The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Astrocyte Ca 2+ waves trigger responses in microglial cells in brain slices
Abstract
Pathologic impacts in the brain lead to a widespread activation of microglial cells far beyond the site of injury. Here, we demonstrate that glial Ca 2+ waves can trigger responses in microglial cells. We elicited Ca 2+ waves in corpus callosum glial cells by electrical stimulation or local adenosine triphosphate (ATP) ejection in acute brain slices. Macroglial cells, but not microglia, were bulk‐loaded with Ca 2+ ‐sensitive dyes. Using a transgenic animal in which astrocytes were labeled by the enhanced green fluorescence protein (EGFP) allowed us to identify the reacting cell populations: the wave activated a Ca 2+ response in both astrocytes and non‐astrocytic glial cells and spread over hundreds of micrometers even into the adjacent cortical and ventricular cell layers. Regenerative ATP release and subsequent activation of metabotropic purinergic receptors caused the propagation of the glial Ca 2+ wave: the wave was blocked by the purinergic receptor antagonist Reactive Blue 2 and was not affected by the gap junction blocker octanol, but enhanced in Ca 2+ free saline. To test whether microglial cells respond to the wave, microglial cells were labeled with a dye‐coupled lectin and membrane currents were recorded with the patch‐clamp technique. When the wave passed by, a current with the characteristics of a purinergic response was activated. Thus, Ca 2+ waves in situ are not restricted to astrocytic cells, but broadly activate different glial cell types.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Glutamate Induces Calcium Waves in Cultured Astrocytes: Long-Range Glial Signaling
Cornell-Bell, A. H., Finkbeiner, S. M., Cooper, M. S., & Smith, S. J. (1990) · Science · doi:10.1126/science.1967852 · Cited by 1,846
Cotrina, M. L., Lin, J. H.-C., López-Garcı́a, J. C., Naus, C. C. G., & Nedergaard, M. (2000) · The Journal of Neuroscience · doi:10.1523/jneurosci.20-08-02835.2000 · Cited by 388
Electrophysiological properties of microglial cells in normal and pathologic rat brain slices
Boucsein, C., Kettenmann, H., & Nolte, C. (2000) · European Journal of Neuroscience · doi:10.1046/j.1460-9568.2000.00100.x · Cited by 166
Self-citation
Cited by
ATP mediates rapid microglial response to local brain injury in vivo
Davalos, D., Grutzendler, J., Yang, G., Kim, J. V., Zuo, Y., Jung, S., Littman, D. R., Dustin, M. L., & Gan, W.-B. (2005) · Nature Neuroscience · doi:10.1038/nn1472 · Cited by 3,913
Molecular Physiology of P2X Receptors
North, R. A. (2002) · Physiological Reviews · doi:10.1152/physrev.00015.2002 · Cited by 2,919
New roles for astrocytes: redefining the functional architecture of the brain.
Nedergaard, M., Ransom, B., & Goldman, S. A. (2003) · Trends in Neurosciences · doi:10.1016/j.tins.2003.08.008 · Cited by 1,334
Astrocytes contain a vesicular compartment that is competent for regulated exocytosis of glutamate
Bezzi, P., Gundersen, V., Galbete, J. L., Seifert, G., Steinhäuser, C., Pilati, E., & Volterra, A. (2004) · Nature Neuroscience · doi:10.1038/nn1246 · Cited by 671
Heterogeneity of astrocytic form and function.
Oberheim, N. A., Goldman, S. A., & Nedergaard, M. (2012) · Methods in Molecular Biology · doi:10.1007/978-1-61779-452-0_3 · Cited by 638
Astrocyte-Derived ATP Induces Vesicle Shedding and IL-1β Release from Microglia
Bianco, F., Pravettoni, E., Colombo, A., Schenk, U., Möller, T., Matteoli, M., & Verderio, C. (2005) · The Journal of Immunology · doi:10.4049/jimmunol.174.11.7268 · Cited by 596
Yiangou, Y., Facer, P., Durrenberger, P., Chessell, I. P., Naylor, A., Bountra, C., Banati, R. R., & Anand, P. (2006) · BMC Neurology · doi:10.1186/1471-2377-6-12 · Cited by 509
Liu, W., Tang, Y., & Feng, J. (2011) · Life Sciences · doi:10.1016/j.lfs.2011.05.011 · Cited by 303
Boucsein, C., Zacharias, R., Färber, K., Pavlovic, S., Hanisch, U., & Kettenmann, H. (2003) · European Journal of Neuroscience · doi:10.1046/j.1460-9568.2003.02663.x · Cited by 203
Self-citationCalcium signaling and gliotransmission in normal vs. reactive astrocytes
Agulhon, C., Sun, M.-Y., Murphy, T., Myers, T., Lauderdale, K., & Fiacco, T. A. (2012) · Frontiers in Pharmacology · doi:10.3389/fphar.2012.00139 · Cited by 167
Ballistic labeling and dynamic imaging of astrocytes in organotypic hippocampal slice cultures.
