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Cited by 32Ion channel regulation and functionNicotinic Acetylcholine Receptors StudyNeuroscience and Neuropharmacology Research

Firing properties of Renshaw cells defined by Chrna2 are modulated by hyperpolarizing and small conductance ion currents Ih and ISK

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Perry, Sharn, Gezelius, Henrik, Larhammar, Martin, Hilscher, Markus M., Lamotte d'Incamps, Boris, Leao, Katarina E., Kullander, Klas·2015·European Journal of Neuroscience
DOI 10.1111/ejn.12852
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Abstract

Renshaw cells in the spinal cord ventral horn regulate motoneuron output through recurrent inhibition. Renshaw cells can be identified in vitro using anatomical and cellular criteria; however, their functional role in locomotion remains poorly defined because of the difficulty of functionally isolating Renshaw cells from surrounding motor circuits. Here we aimed to investigate whether the cholinergic nicotinic receptor alpha2 (Chrna2) can be used to identify Renshaw cells (RC s α2) in the mouse spinal cord. Immunohistochemistry and electrophysiological characterization of passive and active RC s α2 properties confirmed that neurons genetically marked by the Chrna2‐Cre mouse line together with a fluorescent reporter mouse line are Renshaw cells. Whole‐cell patch‐clamp recordings revealed that RC s α2 constitute an electrophysiologically stereotyped population with a resting membrane potential of −50.5 ± 0.4 mV and an input resistance of 233.1 ± 11 MΩ. We identified a ZD 7288‐sensitive hyperpolarization‐activated cation current (Ih) in all RC s α2, contributing to membrane repolarization but not to the resting membrane potential in neonatal mice. Additionally, we found RC s α2 to express small calcium‐activated potassium currents (ISK) that, when blocked by apamin, resulted in a complete attenuation of the afterhyperpolarisation potential, increasing cellular firing frequency. We conclude that RC s α2 can be genetically targeted through their selective Chrna2 expression and that they display currents known to modulate rebound excitation and firing frequency. The genetic identification of Renshaw cells and their electrophysiological profile is required for genetic and pharmacological manipulation as well as computational simulations with the aim to understand their functional role.

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  • Inhib-26 was the most robust ventral cluster and expressed the Renshaw marker genes Chrna2, Chrna7, and Calb1, suggesting it corresponded to Renshaw cells.

    “Inhib-26 was the most robust ventral cluster and expressed the Renshaw marker genes Chrna2, Chrna7, and Calb1, suggesting that this cluster corresponded to Renshaw cells89,90.”

    From A harmonized atlas of mouse spinal cord cell types and their spatial organization (2021) · cites this paper as [90]

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Authors
Perry, S., Gezelius, H., Larhammar, M., Hilscher, M. M., Lamotte d'Incamps, B., Leao, K. E., & Kullander, K.
Affiliations
  • Uppsala University (SE)
  • Neurophysique et Physiologie CNRS UMR 8119 Université Paris Descartes Paris France
  • Department of Neuroscience Uppsala University Box 593 751 24 Uppsala Sweden
ORCID
Venue
European Journal of Neuroscience
Publisher
Wiley
Published
2015-02-25
Volume
41
Issue
7
Pages
889-900
ISSN
  • 0953-816X (ISSN-L)
  • 1460-9568
DOI
10.1111/ejn.12852
OpenAlex
W2031933366
Cited by
32
References
66
Citations by year
2026: 2 · 2023: 3 · 2022: 5 · 2021: 4 · 2020: 1 · 2018: 9 · 2017: 5 · 2016: 3
FWCI
2.46
Type
journal article
Language
en
Open access
Licence
Locations
  • European Journal of Neuroscience (publishedVersion)
  • PubMed (publishedVersion)
  • LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (publishedVersion, other-oa)
  • Americanae (AECID Library) (submittedVersion, other-oa)
  • Figshare (submittedVersion, cc-by)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nicotinic Acetylcholine Receptors Study
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
Keywords
Renshaw cells, CHRNA2, spinal cord ventral horn, rebound excitation, firing rate, recurrent inhibition, motor neurons, apamin, locomotion
MeSH terms
Action Potentials, Animals, Animals, Newborn, Apamin, Ion Channels, Lumbar Vertebrae, Mice, Inbred C57BL, Mice, Transgenic, Motor Neurons, Pyrimidines, Receptors, Nicotinic, RNA, Messenger, Spinal Cord, Spinal Nerve Roots, Tissue Distribution, Neurotransmitter Agents, Mice, 129 Strain, Renshaw Cells

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