The dorsal morphogen gradient regulates the mesoderm determinant twist in early Drosophila embryos.
Open accessAbstract
A gradient of the maternal morphogen dorsal (dl) initiates the differentiation of various tissues along the dorsal-ventral axis of early Drosophila embryos. dl is a sequence-specific DNA-binding protein that is related to the mammalian regulatory factor NF-kappa B. Previous studies suggest that dl can function as a transcriptional repressor. To determine how dl functions as an activator we have examined the promoter of the mesoderm determinant gene twist (twi). Genetic studies suggest that peak levels of dl protein in ventral regions of early embryos initiate twi expression. Using a combination of promoter fusion-P-transformation assays, and in vitro DNA-binding assays coupled with site-directed mutagenesis, we establish a direct link between dl-binding sites and twi expression in the early embryo. We also present evidence that the dorsal-ventral limits of twi expression depend on the number and affinity of dl-binding sites present in its promoter. A comparison of twi with a second dl target gene, zen, suggests a correlation between the affinities of dl-binding sites and response to different thresholds of dl morphogen.
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High levels of nuclear Dorsal present in ventral regions activate genes such as twist and snail, which are required for specification of the mesoderm.
“High levels of nuclear Dorsal present in ventral regions activate genes such as twist (twi) and snail (sna), which are required for specification of the mesoderm”
From Graded dorsal and differential gene regulation in the Drosophila embryo. (2009) · cites this paper as Jiang et al. 1991
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Research works printed but not linked: 2
- Kosman, D., Y.T. Ip, M. Levine, and K. Arora. 1991. The estab- lishment of the mesoderm-neuroectoderm boundary in the Drosophila embryo. Science (in press).
- Stephens, R., N. Rice, R. Hiebsch, H. Bose, and R. Gilden. 1983. Nucleotide sequence of v-re/: The oncogene of reticuloen- dotheliosis virus. Proc. Natl. Acad. Sci. 80: 6229-6233.
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- Authors
- Jiang, J., Kosman, D., Ip, Y. T., & Levine, M.
- Affiliations
- Columbia University (US)
- Venue
- Genes & Development
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 1991-10
- Volume
- 5
- Issue
- 10
- Pages
- 1881-1891
- ISSN
- 0890-9369 (ISSN-L)
- 1549-5477
- OpenAlex
- W1968993907
- Cited by
- 365
- References
- 0
- Citations by year
- 2026: 4 · 2025: 3 · 2024: 7 · 2023: 5 · 2022: 9 · 2021: 7 · 2020: 5 · 2019: 5 · 2018: 4 · 2017: 3 · 2016: 8 · 2015: 3 · 2014: 8 · 2013: 7 · 2012: 12
- Type
- posted content
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Genes & Development (publishedVersion)
- PubMed (publishedVersion)
- HighWire Press Open Archive (submittedVersion)
- Funders
- Life Sciences Research Foundation
- National Institutes of Health (GM 46638)
- National Institute of General Medical Sciences (GM-46638)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Wnt/β-catenin signaling in development and cancer
- Keywords
- twist gene, dorsal-ventral axis, transcriptional activation, DNA binding sites, promoter analysis, NF-κB, morphogen gradients, Drosophila embryo, transcriptional repressor
- MeSH terms
- Animals, Base Sequence, beta-Galactosidase, Deoxyribonuclease I, DNA, DNA Transposable Elements, DNA-Binding Proteins, Drosophila, Embryo, Nonmammalian, Feedback, Mesoderm, Molecular Sequence Data, Morphogenesis, Nuclear Proteins, Phosphoproteins, Promoter Regions, Genetic, Recombinant Fusion Proteins, Transcription Factors, Oligonucleotide Probes, Mutagenesis, Site-Directed, NF-kappa B, Drosophila Proteins
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