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Characterization, Expression, and Immunohistochemical Localization of 5α-Reductase in Human Skin

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Luu-The, Van, Sugimoto, Yasuro, Puy, Libertad, Labrie, Yvan, Solache, Irma Lopez, Singh, Mohan, Labrie, Fernand·1994·Journal of Investigative Dermatology

DOI 10.1111/1523-1747.ep12371766

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Abstract

Human skin has been shown to contain a high level of 5 alpha-reductase activity, the enzyme that catalyses the conversion of the weak androgen testosterone into dihydrotestosterone, the most potent androgen. Because two types of 5 alpha-reductase genes have been characterized in humans, we have cloned 5 alpha-reductase cDNAs from adult human keratinocyte and skin fibroblast cDNA libraries to identify and gain better knowledge of the 5 alpha-reductase expressed in normal human skin. Nucleotide sequence analysis shows that the clones obtained correspond to the type I 5 alpha-reductase. RNase protection analysis using (poly A)+ RNA obtained from human skin and prostate also confirms that type I 5 alpha-reductase is the predominant type expressed in normal skin, whereas type II 5 alpha-reductase is the major form found in the prostate. Following polymerase chain reaction amplification of human keratinocyte and skin fibroblast cDNA, a low level of type II 5 alpha-reductase cDNA has been detected. Using antipeptide antibodies raised in rabbits against the peptide sequence covering amino acids 227 -240 to perform immunohistochemical localization of 5 alpha-reductase, we have found that 5 alpha-reductase is distributed in sweat and sebaceous glands, as well as in the epidermal cell layers, thus providing the basis for the important role of androgens in human skin and its appendages.

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Authors: Luu-The, V., Sugimoto, Y., Puy, L., Labrie, Y., Solache, I. L., Singh, M., & Labrie, F.
Affiliations: Université Laval (CA)
ORCID: Sugimoto, Yasuro
Singh, Mohan
Venue: Journal of Investigative Dermatology
Publisher: Elsevier BV
Published: 1994-02
Volume: 102
Issue: 2
Pages: 221-226
ISSN: 0022-202X (ISSN-L)
DOI: 10.1111/1523-1747.ep12371766
OpenAlex: W2022602285
Cited by: 118
References: 54
Citations by year: 2026: 1 · 2023: 2 · 2018: 1 · 2017: 2 · 2016: 2 · 2015: 3 · 2014: 3 · 2013: 1
FWCI: 12.49
Type: journal article
Language: en
Open access: bronze
doi.org
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
www.elsevier.com/open-access/userlicense/1.0/ (vor, 7974 d)
Locations: Journal of Investigative Dermatology (publishedVersion)
PubMed (publishedVersion)
Topics: Health Sciences › Medicine › Dermatology › Hair Growth and Disorders
Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Prostate Cancer Treatment and Research
Health Sciences › Medicine › Reproductive Medicine › Sperm and Testicular Function
Keywords: 5α-reductase, human skin, keratinocytes, dermal fibroblasts, dihydrotestosterone, androgen metabolism, sweat glands, sebaceous glands, epidermis, immunolocalization, cDNA cloning, prostate
MeSH terms: Base Sequence, Cells, Cultured, DNA, Fibroblasts, Humans, Immunohistochemistry, Male, Molecular Sequence Data, Oxidoreductases, Prostate, Sebaceous Glands, Skin, Sweat Glands, Immunoblotting, Keratinocytes, Polymerase Chain Reaction, Cholestenone 5 alpha-Reductase

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Characterization, Expression, and Immunohistochemical Localization of 5α-Reductase in Human Skin

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