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PreprintCited by 10Single-cell and spatial transcriptomicsNeuroscience and Neuropharmacology ResearchNeurobiology and Insect Physiology Research

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Cellular-resolution gene expression mapping reveals organization in the head ganglia of the gastropod, Berghia stephanieae

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Ramirez, M. Desmond, Bui, Thi N., Katz, Paul S.·2023·The Journal of Comparative Neurology
DOI 10.1101/2023.06.22.546160PMC PMC11198006

Preprint — published as 10.1002/cne.25628 · Paper page

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Abstract

Gastropod molluscs such as Aplysia , Lymnaea , and Tritonia have been important for determining fundamental rules of motor control, learning, and memory because of their large, individually identifiable neurons. Yet for the vast majority of gastropod neurons, as well as glia, there are no established molecular markers, limiting the ability to establish brain-wide structure-function relations. Here we combine high-throughput, single-cell RNA sequencing (scRNAseq) with in-situ hybridization chain reaction (HCR) in the nudibranch Berghia stephanieae to identify and visualize the expression of markers for cell types. Broad neuronal classes were characterized by genes associated with neurotransmitters, like acetylcholine, glutamate, serotonin, and GABA, as well as neuropeptides. These classes were subdivided by other genes including transcriptional regulators and unannotated genes. Marker genes expressed by neurons and glia formed discrete, previously unrecognized regions within and between ganglia. This study provides the foundation for understanding the fundamental cellular organization of gastropod nervous systems.

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Metadata

Authors
Ramirez, M. D., Bui, T. N., & Katz, P. S.
Affiliations
University of Massachusetts Amherst (US)
Corresponding author
Ramirez, M. Desmond
ORCID
Venue
The Journal of Comparative Neurology
Publisher
openRxiv
Published
2023-06-26
Volume
532
Issue
6
Pages
e25628-e25628
ISSN
0021-9967 (ISSN-L)
DOI
10.1101/2023.06.22.546160
PMC
PMC11198006
OpenAlex
W4399473889
Cited by
10
References
1
Citations by year
2026: 3 · 2025: 2 · 2024: 4 · 2023: 1
FWCI
1.23
Type
journal article
Language
en
Open access
Licence
cc-by-nc
Locations
  • The Journal of Comparative Neurology (publishedVersion, cc-by-nc)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • National Science Foundation (1812017, 2227963)
  • Harvard University
  • National Institutes of Health (1u01ns123972-01)
  • University of Massachusetts Amherst
  • National Institute of Neurological Disorders and Stroke (U01NS108637, U01 NS123972, R01NS133654)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Single-cell and spatial transcriptomics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurobiology and Insect Physiology Research
Keywords
Berghia stephanieae, gastropod nervous system, neuronal cell types, single-cell RNA sequencing, neuropeptides, acetylcholine, glutamate, serotonin, GABA, glia, molecular markers, neuronal organization
MeSH terms
Animals, Head, Neurons, Gene Expression, Ganglia, Invertebrate, Gastropoda

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