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Cited by 513D Printing in Biomedical ResearchCancer Cells and MetastasisCutaneous Melanoma Detection and Management

Progress in melanoma modelling in vitro

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Marconi, Alessandra, Quadri, Marika, Saltari, Annalisa, Pincelli, Carlo·2018·Experimental Dermatology
DOI 10.1111/exd.13670
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Abstract

Melanoma is one of the most studied neoplasia, although laboratory techniques used for investigating this tumor are not fully reliable. Animal models may not predict the human response due to differences in skin physiology and immunity. In addition, international guidelines recommend to develop processes that contribute to the reduction, refinement and replacement of animals for experiments (3Rs). Adherent cell culture has been widely used for the study of melanoma to obtain important information regarding melanoma biology. Nonetheless, these cells grow in adhesion on the culture substrate which differs considerably from the situation in vivo. Melanoma grows in a 3D spatial conformation where cells are subjected to a heterogeneous exposure to oxygen and nutrient. In addition, cell‐cell and cell‐matrix interaction play a crucial role in the pathobiology of the tumor as well as in the response to therapeutic agents. To better study, melanoma new techniques, including spherical models, tumorospheres and melanoma skin equivalents, have been developed. These 3D models allow to study tumors in a microenvironment that is more close to the in vivo situation and are less expensive and time‐consuming than animal studies. This review will also describe the new technologies applied to skin reconstructs such as organ‐on‐a‐chip that allows skin perfusion through microfluidic platforms. 3D in vitro models, based on the new technologies, are becoming more sophisticated, representing at a great extent the in vivo situation, the “perfect” model that will allow less involvement of animals up to their complete replacement, is still far from being achieved.

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  1. [1]1. K. Klimkiewicz, K. Weglarczyk, G. Collet, M. Paprocka, A. Guichard, M. Sarna, A. Jozkowicz, J. Dulak, T. Sarna, C. Grillon, C. Kieda, Cancer Lett. 2017, 396, 10.
  2. [2]2. H. Vörsmann, F. Groeber, H. Walles, S. Busch, S. Beissert, H. Walczak, D. Kulms, Cell Death Dis. 2013, 4, e719.
  3. [3]3. M. Sensi, G. Nicolini, C. Petti, I. Bersani, F. Lozupone, A. Molla, C. Vegetti, D. Nonaka, R. Mortarini, G. Parmiani, S. Fais, A. Anichini, Oncogene 2006, 25, 3357.
  4. [4]4. E. M. von Euw, M. M. Barrio, D. Furman, M. Bianchini, E. M. Levy, C. Yee, Y. Li, R. Wainstok, J. Mordoh, J. Transl. Med. 2007, 5, 19.
  5. [5]5. C. L. Slingluff Jr., T. A. Colella, L. Thompson, D. D. Graham, J. C. Skipper, J. Caldwell, L. Brinckerhoff, D. J. Kittlesen, D. H. Deacon, C. Oei, N. L. Harthun, E. L. Huczko, D. F. Hunt, T. L. Darrow, V. H. Engelhard, Cancer Immunol. Immunother. 2000, 48, 661.
  6. [6]6. V. A. Nikolaou, A. J. Stratigos, K. T. Flaherty, H. Tsao, J. Invest. Dermatol. 2012, 132, 854.
  7. [7]7. K. A. Beaumont, N. Mohana-Kumaran, N. K. Haass, Healthcare (Basel) 2013, 2, 27.
  8. [8]8. C. Khanna, K. Hunter, Carcinogenesis 2005, 26, 513.
  9. [9]9. G. Merlino, K. Flaherty, N. Acquavella, C. P. Day, A. Aplin, S. Holmen, S. Topalian, T. van Dyke, M. Herlyn, Pigment Cell Melanoma Res. 2013, 26, E8.
  10. [11]11. S. R. Kelsall, B. Mintz, Cancer Res. 1998, 58, 4061.
  11. [12]12. C. J. Kemp, Cold Spring Harb. Protoc. 2015, 10, 865.
  12. [13]13. J. Combest, P. J. Roberts, P. M. Dillon, K. Sandison, S. K. Hanna, C. Ross, S. Habibi, B. Zamboni, M. Müller, M. Brunner, N. E. Sharpless, W. C. Zamboni, Oncologist 2012, 17, 1303.
