Visualization of self-delivering hydrophobically modified siRNA cellular internalization
Open accessAbstract
siRNAs are a new class of therapeutic modalities with promising clinical efficacy that requires modification or formulation for delivery to the tissue and cell of interest. Conjugation of siRNAs to lipophilic groups supports efficient cellular uptake by a mechanism that is not well characterized. Here we study the mechanism of internalization of asymmetric, chemically stabilized, cholesterol-modified siRNAs (sd-rxRNAs®) that efficiently enter cells and tissues without the need for formulation. We demonstrate that uptake is rapid with significant membrane association within minutes of exposure followed by the formation of vesicular structures and internalization. Furthermore, sd-rxRNAs are internalized by a specific class of early endosomes and show preferential association with epidermal growth factor (EGF) but not transferrin (Tf) trafficking pathways as shown by live cell TIRF and structured illumination microscopy (SIM). In fixed cells, we observe ∼25% of sd-rxRNA co-localizing with EGF and <5% with Tf, which is indicative of selective endosomal sorting. Likewise, preferential sd-rxRNA co-localization was demonstrated with EEA1 but not RBSN-containing endosomes, consistent with preferential EGF-like trafficking through EEA1-containing endosomes. sd-rxRNA cellular uptake is a two-step process, with rapid membrane association followed by internalization through a selective, saturable subset of the endocytic process. However, the mechanistic role of EEA1 is not yet known. This method of visualization can be used to better understand the kinetics and mechanisms of hydrophobic siRNA cellular uptake and will assist in further optimization of these types of compounds for therapeutic intervention.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Intracellular distribution followed a perinuclear localization pattern characteristic of oligonucleotides.
“Intracellular distribution followed a perinuclear localization pattern that is characteristic of oligonucleotides11.”
From A divalent siRNA chemical scaffold for potent and sustained modulation of gene expression throughout the central nervous system (2019) · cites this paper as [11]
The Results section ('sd-rxRNA quickly internalizes through endocytic pathways following membrane association'; page unavailable) reports vesicles carrying the studied oligonucleotide in the perinuclear space. It does not establish that this localization pattern is characteristic of oligonucleotides generally. (LLM, conf=0.85)
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Huang, D. W., Sherman, B. T., & Lempicki, R. A. (2009) · Nucleic Acids Research · doi:10.1093/nar/gkn923 · Cited by 14,108 · see citing works
Knocking down barriers: advances in siRNA delivery
Whitehead, K. A., Langer, R., & Anderson, D. G. (2009) · Nature Reviews Drug Discovery · doi:10.1038/nrd2742 · Cited by 3,115 · see citing works
Therapeutic silencing of an endogenous gene by systemic administration of modified siRNAs
Soutschek, J., Akinc, A., Bramlage, B., Charissé, K., Constien, R., Donoghue, M., Elbashir, S. M., Geick, A., Hadwiger, P., Harborth, J., et al. (2004) · Nature · doi:10.1038/nature03121 · Cited by 2,189 · see citing works
Rational design of cationic lipids for siRNA delivery
Semple, S. C., Akinc, A., Chen, J., Sandhu, A. P., Mui, B. L., Cho, C. K., Sah, D. W. Y., Stebbing, D., Crosley, E. J., Yaworski, E., et al. (2010) · Nature Biotechnology · doi:10.1038/nbt.1602 · Cited by 1,822 · cited 1× · see citing works
“Despite years of exploration, only a limited number of formulations and conjugates have progressed to the clinic: lipid nanoparticles (LNPs) (16,17), dynamic polyconjugates (18), dextrins (19), N-acetylgalactosamine (GalNAc) (8) and cholesterol (9).”
BackgroundDelivery materials for siRNA therapeutics
Kanasty, R., Dorkin, J. R., Vegas, A., & Anderson, D. (2013) · Nature Materials · doi:10.1038/nmat3765 · Cited by 1,800 · see citing works
Gilleron, J., Querbes, W., Zeigerer, A., Borodovsky, A., Marsico, G., Schubert, U., Manygoats, K., Seifert, S., Andree, C., Stöter, M., et al. (2013) · Nature Biotechnology · doi:10.1038/nbt.2612 · Cited by 1,681 · see citing works
Endosomal escape pathways for delivery of biologicals
Varkouhi, A. K., Scholte, M., Storm, G., & Haisma, H. J. (2011) · Journal of Controlled Release · doi:10.1016/j.jconrel.2010.11.004 · Cited by 1,561 · see citing works
A new vital stain for visualizing vacuolar membrane dynamics and endocytosis in yeast.
Vida, T. A., & Emr, S. D. (1995) · The Journal of Cell Biology · doi:10.1083/jcb.128.5.779 · Cited by 1,364 · see citing works
Nair, J. K., Willoughby, J. L. S., Chan, A., Charisse, K., Alam, M. R., Wang, Q., Hoekstra, M., Kandasamy, P., Kel’in, A. V., Milstein, S., et al. (2014) · Journal of the American Chemical Society · doi:10.1021/ja505986a · Cited by 1,141 · see citing works
Sönnichsen, B., Renzis, S. D., Nielsen, E., Rietdorf, J., & Zerial, M. (2000) · The Journal of Cell Biology · doi:10.1083/jcb.149.4.901 · Cited by 1,013 · see citing works
Gibbings, D., Ciaudo, C., Erhardt, M., & Voinnet, O. (2009) · Nature Cell Biology · doi:10.1038/ncb1929 · Cited by 990 · see citing works
Mechanisms and optimization of in vivo delivery of lipophilic siRNAs
Wolfrum, C., Shi, S., Jayaprakash, K. N., Jayaraman, M., Wang, G., Pandey, R. K., Rajeev, K. G., Nakayama, T., Charrise, K., Ndungo, E. M., et al. (2007) · Nature Biotechnology · doi:10.1038/nbt1339 · Cited by 974 · see citing works
Lipid-like materials for low-dose, in vivo gene silencing
Love, K. T., Mahon, K. P., Levins, C. G., Whitehead, K. A., Querbes, W., Dorkin, J. R., Qin, J., Cantley, W., Qin, L. L., Racie, T., et al. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0910603106 · Cited by 969 · see citing works
Efficiency of siRNA delivery by lipid nanoparticles is limited by endocytic recycling
Sahay, G., Querbes, W., Alabi, C., Eltoukhy, A., Sarkar, S., Zurenko, C., Karagiannis, E., Love, K., Chen, D., Zoncu, R., et al. (2013) · Nature Biotechnology · doi:10.1038/nbt.2614 · Cited by 948 · see citing works
