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LEDGF/p75 functions downstream from preintegration complex formation to effect gene-specific HIV-1 integration
Open accessAbstract
LEDGF/p75 directly interacts with lentiviral integrase proteins and can modulate their enzymatic activities and chromosomal association. A novel genetic knockout model was established that allowed us for the first time to analyze HIV-1 integration in the absence of LEDGF/p75 protein. Supporting a crucial role for the cofactor in viral replication, HIV-1 vector integration and reporter gene expression were significantly reduced in LEDGF-null cells. Yet, integrase processed the viral cDNA termini normally and maintained its local target DNA sequence preference during integration. Preintegration complexes extracted from knockout cells moreover supported normal levels of DNA strand transfer activity in vitro. In contrast, HIV-1 lost its strong bias toward integrating into transcription units, displaying instead increased affinity for promoter regions and CpG islands. Our results reveal LEDGF/p75 as a critical targeting factor, commandeering lentiviruses from promoter- and/or CpG island-proximal pathways that are favored by other members of Retroviridae . Akin to yeast retrotransposons, disrupting the lentiviral targeting mechanism significantly perturbs overall integration.
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“The genic proviruses that did form under these conditions uncharacteristically congregated around transcription start sites87,99–101.”
From Structure and function of retroviral integrase (2022) · cites this paper as [99]
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“Knockout of the PSIP1 gene, which encodes for LEDGF/p75, similarly suppressed levels of vDNA integration in cells several fold98,99.”
From Structure and function of retroviral integrase (2022) · cites this paper as [99]
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“Alterations that disrupted LEDGF/p75 binding to chromatin or IN ablated its cofactor function during HIV-1 infection99,103,112.”
From Structure and function of retroviral integrase (2022) · cites this paper as [99]
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“However, while LEDGF/p75 is critical, it is not strictly essential for HIV-1 integration and replication. This assertion is supported by the fact that HIV-1 was able to replicate, albeit at a much-reduced level, in LEDGF knockdown cells, and the replication could be restored upon expression of LEDGF/p75 (Llano et al. 2006, Shun et al. 2007).”
From Beyond reverse transcription: molecular mechanisms and emerging paradigms in retroviral replication (2026) · cites this paper as Shun et al. 2007
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HIV-1 Integration in the Human Genome Favors Active Genes and Local Hotspots
Schröder, A. R. W., Shinn, P., Chen, H., Berry, C., Ecker, J. R., & Bushman, F. (2002) · Cell · doi:10.1016/s0092-8674(02)00864-4 · Cited by 1,832
Transcription Start Regions in the Human Genome Are Favored Targets for MLV Integration
Wu, X., Li, Y., Crise, B., & Burgess, S. M. (2003) · Science · doi:10.1126/science.1083413 · Cited by 1,305
Retroviral DNA Integration: ASLV, HIV, and MLV Show Distinct Target Site Preferences
Mitchell, R., Beitzel, B. F., Schröder, A. R. W., Shinn, P., Chen, H., Berry, C. C., Ecker, J. R., & Bushman, F. D. (2004) · PLoS Biology · doi:10.1371/journal.pbio.0020234 · Cited by 1,002
Deininger, P. L., & Batzer, M. A. (1999) · Molecular Genetics and Metabolism · doi:10.1006/mgme.1999.2864 · Cited by 911
Inducible gene targeting in mice using the Cre/lox system.
