Cited by 17Hormonal and reproductive studiesMuscle Physiology and DisordersMuscle metabolism and nutrition

Effects of testosterone treatment on markers of skeletal muscle ribosome biogenesis

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Mobley, C. B., Mumford, P. W., Kephart, W. C., Conover, C. F., Beggs, L. A., Balaez, A., Yarrow, J. F., Borst, S. E. +2 more·2016·Andrologia

Abstract

The effects of testosterone (TEST) treatment on markers of skeletal muscle ribosome biogenesis in vitro and in vivo were examined. C2C12 myotubes were treated with 100 nm TEST for short-term (24-h) and longer-term (96-h) treatments. Moreover, male 10-month-old Fischer 344 rats were housed for 4 weeks, and the following groups were included in this study: (i) Sham-operated (Sham) rats, (ii) orchiectomised rats (ORX) and (iii) ORX+TEST-treated rats (7.0 mg week−1). For in vitro data, TEST treatment increased c-Myc mRNA expression by 38% (P = 0.004) after 96 h, but did not affect total RNA, 47S pre-rRNA, Raptor mRNA, Nop56 mRNA, Bop1 mRNA, Ncl mRNA at 24 h or 96 h following the treatment. For in vivo data, ORX decreased levator ani/bulbocavernosus (LABC) myofibril protein versus Sham (P = 0.006), whereas ORX+TEST (P = 0.015) rescued this atrophic effect. ORX also decreased muscle ribosome content (total RNA) compared to Sham (P = 0.046), whereas ORX+TEST tended to rescue this effect (P = 0.057). However, other markers of ribosome biogenesis including c-Myc mRNA, Nop56 mRNA, Bop1 mRNA, Ncl mRNA decreased with ORX independently of TEST treatments (P < 0.05). Finally, lower phospho-(Ser235/236)-to-total rps6 protein and lower rpl5 protein levels existed in ORX+TEST rats versus other treatments, suggesting that chronic TEST treatment may lower translational capacity.

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  • In rodents, testosterone helps preserve muscle mass during aging by regulating ribosomal content, which is needed for protein synthesis.

    “Therefore, at least in rodents, T plays an important role in maintaining muscle mass during aging through regulating ribosomal content, which is critical for protein synthesis.”

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Authors
Mobley, C. B., Mumford, P. W., Kephart, W. C., Conover, C. F., Beggs, L. A., Balaez, A., Yarrow, J. F., Borst, S. E., Beck, D. T., & Roberts, M. D.
Affiliations
  • Auburn University (US)
  • North Florida/South Georgia Veterans Health System (US)
  • University of Florida (US)
  • Edward Via College of Osteopathic Medicine (US)
Corresponding author
Roberts, M. D.
Venue
Andrologia
Published
2016-01-19
Volume
48
Issue
9
Pages
1055-1065
ISSN
0303-4569 (ISSN-L)
OpenAlex
W2297682178
Cited by
17
References
37
Citations by year
2025: 1 · 2023: 1 · 2022: 1 · 2021: 1 · 2020: 4 · 2019: 1 · 2018: 1 · 2017: 6 · 2016: 1
FWCI
3.02
Type
journal article
Language
en
Open access
Locations
  • Andrologia (publishedVersion)
  • PubMed (publishedVersion)
Funders
Veteran's Health Administration Rehabilitation R&D
Topics
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Hormonal and reproductive studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Muscle metabolism and nutrition
Keywords
testosterone, skeletal muscle, ribosome biogenesis, orchiectomy, muscle atrophy, c-Myc, pre-rRNA, NOP56, BOP1, NCL, S6 phosphorylation, RPL5, translational capacity, C2C12 myotubes, F344 rats
MeSH terms
Androgens, Animals, Cell Line, Male, Muscle Proteins, Orchiectomy, Rats, Inbred F344, Ribosomal Proteins, Ribosomes, RNA, Messenger, RNA, Ribosomal, Testosterone, Biomarkers, Muscle, Skeletal, Muscle Development, Rats

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