Pharmacokinetics, biodistribution and cell uptake of antisense oligonucleotides
Geary, R. S., Norris, D. A., Yu, R. Z., & Bennett, C. F. (2015) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2015.01.008 · Cited by 846 · see citing works
Knocking down disease: a progress report on siRNA therapeutics
Wittrup, A., & Lieberman, J. (2015) · Nature Reviews Genetics · doi:10.1038/nrg3978 · Cited by 810 · see citing works
Endocytosis and intracellular trafficking of ErbBs
Sorkin, A. D., & Goh, L. K. (2008) · Experimental Cell Research · doi:10.1016/j.yexcr.2008.07.029 · Cited by 756 · see citing works
Visualizing lipid-formulated siRNA release from endosomes and target gene knockdown
Wittrup, A., Ai, A., Liu, X., Hamar, P., Trifonova, R., Charisse, K., Manoharan, M., Kirchhausen, T., & Lieberman, J. (2015) · Nature Biotechnology · doi:10.1038/nbt.3298 · Cited by 693 · see citing works
Dynamic PolyConjugates for targeted in vivo delivery of siRNA to hepatocytes
Rozema, D. B., Lewis, D. L., Wakefield, D. H., Wong, S. C., Klein, J. J., Roesch, P. L., Bertin, S. L., Reppen, T. W., Chu, Q., Blokhin, A. V., et al. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0703778104 · Cited by 658 · see citing works
Lakadamyali, M., Rust, M., & Zhuang, X. (2006) · Cell · doi:10.1016/j.cell.2005.12.038 · Cited by 589 · see citing works
The endocytic pathway mediates cell entry of dsRNA to induce RNAi silencing
Saleh, M., van Rij, R. P., Hekele, A., Gillis, A. N., Foley, E., O’Farrell, P. H., & Andino, R. (2006) · Nature Cell Biology · doi:10.1038/ncb1439 · Cited by 561 · see citing works
Spatial control of EGF receptor activation by reversible dimerization on living cells
Chung, I., Akita, R. W., VANDLEN, R. L., Toomre, D. K., Schlessinger, J., & Mellman, I. (2010) · Nature · doi:10.1038/nature08827 · Cited by 538 · see citing works
Hrs regulates multivesicular body formation via ESCRT recruitment to endosomes
Bache, K. G., Brech, A., Mehlum, A., & Stenmark, H. (2003) · The Journal of Cell Biology · doi:10.1083/jcb.200302131 · Cited by 465 · see citing works
Laukaitis, C. M., Webb, D. J., Donais, K., & Horwitz, A. F. (2001) · The Journal of Cell Biology · doi:10.1083/jcb.153.7.1427 · Cited by 455 · see citing works
Systems survey of endocytosis by multiparametric image analysis
Collinet, C., Stöter, M., Bradshaw, C. R., Samusik, N., Rink, J. C., Kenski, D. M., Habermann, B. H., Buchholz, F., Henschel, R., Mueller, M., et al. (2010) · Nature · doi:10.1038/nature08779 · Cited by 446 · see citing works
Chemically modified siRNA: tools and applications
Watts, J. K., Deleavey, G. F., & Damha, M. J. (2008) · Drug Discovery Today · doi:10.1016/j.drudis.2008.05.007 · Cited by 431 · see citing works
DiFiglia, M., Sena‐Esteves, M., Chase, K. O., Sapp, E., Pfister, E., Sass, M., Yoder, J., Reeves, P., Pandey, R. K., Rajeev, K. G., et al. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0708285104 · Cited by 417 · see citing works
Hrs recruits clathrin to early endosomes
Raiborg, C., Bache, K. G., Mehlum, A., Stang, E., & Stenmark, H. (2001) · The EMBO Journal · doi:10.1093/emboj/20.17.5008 · Cited by 389 · see citing works
Nielsen, E., Christoforidis, S., Uttenweiler‐Joseph, S., Miączyńska, M., Dewitte, F., Wilm, M. S., Hoflack, B., & Zerial, M. (2000) · The Journal of Cell Biology · doi:10.1083/jcb.151.3.601 · Cited by 383 · see citing works
Patel, P. C., Giljohann, D. A., Daniel, W. L., Zheng, D., Prigodich, A. E., & Mirkin, C. A. (2010) · Bioconjugate Chemistry · doi:10.1021/bc1002423 · Cited by 366 · see citing works
Daro, E., van der Sluijs, P., Galli, T., & Mellman, I. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.18.9559 · Cited by 340 · see citing works
Silencing by small RNAs is linked to endosomal trafficking
Lee, Y. S., Pressman, S., Andress, A. P., Kim, K., White, J. L., Cassidy, J. J., Li, X., Lubell, K., Lim, D., Cho, I. S., et al. (2009) · Nature Cell Biology · doi:10.1038/ncb1930 · Cited by 338 · see citing works
A Phosphoinositide Switch Controls the Maturation and Signaling Properties of APPL Endosomes
Zoncu, R., Perera, R. M., Balkin, D. M., Pirruccello, M., Toomre, D. K., & Camilli, P. V. D. (2009) · Cell · doi:10.1016/j.cell.2009.01.032 · Cited by 336 · see citing works
Sehgal, A., Barros, S. A., Ivanciu, L., Cooley, B. C., Qin, J., Racie, T., Hettinger, J. L., Carioto, M., Jiang, Y., Brodsky, J., et al. (2015) · Nature Medicine · doi:10.1038/nm.3847 · Cited by 307 · see citing works
A status report on RNAi therapeutics
Vaishnaw, A. K., Gollob, J., Gamba‐Vitalo, C., Hutabarat, R. M., Sah, D. W. Y., Meyers, R. E., de Fougerolles, T., & Maraganore, J. M. (2010) · Silence · doi:10.1186/1758-907x-1-14 · Cited by 297 · see citing works
Koller, E., Vincent, T. M., Chappell, A. E., De, S., Manoharan, M., & Bennett, C. F. (2011) · Nucleic Acids Research · doi:10.1093/nar/gkr089 · Cited by 284 · see citing works
A distinct class of endosome mediates clathrin-independent endocytosis to the Golgi complex
Nichols, B. J. (2002) · Nature Cell Biology · doi:10.1038/ncb787 · Cited by 258 · see citing works
Distinct Roles for Tsg101 and Hrs in Multivesicular Body Formation and Inward Vesiculation
Razi, M., & Futter, C. E. (2006) · Molecular Biology of the Cell · doi:10.1091/mbc.e05-11-1054 · Cited by 255 · see citing works
Epidermal Growth Factor and Membrane Trafficking
Barbieri, M. A., Roberts, R. L., Gumusboga, A., Highfield, H. A., Alvarez-Domı́nguez, C., Wells, A. H., & Stahl, P. D. (2000) · The Journal of Cell Biology · doi:10.1083/jcb.151.3.539 · Cited by 240 · see citing works
The rough endoplasmatic reticulum is a central nucleation site of siRNA‐mediated RNA silencing
Stalder, L., Heusermann, W., Sokol, L., Trojer, D., Wirz, J., Hean, J., Fritzsche, A., Aeschimann, F., Pfanzagl, V., Basselet, P., et al. (2013) · The EMBO Journal · doi:10.1038/emboj.2013.52 · Cited by 229 · see citing works
Pharmacokinetic properties of several novel oligonucleotide analogs in mice.
