Isoproterenol Increases CREB Phosphorylation and Olfactory Nerve–Evoked Potentials in Normal and 5-HT-Depleted Olfactory Bulbs in Rat Pups Only at Doses That Produce Odor Preference Learning
Open accessAbstract
Norepinephrine (NE) and serotonin (5-HT) are important modulators of early odor preference learning. NE can act as an unconditioned stimulus (UCS), whereas 5-HT facilitates noradrenergic actions. In this study, we examined the phosphorylation of an important transcription factor, cAMP response element binding protein (CREB), which has been implicated in long-term-memory formation (McLean et al. 1999) during NE-induced odor preference learning in normal and olfactory bulb 5-HT-depleted rat pups. We also examined NE modulation of olfactory nerve-evoked field potentials (ON-EFPs) in anesthetized normal and bulbar 5-HT depleted pups. Systemic injection of 2 mg/kg isoproterenol (beta-adrenoceptor agonist) induced odor preference learning, enhanced pCREB expression in the olfactory bulbs at 10 min after odor pairing, and increased ON-EFPs in normal rat pups but not in bulbar 5-HT-depleted rat pups. A dose of 6 mg/kg isoproterenol, which was ineffective in modulating these measures in normal rat pups, induced odor preference learning, enhanced phosphorylated CREB (pCREB) expression, and increased ON-EFPs in bulbar 5-HT-depleted pups. These outcomes suggest that NE and 5-HT promote specific biochemical and electrophysiological changes that may critically underlie odor preference learning.
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During the first ten days of life, learning-associated plasticity for new maternal odors in rat pups occurs within the olfactory bulb and requires an odor paired with copious amounts of NE.
“During the first ten days of life, the learning process for new maternal odors in rat pups occurs through a relatively simple neurobiological substrate. At this early age, learning-associated plasticity occurs within the olfactory bulb, the first relay station for olfactory processing. This process requires that an odor is paired with copious amounts of NE (Sullivan et al., 2000b, Sullivan et al.,”
From Early life adversity during the infant sensitive period for attachment: Programming of behavioral neurobiology of threat processing and social behavior (2017) · cites this paper as Yuan et al., 2000
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- Authors
- Yuan, Q., Harley, C. W., Bruce, J. C., Darby-King, A., & McLean, J. H.
- Affiliations
- Memorial University of Newfoundland (CA)
- Venue
- Learning & Memory
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2000-11-01
- Volume
- 7
- Issue
- 6
- Pages
- 413-421
- ISSN
- 1072-0502 (ISSN-L)
- 1549-5485
- PMC
- PMC311343
- OpenAlex
- W1977774316
- Cited by
- 73
- References
- 46
- Citations by year
- 2025: 1 · 2023: 1 · 2021: 1 · 2020: 1 · 2019: 1 · 2018: 1 · 2017: 2 · 2016: 5 · 2015: 2 · 2014: 3 · 2013: 1 · 2012: 7
- Type
- posted content
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Learning & Memory (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Medical Research Council
- Topics
- Life Sciences › Neuroscience › Sensory Systems › Olfactory and Sensory Function Studies
- Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurobiology and Insect Physiology Research
- Keywords
- isoproterenol, CREB phosphorylation, olfactory bulb, serotonin depletion, norepinephrine, beta-adrenergic agonists, noradrenergic modulation, long-term memory, neonatal rats
- MeSH terms
- Animals, Conditioning, Psychological, Evoked Potentials, Female, Isoproterenol, Learning, Male, Odorants, Olfactory Bulb, Olfactory Nerve, Phosphorylation, Serotonin, Rats, Sprague-Dawley, Cyclic AMP Response Element-Binding Protein, Rats
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