Characterization of the metabolic effects of irisin on skeletal muscle in vitro
Abstract
AIMS: This work explored the effects of irisin on metabolism, gene expression and mitochondrial content in cultured myocytes. METHODS: C2C12 myocytes were treated with various concentrations of irisin for various durations. Glycolysis and oxidative metabolism were quantified by measurement of extracellular acidification and oxygen consumption, respectively. Metabolic gene expression was measured by quantitative real-time polymerase chain reaction (qRT-PCR) and mitochondrial content was assessed by flow cytometry and confocal microscopy. RESULTS: Cells treated with irisin exhibited significantly increased oxidative metabolism. Irisin treatment also significantly increased mitochondrial uncoupling at various doses and durations. Lastly, treatment with irisin also significantly elevated metabolic gene expression including peroxisome proliferator-activated receptor γ coactivator-1 alpha (PGC-1α), nuclear respiratory factor 1 (NRF1), mitochondrial transcription factor A (TFAM), irisin, glucose transporter 4 (GLUT4) and mitochondrial uncoupling protein 3 (UCP3) leading to increased mitochondrial biogenesis. CONCLUSIONS: Our observations are the first to document increased metabolism in myocytes through irisin-mediated induction of mitochondrial biogenesis and uncoupling with corresponding gene expression. These observations support the need for further investigation into the therapeutic and pharmacological effects of irisin, as well as development of irisin-based therapy.
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Irisin stimulates lipolysis via HSL and inhibits lipid accumulation in adipocytes.
“Irisin was described to stimulate lipolysis via HSL and to inhibit lipid accumulation in adipocytes [58,64].”
Cites
A new mathematical model for relative quantification in real-time RT-PCR
Pfaffl, M. W. (2001) · Nucleic Acids Research · doi:10.1093/nar/29.9.e45 · Cited by 34,610 · see citing works
A PGC1-α-dependent myokine that drives brown-fat-like development of white fat and thermogenesis
Boström, P., Wu, J., Jedrychowski, M. P., Korde, A., Ye, L., Lo, J. C., Rasbach, K. A., Boström, E. A., Choi, J. H., Long, J. Z., et al. (2012) · Nature · doi:10.1038/nature10777 · Cited by 4,810 · see citing works
Wu, Z., Puigserver, P., Andersson, U., Zhang, C., Adelmant, G., Mootha, V., Troy, A., Cinti, S., Lowell, B., Scarpulla, R. C., et al. (1999) · Cell · doi:10.1016/s0092-8674(00)80611-x · Cited by 4,139 · see citing works
Beige Adipocytes Are a Distinct Type of Thermogenic Fat Cell in Mouse and Human
Wu, J., Boström, P., Sparks, L. M., Li, Y., Choi, J. H., Giang, A.-H., Khandekar, M. J., Virtanen, K. A., Nuutila, P., Schaart, G., et al. (2012) · Cell · doi:10.1016/j.cell.2012.05.016 · Cited by 3,299 · see citing works
Muscles, exercise and obesity: skeletal muscle as a secretory organ
Pedersen, B. K., & Febbraio, M. A. (2012) · Nature Reviews Endocrinology · doi:10.1038/nrendo.2012.49 · Cited by 2,909 · see citing works
Virbasius, J. V., & Scarpulla, R. C. (1994) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.91.4.1309 · Cited by 819 · see citing works
Moreno-Navarrete, J. M., Ortega, F., Serrano, M., Guerra, E., Pardo, G., Tinahones, F., Ricart, W., & Fernández-Real, J. M. (2013) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2012-2749 · Cited by 788 · see citing works
Gleyzer, N., Vercauteren, K., & Scarpulla, R. C. (2005) · Molecular and Cellular Biology · doi:10.1128/mcb.25.4.1354-1366.2005 · Cited by 711 · see citing works
Regulation of mitochondrial biogenesis in skeletal muscle by CaMK
Wu, H., Kanatous, S. B., Thurmond, F. A., Gallardo, T., Isotani, E., Bassel-Duby, R., & Williams, R. S. (2002) · Science · doi:10.1126/science.1071163 · Cited by 684 · see citing works
NRF-1: a trans-activator of nuclear-encoded respiratory genes in animal cells.