Crooke, S. T., Graham, M. J., Zuckerman, J. E., Brooks, D., Conklin, B., Cummins, L. L., Greig, M. J., Guinosso, C. J., Kornbrust, D. J., Manoharan, M., et al. (1996) · Journal of Pharmacology and Experimental Therapeutics · doi:10.1016/s0022-3565(25)12951-0 · Cited by 227 · see citing works
Matsuda, S., Keiser, K., Nair, J. K., Charissé, K., Manoharan, R. M., Kretschmer, P., Peng, C. G., Kel’in, A. V., Pachamuthu, K., Willoughby, J. L. S., et al. (2015) · ACS Chemical Biology · doi:10.1021/cb501028c · Cited by 222 · see citing works
A Novel Mechanism Is Involved in Cationic Lipid-Mediated Functional siRNA Delivery
Lu, J. J.-Q., Langer, R., & Chen, J. (2009) · Molecular Pharmaceutics · doi:10.1021/mp900023v · Cited by 219 · see citing works
Hau, P., Jachimczak, P., Schlingensiepen, R., Schulmeyer, F. J., Jauch, T., Steinbrecher, A., Brawanski, A. T., Proescholdt, M. A., Schlaier, J. R., Buchroithner, J., et al. (2007) · Oligonucleotides · doi:10.1089/oli.2006.0053 · Cited by 215 · see citing works
KRIEG, A. M., Tonkinson, J. L., Matson, S., Zhao, Q., Saxon, M. E., Zhang, L. M., Bhanja, U., Yakubov, L. A., & Stein, C. (1993) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.90.3.1048 · Cited by 201 · see citing works
Receptor-mediated endocytosis: The intracellular journey of transferrin and its receptor
Dautry‐Varsat, A. (1986) · Biochimie · doi:10.1016/s0300-9084(86)80004-9 · Cited by 199 · see citing works
EGF induces macropinocytosis and SNX1-modulated recycling of E-cadherin
Bryant, D. M., Kerr, M. C., Hammond, L. A., Joseph, S. R., Mostov, K. E., Teasdale, R. D., & Stow, J. L. (2007) · Journal of Cell Science · doi:10.1242/jcs.000653 · Cited by 185 · see citing works
Rajeev, K. G., Nair, J. K., Jayaraman, M., Charissé, K., Taneja, N., O′Shea, J., Willoughby, J. L. S., Yucius, K., Nguyen, T. M., Shulga‐Morskaya, S., et al. (2015) · ChemBioChem · doi:10.1002/cbic.201500023 · Cited by 185 · see citing works
Sequential signaling in plasma-membrane domains during macropinosome formation in macrophages
Yoshida, S., Hoppe, A. D., Araki, N., & Swanson, J. A. (2009) · Journal of Cell Science · doi:10.1242/jcs.053207 · Cited by 168 · see citing works
Madshus, I. H., & Stang, E. (2009) · Journal of Cell Science · doi:10.1242/jcs.050260 · Cited by 167 · see citing works
Biochemical analysis of distinct Rab5- and Rab11-positive endosomes along the transferrin pathway
Trischler, M., Stoorvogel, W., & Ullrich, O. (1999) · Journal of Cell Science · doi:10.1242/jcs.112.24.4773 · Cited by 162 · see citing works
Imaging individual microRNAs in single mammalian cells in situ
Lü, J., & Tsourkas, A. (2009) · Nucleic Acids Research · doi:10.1093/nar/gkp482 · Cited by 158 · see citing works
Hydrophobically Modified siRNAs Silence Huntingtin mRNA in Primary Neurons and Mouse Brain
Alterman, J. F., Hall, L. M., Coles, A. H., Hassler, M. R., Didiot, M.-C., Chase, K., Abraham, J., Sottosanti, E., Johnson, E., Sapp, E., et al. (2015) · Molecular Therapy - Nucleic Acids · doi:10.1038/mtna.2015.38 · Cited by 158 · see citing works
Self-citationCao, T. T., Mays, R. W., & von Zastrow, M. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.38.24592 · Cited by 138 · see citing works
Grewal, P. K. (2010) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/s0076-6879(10)79013-3 · Cited by 136 · see citing works
Transferrin receptor recycling in the absence of perinuclear recycling endosomes
Sheff, D. R., Pelletier, L., O’Connell, C. B., Warren, G., & Mellman, I. (2002) · The Journal of Cell Biology · doi:10.1083/jcb.20111048 · Cited by 133 · see citing works
Eyster, C. A., Cole, N. B., Harrington, S. P., Viswanathan, K., Früh, K., & Donaldson, J. G. (2011) · Molecular Biology of the Cell · doi:10.1091/mbc.e10-11-0874 · Cited by 130 · see citing works
DNA/RNA heteroduplex oligonucleotide for highly efficient gene silencing
Nishina, K., Piao, W., Yoshida‐Tanaka, K., Sujino, Y., Nishina, T., Yamamoto, T., Nitta, K., Yoshioka, K., Kuwahara, H., Yasuhara, H., et al. (2015) · Nature Communications · doi:10.1038/ncomms8969 · Cited by 127 · see citing works
Leonard, D. M., Hayakawa, A., Lawe, D., Lambright, D., Bellve, K., Standley, C., Lifshitz, L. M., Fogarty, K., & Corvera, S. (2008) · Journal of Cell Science · doi:10.1242/jcs.031484 · Cited by 123 · see citing works
Self-citationChen, Y., Deng, L., Maeno-Hikichi, Y., Lai, M., Chang, S., Chen, G., & Zhang, J.-F. (2003) · Cell · doi:10.1016/s0092-8674(03)00726-8 · Cited by 122 · see citing works
Dynamics of Tubulovesicular Recycling Endosomes in Hippocampal Neurons
Prekeris, R., Foletti, D. L., & Scheller, R. H. (1999) · Journal of Neuroscience · doi:10.1523/jneurosci.19-23-10324.1999 · Cited by 109 · see citing works
Hopkins, C. R., Miller, K., & Beardmore, J. M. (1985) · Journal of Cell Science · doi:10.1242/jcs.1985.supplement_3.17 · Cited by 104 · see citing works
Petrova, N. S., Chernikov, I. V., Meschaninova, M. I., Dovydenko, I. S., Venyaminova, A. G., Аркадьевна, З. М., Vlassov, V. V., & Chernolovskaya, E. L. (2011) · Nucleic Acids Research · doi:10.1093/nar/gkr1002 · Cited by 96 · see citing works
Rabenosyn-5 defines the fate of the transferrin receptor following clathrin-mediated endocytosis
Navaroli, D. M., Bellve, K., Standley, C., Lifshitz, L. M., Cardia, J., Lambright, D., Leonard, D. M., Fogarty, K., & Corvera, S. (2012) · Proceedings of the National Academy of Sciences of the United States of America · doi:10.1073/pnas.1115495109 · Cited by 93 · see citing works
Self-citationQuantitative evaluation of siRNA delivery in vivo
Pei, Y., Hancock, P. J., Zhang, H., Bartz, R., Cherrin, C., Innocent, N., Pomerantz, C. J., Seitzer, J. L., Koser, M. L., Abrams, M. T., et al. (2010) · RNA · doi:10.1261/rna.2255810 · Cited by 82 · see citing works
