Mismatch repair-dependent G2 checkpoint induced by low doses of SN1 type methylating agents requires the ATR kinase
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S N 1-type alkylating agents represent an important class of chemotherapeutics, but the molecular mechanisms underlying their cytotoxicity are unknown. Thus, although these substances modify predominantly purine nitrogen atoms, their toxicity appears to result from the processing of O6-methylguanine (6Me G)-containing mispairs by the mismatch repair (MMR) system, because cells with defective MMR are highly resistant to killing by these agents. In an attempt to understand the role of the MMR system in the molecular transactions underlying the toxicity of alkylating agents, we studied the response of human MMR-proficient and MMR-deficient cells to low concentrations of the prototypic methylating agent N-methyl-N′-nitro-N-nitrosoguanidine (MNNG). We now show that MNNG treatment induced a cell cycle arrest that was absolutely dependent on functional MMR. Unusually, the cells arrested only in the second G 2 phase after treatment. Downstream targets of both ATM (Ataxia telangiectasia mutated) and ATR (ATM and Rad3-related) kinases were modified, but only the ablation of ATR, or the inhibition of CHK1, attenuated the arrest. The checkpoint activation was accompanied by the formation of nuclear foci containing the signaling and repair proteins ATR, the S*/T*Q substrate, γ-H2AX, and replication protein A (RPA). The persistence of these foci implied that they may represent sites of irreparable damage.
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MLH1 is required for MNNG-induced ATR checkpoint activation, including Chk1 phosphorylation and colocalization of γ-H2AX and RPA.
“MLH1 is also required for MNNG-induced ATR checkpoint activation such as Chk1 phosphorylation and the colocalization of γ-H2AX and RPA [213]”
From Functional interplay between ATM/ATR-mediated DNA damage response and DNA repair pathways in oxidative stress (2014) · cites this paper as [213]
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- Authors
- Stojic, L., Mojas, N., Cejka, P., di Pietro, M., Ferrari, S., Marra, G., & Jiricny, J.
- Affiliations
- University of Zurich (CH)
- Venue
- Genes & Development
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2004-06-01
- Volume
- 18
- Issue
- 11
- Pages
- 1331-1344
- ISSN
- 0890-9369 (ISSN-L)
- 1549-5477
- PMC
- PMC420358
- OpenAlex
- W2127970987
- Cited by
- 246
- References
- 61
- Citations by year
- 2026: 6 · 2025: 4 · 2024: 8 · 2022: 5 · 2021: 4 · 2020: 6 · 2019: 7 · 2018: 3 · 2017: 7 · 2016: 7 · 2015: 7 · 2014: 17 · 2013: 14 · 2012: 14
- Type
- posted content
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- public-domain
- Locations
- Genes & Development (publishedVersion, public-domain)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Zurich Open Repository and Archive (University of Zurich) (acceptedVersion, other-oa)
- Zurich Open Repository and Archive (University of Zurich) (submittedVersion, other-oa)
- Zurich Open Repository and Archive (University of Zurich)
- Funders
- Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (064650)
- National Institutes of Health
- National Cancer Institute
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Health Sciences › Medicine › Pathology and Forensic Medicine › Genetic factors in colorectal cancer
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Chromatin Remodeling and Cancer
- Keywords
- DNA mismatch repair, G2 checkpoint, ATR kinase, N-methyl-N'-nitro-N-nitrosoguanidine, O6-methylguanine, cell cycle arrest, ATM kinase, Chk1, γH2AX, replication protein A, DNA repair
- MeSH terms
- Checkpoint Kinase 1, Caffeine, Cell Cycle, Cell Line, DNA, DNA Damage, DNA Repair, DNA-Binding Proteins, Dose-Response Relationship, Drug, Enzyme Inhibitors, Histones, Humans, Methylation, Methylnitronitrosoguanidine, Phosphorylation, Protein Kinases, Signal Transduction, G2 Phase, Protein Serine-Threonine Kinases, Cell Cycle Proteins, Staurosporine, Base Pair Mismatch, Tumor Suppressor Proteins, Protein Kinase Inhibitors, Replication Protein A, Ataxia Telangiectasia Mutated Proteins
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