  13. [14]14. M. Śniegocka, E. Podgórska, P. M. Płonka, M. Elas, B. Romanowska- Dixon, M. Szczygieł, M. A. Żmijewski, M. Cichorek, A. Markiewicz, A. A. Brożyna, A. T. Słominski, K. Urbańska, Int. J. Mol. Sci. 2018, 19, 1048.
  14. [15]15. A. Slominski, R. Paus, Int. J. Oncol. 1993, 2, 221.
  15. [16]16. P. Pour, J. Althoff, S. Z. Salmasi, K. Stepan, J. Natl Cancer Inst. 1979, 63, 797.
  16. [17]17. M. Haldi, C. Ton, W. L. Seng, P. McGrath, Angiogenesis 2006, 9, 139.
  17. [18]18. K. Stoletov, V. Montel, R. D. Lester, S. L. Gonias, R. Klemke, Proc. Natl Acad. Sci. USA 2007, 104, 17406.
  18. [19]19. J. Seok, H. S. Warren, A. G. Cuenca, M. N. Mindrinos, H. V. Baker, W. Xu, D. R. Richards, G. P. McDonald-Smith, H. Gao, L. Hennessy, C. C. Finnerty, C. M. López, S. Honari, E. E. Moore, J. P. Minei, J. Cuschieri, P. E. Bankey, J. L. Johnson, J. Sperry, A. B. Nathens, T. R. Billiar, M. A. West, M. G. Jeschke, M. B. Klein, R. L. Gamelli, N. S. Gibran, B. H. Brownstein, C. Miller-Graziano, S. E. Calvano, P. H. Mason, J. P. Cobb, L. G. Rahme, S. F. Lowry, R. V. Maier, L. L. Moldawer, D. N. Herndon, R. W. Davis, W. Xiao, R. G. Tompkins, Inflammation and Host Response to Injury, Large Scale Collaborative Research Program, Proc. Natl Acad. Sci. USA 2013, 110, 3507.
  19. [20]20. I. W. Mak, N. Evaniew, M. Ghert, Am. J. Transl. Res. 2014, 6, 114.
  20. [21]21. M. L. Graham, M. J. Prescot, Eur. J. Pharmacol. 2015, 759, 19.
  21. [22]22. H. A. Neumann, H. M. Runge, H. H. Fiebig, R. Engelhardt, G. W. Löhr, J. Cancer Res. Clin. Oncol. 1986, 112, 165.
  22. [23]23. M. R. Carvalho, D. Lima, R. L. Reis, J. M. Oliveira, V. M. Correlo, Stem Cell Rev. 2017, 13, 347.
  23. [24]24. S. Huang, D. E. Ingber, Cancer Cell 2005, 8, 175.
  24. [25]25. F. Rambow, B. Job, V. Petit, F. Gesbert, V. Delmas, H. Seberg, G. Meurice, E. Van Otterloo, P. Dessen, C. Robert, D. Gautheret, R. A. Cornell, A. Sarasin, L. Larue, Cell Rep. 2015, 13, 840.
  25. [26]26. A. Malandrino, R. D. Kamm, E. Moeendarbary, ACS Biomater. Sci. Eng. 2018, 4, 294.
  26. [27]27. I. Serebriiskii, R. Castelló-Cros, A. Lamb, E. A. Golemis, E. Cukierman, Matrix Biol. 2008, 27, 573.
  27. [28]28. D. Loessner, K. S. Stok, M. P. Lutolf, D. W. Hutmacher, J. A. Clements, S. C. Rizzi, Biomaterials 2010, 31, 8494.
  28. [29]29. H. Karlsson, M. Fryknäs, R. Larsson, P. Nygren, Exp. Cell Res. 2012, 318, 1577.
  29. [30]30. R. M. Sutherland, J. A. McCredie, W. R. Inch, J. Natl Cancer Inst. 1971, 46, 113.
  30. [31]31. R. Z. Lin, H. Y. Chang, Biotechnol. J. 2008, 9, 1172.