Byrne, M., Tzekov, R. T., Wang, Y., Rodgers, A., Cardia, J. P., Ford, G., Holton, K., Pandarinathan, L., Lapierre, J., Stanney, W., et al. (2013) · Journal of Ocular Pharmacology and Therapeutics · doi:10.1089/jop.2013.0148 · Cited by 78 · see citing works
Self-citationPlasma Membrane Domains Specialized for Clathrin-mediated Endocytosis in Primary Cells*
Bellve, K., Leonard, D. M., Standley, C., Lifshitz, L. M., Tuft, R., Hayakawa, A., Corvera, S., & Fogarty, K. (2006) · Journal of Biological Chemistry · doi:10.1074/jbc.m511370200 · Cited by 74 · see citing works
Self-citationRegarding the Amazing Choreography of Clathrin Coats
Traub, L. M. (2011) · PLoS Biology · doi:10.1371/journal.pbio.1001037 · Cited by 73 · see citing works
Kuwahara, H., Nishina, K., Yoshida, K., Nishina, T., Yamamoto, M., Saito, Y., Piao, W., Yoshida, M., Mizusawa, H., & Yokota, T. (2011) · Molecular Therapy · doi:10.1038/mt.2011.186 · Cited by 71 · see citing works
Mipomersen and other therapies for the treatment of severe familial hypercholesterolemia
Burnett, J., Bell, D. A., Hooper, & Watts, G. F. (2012) · Vascular Health and Risk Management · doi:10.2147/vhrm.s28581 · Cited by 66 · see citing works
Cyclodextrin-Containing Polymers: Versatile Platforms of Drug Delivery Materials
Heidel, J. D., & Schluep, T. (2012) · Journal of Drug Delivery · doi:10.1155/2012/262731 · Cited by 65 · see citing works
Hayakawa, A., Leonard, D. M., Murphy, S. T., Hayes, S. J., Soto, M. C., Fogarty, K., Standley, C., Bellve, K., Lambright, D., Mello, C., et al. (2006) · Proceedings of the National Academy of Sciences of the United States of America · doi:10.1073/pnas.0508832103 · Cited by 58 · see citing works
Self-citationVideira, M., Arranja, A., Rafael, D. F. D. S., & Gaspar, R. S. (2013) · Nanomedicine Nanotechnology Biology and Medicine · doi:10.1016/j.nano.2013.11.018 · Cited by 58 · see citing works
GLUT4 and Transferrin Receptor Are Differentially Sorted Along the Endocytic Pathway in CHO Cells
Wei, M. L., Bonzelius, F., Scully, R., Kelly, R. B., & Herman, G. A. (1998) · The Journal of Cell Biology · doi:10.1083/jcb.140.3.565 · Cited by 58 · see citing works
Analysis of synaptic vesicle endocytosis in synaptosomes by high-content screening
Daniel, J. A., Malladi, C. S., Kettle, E., McCluskey, A., & Robinson, P. J. (2012) · Nature Protocols · doi:10.1038/nprot.2012.070 · Cited by 57 · see citing works
ARAP1 Regulates Endocytosis of EGFR
Yoon, H., Lee, J., & Randazzo, P. A. (2008) · Traffic · doi:10.1111/j.1600-0854.2008.00839.x · Cited by 52 · see citing works
cAMP-sensitive endocytic trafficking in A6 epithelia
Butterworth, M. B., Helman, S. I., & Els, W. J. (2001) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.2001.280.4.c752 · Cited by 52 · see citing works
Rahajeng, J., Caplan, S., & Naslavsky, N. (2009) · Experimental Cell Research · doi:10.1016/j.yexcr.2009.11.007 · Cited by 51 · see citing works
Beas, A. O., Taupin, V., Teodorof‐Diedrich, C., Nguyen, L. T., Garcia‐Marcos, M., & Farquhar, M. G. (2012) · Molecular Biology of the Cell · doi:10.1091/mbc.e12-02-0133 · Cited by 48 · see citing works
Effect of cycloheximide on epidermal growth factor receptor trafficking and signaling
Oksvold, M. P., Pedersen, N. M., Forfang, L., & Smeland, E. B. (2012) · FEBS Letters · doi:10.1016/j.febslet.2012.08.022 · Cited by 29 · see citing works
Felber, A. E., Bayó‐Puxán, N., Deleavey, G. F., Castagner, B., Damha, M. J., & Leroux, J. (2012) · Biomaterials · doi:10.1016/j.biomaterials.2012.05.019 · Cited by 20 · see citing works
Dye selection for live cell imaging of intact siRNA
Hirsch, M., Strand, D. J., & Helm, M. (2011) · Biological Chemistry · doi:10.1515/bc-2011-256 · Cited by 13 · see citing works
Flagged first, then by citations
1 of the 83 references couldn’t be matched to a known paper.
Research works printed but not linked: 1
- [61]Crooke S.T. Graham M.J. Zuckerman J.E. Conklin B.S. Griffey H. Pharmacokinetic Analogs in mice J. Pharmacol. Exp. Ther. 1996 277 923 937 8627575
Cited by
Sorted by citations
The current state and future directions of RNAi-based therapeutics
Setten, R. L., Rossi, J. J., & Han, S.-P. (2019) · Nature Reviews Drug Discovery · doi:10.1038/s41573-019-0017-4 · Cited by 1,443 · see citing works
Detection of immunogenic cell death and its relevance for cancer therapy
Fucikova, J., Kepp, O., Kasikova, L., Petroni, G., Yamazaki, T., Liu, P., Zhao, L., Spisek, R., Kroemer, G., & Galluzzi, L. (2020) · Cell Death & Disease · doi:10.1038/s41419-020-03221-2 · Cited by 1,138 · see citing works
Translation and functional roles of circular RNAs in human cancer
Lei, M., Zheng, G., Ning, Q., Zheng, J., & Dong, D. (2020) · Molecular Cancer · doi:10.1186/s12943-020-1135-7 · Cited by 814 · see citing works
Chemistry, mechanism and clinical status of antisense oligonucleotides and duplex RNAs
Shen, X., & Corey, D. R. (2017) · Nucleic Acids Research · doi:10.1093/nar/gkx1239 · Cited by 639 · see citing works
Emerging role of tumor-related functional peptides encoded by lncRNA and circRNA
Wu, P., Mo, Y., Miao, P., Tang, T. F., Zhong, Y. M., Deng, X., Xiong, F., Guo, C. J., Wu, X., Li, Y., et al. (2020) · Molecular Cancer · doi:10.1186/s12943-020-1147-3 · Cited by 533 · see citing works
Gong, C., Tian, J., Wang, Z., Gao, Y., Wu, X., Ding, X., Qiang, L., Li, G., Han, Z., Yuan, Y., et al. (2019) · Journal of Nanobiotechnology · doi:10.1186/s12951-019-0526-7 · Cited by 339 · see citing works
Changes in m6A RNA Methylation Contribute to Heart Failure Progression by Modulating Translation
Berulava, T., Buchholz, E., Vakhtang, E., Peña, T., Islam, R., Lbik, D., Mohamed, B. A., Renner, A., von Lewinski, D., Sacherer, M., et al. (2019) · European Journal of Heart Failure · doi:10.1002/ejhf.1672 · Cited by 326 · see citing works
Investigating the pharmacodynamic durability of GalNAc–siRNA conjugates
