Amyloid β-peptide levels in laser capture microdissected cornu ammonis 1 pyramidal neurons of Alzheimer's brain
Abstract
Deposition of the amyloid beta-peptide (Abeta) is a pathophysiological event associated with Alzheimer's disease. Although much is known about the molecular composition of extracellular Abeta deposits, the role of the intracellular pool of Abeta is not fully understood. We investigated whether Abeta levels are increased in cornu ammonis 1 pyramidal neurons of Alzheimer's disease hippocampus, using laser capture microdissection to isolate the neurons and enzyme-linked immunosorbent assay for quantification. Our results showed increased Abeta42 levels and an elevated Abeta42/Abeta40 ratio in neurons from sporadic as well as from familial cases of Alzheimer's disease, whereas Abeta40 levels remain unchanged between the cases and controls. We speculate that intracellular accumulation of Abeta42 increase vulnerability of cornu ammonis 1 pyramidal neurons in Alzheimer's disease.
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Intraneuronal Aβ accumulation has been found in the brains of patients presenting AD or mild cognitive impairment and is suggested to represent an early step in AD neuropathology.
“Intraneuronal Aβ accumulation has been found in the brains of patients presenting AD or mild cognitive impairment [3, 21, 41, 47, 78], and studies suggest that this phenomenon represents an early step in AD neuropathology [40].”
From Senescence-related impairment of autophagy induces toxic intraneuronal amyloid-β accumulation in a mouse model of amyloid pathology (2023) · cites this paper as [3]
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- Authors
- Aoki, M., Volkmann, I., Tjernberg, L. O., Winblad, B., & Bogdanovic, N.
- Affiliations
- Karolinska Institutet (SE)
- Venue
- NeuroReport
- Published
- 2008-07-16
- Volume
- 19
- Issue
- 11
- Pages
- 1085-1089
- ISSN
- 0959-4965 (ISSN-L)
- OpenAlex
- W1968092641
- Cited by
- 53
- References
- 29
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 4 · 2023: 3 · 2022: 1 · 2021: 5 · 2020: 1 · 2019: 1 · 2018: 2 · 2017: 1 · 2016: 2 · 2015: 2 · 2014: 2 · 2013: 1 · 2012: 9
- FWCI
- 3.61
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Neuroreport (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Dainippon Sumitomo Pharma
- Topics
- Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
- Health Sciences › Medicine › Psychiatry and Mental health › Dementia and Cognitive Impairment Research
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Keywords
- amyloid-beta, Alzheimer's disease, cornu ammonis 1, pyramidal neurons, hippocampus, amyloid-beta 42, Aβ42/Aβ40 ratio, familial Alzheimer's disease, sporadic Alzheimer's disease, neuronal vulnerability
- MeSH terms
- Aged, Aged, 80 and over, Alzheimer Disease, Brain, Cytoplasm, Enzyme-Linked Immunosorbent Assay, Female, Hippocampus, Humans, Lasers, Male, Peptide Fragments, Amyloid beta-Peptides, Pyramidal Cells, Microdissection
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