Evans, M. J., & Scarpulla, R. C. (1990) · Genes & Development · doi:10.1101/gad.4.6.1023 · Cited by 417 · see citing works
Scarpulla, R. (2002) · Gene · doi:10.1016/s0378-1119(01)00809-5 · Cited by 371 · see citing works
A Tissue-Specific Coactivator of Steroid Receptors, Identified in a Functional Genetic Screen
Knutti, D., Kaul, A., & Kralli, A. (2000) · Molecular and Cellular Biology · doi:10.1128/mcb.20.7.2411-2422.2000 · Cited by 286 · see citing works
Castillo-Quan, J. I. (2012) · Disease Models & Mechanisms · doi:10.1242/dmm.009894 · Cited by 157 · see citing works
Kelly, D. P. (2012) · Science · doi:10.1126/science.1221688 · Cited by 132 · see citing works
Irisin: A renaissance in metabolism?
Polyzos, S. A., Kountouras, J., Shields, K., & Mantzoros, C. S. (2013) · Metabolism · doi:10.1016/j.metabol.2013.04.008 · Cited by 131 · see citing works
A Novel High-Throughput Assay for Islet Respiration Reveals Uncoupling of Rodent and Human Islets
Wikström, J. D., Sereda, S. B., Stiles, L., Elorza, Á. A., Allister, E. M., Neilson, A., Ferrick, D. A., Wheeler, M. B., & Shirihai, O. S. (2012) · PLoS ONE · doi:10.1371/journal.pone.0033023 · Cited by 123 · see citing works
Villarroya, F. (2012) · Cell Metabolism · doi:10.1016/j.cmet.2012.02.010 · Cited by 104 · see citing works
Vaughan, R. A., Garcia‐Smith, R., Gannon, N. P., Bisoffi, M., Trujillo, K. A., & Conn, C. A. (2013) · Amino Acids · doi:10.1007/s00726-013-1538-5 · Cited by 60 · see citing works
Self-citationMetabolic pathways inAnopheles stephensimitochondria
Giulivi, C., Ross‐Inta, C., Horton, A. A., & Luckhart, S. (2008) · Biochemical Journal · doi:10.1042/bj20080973 · Cited by 43 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Skeletal muscle inflammation and insulin resistance in obesity
Wu, H., & Ballantyne, C. M. (2017) · Journal of Clinical Investigation · doi:10.1172/jci88880 · Cited by 695 · see citing works
Ruegsegger, G. N., & Booth, F. W. (2018) · Cold Spring Harbor Perspectives in Medicine · doi:10.1101/cshperspect.a029694 · Cited by 690 · see citing works
Physiology and role of irisin in glucose homeostasis
Perakakis, N., Triantafyllou, G. A., Fernández-Real, J. M., Huh, J. Y., Park, K. H., Seufert, J., & Mantzoros, C. S. (2017) · Nature Reviews Endocrinology · doi:10.1038/nrendo.2016.221 · Cited by 621 · see citing works
Skeletal muscle as an endocrine organ: PGC-1α, myokines and exercise
Schnyder, S., & Handschin, C. (2015) · Bone · doi:10.1016/j.bone.2015.02.008 · Cited by 419 · see citing works
Huh, J. Y., Mougios, V., Kabasakalis, A., Fatouros, I., Siopi, A., Douroudos, I. I., Filippaios, A., Panagiotou, G., Park, K. H., & Mantzoros, C. S. (2014) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2014-1437 · Cited by 363 · see citing works
Qiao, X., Nie, Y., Ma, Y., Chen, Y., Cheng, R., Yin, W., Hu, Y., Xu, W., & Xu, L. (2016) · Scientific Reports · doi:10.1038/srep18732 · Cited by 291 · see citing works
Myokines and Adipokines: Involvement in the Crosstalk between Skeletal Muscle and Adipose Tissue
Li, F., Li, Y., Duan, Y., Hu, C.-A. A., Tang, Y., & Yin, Y. (2017) · Cytokine & Growth Factor Reviews · doi:10.1016/j.cytogfr.2016.10.003 · Cited by 288 · see citing works
Gonzalez-Gil, A. M., & Elizondo-Montemayor, L. (2020) · Nutrients · doi:10.3390/nu12061899 · Cited by 278 · see citing works
“Irisin Ob: ↑ [39]+; T2DM: ↓ [39]+ AE: ↑ (aerobic or resistance) [40]+ CE: ↓? (aerobic or resistance) [41]+ ↑ (via PGC-1α): cold-induced shivering [42], ↑AMP:ATP ratio (via AMPK-PGC-1α pathway), FGF21* [43], and METRNL (via ↑PGC-1α4)* [44] ↓: proinflammatory cytokines [45] (possibly via NF-kB-mediated inhibition of PGC…”