Brown, C. R., Gupta, S., Qin, J., Racie, T., He, G., Lentini, S., Malone, R., Yu, M., Matsuda, S., Shulga-Morskaya, S., et al. (2020) · Nucleic Acids Research · doi:10.1093/nar/gkaa670 · Cited by 285 · see citing works
Alterman, J. F., Godinho, B. M. D. C., Hassler, M. R., Ferguson, C. M., Echeverria, D., Sapp, E., Haraszti, R. A., Coles, A. H., Conroy, F., Miller, R., et al. (2019) · Nature Biotechnology · doi:10.1038/s41587-019-0205-0 · Cited by 227 · see citing works
Self-citationCurrent Development of siRNA Bioconjugates: From Research to the Clinic
Chernikov, I. V., Vlassov, V. V., & Chernolovskaya, E. L. (2019) · Frontiers in Pharmacology · doi:10.3389/fphar.2019.00444 · Cited by 216 · see citing works
RNAi modulation of placental sFLT1 for the treatment of preeclampsia
Turanov, A. A., Lo, A. S., Hassler, M. R., Makris, A., Ashar-Patel, A., Alterman, J. F., Coles, A. H., Haraszti, R. Á., Roux, L., Godinho, B. M. D. C., et al. (2018) · Nature Biotechnology · doi:10.1038/nbt.4297 · Cited by 212 · see citing works
Self-citationDiverse lipid conjugates for functional extra-hepatic siRNA delivery in vivo
Biscans, A., Coles, A. H., Haraszti, R. Á., Echeverria, D., Hassler, M. R., Osborn, M. F., & Khvorova, A. (2018) · Nucleic Acids Research · doi:10.1093/nar/gky1239 · Cited by 206 · see citing works
Self-citationvon Herrmann, K. M., Salas, L. A., Martinez, E. M., Young, A. L., Howard, J. M., Feldman, M. S., Christensen, B. C., Wilkins, O. M., Lee, S., Hickey, W. F., et al. (2018) · npj Parkinson s Disease · doi:10.1038/s41531-018-0061-5 · Cited by 185 · see citing works
The new function of circRNA: translation
Shi, Y., Jia, X., & Xu, J. (2020) · Clinical & Translational Oncology · doi:10.1007/s12094-020-02371-1 · Cited by 182 · see citing works
Comparison of partially and fully chemically-modified siRNA in conjugate-mediated delivery in vivo
Hassler, M. R., Turanov, A. A., Alterman, J. F., Haraszti, R. A., Coles, A. H., Osborn, M. F., Echeverria, D., Nikan, M., Salomon, W. E., Roux, L., et al. (2018) · Nucleic Acids Research · doi:10.1093/nar/gky037 · Cited by 181 · see citing works
Self-citationEditorial focus: understanding off-target effects as the key to successful RNAi therapy
Bartoszewski, R., & Sikorski, A. F. (2019) · Cellular & Molecular Biology Letters · doi:10.1186/s11658-019-0196-3 · Cited by 150 · see citing works
The regulation of protein translation and its implications for cancer
Song, P., Yang, F., Jin, H., & Wang, X. (2021) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-020-00444-9 · Cited by 144 · see citing works
Improving siRNA Delivery <i>In Vivo</i> Through Lipid Conjugation
Osborn, M. F., & Khvorova, A. (2018) · Nucleic Acid Therapeutics · doi:10.1089/nat.2018.0725 · Cited by 137 · see citing works
Self-citation5΄-Vinylphosphonate improves tissue accumulation and efficacy of conjugated siRNAs in vivo
Haraszti, R. Á., Roux, L., Coles, A. H., Turanov, A. A., Alterman, J. F., Echeverria, D., Godinho, B. M. D. C., D, N. A. M., & Khvorova, A. (2017) · Nucleic Acids Research · doi:10.1093/nar/gkx507 · Cited by 128 · see citing works
Self-citationIntracellular Trafficking and Endosomal Release of Oligonucleotides: What We Know and What We Don’t
Juliano, R. L. (2018) · Nucleic Acid Therapeutics · doi:10.1089/nat.2018.0727 · Cited by 122 · see citing works
Inhibition of the CDK2 and Cyclin A complex leads to autophagic degradation of CDK2 in cancer cells
Zhang, J., Gan, Y., Li, H., Yin, J., He, X., Lin, L., Xu, S., Fang, Z., Kim, B.-W., Gao, L., et al. (2022) · Nature Communications · doi:10.1038/s41467-022-30264-0 · Cited by 119 · see citing works
Optimized Cholesterol-siRNA Chemistry Improves Productive Loading onto Extracellular Vesicles
Haraszti, R. Á., Miller, R., Didiot, M.-C., Biscans, A., Alterman, J. F., Hassler, M. R., Roux, L., Echeverria, D., Sapp, E., DiFiglia, M., et al. (2018) · Molecular Therapy · doi:10.1016/j.ymthe.2018.05.024 · Cited by 106 · see citing works
Self-citationCurrent Aspects of siRNA Bioconjugate for In Vitro and In Vivo Delivery
Tai, W. (2019) · Molecules · doi:10.3390/molecules24122211 · Cited by 97 · see citing works
Wu, S., Zhang, A., Li, S., Chatterjee, S., Qi, R., Segura-Ibarra, V., Mauro Ferrari, B., Gupte, A. A., Blanco, E., & Hamilton, D. J. (2018) · Advanced Science · doi:10.1002/advs.201700530 · Cited by 94 · see citing works
A molecular network of conserved factors keeps ribosomes dormant in the egg
Leesch, F., Lorenzo‐Orts, L., Pribitzer, C., Grishkovskaya, I., Roehsner, J., Chugunova, A. A., Matzinger, M., Roitinger, E., Belačić, K., Kandolf, S., et al. (2023) · Nature · doi:10.1038/s41586-022-05623-y · Cited by 89 · see citing works
Malik, N., Yan, H., Moshkovich, N., Palangat, M., Yang, H. H., Sanchez, V. C., Cai, Z., Peat, T. J., Jiang, S., Liu, C., et al. (2019) · Nature Communications · doi:10.1038/s41467-019-10102-6 · Cited by 81 · see citing works
Biscans, A., Caiazzi, J., Davis, S. M., McHugh, N., Sousa, J., & Khvorova, A. (2020) · Nucleic Acids Research · doi:10.1093/nar/gkaa595 · Cited by 68 · see citing works
Self-citationMechanisms of palmitic acid-conjugated antisense oligonucleotide distribution in mice
Chappell, A. E., Gaus, H. J., Berdeja, A., Gupta, R., Jo, M., Prakash, T. P., Oestergaard, M. E., Swayze, E. E., & Seth, P. P. (2020) · Nucleic Acids Research · doi:10.1093/nar/gkaa164 · Cited by 73 · see citing works
Shmushkovich, T., Monopoli, K. R., Homsy, D., Leyfer, D., Betancur-Boissel, M., Khvorova, A., & Wolfson, A. D. (2018) · Nucleic Acids Research · doi:10.1093/nar/gky745 · Cited by 72 · see citing works
Self-citationNon-viral nanoparticles for RNA interference: Principles of design and practical guidelines
Liu, Z., Wang, S., Tapeinos, C., Torrieri, G., Känkänen, V., El‐Sayed, N., Python, A., Hirvonen, J. J., & Santos, H. A. (2021) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2021.05.018 · Cited by 70 · see citing works
