miRNPs: a novel class of ribonucleoproteins containing numerous microRNAs
Abstract
Gemin3 is a DEAD-box RNA helicase that binds to the Survival of Motor Neurons (SMN) protein and is a component of the SMN complex, which also comprises SMN, Gemin2, Gemin4, Gemin5, and Gemin6. Reduction in SMN protein results in Spinal muscular atrophy (SMA), a common neurodegenerative disease. The SMN complex has critical functions in the assembly/restructuring of diverse ribonucleoprotein (RNP) complexes. Here we report that Gemin3 and Gemin4 are also in a separate complex that contains eIF2C2, a member of the Argonaute protein family. This novel complex is a large ∼15S RNP that contains numerous microRNAs (miRNAs). We describe 40 miRNAs, a few of which are identical to recently described human miRNAs, a class of small endogenous RNAs. The genomic sequences predict that miRNAs are likely to be derived from larger precursors that have the capacity to form stem–loop structures.
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This study confirms the expression of previously cloned miRNAs.
“This study confirms the expression of previously cloned miRNAs [1-3,9,10]”
The panel of organs tested did not include all the organs and tissues that miRNAs were cloned from, and thus some organ-specific or organ-enriched miRNAs may be expressed in additional organs or tissues.
“The panel of organs that we tested in our study did not include all the organs and tissues that miRNAs were cloned from [1-3,9,10], and thus some organ-specific or organ-enriched miRNAs may be expressed in additional organs or tissues.”
The SMN protein is part of a large ribonucleoprotein complex that contains numerous miRNAs.
“This protein is part of a large ribonucleoprotein complex that contains numerous miRNAs (Mourelatos et al. 2002).”
From A microRNA array reveals extensive regulation of microRNAs during brain development (2003) · cites this paper as Mourelatos et al. 2002
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- Authors
- Mourelatos, Z., Dostie, J., Paushkin, S., Sharma, A., Charroux, B., Abel, L., Rappsilber, J., Mann, M., & Dreyfuss, G.
- Venue
- Genes & Development
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2002-03-15
- Volume
- 16
- Issue
- 6
- Pages
- 720-728
- ISSN
- 0890-9369
- 1549-5477
- Cited by
- 886
- References
- 1
- Language
- en
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