Sauer, B. (1998) · Methods · doi:10.1006/meth.1998.0593 · Cited by 858
HIV-1 Integrase Forms Stable Tetramers and Associates with LEDGF/p75 Protein in Human Cells
Cherepanov, P., Maertens, G., Proost, P., Devreese, B., Van Beeumen, J., Engelborghs, Y., De Clercq, E., & Debyser, Z. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m209278200 · Cited by 704
Self-citationA role for LEDGF/p75 in targeting HIV DNA integration
Ciuffi, A., Llano, M., Poeschla, E., Hoffmann, C., Leipzig, J., Shinn, P., Ecker, J. R., & Bushman, F. (2005) · Nature Medicine · doi:10.1038/nm1329 · Cited by 658
HIV-1 DNA integration: Mechanism of viral DNA cleavage and DNA strand transfer
Engelman, A., Mizuuchi, K., & Craigie, R. (1991) · Cell · doi:10.1016/0092-8674(91)90297-c · Cited by 655
Self-citationAn essential role for LEDGF/p75 in HIV integration
Llano, M., Saenz, D. T., Meehan, A., Wongthida, P., Peretz, M., Walker, W. H., Teo, W., & Poeschla, E. M. (2006) · Science · doi:10.1126/science.1132319 · Cited by 537
Breaking barriers to transcription elongation
Saunders, A., Core, L. J., & Lis, J. T. (2006) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm1981 · Cited by 525
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Yan, N., Regalado-Magdos, A. D., Stiggelbout, B., Lee‐Kirsch, M. A., & Lieberman, J. (2010) · Nature Immunology · doi:10.1038/ni.1941 · Cited by 522
Christ, F., Voet, A., Marchand, A., Nicolet, S., Desimmie, B. A., Marchand, D., Bardiot, D., Van der Veken, N. J., Van Remoortel, B., Strelkov, S. V., et al. (2010) · Nature Chemical Biology · doi:10.1038/nchembio.370 · Cited by 479
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Lauberth, S. M., Nakayama, T., Wu, X., Ferris, A. L., Tang, Z., Hughes, S. H., & Roeder, R. G. (2013) · Cell · doi:10.1016/j.cell.2013.01.052 · Cited by 473
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Bushman, F. D., Malani, N., Fernandes, J. D., D’Orso, I., Cagney, G., Diamond, T. L., Zhou, H., Hazuda, D. J., Espeseth, A. S., König, R., et al. (2009) · PLoS Pathogens · doi:10.1371/journal.ppat.1000437 · Cited by 464
Pradeepa, M. M., Sutherland, H. G. E., Ule, J., Grimes, G. R., & Bickmore, W. A. (2012) · PLoS Genetics · doi:10.1371/journal.pgen.1002717 · Cited by 365
Nuclear architecture dictates HIV-1 integration site selection
Marini, B., Kertész‐Farkas, A., Ali, H., Lucic, B., Lisek, K., Manganaro, L., Pongor, S., Luzzati, R., Recchia, A., Mavilio, F., et al. (2015) · Nature · doi:10.1038/nature14226 · Cited by 360
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Lint, C. M. V., Bouchat, S., & Marcello, A. (2013) · Retrovirology · doi:10.1186/1742-4690-10-67 · Cited by 352
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Gillet, N. A., Malani, N., Melamed, A., Gormley, N. A., Carter, R., Bentley, D., Berry, C. C., Bushman, F. D., Taylor, G. P., & Bangham, C. R. M. (2011) · Blood · doi:10.1182/blood-2010-10-312926 · Cited by 332
Understanding HIV-1 latency provides clues for the eradication of long-term reservoirs
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Matreyek, K. A., Yücel, S. S., Li, X., & Engelman, A. (2013) · PLoS Pathogens · doi:10.1371/journal.ppat.1003693 · Cited by 280
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Sowd, G. A., Serrao, E., Wang, H., Wang, W., Fadel, H. J., Poeschla, E. M., & Engelman, A. N. (2016) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1524213113 · Cited by 249
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Lesbats, P., Engelman, A., & Cherepanov, P. (2016) · Chemical Reviews · doi:10.1021/acs.chemrev.6b00125 · Cited by 250
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Gao, X., Hou, Y., Ebina, H., Levin, H. L., & Voytas, D. F. (2008) · Genome Research · doi:10.1101/gr.7146408 · Cited by 216