  31. [32]32. L. A. Kunz-Schughart, J. P. Freyer, F. Hofstaedter, R. Ebner, J. Biomol. Screen. 2004, 9, 273.
  32. [33]33. N. A. Cody, M. Zietarska, A. Filali-Mouhim, D. M. Provencher, A. M. Mes-Masson, P. N. Tonin, BMC Med. Genomics 2008, 1, 34.
  33. [34]34. T. T. Chang, M. Hughes-Fulford, Tissue Eng. Part A 2009, 15, 55967.
  34. [36]36. A. Saltari, F. Truzzi, M. Quadri, R. Lotti, E. Palazzo, G. Grisendi, N. Tiso, A. Marconi, C. Pincelli, J. Invest. Dermatol. 2016, 136, 2049.
  35. [37]37. R. M. Sutherland, Science 1988, 240, 177.
  36. [38]38. V. Dangles-Marie, M. Pocard, S. Richon, L. B. Weiswald, F. Assayag, P. Saulnier, J. G. Juddle, J. L. Janneau, N. Auger, P. Validire, Cancer Res. 2007, 67, 398.
  37. [39]39. J. Friedrich, C. Seidel, R. Ebner, L. A. Kunz-Schughart, Nat. Protoc. 2009, 4, 309.
  38. [40]40. B. S. Youn, A. S. Sen, L. A. Behie, A. Girgis-Gabardo, J. A. Hassell, Biotechnol. Prog. 2006, 22, 801.
  39. [41]41. Y. Fang, R. M. Eglen, 3D Cell Cult. Drug Screen. Optim. 2017, 22, 456.
  40. [42]42. S. Sakai, K. Inamoto, Y. Liu, S. Tanaka, S. Arii, M. Taya, Cancer Sci. 2012, 103, 549.
  41. [43]43. S. Zhang, C. Balch, M. W. Chan, H. C. Lai, D. Matei, J. M. Schilder, P. S. Yan, T. H. Huang, K. P. Nephew, Cancer Res. 2008, 68, 4311.
  42. [44]44. K. Alessandri, B. R. Sarangi, V. V. Gurchenkov, B. Sinha, T. R. Kießling, L. Fetler, F. Rico, S. Scheuring, C. Lamaze, A. Simon, S. Geraldo, D. Vignjevic, H. Doméjean, L. Rolland, A. Funfak, J. Bibette, N. Bremond, P. Nassoy, Proc. Natl Acatd. Sci. U.S.A. 2013, 110, 14843.
  43. [45]45. S. Kellouche, J. Fernandes, J. Leroy Dudal, O. Gallet, S. Dutoit, L. Poulain, F. Carreiras, Tumor Biol. 2010, 31, 129.
  44. [46]46. R. Akasov, D. Zaytseva-Zotova, S. Burov, M. Leko, M. Dontenwill, M. Chiper, T. Vandamme, E. Markvicheva, Int. J. Pharm. 2016, 506, 1498.
  45. [47]47. J. Yang, M. Goto, H. Iso, C. S. Cho, T. Akaike, Biomaterials 2002, 23, 471.
  46. [48]48. E. T. Verjans, J. Doijen, W. Luyten, B. Landuyt, L. Schoofs, J. Cell. Physiol. 2018, 233, 2993.
  47. [49]49. X. Gong, C. Lin, J. Cheng, J. Su, H. Zhao, T. Liu, X. Wen, P. Zhao, PLoS ONE 2015, 10, 1371.
  48. [50]50. L. B. Weiswald, D. Bellet, V. Dangles-Marie, Neoplasia 2015, 17, 1.
  49. [51]51. F. Hirschhaeuser, M. Heike, C. Dittfeld, J. West, W. Mueller- Klieser, L. A. Kunz-Schughart, J. Biotechnol. 2010, 140, 3.
  50. [52]52. A. Raza, H. E. Colley, E. Baggaley, I. V. Sazanovich, N. H. Green, J. A. Weinstein, S. W. Botchway, S. MacNeil, J. W. Haycock, Sci. Rep. 2017, 7, 10743.
  51. [53]53. L. A. Kunz-Shugart, J. Doetsh, W. Muller-Klieser, K. Groebe, Am. J. Physiol. Cell Physiol. 2000, 278, C765.
