variants, generalized hidden Markov models, eukaryotic gene structure, web server, protein-coding genes
- MeSH terms
- Animals, Exons, Genes, Humans, Introns, Markov Chains, Proteins, Software, User-Computer Interface, Gene Expression, Alternative Splicing, Internet
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