PreprintCited by 73Olfactory and Sensory Function StudiesMemory and Neural MechanismsNeurobiology and Insect Physiology Research

Isoproterenol Increases CREB Phosphorylation and Olfactory Nerve–Evoked Potentials in Normal and 5-HT-Depleted Olfactory Bulbs in Rat Pups Only at Doses That Produce Odor Preference Learning

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Yuan, Qi, Harley, Carolyn W., Bruce, Jamie C., Darby-King, Andrea, McLean, John H.·2000·Learning & Memory

Abstract

Norepinephrine (NE) and serotonin (5-HT) are important modulators of early odor preference learning. NE can act as an unconditioned stimulus (UCS), whereas 5-HT facilitates noradrenergic actions. In this study, we examined the phosphorylation of an important transcription factor, cAMP response element binding protein (CREB), which has been implicated in long-term-memory formation (McLean et al. 1999) during NE-induced odor preference learning in normal and olfactory bulb 5-HT-depleted rat pups. We also examined NE modulation of olfactory nerve-evoked field potentials (ON-EFPs) in anesthetized normal and bulbar 5-HT depleted pups. Systemic injection of 2 mg/kg isoproterenol (beta-adrenoceptor agonist) induced odor preference learning, enhanced pCREB expression in the olfactory bulbs at 10 min after odor pairing, and increased ON-EFPs in normal rat pups but not in bulbar 5-HT-depleted rat pups. A dose of 6 mg/kg isoproterenol, which was ineffective in modulating these measures in normal rat pups, induced odor preference learning, enhanced phosphorylated CREB (pCREB) expression, and increased ON-EFPs in bulbar 5-HT-depleted pups. These outcomes suggest that NE and 5-HT promote specific biochemical and electrophysiological changes that may critically underlie odor preference learning.

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  • During the first ten days of life, learning-associated plasticity for new maternal odors in rat pups occurs within the olfactory bulb and requires an odor paired with copious amounts of NE.

    “During the first ten days of life, the learning process for new maternal odors in rat pups occurs through a relatively simple neurobiological substrate. At this early age, learning-associated plasticity occurs within the olfactory bulb, the first relay station for olfactory processing. This process requires that an odor is paired with copious amounts of NE (Sullivan et al., 2000b, Sullivan et al.,”

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  1. Hebb, D.O. 1949. Title of Chapter 4. The Organization of Behavior: A Neurophysiological Theory. John Wiley, New York.

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Authors
Yuan, Q., Harley, C. W., Bruce, J. C., Darby-King, A., & McLean, J. H.
Affiliations
Memorial University of Newfoundland (CA)
Venue
Learning & Memory
Published
2000-11-01
Volume
7
Issue
6
Pages
413-421
ISSN
  • 1072-0502 (ISSN-L)
  • 1549-5485
OpenAlex
W1977774316
Cited by
73
References
46
Citations by year
2025: 1 · 2023: 1 · 2021: 1 · 2020: 1 · 2019: 1 · 2018: 1 · 2017: 2 · 2016: 5 · 2015: 2 · 2014: 3 · 2013: 1 · 2012: 7
Type
posted content
Language
en
Open access
Locations
  • Learning & Memory (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
Medical Research Council
Topics
  • Life Sciences › Neuroscience › Sensory Systems › Olfactory and Sensory Function Studies
  • Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurobiology and Insect Physiology Research
Keywords
isoproterenol, CREB phosphorylation, olfactory bulb, serotonin depletion, norepinephrine, beta-adrenergic agonists, noradrenergic modulation, long-term memory, neonatal rats
MeSH terms
Animals, Conditioning, Psychological, Evoked Potentials, Female, Isoproterenol, Learning, Male, Odorants, Olfactory Bulb, Olfactory Nerve, Phosphorylation, Serotonin, Rats, Sprague-Dawley, Cyclic AMP Response Element-Binding Protein, Rats

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