  52. [54]54. D. F. Quail, J. T. Maciel, K. Rogers, L. M. Postovit, J. Biomol. Screen. 2012, 17, 1088.
  53. [55]55. K. L. Sodek, M. J. Ringuette, T. J. Brown, Int. J. Cancer 2009, 124, 2060.
  54. [56]56. B. Gole, M. B. Duran Alonso, V. Dolenc, T. Lah, Pathol. Oncol. Res. 2009, 15, 711.
  55. [57]57. S. Hauptmann, D. Budianto, C. Denkert, M. Kobel, L. Borsi, A. Siri, Oncology 2003, 65, 174.
  56. [58]58. O. De Wever, A. Hendrix, A. De Boeck, W. Westbroek, G. Breams, S. Emami, M. Sabbah, C. Gespach, M. Bracke, Int. J. Dev. Biol. 2010, 54, 887.
  57. [59]59. O. Oudar, Crit. Rev. Oncol. Hematol. 2000, 36, 99.
  58. [60]60. N. E. Timmins, S. Dietmair, L. K. Nielsen, Angiogenesis 2004, 7, 97.
  59. [61]61. B. Marrero, J. L. Messina, R. Heller, In Vitro Cell. Dev. Biol. Anim. 2009, 45, 523.
  60. [62]62. T. Shatton, M. K. Frank, J. Invest. Dermatol. 2010, 130, 1769.
  61. [63]63. T. Reya, S. J. Morrison, M. F. Clarke, I. L. Weissman, Nature 2001, 414, 105.
  62. [64]64. J. T. Lee, M. Herlyn, J. Cell. Physiol. 2007, 213, 603.
  63. [65]65. S. K. Singh, I. D. Clarke, T. Hide, P. B. Dirks, Oncogene 2004, 23, 7267.
  64. [66]66. D. Ponti, A. Costa, N. Zaffaroni, G. Pratesi, G. Petrangolini, D. Coradini, S. Pilotti, M. A. Pierotti, M. G. Daidone, Cancer Res. 2005, 65, 5506.
  65. [67]67. A. Eramo, F. Lotti, G. Sette, E. Pilozzi, M. Biffoni, A. Di Virgiglio, C. Conticello, L. Ruco, C. Peschle, R. De Maria, Cell Death Differ. 2008, 15, 504.
  66. [68]68. A. T. Collins, P. A. Berry, C. Hyde, M. J. Stower, N. J. Maitland, Cancer Res. 2005, 65, 10946.
  67. [69]69. M. Perego, M. Tortoreto, G. Tragni, L. Mariani, P. Deho, A. Carbone, M. Santinami, R. Patuzzo, P. D. Mina, A. Villa, G. Pratesi, G. Cossa, P. Perego, M. G. Daidone, M. R. Alison, G. Parmiani, L. Rivoltini, C. Castelli, J. Invest. Dermatol. 2010, 130, 1877.
  68. [70]70. B. A. Reynolds, S. Weiss, Science 1992, 255, 1707.
  69. [71]71. E. Monzani, F. Facchetti, E. Galmozzi, E. Corsini, A. Benetti, C. Cavazzin, A. Gritti, A. Piccinini, D. Porro, M. Santinami, G. Invernici, E. Parati, G. Alessandri, C. A. La Porta, Eur. J. Cancer 2007, 43, 935.
  70. [72]72. T. Schatton, G. F. Murphy, N. Y. Frank, K. Yamaura, A. M. Waaga- Gasser, M. Gasser, Q. Zhan, S. Jordan, L. M. Duncan, C. Weishaupt, R. C. Fuhlbrigge, T. S. Kupper, M. H. Sayegh, M. H. Frank, Nature 2008, 451, 345.
  71. [73]73. D. Fang, T. K. Nguyen, K. Leishear, R. Finko, A. N. Kulp, S. Hotz, P. A. Van Belle, X. Xu, D. E. Elder, M. Herlyn, Can. Res. 2005, 65, 9328.
  72. [74]74. J. Dou, M. Pan, P. Wen, Y. Li, Q. Tang, L. Chu, F. Zhao, C. Jiang, W. Hu, K. Hu, N. Gu, Cell. Mol. Immunol. 2007, 4, 467.