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Christ, F., Shaw, S. M., Demeulemeester, J., Desimmie, B. A., Marchand, A., Butler, S., Smets, W. J., Chaltin, P., Westby, M., Debyser, Z., et al. (2012) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.00717-12 · Cited by 176
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Gupta, S. S., Maetzig, T., Maertens, G. N., Sharif, A., Rothe, M., Weidner-Glunde, M., Galla, M., Schambach, A., Cherepanov, P., & Schulz, T. F. (2013) · Journal of Virology · doi:10.1128/jvi.01942-13 · Cited by 139
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Kim, S., Kim, N., Dong, B., Boren, D., Lee, S. A., Gupta, J. D., Gaughan, C., Klein, E. A., Lee, C. S., Silverman, R. H., et al. (2008) · Journal of Virology · doi:10.1128/jvi.01299-08 · Cited by 106
Felice, B., Cattoglio, C., Cittaro, D., Testa, A., Miccio, A., Ferrari, G., Luzi, L., Recchia, A., & Mavilio, F. (2009) · PLoS ONE · doi:10.1371/journal.pone.0004571 · Cited by 104
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Gupta, K., Brady, T., Dyer, B. M., Malani, N., Hwang, Y., Male, F., Nolte, R. T., Wang, L., Velthuisen, E., Jeffrey, J., et al. (2014) · Journal of Biological Chemistry · doi:10.1074/jbc.m114.551119 · Cited by 81
Interactions between Retroviruses and the Host Cell Genome
Poletti, V., & Mavilio, F. (2017) · Molecular Therapy — Methods & Clinical Development · doi:10.1016/j.omtm.2017.10.001 · Cited by 85
Shun, M.-C., Botbol, Y., Li, X., Di Nunzio, F., Daigle, J. E., Yan, N., Lieberman, J., Lavigne, M., & Engelman, A. (2008) · Journal of Virology · doi:10.1128/jvi.01561-08 · Cited by 78
Self-citationStudamire, B., & Goff, S. P. (2008) · Retrovirology · doi:10.1186/1742-4690-5-48 · Cited by 85
Multifaceted HIV integrase functionalities and therapeutic strategies for their inhibition
Engelman, A. (2019) · Journal of Biological Chemistry · doi:10.1074/jbc.rev119.006901 · Cited by 83
Self-citationRetroviral integration site selection
Desfarges, S., & Ciuffi, A. (2010) · Viruses · doi:10.3390/v2010111 · Cited by 78
p12 Tethers the Murine Leukemia Virus Pre-integration Complex to Mitotic Chromosomes
Elis, E., Ehrlich, M., Prizan-Ravid, A., Laham-Karam, N., & Bacharach, E. (2012) · PLoS Pathogens · doi:10.1371/journal.ppat.1003103 · Cited by 76
Multiple cellular proteins interact with LEDGF/p75 through a conserved unstructured consensus motif
Tesina, P., Čermáková, K., Hořejší, M., Procházková, K., Fábry, M., Sharma, S., Christ, F., Demeulemeester, J., Debyser, Z., Rijck, J. D., et al. (2015) · Nature Communications · doi:10.1038/ncomms8968 · Cited by 74
High-resolution profiling of the LEDGF/p75 chromatin interaction in the ENCODE region
Rijck, J. D., Bartholomeeusen, K., Ceulemans, H., Debyser, Z., & Gijsbers, R. (2010) · Nucleic Acids Research · doi:10.1093/nar/gkq410 · Cited by 80
The chromatin landscape at the HIV-1 provirus integration site determines viral expression
Vansant, G., Chen, H., Zorita, E. V., Trejbalová, K., Miklík, D., Filion, G. J. P., & Debyser, Z. (2020) · Nucleic Acids Research · doi:10.1093/nar/gkaa536 · Cited by 79
Integration Site Selection by Retroviral Vectors: Molecular Mechanism and Clinical Consequences
Daniel, R., & Smith, J. A. (2008) · Human Gene Therapy · doi:10.1089/hum.2007.148 · Cited by 74
Aiyer, S., Swapna, G. V. T., Malani, N., Aramini, J. M., Schneider, W. M., Plumb, M. R., Ghanem, M., Larue, R. C., Sharma, A., Studamire, B., et al. (2014) · Nucleic Acids Research · doi:10.1093/nar/gku175 · Cited by 72
Zheng, Y., Ao, Z., Wang, B., Jayappa, K. D., & Yao, X. (2011) · Journal of Biological Chemistry · doi:10.1074/jbc.m110.184739 · Cited by 72
Demeulemeester, J., De Rijck, J., Gijsbers, R., & Debyser, Z. (2015) · BioEssays · doi:10.1002/bies.201500051 · Cited by 71