BackgroundMyokines: The endocrine coupling of skeletal muscle and bone
Gomarasca, M., Banfi, G., & Lombardi, G. (2019) · Advances in clinical chemistry · doi:10.1016/bs.acc.2019.07.010 · Cited by 274 · see citing works
Irisin in response to exercise in humans with and without metabolic syndrome
Huh, J. Y., Siopi, A., Mougios, V., Park, K. H., & Mantzoros, C. S. (2015) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2014-2416 · Cited by 210 · see citing works
Crosstalk between adipokines and myokines in fat browning
Rodrı́guez, A., Becerril, S., Ezquerro, S., Méndez-Giménez, L., & Frühbeck, G. (2016) · Acta Physiologica · doi:10.1111/apha.12686 · Cited by 192 · see citing works
Gannon, N. P., Vaughan, R. A., Garcia‐Smith, R., Bisoffi, M., & Trujillo, K. A. (2014) · International Journal of Cancer · doi:10.1002/ijc.29142 · Cited by 187 · see citing works
Self-citationRoles of myokines in exercise-induced improvement of neuropsychiatric function
Kim, S., Choi, J., Moon, S., Park, D., Kwak, H., & Kang, J. (2019) · Pflügers Archiv - European Journal of Physiology · doi:10.1007/s00424-019-02253-8 · Cited by 168 · see citing works
Peng, K.-G., Yang, L.-K., Wang, J., Ye, F., Dan, G., Zhao, Y.-P., Cai, Y., Cui, Z., Ao, L., Liu, J.-Y., et al. (2017) · Molecular Neurobiology · doi:10.1007/s12035-016-9944-9 · Cited by 155 · see citing works
Irisin Inhibits Hepatic Cholesterol Synthesis via AMPK-SREBP2 Signaling
Tang, H. W., Yu, R., Liu, S., Huwatibieke, B., Li, Z., & Zhang, W. (2016) · EBioMedicine · doi:10.1016/j.ebiom.2016.02.041 · Cited by 151 · see citing works
Multiple Roles in Neuroprotection for the Exercise Derived Myokine Irisin
Farshbaf, M. J., & Alviña, K. (2021) · Frontiers in Aging Neuroscience · doi:10.3389/fnagi.2021.649929 · Cited by 143 · see citing works
Mechanisms by Which Skeletal Muscle Myokines Ameliorate Insulin Resistance
Balakrishnan, R., & Thurmond, D. C. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms23094636 · Cited by 132 · see citing works
Rodrı́guez, A., Becerril, S., Méndez-Giménez, L., Ramírez, B., Sáinź, N., Catalán, V., Gómez‐Ambrosi, J., & Frühbeck, G. (2014) · International Journal of Obesity · doi:10.1038/ijo.2014.166 · Cited by 130 · see citing works
Irisin a Novel Metabolic Biomarker: Present Knowledge and Future Directions
Muñoz, I. Y. M., Camarillo-Romero, E. D. S., & Garduño‐García, J. D. J. (2018) · International Journal of Endocrinology · doi:10.1155/2018/7816806 · Cited by 116 · see citing works
Gamas, L., Matafome, P., & Seiça, R. (2015) · Journal of Diabetes Research · doi:10.1155/2015/359159 · Cited by 114 · see citing works
An update on the role of irisin in the regulation of endocrine and metabolic functions
Mahgoub, M., D’Souza, C. M., Darmaki, R. S. A., Baniyas, M. M. Y. H., & Adeghate, E. A. (2018) · Peptides · doi:10.1016/j.peptides.2018.03.018 · Cited by 109 · see citing works
Role of Irisin in Myocardial Infarction, Heart Failure, and Cardiac Hypertrophy
Ho, M.-Y., & Wang, C. (2021) · Cells · doi:10.3390/cells10082103 · Cited by 109 · see citing works
Biological Aspects of Selected Myokines in Skeletal Muscle: Focus on Aging
Mancinelli, R., Checcaglini, F., Coscia, F., Gigliotti, P. V., Fulle, S., & Fanò, G. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22168520 · Cited by 108 · see citing works
