Dorsoventral patterning in the Drosophila central nervous system: the vnd homeobox gene specifies ventral column identity
Open accessAbstract
The Drosophila CNS develops from three columns of neuroectodermal cells along the dorsoventral (DV) axis: ventral, intermediate, and dorsal. In this and the accompanying paper, we investigate the role of two homeobox genes, vnd and ind , in establishing ventral and intermediate cell fates within the Drosophila CNS. During early neurogenesis, Vnd protein is restricted to ventral column neuroectoderm and neuroblasts; later it is detected in a complex pattern of neurons. We use molecular markers that distinguish ventral, intermediate, and dorsal column neuroectoderm and neuroblasts, and a cell lineage marker for selected neuroblasts, to show that loss of vnd transforms ventral into intermediate column identity and that specific ventral neuroblasts fail to form. Conversely, ectopic vnd produces an intermediate to ventral column transformation. Thus, vnd is necessary and sufficient to induce ventral fates and repress intermediate fates within the Drosophila CNS. Vertebrate homologs of vnd ( Nkx2.1 and 2.2 ) are similarly expressed in the ventral CNS, raising the possibility that DV patterning within the CNS is evolutionarily conserved.
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vnd is thought to promote neuroblast formation by proneural-dependent and proneural-independent pathways.
“Similarly, vnd is thought to promote neuroblast formation by proneural-dependent and proneural-independent pathways (Jimenez et. al. 1995;McDonald et al. 1998).”
From Dorsoventral patterning in the <i>Drosophila</i> central nervous system: the <i>intermediate neuroblasts defective</i> homeobox gene specifies intermediate column identity (1998) · cites this paper as McDonald et al. 1998
In “Vnd expression during neurogenesis” (page unavailable), the paper proposes that vnd acts with the proneural genes achaete and scute and says it has an additional role beyond activating their expression. It does not establish that this additional role is independent of proneural genes altogether; the passage also mentions lethal of scute in other ventral neuroblasts. (LLM, conf=0.82)
Experimental — accuracy not yet measuredvnd function is required to establish the ventral border of ind: in the absence of vnd, expression of ind is observed in the ventral-column neuroectoderm and neuroblasts.
“In this paper and in McDonald et al. (1998) we show that vnd function is required to establish the ventral border of ind: In the absence of vnd, expression of ind is observed in the ventral-column neuroectoderm and neuroblasts.”
From Dorsoventral patterning in the <i>Drosophila</i> central nervous system: the <i>intermediate neuroblasts defective</i> homeobox gene specifies intermediate column identity (1998) · cites this paper as McDonald et al. 1998
Ind protein-containing cells are immediately adjacent but nonoverlapping with Vnd-containing cells of the ventral column neuroectoderm.
“The Ind proteincontaining cells are immediately adjacent but nonoverlapping with Vnd-containing cells of the ventral column neuroectoderm (see McDonald et al. 1998).”
From Dorsoventral patterning in the <i>Drosophila</i> central nervous system: the <i>intermediate neuroblasts defective</i> homeobox gene specifies intermediate column identity (1998) · cites this paper as McDonald et al. 1998
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Cites
Weiss, J. B., Von Ohlen, T., Mellerick, D. M., Dressler, G., Doe, C. Q., & Scott, M. P. (1998) · Genes & Development · doi:10.1101/gad.12.22.3591 · Cited by 250 · cited 3× · see citing works
“We use two intermediate column markers: Ind and Huckebein (Chu-LaGraff et al. 1995; McDonald and Doe 1997; Weiss et al. 1998).”
Self-citationmsh may play a conserved role in dorsoventral patterning of the neuroectoderm and mesoderm
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Self-citationAnti-Neural-Inhibition: A Conserved Mechanism for Neural Induction
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Weiss, J. B., Von Ohlen, T., Mellerick, D. M., Dressler, G., Doe, C. Q., & Scott, M. P. (1998) · Genes & Development · doi:10.1101/gad.12.22.3591 · Cited by 250 · see citing works
“In this paper and in McDonald et al. (1998) we show that vnd function is required to establish the ventral border of ind: In the absence of vnd, expression of ind is observed in the ventral-column neuroectoderm and neuroblasts.”
BackgroundMethodInfluentialSelf-citationBuescher, M., Hing, F. S., & Chia, W. (2002) · Development · doi:10.1242/dev.129.18.4193 · Cited by 133 · see citing works
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C. Hayward, D., Catmull, J., Reece-Hoyes, J. S., Berghammer, H., Dodd, H., Hann, S. J., Miller, D. J., & Ball, E. E. (2001) · Development Genes and Evolution · doi:10.1007/s004270000112 · Cited by 79 · see citing works
- Authors
- McDonald, J. A., Holbrook, S., Isshiki, T., Weiss, J., Doe, C. Q., & Mellerick, D. M.
- Affiliations
- Howard Hughes Medical Institute (US)
- Venue
- Genes & Development
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 1998-11-15
- Volume
- 12
- Issue
- 22
- Pages
- 3603-3612
- ISSN
- 0890-9369 (ISSN-L)
- 1549-5477
- PMC
- PMC317246
- OpenAlex
- W2109597876
- Cited by
- 162
- References
- 4
- Citations by year
- 2026: 6 · 2025: 5 · 2024: 1 · 2023: 2 · 2022: 6 · 2021: 3 · 2020: 4 · 2019: 5 · 2018: 2 · 2017: 4 · 2016: 4 · 2015: 7 · 2014: 1 · 2013: 9 · 2012: 1
- Type
- posted content
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Genes & Development (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Science Foundation
- National Institutes of Health
- University of Illinois at Urbana-Champaign
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurobiology and Insect Physiology Research
- Keywords
- dorsoventral patterning, Drosophila central nervous system, neuroectoderm, neuroblasts, cell fate specification, TTF-1, Nkx2.2
- MeSH terms
- Animals, Central Nervous System, Drosophila, Genes, Homeobox, Immunohistochemistry, Neurons, Transcription Factors, Homeodomain Proteins, Gene Expression Regulation, Developmental, Body Patterning, Drosophila Proteins
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