Lesbats, P., Botbol, Y. M., Chevereau, G., Vaillant, C., Calmels, C., Arnéodo, A., Andréola, M., Lavigne, M., & Parissi, V. (2011) · PLoS Pathogens · doi:10.1371/journal.ppat.1001280 · Cited by 87
Silvers, R. M., Smith, J. A., Schowalter, M. K., Litwin, S., Liang, Z.-H., Geary, K. R., & Daniel, R. (2009) · Human Gene Therapy · doi:10.1089/hum.2009.134 · Cited by 75
Gammaretroviral Integration into Nucleosomal Target DNA In Vivo
Roth, S. L., Malani, N., & Bushman, F. D. (2011) · Journal of Virology · doi:10.1128/jvi.00635-11 · Cited by 69
Fadel, H. J., Morrison, J. H., Saenz, D. T., Fuchs, J. R., Kvaratskhelia, M., Ekker, S. C., & Poeschla, E. M. (2014) · Journal of Virology · doi:10.1128/jvi.01397-14 · Cited by 70
Structural Basis for Inhibitor-Induced Aggregation of HIV Integrase
Gupta, K., Turkki, V., Sherrill-Mix, S., Hwang, Y., Eilers, G., Taylor, L., McDanal, C., Wang, P., Temelkoff, D., Nolte, R. T., et al. (2016) · PLOS Biology · doi:10.1371/journal.pbio.1002584 · Cited by 68
Li, X., Ewis, H. E., Hice, R. H., Malani, N., Parker, N., Zhou, L., Feschotte, C., Bushman, F. D., Atkinson, P. W., & Craig, N. L. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1121543109 · Cited by 69
Use of HIV as a gene transfer vector.
Pluta, K. D., & Kacprzak, M. M. (2009) · Acta Biochimica Polonica · doi:10.18388/abp.2009_2490 · Cited by 76
LEDGF/p75 Proteins with Alternative Chromatin Tethers Are Functional HIV-1 Cofactors
Meehan, A. M., Saenz, D. T., Morrison, J. H., Garcia-Rivera, J. A., Peretz, M., Llano, M., & Poeschla, E. M. (2009) · PLoS Pathogens · doi:10.1371/journal.ppat.1000522 · Cited by 71
HIV DNA is heavily uracilated, which protects it from autointegration
Yan, N., O’Day, E. M., Wheeler, L. A., Engelman, A., & Lieberman, J. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1102943108 · Cited by 70
Self-citation
Metadata
- Authors
- Shun, M.-C., Raghavendra, N. K., Vandegraaff, N., Daigle, J. E., Hughes, S., Kellam, P., Cherepanov, P., & Engelman, A.
- Affiliations
- Harvard University (US)
- Imperial College London (GB)
- University College London (GB)
- Venue
- Genes & Development
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2007-07-15
- Volume
- 21
- Issue
- 14
- Pages
- 1767-1778
- ISSN
- 0890-9369 (ISSN-L)
- 1549-5477
- PMC
- PMC1920171
- OpenAlex
- W2106494657
- Cited by
- 467
- References
- 61
- Citations by year
- 2026: 13 · 2025: 11 · 2024: 11 · 2023: 13 · 2022: 26 · 2021: 24 · 2020: 13 · 2019: 17 · 2018: 18 · 2017: 24 · 2016: 26 · 2015: 32 · 2014: 37 · 2013: 26 · 2012: 32
- Type
- posted content
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Genes & Development (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- UCL Discovery (University College London) (submittedVersion)
- Funders
- National Institutes of Health
- Medical Research Council
- National Institute of Allergy and Infectious Diseases (AI39394, R01AI039394, R37AI039394)
- Topics
- Life Sciences › Immunology and Microbiology › Virology › HIV Research and Treatment
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Virus-based gene therapy research
- Health Sciences › Medicine › Infectious Diseases › HIV/AIDS drug development and treatment
- Keywords
- LEDGF/p75, HIV-1 integration, preintegration complex, CpG islands, DNA strand transfer, retroviral replication
- MeSH terms
- Animals, Base Sequence, Binding Sites, Cell Line, DNA, Humans, Leukemia Virus, Murine, Promoter Regions, Genetic, Transcription Factors, HIV-1, Consensus Sequence, Virus Integration, Gene Deletion, CpG Islands, Integrases, Intercellular Signaling Peptides and Proteins, Adaptor Proteins, Signal Transducing, Mice, In Vitro Techniques
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