Guo, B., Zhang, Z.-K., Liang, C., Li, J., Liu, J., Lu, A., Zhang, B.-T., & Zhang, G. (2017) · Calcified Tissue International · doi:10.1007/s00223-016-0209-4 · Cited by 96 · see citing works
The Role of Exercise, Diet, and Cytokines in Preventing Obesity and Improving Adipose Tissue
ATAKAN, M. M., Koşar, Ş. N., Güzel, Y., Tin, H. T., & Yan, X. (2021) · Nutrients · doi:10.3390/nu13051459 · Cited by 94 · see citing works
Garneau, L., & Aguer, C. (2019) · Diabetes & Metabolism · doi:10.1016/j.diabet.2019.02.006 · Cited by 92 · see citing works
Is irisin the new player in exercise-induced adaptations or not? A 2017 update
Fatouros, I. G. (2017) · Clinical Chemistry and Laboratory Medicine (CCLM) · doi:10.1515/cclm-2017-0674 · Cited by 91 · see citing works
Harnessing Muscle–Liver Crosstalk to Treat Nonalcoholic Steatohepatitis
Chakravarthy, M. V., Siddiqui, M. S., Forsgren, M. F., & Sanyal, A. J. (2020) · Frontiers in Endocrinology · doi:10.3389/fendo.2020.592373 · Cited by 89 · see citing works
Ellefsen, S., Vikmoen, O., Slettaløkken, G., Whist, J. E., Nygaard, H., Hollan, I., Rauk, I., Vegge, G., Strand, T. A., Raastad, T., et al. (2014) · European Journal of Applied Physiology · doi:10.1007/s00421-014-2922-x · Cited by 89 · see citing works
Searching for the link between inflammaging and sarcopenia
Wang, T. (2022) · Ageing Research Reviews · doi:10.1016/j.arr.2022.101611 · Cited by 88 · see citing works
Decreased irisin secretion contributes to muscle insulin resistance in high-fat diet mice.
Yang, Z., Xu, C., Chen, Y., & Zhao, Q. (2015) · Europe PMC (PubMed Central) · Cited by 86 · see citing works
Irisin, a unique non-inflammatory myokine in stimulating skeletal muscle metabolism
Vaughan, R. A., Gannon, N. P., Mermier, C. M., & Conn, C. A. (2015) · Journal of Physiology and Biochemistry · doi:10.1007/s13105-015-0433-9 · Cited by 77 · see citing works
Self-citationIrisin reverses insulin resistance in C2C12 cells via the p38-MAPK-PGC-1α pathway
Ye, X., Shen, Y., Ni, C., Ye, J., Xin, Y., Zhang, W., & Ren, Y. (2019) · Peptides · doi:10.1016/j.peptides.2019.170120 · Cited by 72 · see citing works
Chemello, F., Grespi, F., Zulian, A., Cancellara, P., Hébert-Chatelain, É., Martini, P. G. V., Bean, C., Alessio, E., Buson, L., Bazzega, M., et al. (2019) · Cell Reports · doi:10.1016/j.celrep.2019.02.105 · Cited by 72 · see citing works
Irisin Exerts Inhibitory Effect on Adipogenesis Through Regulation of Wnt Signaling
Ma, E. B., Sahar, N.-E., Jeong, M., & Huh, J. Y. (2019) · Frontiers in Physiology · doi:10.3389/fphys.2019.01085 · Cited by 69 · see citing works
Li, D., Huang, F., Lu, W., Jiang, G. ‐., Deng, Y., & Shen, F. (2014) · Acta Physiologica · doi:10.1111/apha.12421 · Cited by 65 · see citing works
Peterson, J. M., Mart, R., & Bond, C. E. (2014) · PeerJ · doi:10.7717/peerj.605 · Cited by 64 · see citing works
Gao, S., Li, F., Li, H., Yi, H., Liu, Y., & Chen, Y. (2016) · PLoS ONE · doi:10.1371/journal.pone.0147480 · Cited by 63 · see citing works
Anti-diabetic activity of recombinant irisin in STZ-induced insulin-deficient diabetic mice
Duan, H., Ma, B., Ma, X., Wang, H., Ni, Z., Wang, B., Li, X., Jiang, P., Umar, M., & Li, M. (2015) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2015.12.049 · Cited by 62 · see citing works
Pérez-Sotelo, D., Roca-Rivada, A., de la Torre, I. B., Baltar, J., Castro, A. I., Domínguez, E., Collado, M., Casanueva, F. F., & Pardo, M. (2017) · Scientific Reports · doi:10.1038/s41598-017-16602-z · Cited by 59 · see citing works
Lower circulating irisin is associated with nonalcoholic fatty liver disease and type 2 diabetes
