The chemokine receptor CCR1 is strongly up‐regulated after skin injury but dispensable for wound healing
Abstract
To identify key regulators of cutaneous wound repair, we analyzed a subtractive cDNA library of normal and wounded mouse skin. One of the identified genes encodes the chemokine receptor CCR1, which binds several chemokines present at the wound site. Expression of CCR1 was barely detectable in nonwounded skin, but strong up‐regulation was observed after injury to wild‐type mice. Most important, the healing abnormalities observed in glucocorticoid‐treated mice and activin‐overexpressing transgenic mice correlated with an altered expression of CCR1. CCR1‐positive cells were identified as macrophages and neutrophils within the wounded area. To determine the importance of CCR1 for wound repair, we analyzed this process in CCR1 knockout mice. Surprisingly, no alterations in either wound closure, wound appearance, wound bursting strength, granulation tissue formation, or re‐epithelialization were observed. In addition, the inflammatory response was unaltered in CCR1‐deficient mice and the expression of several chemokines, chemokine receptors, and other important regulators of wound repair were normal in these animals. These results show that CCR1 is dispensable for wound healing, most likely due to redundancy in chemokine/chemokine receptor signaling.
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The CCR1−/− mouse, in which leukocyte recruitment is impaired, shows no change in wound healing in experimental acute wounds.
“The CCR1−/− mouse, in which leukocyte recruitment is impaired, shows no change in wound healing in experimental acute wounds.51”
From The Innate Immune System in Acute and Chronic Wounds (2016) · cites this paper as [51]
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“The CCR1 - / mouse, in which leukocyte recruitment is impaired, shows no change in wound healing in experimental acute wounds.(51) The disparity between the expected importance of a major innate immune system component and its dispensability for healing is thought to reflect the differing roles of neutrophils in the n…”
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- Authors
- Kaesler, S., Bugnon, P., Gao, J., Murphy, P. M., Goppelt, A., & Werner, S.
- Affiliations
- National Institutes of Health (US)
- Corresponding author
- Werner, Sabine
- Venue
- Wound Repair and Regeneration
- Publisher
- Wiley
- Published
- 2004-04
- Volume
- 12
- Issue
- 2
- Pages
- 193-204
- ISSN
- 1067-1927 (ISSN-L)
- 1524-475X
- OpenAlex
- W2002191327
- Cited by
- 50
- References
- 55
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 1 · 2023: 1 · 2022: 2 · 2020: 4 · 2019: 1 · 2018: 2 · 2017: 4 · 2016: 3 · 2015: 1 · 2014: 2 · 2013: 1 · 2012: 3
- FWCI
- 5.46
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Wound Repair and Regeneration (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Rehabilitation › Wound Healing and Treatments
- Health Sciences › Medicine › Oncology › Chemokine receptors and signaling
- Health Sciences › Medicine › Hepatology › Liver physiology and pathology
- Keywords
- CCR1, chemokine receptors, skin wound healing, skin damage, glucocorticoid therapy, activin, macrophages, neutrophils, wound closure, re-epithelialization, granulation tissue, wound inflammation
- MeSH terms
- Animals, Carbon Tetrachloride, Liver Cirrhosis, Male, Mice, Inbred BALB C, RNA, Messenger, Down-Regulation, Gene Expression, Transforming Growth Factor beta, Oligonucleotides, Antisense, Immediate-Early Proteins, Models, Animal, Collagen Type I, Intercellular Signaling Peptides and Proteins, Mice, Transforming Growth Factor beta1, Connective Tissue Growth Factor
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