Benediktsson, A. M., Schachtele, S. J., Green, S. H., & Dailey, M. E. (2005) · Journal of Neuroscience Methods · doi:10.1016/j.jneumeth.2004.05.013 · Cited by 166
Astrocyte–neuron interactions in neurological disorders
Ricci, G., Volpi, L., Pasquali, L., Petrozzi, L., & Siciliano, G. (2009) · Journal of Biological Physics · doi:10.1007/s10867-009-9157-9 · Cited by 155
Glial Connexins and Pannexins in the Healthy and Diseased Brain
Giaume, C., Naus, C. C., Sáez, J. C., & Leybaert, L. (2021) · Physiological Reviews · doi:10.1152/physrev.00043.2018 · Cited by 154
Heterogeneity of Astrocytes in Grey and White Matter
Köhler, S., Winkler, U., & Hirrlinger, J. (2021) · Neurochemical Research · doi:10.1007/s11064-019-02926-x · Cited by 123
Receptors, Ion Channels, and Signaling Mechanisms Underlying Microglial Dynamics
Madry, C., & Attwell, D. (2015) · Journal of Biological Chemistry · doi:10.1074/jbc.r115.637157 · Cited by 109
“Astrocyte Ca(2) waves can be sensed by some microglia via purinergic receptors that generate an outward membrane current (55).”
BackgroundUpregulation of α-synuclein following immune activation: Possible trigger of Parkinson's disease
Kasen, A., Houck, C., Burmeister, A. R., Sha, Q., Brundin, L., & Brundin, P. (2022) · Neurobiology of Disease · doi:10.1016/j.nbd.2022.105654 · Cited by 78
Astrocyte Function is Modified by Alzheimer's Disease-like Pathology in Aged Mice
Peters, O., Schipke, C. G., Philipps, A., Haas, B., Pannasch, U., Wang, L. P., Benedetti, B., Kingston, A. E., & Kettenmann, H. (2009) · Journal of Alzheimer's Disease · doi:10.3233/jad-2009-1140 · Cited by 76
Self-citationWendt, S., Maricos, M., Vana, N., Meyer, N., Guneykaya, D., Semtner, M., & Kettenmann, H. (2017) · Neurobiology of Aging · doi:10.1016/j.neurobiolaging.2017.05.027 · Cited by 69
Self-citationInflammatory Pathways in Parkinson’s Disease; A BNE Microarray Study
Durrenberger, P. F., Grünblatt, E., Fernando, F. S., Monoranu, C. M., Evans, J., Riederer, P., Reynolds, R., & Dexter, D. T. (2012) · Parkinson's Disease · doi:10.1155/2012/214714 · Cited by 63
Biological wires, communication systems, and implications for disease
Friesen, D. E., Craddock, T. J. A., Kalra, A. P., & Tuszynski, J. A. (2015) · Biosystems · doi:10.1016/j.biosystems.2014.10.006 · Cited by 48
Philtjens, S., Turnbull, M. T., Thedy, B. P., Moon, Y., & Kim, J. (2020) · doi:10.1101/2020.04.27.064881 · Cited by 7
Metadata
- Authors
- Schipke, C. G., Boucsein, C., Ohlemeyer, C., Kirchhoff, F., & Kettenmann, H.
- Venue
- The FASEB Journal
- Publisher
- Wiley
- Published
- 2001-12-28
- Volume
- 16
- Issue
- 2
- Pages
- 1-16
- ISSN
- 0892-6638 (ISSN-L)
- 1530-6860
- OpenAlex
- W1597609025
- Cited by
- 252
- References
- 3
- FWCI
- 7.49
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
