Effects of testosterone treatment on markers of skeletal muscle ribosome biogenesis
Open accessAbstract
The effects of testosterone (TEST) treatment on markers of skeletal muscle ribosome biogenesis in vitro and in vivo were examined. C2C12 myotubes were treated with 100 nm TEST for short-term (24-h) and longer-term (96-h) treatments. Moreover, male 10-month-old Fischer 344 rats were housed for 4 weeks, and the following groups were included in this study: (i) Sham-operated (Sham) rats, (ii) orchiectomised rats (ORX) and (iii) ORX+TEST-treated rats (7.0 mg week−1). For in vitro data, TEST treatment increased c-Myc mRNA expression by 38% (P = 0.004) after 96 h, but did not affect total RNA, 47S pre-rRNA, Raptor mRNA, Nop56 mRNA, Bop1 mRNA, Ncl mRNA at 24 h or 96 h following the treatment. For in vivo data, ORX decreased levator ani/bulbocavernosus (LABC) myofibril protein versus Sham (P = 0.006), whereas ORX+TEST (P = 0.015) rescued this atrophic effect. ORX also decreased muscle ribosome content (total RNA) compared to Sham (P = 0.046), whereas ORX+TEST tended to rescue this effect (P = 0.057). However, other markers of ribosome biogenesis including c-Myc mRNA, Nop56 mRNA, Bop1 mRNA, Ncl mRNA decreased with ORX independently of TEST treatments (P < 0.05). Finally, lower phospho-(Ser235/236)-to-total rps6 protein and lower rpl5 protein levels existed in ORX+TEST rats versus other treatments, suggesting that chronic TEST treatment may lower translational capacity.
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In rodents, testosterone helps preserve muscle mass during aging by regulating ribosomal content, which is needed for protein synthesis.
“Therefore, at least in rodents, T plays an important role in maintaining muscle mass during aging through regulating ribosomal content, which is critical for protein synthesis.”
From Hormonal and Metabolic Changes of Aging and the Influence of Lifestyle Modifications (2021) · cites this paper as [157]
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Roberts, M. D., McCarthy, J. J., Hornberger, T. A., Phillips, S. M., Mackey, A. L., Nader, G. A., Boppart, M. D., Kavazis, A. N., Reidy, P. T., Ogasawara, R., et al. (2023) · Physiological Reviews · doi:10.1152/physrev.00039.2022 · Cited by 214 · see citing works
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- Authors
- Mobley, C. B., Mumford, P. W., Kephart, W. C., Conover, C. F., Beggs, L. A., Balaez, A., Yarrow, J. F., Borst, S. E., Beck, D. T., & Roberts, M. D.
- Affiliations
- Auburn University (US)
- North Florida/South Georgia Veterans Health System (US)
- University of Florida (US)
- Edward Via College of Osteopathic Medicine (US)
- Corresponding author
- Roberts, M. D.
- Venue
- Andrologia
- Publisher
- Hindawi Limited
- Published
- 2016-01-19
- Volume
- 48
- Issue
- 9
- Pages
- 1055-1065
- ISSN
- 0303-4569 (ISSN-L)
- OpenAlex
- W2297682178
- Cited by
- 17
- References
- 37
- Citations by year
- 2025: 1 · 2023: 1 · 2022: 1 · 2021: 1 · 2020: 4 · 2019: 1 · 2018: 1 · 2017: 6 · 2016: 1
- FWCI
- 3.02
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Andrologia (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Veteran's Health Administration Rehabilitation R&D
- Topics
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Hormonal and reproductive studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Muscle metabolism and nutrition
- Keywords
- testosterone, skeletal muscle, ribosome biogenesis, orchiectomy, muscle atrophy, c-Myc, pre-rRNA, NOP56, BOP1, NCL, S6 phosphorylation, RPL5, translational capacity, C2C12 myotubes, F344 rats
- MeSH terms
- Androgens, Animals, Cell Line, Male, Muscle Proteins, Orchiectomy, Rats, Inbred F344, Ribosomal Proteins, Ribosomes, RNA, Messenger, RNA, Ribosomal, Testosterone, Biomarkers, Muscle, Skeletal, Muscle Development, Rats
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