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Cited by 104Microbial Metabolites in Food BiotechnologyGut microbiota and healthProbiotics and Fermented Foods

Baseline microbiota activity and initial bifidobacteria counts influence responses to prebiotic dosing in healthy subjects

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DE PRETER, V., VANHOUTTE, T., HUYS, G., SWINGS, J., RUTGEERTS, P., VERBEKE, K.·2008·Alimentary Pharmacology & Therapeutics
DOI 10.1111/j.1365-2036.2007.03588.x
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Abstract

Summary: Background Dietary intervention with prebiotics can cause changes in the colonic microbiota and their metabolic activities. Aim To investigate whether the response to prebiotic dosing is influenced by the baseline metabolic activity of the colonic flora and bifidobacteria counts. Methods The 4‐week effect of lactulose (10 g bid.; n = 29) and oligofructose‐enriched inulin (10 g bid.; n = 19) was evaluated in healthy human volunteers. Lactose‐[15 N, 15 N]‐ureide was used to study the colonic NH 3‐metabolism. Urine (48 h) and faeces (72 h) were collected and analysed for p‐cresol and 15 N‐content by gas chromatography–mass spectrometry and isotope ratio mass spectrometer, respectively. Faecal bifidobacteria were quantified by real‐time polymerase chain reaction. Results After the 4‐week prebiotic administration period, the urinary excretion of p‐cresol and 15 N was significantly decreased in both groups (P < 0.05) corresponding to a significantly higher faecal excretion of 15 N (P < 0.05). The decrease in urinary 15 N and p‐cresol excretion significantly correlated with baseline 15 N and p‐cresol levels (P < 0.05), indicating that subjects with higher baseline levels showed a higher response to prebiotic dosing. Furthermore, a significant correlation was seen between baseline bifidobacteria counts and the effect of prebiotic intake (P < 0.05). Conclusion The response to prebiotic dosing, as indicated by the fate of NH 3, p‐cresol and bifidobacteria, is determined by the initial colonic conditions.

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  • Inulin can stimulate the growth of Bifidobacterium spp. and Faecalibacterium prausnitzii, while increasing butyrate production.

    “Numerous studies have shown that inulin can stimulate the growth of Bifidobacterium spp.and Faecalibacterium prausnitzii, while increasing butyrate production (de Preter et al., 2008;Kolida et al., 2007;Ramirez-Farias et al., 2009).”

    From The Microbiota-Gut-Brain Axis (2019) · cites this paper as de Preter et al., 2008

    The Discussion (page unavailable in our copy) reports that oligofructose-enriched inulin increased faecal bifidobacteria in healthy volunteers. The Methods identify this substrate as a mixture containing inulin. The paper does not report growth of Faecalibacterium prausnitzii or an increase in butyrate production; its reference to fermentation producing short-chain fatty acids does not establish that specific result. (LLM, conf=0.88)

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Authors
DE PRETER, V., VANHOUTTE, T., HUYS, G., SWINGS, J., RUTGEERTS, P., & VERBEKE, K.
Affiliations
  • Ghent University (BE)
  • Universitair Ziekenhuis Leuven (BE)
Corresponding author
VERBEKE, K.
ORCID
Venue
Alimentary Pharmacology & Therapeutics
Publisher
Wiley
Published
2007-12-10
Volume
27
Issue
6
Pages
504-513
ISSN
  • 0269-2813 (ISSN-L)
  • 1365-2036
DOI
10.1111/j.1365-2036.2007.03588.x
OpenAlex
W1873092804
Cited by
104
References
0
Citations by year
2026: 2 · 2025: 3 · 2024: 7 · 2023: 4 · 2022: 3 · 2021: 6 · 2020: 9 · 2019: 10 · 2018: 9 · 2017: 8 · 2016: 6 · 2015: 4 · 2014: 7 · 2013: 6 · 2012: 3
FWCI
4.31
Type
journal article
Language
en
Open access
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor)
Locations
  • Alimentary Pharmacology & Therapeutics (publishedVersion)
  • PubMed (publishedVersion)
Funders
Vlaamse regering
Topics
  • Health Sciences › Nursing › Nutrition and Dietetics › Microbial Metabolites in Food Biotechnology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
Keywords
prebiotics, colonic microbiota, Bifidobacterium, lactulose, oligofructose-enriched inulin, p-cresol, urinary excretion, fecal excretion, 15N-urea, ammonia metabolism, healthy volunteers
MeSH terms
Adult, Bifidobacterium, Colon, Dietary Carbohydrates, Feces, Female, Gastrointestinal Transit, Humans, Male, Colony Count, Microbial, Probiotics

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