Cited by 53Alzheimer's disease research and treatmentsDementia and Cognitive Impairment ResearchNeuroinflammation and Neurodegeneration Mechanisms

Amyloid β-peptide levels in laser capture microdissected cornu ammonis 1 pyramidal neurons of Alzheimer's brain

Aoki, Mikio, Volkmann, Inga, Tjernberg, Lars O., Winblad, Bengt, Bogdanovic, Nenad·2008·NeuroReport

Abstract

Deposition of the amyloid beta-peptide (Abeta) is a pathophysiological event associated with Alzheimer's disease. Although much is known about the molecular composition of extracellular Abeta deposits, the role of the intracellular pool of Abeta is not fully understood. We investigated whether Abeta levels are increased in cornu ammonis 1 pyramidal neurons of Alzheimer's disease hippocampus, using laser capture microdissection to isolate the neurons and enzyme-linked immunosorbent assay for quantification. Our results showed increased Abeta42 levels and an elevated Abeta42/Abeta40 ratio in neurons from sporadic as well as from familial cases of Alzheimer's disease, whereas Abeta40 levels remain unchanged between the cases and controls. We speculate that intracellular accumulation of Abeta42 increase vulnerability of cornu ammonis 1 pyramidal neurons in Alzheimer's disease.

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Crossref · 10.1097/wnr.0b013e328302c858

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Authors
Aoki, M., Volkmann, I., Tjernberg, L. O., Winblad, B., & Bogdanovic, N.
Affiliations
Karolinska Institutet (SE)
Venue
NeuroReport
Published
2008-07-16
Volume
19
Issue
11
Pages
1085-1089
ISSN
0959-4965 (ISSN-L)
OpenAlex
W1968092641
Cited by
53
References
29
Citations by year
2026: 1 · 2025: 2 · 2024: 4 · 2023: 3 · 2022: 1 · 2021: 5 · 2020: 1 · 2019: 1 · 2018: 2 · 2017: 1 · 2016: 2 · 2015: 2 · 2014: 2 · 2013: 1 · 2012: 9
FWCI
3.61
Type
journal article
Language
en
Open access
closed
Locations
  • Neuroreport (publishedVersion)
  • PubMed (publishedVersion)
Funders
Dainippon Sumitomo Pharma
Topics
  • Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
  • Health Sciences › Medicine › Psychiatry and Mental health › Dementia and Cognitive Impairment Research
  • Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
Keywords
amyloid-beta, Alzheimer's disease, cornu ammonis 1, pyramidal neurons, hippocampus, amyloid-beta 42, Aβ42/Aβ40 ratio, familial Alzheimer's disease, sporadic Alzheimer's disease, neuronal vulnerability
MeSH terms
Aged, Aged, 80 and over, Alzheimer Disease, Brain, Cytoplasm, Enzyme-Linked Immunosorbent Assay, Female, Hippocampus, Humans, Lasers, Male, Peptide Fragments, Amyloid beta-Peptides, Pyramidal Cells, Microdissection

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