Biscans, A., Coles, A. H., Echeverria, D., & Khvorova, A. (2019) · Journal of Controlled Release · doi:10.1016/j.jconrel.2019.03.028 · Cited by 64 · see citing works
Self-citationChemical optimization of siRNA for safe and efficient silencing of placental sFLT1
Davis, S. M., Hariharan, V. N., Lo, A. S., Turanov, A. A., Echeverria, D., Sousa, J., McHugh, N., Biscans, A., Alterman, J. F., Karumanchi, S. A., et al. (2022) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2022.06.009 · Cited by 59 · see citing works
Self-citationSegal, M., Biscans, A., Gilles, M.-E., Anastasiadou, E., Luca, R. D., Lim, J., Khvorova, A., & Slack, F. J. (2019) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2019.11.008 · Cited by 58 · see citing works
Self-citationALKBH3-dependent m1A demethylation of Aurora A mRNA inhibits ciliogenesis
Kuang, W., Jin, H., Yang, F., Chen, X., Liu, J., Li, T., Chang, Y., Liu, M., Xu, Z., Huo, C., et al. (2022) · Cell Discovery · doi:10.1038/s41421-022-00385-3 · Cited by 57 · see citing works
Biscans, A., Haraszti, R. Á., Echeverria, D., Miller, R., Didiot, M.-C., Nikan, M., Roux, L., D, N. A. M., & Khvorova, A. (2018) · Molecular Therapy · doi:10.1016/j.ymthe.2018.03.019 · Cited by 55 · see citing works
Self-citationLigtenberg, M. A., de Coaña, Y. P., Shmushkovich, T., Yoshimoto, Y., Truxová, I., Yang, Y., Betancur-Boissel, M., Eliseev, A. V., Wolfson, A. D., & Kiessling, R. W. (2018) · Molecular Therapy · doi:10.1016/j.ymthe.2018.04.015 · Cited by 53 · see citing works
Godinho, B. M. D. C., Gilbert, J. W., Haraszti, R. A., Coles, A. H., Biscans, A., Roux, L., Nikan, M., Echeverria, D., Hassler, M., & Khvorova, A. (2017) · Nucleic Acid Therapeutics · doi:10.1089/nat.2017.0690 · Cited by 52 · see citing works
Self-citationModulating intracellular pathways to improve non-viral delivery of RNA therapeutics
de Vyver, T. V., Smedt, S. C. D., & Raemdonck, K. (2021) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2021.114041 · Cited by 51 · see citing works
Conroy, F., Miller, R., Alterman, J. F., Hassler, M. R., Echeverria, D., Godinho, B. M. D. C., Knox, E. G., Sapp, E., Sousa, J., Yamada, K., et al. (2022) · Nature Communications · doi:10.1038/s41467-022-33061-x · Cited by 50 · see citing works
Self-citationOptimized delivery of siRNA into 3D tumor spheroid cultures in situ
Morgan, R. G., Chambers, A. C., Legge, D. N., Coles, S. J., Greenhough, A., & Williams, A. C. (2018) · Scientific Reports · doi:10.1038/s41598-018-26253-3 · Cited by 47 · see citing works
Liu, W., Iwamoto, N., Marappan, S., Luu, K., Tripathi, S., Purcell-Estabrook, E., Shelke, J. D., Shah, H., Lamattina, A., Pan, Q., et al. (2023) · Nucleic Acids Research · doi:10.1093/nar/gkad268 · Cited by 44 · see citing works
Chemical modulation of siRNA lipophilicity for efficient delivery
Tai, W. (2019) · Journal of Controlled Release · doi:10.1016/j.jconrel.2019.06.022 · Cited by 44 · see citing works
Shen, P., Reineke, L. C., Knutsen, E., Chen, M., Pichler, M., Ling, H. Y., & Călin, G. A. (2018) · Molecular Oncology · doi:10.1002/1878-0261.12384 · Cited by 42 · see citing works
Massaro, C., Sgueglia, G., Frattolillo, V., Baglio, S. R., Altucci, L., & Dell’Aversana, C. (2020) · Pharmaceutics · doi:10.3390/pharmaceutics12100980 · Cited by 43 · see citing works
Ly, S., Echeverria, D., Sousa, J., & Khvorova, A. (2020) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2020.07.029 · Cited by 38 · see citing works
Self-citationTranslation regulation in response to stress
Williams, T. D., & Rousseau, A. (2024) · The FEBS Journal · doi:10.1111/febs.17076 · Cited by 34 · see citing works
The ice age – A review on formulation of Adeno-associated virus therapeutics
Grossen, P., Skaripa-Koukelli, I., van Haasteren, J., Machado, A. H. E., & Dürr, C. (2023) · European Journal of Pharmaceutics and Biopharmaceutics · doi:10.1016/j.ejpb.2023.07.002 · Cited by 36 · see citing works
Lacroix, A., Fakih, H. H., & Sleiman, H. F. (2020) · Journal of Controlled Release · doi:10.1016/j.jconrel.2020.04.020 · Cited by 37 · see citing works
Noncovalent tagging of siRNA with steroids for transmembrane delivery
Tai, W., & Gao, X. (2018) · Biomaterials · doi:10.1016/j.biomaterials.2018.02.007 · Cited by 34 · see citing works
Cell Type Impacts Accessibility of mRNA to Silencing by RNA Interference
Ferguson, C. M., Echeverria, D., Hassler, M. R., Ly, S., & Khvorova, A. (2020) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2020.06.006 · Cited by 33 · see citing works
Self-citationJuliano, R. L. (2021) · Biomedicines · doi:10.3390/biomedicines9050512 · Cited by 33 · see citing works
Fàbrega, C., Aviñó, A. M., Navarro, N., Jorge, A. F., Grijalvo, S., & Eritja, R. (2023) · Pharmaceutics · doi:10.3390/pharmaceutics15020320 · Cited by 33 · see citing works
Cellular Targeting of Oligonucleotides by Conjugation with Small Molecules
Hawner, M., & Ducho, C. (2020) · Molecules · doi:10.3390/molecules25245963 · Cited by 25 · see citing works
PUS7‐dependent pseudouridylation of ALKBH3 mRNA inhibits gastric cancer progression
Chang, Y., Jin, H., Cui, Y., Yang, F., Chen, K., Kuang, W., Huo, C., Xu, Z., Li, Y., Lin, A., et al. (2024) · Clinical and Translational Medicine · doi:10.1002/ctm2.1811 · Cited by 30 · see citing works
Cyclic peptide-based nanostructures as efficient siRNA carriers
Panigrahi, B., Singh, R. K., Mishra, S., & Mandal, D. (2018) · Artificial Cells Nanomedicine and Biotechnology · doi:10.1080/21691401.2018.1511574 · Cited by 31 · see citing works
RNAi-based modulation of IFN-γ signaling in skin
Tang, Q., Sousa, J., Echeverria, D., Fan, X., Hsueh, Y.-C., Afshari, K., MeHugh, N., Cooper, D., Vangjeli, L., Monopoli, K. R., et al. (2022) · Molecular Therapy · doi:10.1016/j.ymthe.2022.04.019 · Cited by 31 · see citing works
Self-citationModulation of PDCD1 exon 3 splicing
Sun, J., Bai, J., Jiang, T., Gao, Y., & Hua, Y. (2019) · RNA Biology · doi:10.1080/15476286.2019.1659080 · Cited by 30 · see citing works