Shanaki, M., Moradi, N., Emamgholipour, S., Fadaei, R., & Poustchi, H. (2017) · Diabetes & Metabolic Syndrome Clinical Research & Reviews · doi:10.1016/j.dsx.2017.03.037 · Cited by 57 · see citing works
García-Fontana, B., Reyes‐García, R., Morales, S., Ávila-Rubio, V., Muñoz‐Garach, A., Rozas-Moreno, P., & Muñoz‐Torres, M. (2015) · Endocrine · doi:10.1007/s12020-015-0758-8 · Cited by 54 · see citing works
Nigro, E., Scudiero, O., Monaco, M. L., Polito, R., Schettino, P., Grandone, A., Perrone, L., del Giudice, E. M., & Daniele, A. (2017) · Cytokine · doi:10.1016/j.cyto.2016.12.018 · Cited by 54 · see citing works
Sarcopenia and cachexia: molecular mechanisms and therapeutic interventions
Wang, T., Zhou, D., & Hong, Z. (2025) · MedComm · doi:10.1002/mco2.70030 · Cited by 46 · see citing works
The Physiological Role of Irisin in the Regulation of Muscle Glucose Homeostasis
Yano, N., Zhao, Y., & Zhao, T. C. (2021) · Endocrines · doi:10.3390/endocrines2030025 · Cited by 46 · see citing works
Butt, Z. D., Hackett, J. D., & Volkoff, H. (2017) · Peptides · doi:10.1016/j.peptides.2017.02.003 · Cited by 44 · see citing works
Kolodziejski, P. A., Pruszyńska‐Oszmałek, E., Wojciechowicz, T., Sassek, M., Leciejewska, N., Jasaszwili, M., Billert, M., Małek, E., Szczepankiewicz, D., Misiewicz-Mielnik, M., et al. (2021) · Genes · doi:10.3390/genes12050756 · Cited by 43 · see citing works
Pervin, S., Reddy, S. T., & Singh, R. (2021) · Frontiers in Endocrinology · doi:10.3389/fendo.2021.653179 · Cited by 42 · see citing works
Shero, J. A., Lindholm, M. E., Sandri, M. F., & Stanford, K. I. (2025) · Circulation Research · doi:10.1161/circresaha.124.325614 · Cited by 43 · see citing works
Delgado, A. S. H., Roffe-Vazquez, D. N., González-Gil, A. M., Villarreal-Calderón, J. R., Tamez‐Rivera, O., Rodríguez-Gutiérrez, N. A., Castillo, E. C., Silva‐Platas, C., García‐Rivas, G., & Elizondo‐Montemayor, L. (2020) · Journal of Diabetes Research · doi:10.1155/2020/1949415 · Cited by 40 · see citing works
Dihydromyricetin stimulates irisin secretion partially via the PGC-1α pathway
Zhou, Q., Chen, K., Liu, P., Gao, Y., Zou, D., Deng, H., Huang, Y., Zhang, Q., Zhu, J., & Mi, M. (2015) · Molecular and Cellular Endocrinology · doi:10.1016/j.mce.2015.05.036 · Cited by 39 · see citing works
Effect of Long-Term Moderate Physical Exercise on Irisin between Normal Weight and Obese Men
Rashid, F. A., Abbas, H. J., Naser, N. A., & Ali, H. A. (2020) · The Scientific World JOURNAL · doi:10.1155/2020/1897027 · Cited by 39 · see citing works
The Metabolic Effects of Traditional Chinese Medication Qiliqiangxin on H9C2 Cardiomyocytes
Lin, S., Wu, X., Tao, L., Bei, Y., Zhang, H., Zhou, Y., Shen, S., Xiao, J., & Li, X. (2015) · Cellular Physiology and Biochemistry · doi:10.1159/000438580 · Cited by 38 · see citing works
Irisin Has a Protective Role against Osteoporosis in Ovariectomized Rats
Morgan, E. N., Alsharidah, A. S., Mousa, A., & Edrees, H. M. (2021) · BioMed Research International · doi:10.1155/2021/5570229 · Cited by 37 · see citing works
Safarimosavi, S., Mohebbi, H., & Rohani, H. (2018) · The Journal of Strength and Conditioning Research · doi:10.1519/jsc.0000000000002709 · Cited by 35 · see citing works
Tack, W., Cock, A. D., Dirinck, E. L., Bastijns, S., Ariën, F., & Perkisas, S. (2023) · Diabetes Obesity and Metabolism · doi:10.1111/dom.15321 · Cited by 34 · see citing works
Role of Irisin in Endocrine and Metabolic Disorders—Possible New Therapeutic Agent?
