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PreprintCited by 61RNA and protein synthesis mechanismsRNA Research and SplicingAdvanced Proteomics Techniques and Applications

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The relationship of mRNA with protein expression in CD8 + T cells associates with gene class and gene characteristics

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Nicolet, Benoît P., Wolkers, Monika C.·2020·PLoS ONE
DOI 10.1101/2020.04.21.053884PMC PMC9581405

Preprint — published as 10.1371/journal.pone.0276294 · Paper page

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Abstract

SUMMARY: T cells are key players in our defence against infections and malignancies. When T cells differentiate or become activated, they undergo substantial alterations in gene expression. Even though RNA expression levels are now well documented throughout different stages of T cells, it is not well understood how mRNA expression translates into the protein landscape. By combining paired RNA sequencing and massspectrometry data of primary human CD8 + T cells, we report that mRNA expression is a poor proxy for the overall protein output, irrespective of the differentiation or activation status. Yet, gene class stratification revealed a function-specific correlation of mRNA with protein expression. This gene class-specific expression pattern associated with differences in gene characteristics such as sequence conservation and untranslated region (UTR) lengths. In addition, the presence of AU-rich elements in the 3’UTR associated with alterations in mRNA and protein abundance T cell activation dependent, gene class-specific manner. In conclusion, our study highlights the role of gene characteristics as a determinant for gene expression in T cells.

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Metadata

Authors
Nicolet, B. P., & Wolkers, M. C.
Affiliations
Sanquin (NL)
Corresponding author
Wolkers, Monika C.
ORCID
Venue
PLoS ONE
Publisher
openRxiv
Published
2020-04-22
Volume
17
Issue
10
Pages
e0276294-e0276294
ISSN
1932-6203 (ISSN-L)
DOI
10.1101/2020.04.21.053884
PMC
PMC9581405
OpenAlex
W4306832366
Cited by
61
References
1
Citations by year
2026: 10 · 2025: 23 · 2024: 12 · 2023: 12 · 2022: 1 · 2021: 2 · 2020: 1
FWCI
3.92
Type
journal article
Language
en
Open access
Licence
Locations
  • PLoS ONE (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Data Archiving and Networked Services (DANS) (submittedVersion, other-oa)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • Pure Amsterdam UMC (submittedVersion, cc-by)
Funders
  • Oncode Institute
  • European Research Council (817533)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
  • Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
Keywords
CD8+ T cells, mRNA-protein correlation, gene expression, T cell activation, T cell differentiation, AU-rich elements, 3' untranslated region, sequence conservation, protein abundance
MeSH terms
Humans, Lymphocyte Activation, RNA, Messenger, CD8-Positive T-Lymphocytes, 3' Untranslated Regions, AU Rich Elements

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