Rpl12 is a conserved ribophagy receptor
Chen, Y., Hu, J., Zhao, P., Fang, J., Kuang, Y., Liu, Z., Dong, S., Yao, W., Ding, Y., Wang, X., et al. (2025) · Nature Cell Biology · doi:10.1038/s41556-024-01598-2 · Cited by 29 · see citing works
Zhao, L., Wang, L., Zhang, C., Liu, Z., Piao, Y., Yan, J., Xiang, R., Yao, Y., & Shi, Y. (2019) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-019-0060-y · Cited by 29 · see citing works
Monty, M. A., Islam, M. A., Xu, N., Tan, J., Tuhin, I. J., Tang, X., Miao, M., Wu, D., & Yu, L. (2021) · British Journal of Pharmacology · doi:10.1111/bph.15414 · Cited by 28 · see citing works
Nucleic acid amphiphiles: Synthesis, properties, and applications
Gubu, A., Zhang, X., Lü, A., Zhang, B., Ma, Y., & Zhang, G. (2023) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2023.05.022 · Cited by 28 · see citing works
Monopoli, K. R., Korkin, D., & Khvorova, A. (2023) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2023.06.010 · Cited by 27 · see citing works
Self-citationInsight into the siRNA transmembrane delivery—From cholesterol conjugating to tagging
Zheng, Y., & Tai, W. (2019) · Wiley Interdisciplinary Reviews Nanomedicine and Nanobiotechnology · doi:10.1002/wnan.1606 · Cited by 27 · see citing works
Systematic analysis of siRNA and mRNA features impacting fully chemically modified siRNA efficacy
Davis, S. M., Hildebrand, S. R., MacMillan, H. J., Monopoli, K. R., Buchwald, J. E., Sousa, J., Cooper, D., Ly, S., Echeverria, D., McHugh, N., et al. (2025) · Nucleic Acids Research · doi:10.1093/nar/gkaf479 · Cited by 25 · see citing works
Self-citationMaguregui, A., & Abe, H. (2020) · ChemBioChem · doi:10.1002/cbic.202000009 · Cited by 24 · see citing works
Davis, S. M., Sousa, J., Vangjeli, L., Hassler, M. R., Echeverria, D., Knox, E. G., Turanov, A. A., Alterman, J. F., & Khvorova, A. (2020) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2020.05.010 · Cited by 23 · see citing works
Self-citationKB343, a Cyclic Tris-guanidine Alkaloid from Palauan ZoantharianEpizoanthus illoricatus
Matsumura, K., Taniguchi, T., Reimer, J. D., Noguchi, S., Fujita, M., & Sakai, R. (2018) · Organic Letters · doi:10.1021/acs.orglett.8b01069 · Cited by 22 · see citing works
Park, Y., Koga, Y., Su, C., Waterbury, A. L., Johnny, C. L., & Liau, B. B. (2019) · Angewandte Chemie International Edition · doi:10.1002/anie.201901386 · Cited by 22 · see citing works
Comprehensive discovery and functional characterization of the noncanonical proteome
Shi, C., Liu, F., Su, X., Yang, Z., Wang, Y., Xie, S., Xie, S., Sun, Q., Chen, Y., Sang, L., et al. (2025) · Cell Research · doi:10.1038/s41422-024-01059-3 · Cited by 21 · see citing works
Ohmer, M., Tzivelekidis, T., Niedenführ, N., Volceanov, L., Barth, S., Vier, J., Börries, M., Busch, H. S., Kook, L., Biniossek, M. L., et al. (2018) · Cellular Microbiology · doi:10.1111/cmi.12993 · Cited by 21 · see citing works
Loading of Extracellular Vesicles with Hydrophobically Modified siRNAs
Didiot, M.-C., Haraszti, R. Á., D, N. A. M., & Khvorova, A. (2018) · Methods in molecular biology · doi:10.1007/978-1-4939-7652-2_16 · Cited by 20 · see citing works
Self-citationTransport Oligonucleotides—A Novel System for Intracellular Delivery of Antisense Therapeutics
Markov, O. V., Filatov, A. V., Kupryushkin, M. S., Chernikov, I. V., Patutina, O., Strunov, A. A., Chernolovskaya, E. L., Vlassov, V. V., Pyshnyi, D. V., & Аркадьевна, З. М. (2020) · Molecules · doi:10.3390/molecules25163663 · Cited by 20 · see citing works
Yamada, K., Hildebrand, S. R., Davis, S. M., Miller, R., Conroy, F., Sapp, E., Caiazzi, J., Alterman, J. F., Roux, L., Echeverria, D., et al. (2021) · Nucleic Acids Research · doi:10.1093/nar/gkab1126 · Cited by 18 · see citing works
Self-citationBerk, C., Civenni, G., Wang, Y., Steuer, C., Catapano, C. V., & Hall, J. (2020) · Nucleic Acid Therapeutics · doi:10.1089/nat.2020.0852 · Cited by 19 · see citing works
Fluorocarbons Enhance Intracellular Delivery of Short STAT3-sensors and Enable Specific Imaging
Metelev, V., Zhang, S., Zheng, S., Kumar, A. T. N., & Bogdanov, A. A., Jr. (2017) · Theranostics · doi:10.7150/thno.19704 · Cited by 19 · see citing works
Calorie restriction reprograms diurnal rhythms in protein translation to regulate metabolism
Makwana, K., Gosai, N., Poe, A., & Kondratov, R. V. (2018) · The FASEB Journal · doi:10.1096/fj.201802167r · Cited by 18 · see citing works
Systemic Delivery of Small Interfering RNA Therapeutics: Obstacles and Advances
Chandela, A., & Ueno, Y. (2019) · Reviews in Agricultural Science · doi:10.7831/ras.7.10 · Cited by 18 · see citing works
Control of translation during the unfolded protein response in maize seedlings: Life without PERKs
Kanodia, P., Vijayapalani, P., Srivastava, R., Bi, R., Liu, P., Miller, W. A., & Howell, S. H. (2020) · Plant Direct · doi:10.1002/pld3.241 · Cited by 18 · see citing works
Cheng, S.-Y., Caiazzi, J., Biscans, A., Alterman, J. F., Echeverria, D., McHugh, N., Hassler, M. R., Jolly, S. M., Giguere, D., Cipi, J., et al. (2023) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2023.102088 · Cited by 19 · see citing works
Self-citationStrategies to Improve the Lipophilicity of Hydrophilic Macromolecular Drugs
Lindner, S., Keim, S., Haddadzadegan, S., Romero, O. F., Zöller, K., Stern, G., Cesi, I., Kafedjiiski, K., & Bernkop‐Schnürch, A. (2025) · Advanced Healthcare Materials · doi:10.1002/adhm.202503721 · Cited by 16 · see citing works
Chernikov, I. V., Gladkikh, D. V., Meschaninova, M. I., Karelina, U. A., Venyaminova, A. G., Аркадьевна, З. М., Vlassov, V. V., & Chernolovskaya, E. L. (2018) · Nucleic Acid Therapeutics · doi:10.1089/nat.2018.0745 · Cited by 16 · see citing works
Biomimetic Dextran–Peptide Vectors for Efficient and Safe siRNA Delivery