Leuștean, L., Preda, C., Teodoriu, L. C., Mihalache, L., Arhire, L. I., & Ungureanu, M.-C. (2021) · Applied Sciences · doi:10.3390/app11125579 · Cited by 33 · see citing works
Recent Advances in Nutraceuticals for the Treatment of Sarcopenic Obesity
Kim, Y.-C., Ki, S.-W., Kim, H., Kang, S., Kim, H., & Go, G. (2023) · Nutrients · doi:10.3390/nu15173854 · Cited by 33 · see citing works
The interplay between muscle mass decline, obesity, and type 2 diabetes.
Maliszewska, K., Adamska-Patruno, E., & Krętowski, A. (2019) · Polish Archives of Internal Medicine · doi:10.20452/pamw.15025 · Cited by 35 · see citing works
Circumventing the Crabtree effect in cell culture: A systematic review
de Kok, M. J. C., Schaapherder, A. F., Wüst, R. C. I., Zuiderwijk, M. C., Bakker, J. A., Lindeman, J. H. N., & Dévédec, S. E. L. (2021) · Mitochondrion · doi:10.1016/j.mito.2021.03.014 · Cited by 32 · see citing works
Riley, L. A., & Esser, K. A. (2017) · Current Osteoporosis Reports · doi:10.1007/s11914-017-0363-2 · Cited by 32 · see citing works
Li, X., Fang, W., Hu, Y., Wang, Y., & Li, J. (2015) · Gene · doi:10.1016/j.gene.2015.06.022 · Cited by 31 · see citing works
Codon optimization and expression of irisin in Pichia pastoris GS115
Duan, H., Wang, H., Ma, B., Jiang, P., Tu, P., Ni, Z., Li, X., Li, M., 麻晓峰, Wang, B., et al. (2015) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2015.04.030 · Cited by 31 · see citing works
Irisin Protects Musculoskeletal Homeostasis via a Mitochondrial Quality Control Mechanism
Zhao, C., Wu, Y., Zhu, S., Liu, H., & Xu, S. (2024) · International Journal of Molecular Sciences · doi:10.3390/ijms251810116 · Cited by 30 · see citing works
Irisin and Energy Metabolism and the Role of Irisin on Metabolic Syndrome
Aladag, T., Moğulkoç, R., & Baltacı, A. K. (2023) · Mini-Reviews in Medicinal Chemistry · doi:10.2174/1389557523666230411105506 · Cited by 30 · see citing works
Srinivasa, S., Suresh, C., Mottla, J. L., Hamarneh, S. R., Irazoqui, J. E., Frontera, W. R., Torriani, M., Stanley, T. L., & Makimura, H. (2015) · Growth Hormone & IGF Research · doi:10.1016/j.ghir.2015.12.008 · Cited by 29 · see citing works
Travis, C., Srivastava, P. S., Hawke, T. J., & Kalaitzoglou, E. (2022) · Journal of Diabetes Research · doi:10.1155/2022/2650342 · Cited by 27 · see citing works
Motta, V. F., Bargut, T. C. L., Águila, M. B., & Mandarim‐de‐Lacerda, C. A. (2017) · Journal of Applied Physiology · doi:10.1152/japplphysiol.00154.2017 · Cited by 27 · see citing works
Abdi, A., Mehrabani, J., Nordvall, M., Wong, A., Fallah, A., & Bagheri, R. (2020) · Archives of Physiology and Biochemistry · doi:10.1080/13813455.2020.1716018 · Cited by 26 · see citing works
Exercise-Induced Circulating Irisin Level Is Correlated with Improved Cardiac Function in Rats
Seo, D. Y., Bae, J. H., Kim, T. N., Kwak, H., Kha, P. T., & Han, J. (2020) · International Journal of Environmental Research and Public Health · doi:10.3390/ijerph17113863 · Cited by 26 · see citing works
Hénin, G., Lanthier, N., & Dahlqvist, G. (2022) · Acta gastro-enterologica belgica · doi:10.51821/85.4.10899 · Cited by 25 · see citing works
Fighting obesity: When muscle meets fat
Yang, X., Bi, P., & Kuang, S. (2014) · Adipocyte · doi:10.4161/21623945.2014.964075 · Cited by 25 · see citing works
Żebrowska, A., Sikora, M., Konarska-Rawluk, A., Zwierzchowska, A., Kamiński, T., Robins, A., & Hall, B. (2020) · Therapeutic Advances in Endocrinology and Metabolism · doi:10.1177/2042018820925326 · Cited by 24 · see citing works
Exercise and Metabolic Health: The Emerging Roles of Novel Exerkines
Yazgan, B., Türkel, İ., Özerkliğ, B., ATAKAN, M. M., Aktitiz, S., & Koşar, Ş. N. (2022) · Current Protein and Peptide Science · doi:10.2174/1389203723666220629163524 · Cited by 25 · see citing works
The Role of Irisin throughout Women’s Life Span