  73. [75]75. A. D. Boiko, O. V. Razorenova, M. van De Rijn, S. M. Swetter, D. L. Johnson, D. P. Ly, P. D. Butler, G. P. Yang, B. Joshua, M. J. Kaplan, M. T. Longaker, I. L. Weissman, Nature 2010, 466, 133.
  74. [76]76. M. F. Clarke, J. E. Dick, P. B. Dirks, C. J. Eaves, C. H. Jamieson, D. L. Jones, J. Visvader, I. L. Weissman, G. M. Wahl, Cancer Res. 2006, 66, 9339.
  75. [77]77. K. S. Smalley, M. Lioni, M. Herlyn, In Vitro Cell. Dev. Biol. Anim. 2006, 42, 242.
  76. [78]78. F. Meier, M. Nesbit, M.-Y. Hsu, B. Martin, P. Van Belle, D. E. Elder, G. Schaumburg-Lever, C. Garbe, T. M. Walz, P. Donatien, T. M. Crombleholme, M. Herlyn, Am. J. Pathol. 2000, 156, 1.
  77. [79]79. N. A. Bhowmick, E. G. Neilson, H. L. Moses, Nature 2004, 432, 332.
  78. [80]80. P. Micke, A. Ostman, Expert Opin. Ther. Targets. 2005, 9, 1217.
  79. [81]81. S. Dror, L. Sander, H. Schwartz, D. Sheinboim, A. Barzilai, Y. Dishon, S. Apcher, T. Golan, S. Greenberger, I. Barshack, H. Malcov, A. Zilberberg, L. Levin, M. Nessling, Y. Friedmann, V. Igras, O. Barzilay, H. Vaknine, R. Brenner, A. Zinger, A. Schroeder, P. Gonen, M. Khaled, N. Erez, J. D. Hoheisel, C. Levy, Nat. Cell Biol. 2016, 18, 1006.
  80. [83]83. C. A. Brohem, L. B. da Silva Cardeal, M. Tiago, M. S. Soengas, S. B. de Moraes Barros, S. S. Maria-Engler, Pigment Cell Melanoma Res. 2011, 24, 30.
  81. [84]84. P. Haridas, J. A. McGovern, S. D. L. McElwain, M. J. Simson, PeerJ 2017, 5, e3754.
  82. [85]85. M. Ponec, J. Kempenaar, J. Skin Pharmacol. 1995, 8, 499.
  83. [86]86. K. Jansson, G. Kratz, A. Haegerstrand, In Vitro Cell. Dev. Biol. Anim. 1996, 32, 53440.
  84. [87]87. K. Nakazawa, H. Nakazawa, F. Sahuc, A. Lepavec, C. Collombel, O. Damour, Pigment Cell Res. 1997, 10, 38290.
  85. [88]88. A. Slominski, D. J. Tobin, S. Shibahara, J. Wortsman, Physiol. Rev. 2004, 84, 1155.
  86. [89]89. R. A. Dawson, Z. Upton, J. Malda, D. G. Harkin, Transplantation 2006, 81, 1668.
  87. [90]90. D. S. Hill, N. D. P. Robinson, M. P. Caley, M. Chen, E. A. O'Toole, J. L. Armstrong, S. Przyborski, P. E. Lovat, Mol. Cancer Ther. 2015, 14, 2665.
  88. [91]91. W. H. Clark Jr., D. E. Elder, D. I. V. Guerry, M. N. Epstein, M. H. Greene, M. Van Horn, Hum. Pathol. 1984, 15, 1147.
  89. [92]92. J. Tsai, J. T. Lee, W. Wang, J. Zhang, H. Cho, S. Mamo, R. Bremer, S. Gillette, J. Kong, N. K. Haass, K. Sproesser, L. Li, K. S. Smalley, D. Fong, Y. L. Zhu, A. Marimuthu, H. Nguyen, B. Lam, J. Liu, I. Cheung, J. Rice, Y. Suzuki, C. Luu, C. Settachatgul, R. Shellooe, J. Cantwell, S. H. Kim, J. Schlessinger, K. Y. Zhang, B. L. West, B. Powell, G. Habets, C. Zhang, P. N. Ibrahim, P. Hirth, D. R. Artis, M. Herlyn, G. Bollag, Proc. Natl Acad. Sci. USA 2008, 105, 3041.