Qu, X., Hu, Y., Wang, H.-F., Song, H., Young, M. A., Xu, F., Liu, Y., & Cheng, G. (2019) · ACS Applied Bio Materials · doi:10.1021/acsabm.8b00714 · Cited by 15 · see citing works
Wang, Q., & Mao, Y. (2023) · Advanced Biotechnology · doi:10.1007/s44307-023-00006-4 · Cited by 14 · see citing works
Repositioning the antihistamine ebastine as an intracellular siRNA delivery enhancer
Muntean, C., Blondeel, E., Harinck, L., Pednekar, K. P., Prakash, J., Wever, O. D., Chain, J. L., Smedt, S. C. D., Remaut, K., & Raemdonck, K. (2023) · International Journal of Pharmaceutics · doi:10.1016/j.ijpharm.2023.123348 · Cited by 13 · see citing works
Hernández, R. B., Moteshareie, H., Burnside, D. J., McKay, B. C., & Golshani, A. (2019) · Scientific Reports · doi:10.1038/s41598-019-42907-2 · Cited by 13 · see citing works
Aptamer-incorporated DNA Holliday junction for the targeted delivery of siRNA
Jeong, E. H., Jeong, H., Jang, B., Kim, B. Y., Kim, M., Kwon, H., Lee, K., & Lee, H. (2017) · Journal of Industrial and Engineering Chemistry · doi:10.1016/j.jiec.2017.07.025 · Cited by 12 · see citing works
Xue, M.-G., Cong, F., Zheng, W.-L., Xu, R.-Q., Liu, X., Bao, H., Sung, Y., Xi, Y.-M., He, F., Ma, J., et al. (2023) · Cell Death Discovery · doi:10.1038/s41420-023-01587-8 · Cited by 12 · see citing works
Cholesterol Conjugates of Small Interfering RNA: Linkers and Patterns of Modification
Chernikov, I. V., Ponomareva, U. A., Meschaninova, M. I., Bachkova, I. K., Vlassov, V. V., Аркадьевна, З. М., & Chernolovskaya, E. L. (2024) · Molecules · doi:10.3390/molecules29040786 · Cited by 11 · see citing works
Important Aspects of siRNA Design for Optimal Efficacy In Vitro and In Vivo
Bhakta-Yadav, M. S., & Brown, T. L. (2025) · International Journal of Cell Biology · doi:10.1155/ijcb/6663816 · Cited by 10 · see citing works
Akçaöz-Alasar, A., Tüncel, Ö., Sağlam, B., Gazaloğlu, Y., Atbinek, M., Cagiral, U., İşcan, E., Özhan, G., & Akgül, B. (2024) · Journal of Cellular Physiology · doi:10.1002/jcp.31176 · Cited by 10 · see citing works
Haraszti, R. Á., Coles, A. H., D, N. A. M., Khvorova, A., & Didiot, M.-C. (2017) · BIO-PROTOCOL · doi:10.21769/bioprotoc.2338 · Cited by 10 · see citing works
Self-citationIdentification of selective and non-selective C9ORF72 targeting in vivo active siRNAs
Gilbert, J. W., Kennedy, Z., Godinho, B. M. D. C., Summers, A., Weiss, A., Echeverria, D., Bramato, B. M., McHugh, N., Cooper, D., Yamada, K., et al. (2024) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2024.102291 · Cited by 9 · see citing works
Self-citationMethods for Evaluating the Stimuli-Responsive Delivery of Nucleic Acid and Gene Medicines
Fumoto, S., & Nishida, K. (2017) · Chemical and Pharmaceutical Bulletin · doi:10.1248/cpb.c17-00096 · Cited by 9 · see citing works
Alterman, J. F., Coles, A. H., Hall, L. M., D, N. A. M., Khvorova, A., & Didiot, M.-C. (2017) · BIO-PROTOCOL · doi:10.21769/bioprotoc.2501 · Cited by 9 · see citing works
Self-citationRules and impacts of nonsense‐mediated mRNA decay in the degradation of long noncoding RNAs
Singh, A. K. (2024) · Wiley Interdisciplinary Reviews - RNA · doi:10.1002/wrna.1853 · Cited by 8 · see citing works
The Medicinal Chemistry of Antisense Oligonucleotides
Watts, J. K. (2018) · Wiley eBooks · doi:10.1002/9781119070153.ch2 · Cited by 8 · see citing works
Cuiffo, B., Maxwell, M., Yan, D., Guemiri, R., Boone, A., Bellet, D., Rivest, B., Cardia, J. P., Robert, C., & Fricker, S. P. (2024) · Frontiers in Immunology · doi:10.3389/fimmu.2024.1501679 · Cited by 7 · see citing works
Self-citationChemical Modifications in Nucleic Acid Therapeutics
Lennox, K. A., Young, R. C., & Behlke, M. A. (2025) · Methods in molecular biology · doi:10.1007/978-1-0716-4742-4_3 · Cited by 7 · see citing works
Kajino, R., Sakamoto, S., & Ueno, Y. (2022) · RSC Advances · doi:10.1039/d2ra00705c · Cited by 7 · see citing works
Maxwell, M., Yan, D., Rivest, B., Boone, A., Cardia, J. P., & Noeßner, E. (2024) · Cancer Immunology Immunotherapy · doi:10.1007/s00262-024-03835-x · Cited by 7 · see citing works
Self-citation
- Authors
- Ly, S., Navaroli, D. M., Didiot, M.-C., Cardia, J., Pandarinathan, L., Alterman, J. F., Fogarty, K., Standley, C., Lifshitz, L. M., Bellve, K. D., Prot, M., Echeverria, D., Corvera, S., & Khvorova, A.
- Affiliations
- University of Massachusetts Chan Medical School (US)
- Phio Pharmaceuticals (United States) (US)
- Corresponding author
- Khvorova, Anastasia
- ORCID
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2016-11-28
- Volume
- 45
- Issue
- 1
- Pages
- 15-25
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- PMC
- PMC5224471
- OpenAlex
- W2557242880
- Cited by
- 132
- References
- 83
- Citations by year
- 2026: 4 · 2025: 13 · 2024: 13 · 2023: 14 · 2022: 9 · 2021: 9 · 2020: 19 · 2019: 22 · 2018: 21 · 2017: 8
- FWCI
- 8.84
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Nucleic Acids Research (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- The Journal of the American Medical Association (JAMA) Network (American Medical Association) (submittedVersion, cc-by)
- HighWire Press Open Archive (submittedVersion)
- Funders
- University of Massachusetts (DK32520)
- University of Oxford
- National Institutes of Health (1s10od020012-01)
- National Institute of General Medical Sciences (R01GM108803, R25GM113686)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Keywords
- siRNA delivery, cellular uptake, endocytosis, early endosomes, transferrin receptor, EEA1, membrane association
- MeSH terms
- Animals, Biological Transport, Chlorocebus aethiops, Cholesterol, Endocytosis, Epidermal Growth Factor, HeLa Cells, Humans, Microscopy, Fluorescence, Endosomes, Transferrin, Gene Expression, COS Cells, Cyclophilins, Vesicular Transport Proteins, RNA, Small Interfering, Hydrophobic and Hydrophilic Interactions
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