Barbagallo, F., Cannarella, R., Garofalo, V., Marino, M., Vignera, S. L., Condorelli, R. A., Tiranini, L., Nappi, R. E., & Calogero, A. E. (2023) · Biomedicines · doi:10.3390/biomedicines11123260 · Cited by 24 · see citing works
Qiu, R., Sun, W., Su, Y., Sun, Z., Fan, K., Liang, Y., Lin, X., & Zhang, Y. (2024) · Life Sciences · doi:10.1016/j.lfs.2024.123088 · Cited by 23 · see citing works
Muscle-UCP3 in the regulation of energy metabolism
Guardia, L. D., Luzi, L., & Codella, R. (2024) · Mitochondrion · doi:10.1016/j.mito.2024.101872 · Cited by 22 · see citing works
Zhang, Y., Luo, C., Huang, P., Cheng, Y., Ma, Y., Gao, J., & Ding, H. (2024) · Journal of Nutritional Biochemistry · doi:10.1016/j.jnutbio.2024.109780 · Cited by 20 · see citing works
Lagzdiņa, R., Rumaka, M., Gersone, G., & Tretjakovs, P. (2020) · Medicina · doi:10.3390/medicina56060274 · Cited by 22 · see citing works
Irisin vs. Treadmill Exercise in Post Myocardial Infarction Cardiac Rehabilitation in Rats
Hassaan, P. S., Nassar, S. Z., Issa, Y. A., & Zahran, N. M. (2019) · Archives of Medical Research · doi:10.1016/j.arcmed.2019.05.009 · Cited by 21 · see citing works
Nazem, S., Rabiee, F., Ghaedi, K., Babashah, S., Sadeghizadeh, M., & Nasr‐Esfahani, M. H. (2017) · Journal of Cellular Biochemistry · doi:10.1002/jcb.26590 · Cited by 21 · see citing works
Sumsuzzman, D. M., Jin, Y., Choi, J., Yu, J., Lee, T. H., & Hong, Y. (2019) · Tumor Biology · doi:10.1177/1010428319892790 · Cited by 20 · see citing works
Gheit, R. E. A. E., Younis, R. L., El‐Saka, M. H., Emam, M. N., Soliman, N. A., El‐Sayed, R. M., Hafez, Y. M., AbuoHashish, N. A., Radwan, D. A., Khaled, H. E., et al. (2022) · Journal of Physiology and Biochemistry · doi:10.1007/s13105-022-00915-3 · Cited by 20 · see citing works
Gallo‐Villegas, J., & Calderón, J. C. (2023) · European Journal of Applied Physiology · doi:10.1007/s00421-022-05114-y · Cited by 20 · see citing works
Irisin modulates glucose metabolism and inhibits steroidogenesis in bovine granulosa cells
Daudon, M., Ramé, C., Price, C. A., & Dupont, J. (2023) · Reproduction · doi:10.1530/rep-22-0404 · Cited by 20 · see citing works
Brenmoehl, J., Ohde, D., Albrecht, E., Walz, C., Tuchscherer, A., & Hoeflich, A. (2016) · Journal of Comparative Physiology B · doi:10.1007/s00360-016-1036-7 · Cited by 19 · see citing works
Molecular Basis of Irisin Regulating the Effects of Exercise on Insulin Resistance
Lin, J., Liu, X., Zhou, Y., Zhu, B., Wang, Y., Cui, W., Peng, Y., Wang, B., Chen, Z., & Zhao, R. (2022) · Applied Sciences · doi:10.3390/app12125837 · Cited by 18 · see citing works
The role of Irisin in multiorgan protection
Ma, J., & Chen, K. (2021) · Molecular Biology Reports · doi:10.1007/s11033-020-06067-1 · Cited by 17 · see citing works
Petro, J. L., Fragozo-Ramos, M. C., Milán, A. F., Aristizábal, J. C., Gallo‐Villegas, J., & Calderón, J. C. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms24086874 · Cited by 17 · see citing works
Lee, Y. J., Heo, Y., Choi, J., Park, S., Kim, K. K., Shin, D. W., & Kang, J. (2019) · International Journal of Environmental Research and Public Health · doi:10.3390/ijerph16040660 · Cited by 17 · see citing works
Han, L., Li, P., He, Q., Yang, C. G., Jiang, M., Wang, Y., Cao, Y., Han, X., Liu, X., & Wu, W. (2023) · Aging and Disease · doi:10.14336/ad.2023.1125 · Cited by 16 · see citing works
Muñoz, I. Y. M., Camarillo-Romero, E. D. S., Padilla, T. C., Benítez, J. G. S., Romero, M. D. S. C., Morales, L. P. M., Bravo, G. G. H., & Garduño‐García, J. D. J. (2019) · Frontiers in Endocrinology · doi:10.3389/fendo.2019.00621 · Cited by 16 · see citing works
Abedi-Taleb, E., Vahabi, Z., Moghadam, E. S., Khedmat, L., Jazayeri, S., & Saboor-Yaraghi, A. A. (2019) · Lipids in Health and Disease · doi:10.1186/s12944-019-1128-y · Cited by 15 · see citing works
Irisin: linking metabolism with heart failure.