  90. [93]93. J. T. Lee, L. Li, P. A. Brafford, M. van den Eijnden, M. B. Halloran, K. Sproesser, N. K. Haass, K. S. Smalley, J. Tsai, G. Bollang, M. Herlyn, Pigment Cell Melanoma Res. 2010, 23, 820.
  91. [94]94. E. Bellas, M. Seiberg, J. Garlick, D. L. Kaplan, Macromol. Biosci. 2012, 12, 1627.
  92. [95]95. I. J. Kosten, S. W. Spiekstra, T. D. de Gruijl, S. Gibbs, Toxicol. Appl. Pharmacol. 2015, 287, 35.
  93. [96]96. N. Cubo, M. Garcia, J. F. Del Cañizo, D. Velasco, J. L. Jorcano, Biofabrication 2016, 9, 015006.
  94. [97]97. H. Cui, M. Nowicki, J. P. Fisher, L. G. Zhang, Adv. Healthc. Mater. 2017, 6, 1.
  95. [98]98. S. N. Bhatia, D. E. Ingber, Nat. Biotechnol. 2014, 32, 760.
  96. [99]99. A. Pavesi, G. Adriani, A. Tay, M. E. Warkiani, W. H. Yeap, S. C. Wong, R. D. Kamm, Sci. Rep. 2016, 24, 26584.
  97. [100]100. H. E. Abaci, Z. Guo, Y. Doucet, J. Jacków, A. Christiano, Exp. Biol. Med. (Maywood). 2017, 242, 1657.
  98. [101]101. N. Mori, Y. Morimoto, S. Takeuchi, Biomaterials 2017, 116, 48.
  99. [102]102. H. E. Abaci, K. Gledhill, Z. Guo, A. M. Christiano, M. L. Shuler, Lab Chip 2015, 15, 882.
  100. [103]103. H. J. Pandya, K. Dhingra, D. Prabhakar, V. Chandrasekar, S. K. Natarajan, A. S. Vasan, A. Kulkarni, H. Shafiee, Biosens. Bioelectron. 2017, 94, 632.
  101. [104]104. F. Mattei, G. Schiavoni, A. De Ninno, V. Lucarini, P. Sestili, A. Sistigu, A. Fragale, M. Sanchez, M. Spada, A. Gerardino, F. Belardelli, L. Businaro, L. Gabriele, J. Immunotoxicol. 2014, 11, 337.

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Authors
Marconi, A., Quadri, M., Saltari, A., & Pincelli, C.
Affiliations
University of Modena and Reggio Emilia (IT)
Corresponding author
Marconi, Alessandra
ORCID
Venue
Experimental Dermatology
Publisher
Wiley
Published
2018-05
Volume
27
Issue
5
Pages
578-586
ISSN
  • 0906-6705 (ISSN-L)
  • 1600-0625
DOI
10.1111/exd.13670
OpenAlex
W2802665732
Cited by
51
References
100
Citations by year
2026: 4 · 2025: 4 · 2024: 6 · 2023: 11 · 2022: 9 · 2021: 7 · 2020: 6 · 2019: 2 · 2018: 2
FWCI
0.35
Type
journal article
Language
en
Open access
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor, 36 d)
Locations
  • Experimental Dermatology (publishedVersion)
  • PubMed (publishedVersion)
  • IRIS UNIMORE (University of Modena and Reggio Emilia) (submittedVersion, cc-by)
Topics
  • Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
  • Health Sciences › Medicine › Oncology › Cancer Cells and Metastasis
  • Health Sciences › Medicine › Oncology › Cutaneous Melanoma Detection and Management
Keywords
melanoma, in vitro model, 3D tumor models, organ-on-a-chip, microfluidics, 3Rs, animal model limitations, cell-matrix interactions, cell-cell interactions, tumor microenvironment
MeSH terms
Humans, Melanoma, Models, Biological, Cell Culture Techniques

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