Cited by 177Plant Molecular Biology ResearchLight effects on plantsPlant Parasitism and Resistance

Auxin Perception--Structural Insights

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Calderon-Villalobos, L. I., Tan, X., Zheng, N., Estelle, M.·2010·Cold Spring Harbor Perspectives in Biology

Abstract

The identity of the auxin receptor(s) and the mechanism of auxin perception has been a subject of intense interest since the discovery of auxin almost a century ago. The development of genetic approaches to the study of plant hormone signaling led to the discovery that auxin acts by promoting degradation of transcriptional repressors called Aux/IAA proteins. This process requires a ubiquitin protein ligase (E3) called SCF(TIR1) and related SCF complexes. Surprisingly, auxin works by directly binding to TIR1, the F-box protein subunit of this SCF. Structural studies demonstrate that auxin acts like a "molecular glue," to stabilize the interaction between TIR1 and the Aux/IAA substrate. These exciting results solve an old problem in plant biology and reveal new mechanisms for E3 regulation and hormone perception.

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Crossref · 10.1101/cshperspect.a005546

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  • Auxin fills a gap between the E3 ligase and the degron motif of its substrate, complementing a suboptimal protein-protein interface, rather than allosterically inducing conformational changes in TIR1.

    “Instead of allosterically inducing conformational changes in the F-box protein TIR1, auxin fills a gap between the E3 ligase and the degron motif of its substrate, complementing a suboptimal protein–protein interface.”
  • Auxin promotes degradation of a family of transcription repressors by enhancing their otherwise weak interactions with the SCFTIR1 ubiquitin ligase complex.

    “auxin promotes the degradation of a family of transcription repressors by enhancing their otherwise weak interactions with the SCFTIR1 ubiquitin ligase complex8.”

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  1. Jonsson A Encyclopaedia of plant physiology 1961 959 1006 Berlin: Springer

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Authors
Calderon-Villalobos, L. I., Tan, X., Zheng, N., & Estelle, M.
Affiliations
  • Brigham and Women's Hospital (US)
  • University of Washington (US)
  • University of California San Diego (US)
Corresponding author
Estelle, M.
Venue
Cold Spring Harbor Perspectives in Biology
Published
2010-05-26
Volume
2
Issue
7
Pages
a005546-a005546
ISSN
1943-0264 (ISSN-L)
OpenAlex
W2161783871
Cited by
177
References
93
Citations by year
2026: 4 · 2025: 11 · 2024: 6 · 2023: 8 · 2022: 7 · 2021: 14 · 2020: 9 · 2019: 11 · 2018: 9 · 2017: 11 · 2016: 7 · 2015: 13 · 2014: 18 · 2013: 17 · 2012: 16
Type
posted content
Language
en
Open access
Locations
  • Cold Spring Harbor Perspectives in Biology (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Institute of General Medical Sciences (R01GM043644)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Light effects on plants
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Parasitism and Resistance
Keywords
auxin perception, auxin receptor, TIR1, SCF ubiquitin ligase, Aux/IAA proteins, F-box protein, E3 ubiquitin ligase, transcriptional repression, plant hormone signaling
MeSH terms
Hydrolysis, Indoleacetic Acids, Signal Transduction

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