Li, J., Xie, S.-S., Guo, L., Jiang, J., & Chen, H. (2020) · PubMed Central · Cited by 15 · see citing works
Momenzadeh, S., Zamani, S., Tabrizi, Z. P. F., Barreiro, C., & Jami, M. (2021) · Biochimie · doi:10.1016/j.biochi.2021.06.016 · Cited by 14 · see citing works
Orioli, L., & Thissen, J. (2025) · Frontiers in Endocrinology · doi:10.3389/fendo.2025.1554617 · Cited by 13 · see citing works
Impacts of rat hindlimb Fndc5/irisin overexpression on muscle and adipose tissue metabolism
Farrash, W. F., Brook, M. S., Crossland, H., Phillips, B. E., Cegielski, J., Wilkinson, D. J., Constantin‐Teodosiu, D., Greenhaff, P. L., Smith, K., Cleasby, M. E., et al. (2020) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00034.2020 · Cited by 13 · see citing works
Huang, W., Liu, Y., Xu, H., Zhu, H., Guan, J., Yi, H., & Zou, J. (2020) · Endocrine Journal · doi:10.1507/endocrj.ej19-0590 · Cited by 13 · see citing works
Impact of diets rich in olive oil, palm oil or lard on myokine expression in rats
Gauze-Gnagne, C., Raynaud, F., Djohan, Y. F., Lauret, C., Feillet‐Coudray, C., Coudray, C., Monde, A., Koffi, G., Morena-Carrere, M., Camara-Cissé, M., et al. (2020) · Food & Function · doi:10.1039/d0fo01269f · Cited by 13 · see citing works
Zhang, Y., Wei, Q., Geng, X. H., & Fang, G. (2024) · Metabolites · doi:10.3390/metabo14120692 · Cited by 11 · see citing works
- Authors
- Vaughan, R. A., Gannon, N. P., Barberena, M. A., Garcia‐Smith, R., Bisoffi, M., Mermier, C. M., Conn, C. A., & Trujillo, K. A.
- Affiliations
- University of New Mexico (US)
- Corresponding author
- Vaughan, R. A.
- Venue
- Diabetes Obesity and Metabolism
- Publisher
- Wiley
- Published
- 2014-02-18
- Volume
- 16
- Issue
- 8
- Pages
- 711-718
- ISSN
- 1462-8902 (ISSN-L)
- 1463-1326
- OpenAlex
- W2000249408
- Cited by
- 150
- References
- 19
- Citations by year
- 2026: 7 · 2025: 11 · 2024: 11 · 2023: 10 · 2022: 15 · 2021: 15 · 2020: 14 · 2019: 15 · 2018: 5 · 2017: 14 · 2016: 9 · 2015: 15 · 2014: 9
- FWCI
- 10.41
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Diabetes Obesity and Metabolism (publishedVersion)
- PubMed (publishedVersion)
- Funders
- 2013 University of New Mexico- New Mexico Research Grant
- Topics
- Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
- Keywords
- irisin, skeletal muscle, C2C12 myocytes, mitochondrial biogenesis, mitochondrial uncoupling, oxidative metabolism, glycolysis, PGC-1α, NRF1, TFAM, GLUT4, UCP3, metabolic gene expression, mitochondrial content
- MeSH terms
- Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha, Animals, Cell Line, Cell Survival, DNA-Binding Proteins, Fibronectins, Gene Expression Regulation, Glycolysis, High Mobility Group Proteins, Humans, Kinetics, Mitochondria, Muscle, Muscle Proteins, Oxidative Phosphorylation, Recombinant Proteins, Transcription Factors, Muscle Fibers, Skeletal, Cell Proliferation, Nuclear Respiratory Factor 1, Mice, Mitochondrial Turnover
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