The DOI matches its Crossref record; 35 of the 35 references it cites were found in a registry or an index.
Chk1 and Claspin potentiate PCNA ubiquitination
Open accessAbstract
Chk1 is a kinase crucial for genomic integrity and an effector of ATR (ATM and Rad3-realated) in DNA damage response. Here, we show that Chk1 regulates the DNA damage-induced ubiquitination of proliferating cell nuclear antigen (PCNA), which facilitates the continuous replication of damaged DNA. Surprisingly, this Chk1 function requires the DNA replication protein Claspin but not ATR. Claspin, which is stabilized by Chk1, regulates the binding of the ubiquitin ligase Rad18 to chromatin. Timeless, a Claspin-associating protein, is also required for efficient PCNA ubiquitination. Thus, Chk1 and the Claspin–Timeless module of replication forks not only participate in ATR signaling, but also protect stressed forks independently of ATR.
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“activated Chk1 phosphorylates its own substrates such as protein Rad51, which is involved in the DSB repair by HR [193–195]”
From Functional interplay between ATM/ATR-mediated DNA damage response and DNA repair pathways in oxidative stress (2014) · cites this paper as [194]
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ATM and ATR Substrate Analysis Reveals Extensive Protein Networks Responsive to DNA Damage
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Global Mapping of the Yeast Genetic Interaction Network
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PCNA, the Maestro of the Replication Fork
Moldovan, G.-L., Pfander, B., & Jentsch, S. (2007) · Cell · doi:10.1016/j.cell.2007.05.003 · Cited by 1,796
ATR and ATRIP: Partners in Checkpoint Signaling
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Interaction of Human DNA Polymerase η with Monoubiquitinated PCNA
Kannouche, P. L., Wing, J., & Lehmann, A. R. (2004) · Molecular Cell · doi:10.1016/s1097-2765(04)00259-x · Cited by 845
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S-phase checkpoint proteins Tof1 and Mrc1 form a stable replication-pausing complex
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Regulation of monoubiquitinated PCNA by DUB autocleavage
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Cited by
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Y-family DNA polymerases and their role in tolerance of cellular DNA damage
Sale, J. E., Lehmann, A. R., & Woodgate, R. (2012) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm3289 · Cited by 665
Regulation of PCNA–protein interactions for genome stability
Mailand, N., Gibbs‐Seymour, I., & Bekker‐Jensen, S. (2013) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm3562 · Cited by 395
Forging Ahead through Darkness: PCNA, Still the Principal Conductor at the Replication Fork
Choe, K. N., & Moldovan, G.-L. (2017) · Molecular Cell · doi:10.1016/j.molcel.2016.12.020 · Cited by 325
Checkpoint kinase 1 in DNA damage response and cell cycle regulation
Patil, M., Pabla, N., & Dong, Z. (2013) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-013-1307-3 · Cited by 317
Ubiquitin signalling in DNA replication and repair
Ulrich, H. D., & Walden, H. (2010) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2921 · Cited by 280
Metadata
- Authors
- Yang, X. H., Shiotani, B., Classon, M., & Zou, L.
- Affiliations
- Harvard University (US)
- Venue
- Genes & Development
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2008-05-01
- Volume
- 22
- Issue
- 9
- Pages
- 1147-1152
- ISSN
- 0890-9369 (ISSN-L)
- 1549-5477
- PMC
- PMC2335311
- OpenAlex
- W2156915836
- Cited by
- 111
- References
- 35
- Citations by year
- 2025: 2 · 2024: 4 · 2023: 3 · 2022: 3 · 2021: 5 · 2020: 2 · 2019: 4 · 2018: 7 · 2017: 7 · 2016: 7 · 2015: 6 · 2014: 5 · 2013: 15 · 2012: 11
- Type
- posted content
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Genes & Development (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Susan G. Komen for the Cure
- Breast Cancer Alliance
- National Institutes of Health
- National Institute of General Medical Sciences (R01GM076388, GM076388)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ubiquitin and proteasome pathways
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Keywords
- Chk1, Claspin, PCNA ubiquitination, ATR signaling, DNA damage response, TIMELESS, replication fork, replication stress, genome stability
- MeSH terms
- Checkpoint Kinase 1, DNA Replication, HeLa Cells, Humans, Hydroxyurea, Morpholines, Plasmids, Protein Kinases, Pyrones, Transfection, Ultraviolet Rays, Blotting, Western, Protein Serine-Threonine Kinases, Cell Cycle Proteins, Proliferating Cell Nuclear Antigen, RNA, Small Interfering, Cell Line, Tumor, Adaptor Proteins, Signal Transducing, Ubiquitination, Ataxia Telangiectasia Mutated Proteins
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