The DOI matches its Crossref record; 45 of the 45 references it cites were found in a registry or an index.
Advances in CRISPR-Cas9 genome engineering: lessons learned from RNA interference
Open accessAbstract
The discovery that the machinery of the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-Cas9 bacterial immune system can be re-purposed to easily create deletions, insertions and replacements in the mammalian genome has revolutionized the field of genome engineering and re-invigorated the field of gene therapy. Many parallels have been drawn between the newly discovered CRISPR-Cas9 system and the RNA interference (RNAi) pathway in terms of their utility for understanding and interrogating gene function in mammalian cells. Given this similarity, the CRISPR-Cas9 field stands to benefit immensely from lessons learned during the development of RNAi technology. We examine how the history of RNAi can inform today's challenges in CRISPR-Cas9 genome engineering such as efficiency, specificity, high-throughput screening and delivery for in vivo and therapeutic applications.
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Citations
Cites
RNA-Guided Human Genome Engineering via Cas9
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Circular RNAs are a large class of animal RNAs with regulatory potency
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Genome-scale CRISPR-Cas9 knockout screening in human cells
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“Targeting of promoters can also be achieved using a catalytically inactive Cas9 in combination with sgRNA (CRISPRi) to precisely interfere with the transcriptional machinery (124).”
BackgroundDNA targeting specificity of RNA-guided Cas9 nucleases
Hsu, P. D., Scott, D. A., Weinstein, J. A., Ran, F. A., Konermann, S., Agarwala, V., Li, Y., Fine, E. J., Wu, X., Shalem, O., et al. (2013) · Nature Biotechnology · doi:10.1038/nbt.2647 · Cited by 4,741
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High-Frequency off-Target Mutagenesis Induced by CRISPR-Cas Nucleases in Human Cells
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“This proves a difficulty for both technologies, as efficiency of shRNA-mediated knockdown varies for different platforms but never reaches complete knockdown for all vectors targeting a specific gene (91,92) and the observed frequency of CRISPR-Cas9-based gene inactivation using single-copy sgRNAs shows great variabil…”
Background
Cited by
Applications of CRISPR technologies in research and beyond
Barrangou, R., & Doudna, J. A. (2016) · Nature Biotechnology · doi:10.1038/nbt.3659 · Cited by 1,005
Self-citationSystematic comparison of CRISPR/Cas9 and RNAi screens for essential genes
Morgens, D. W., Deans, R. M., Li, A., & Bassik, M. C. (2016) · Nature Biotechnology · doi:10.1038/nbt.3567 · Cited by 401
A decade of discovery: CRISPR functions and applications
Barrangou, R., & Horvath, P. (2017) · Nature Microbiology · doi:10.1038/nmicrobiol.2017.92 · Cited by 319
Self-citationPlasticity in designing PROTACs for selective and potent degradation of HDAC6
Yang, H., Lv, W., He, M., Deng, H., Li, H., Wu, W., & Rao, Y. (2019) · Chemical Communications · doi:10.1039/c9cc08509b · Cited by 108
Accelerating the search for the missing proteins in the human proteome
Baker, M. S., Ahn, S. B., Mohamedali, A., Islam, M. T., Cantor, D., Verhaert, P. D., Fanayan, S., Sharma, S., Nice, E. C., Connor, M., et al. (2017) · Nature Communications · doi:10.1038/ncomms14271 · Cited by 100
Metadata
- Authors
- Barrangou, R., Birmingham, A., Wiemann, S., Beijersbergen, R. L., Hornung, V., & Smith, A. V. B.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2015-03-23
- Volume
- 43
- Issue
- 7
- Pages
- 3407-3419
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W2093714761
- Cited by
- 165
- References
- 45
- FWCI
- 9.52
- Type
- journal article
- Language
- en
- Open access
- gold
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- Barrangou, R., Birmingham, A., Wiemann, S., Beijersbergen, R. L., Hornung, V., & Smith, A. V. B. (2015). Advances in CRISPR-Cas9 genome engineering: lessons learned from RNA interference. Nucleic Acids Research. https://doi.org/10.1093/nar/gkv226
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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