The DOI matches its Crossref record; 42 of the 42 references it cites were found in a registry or an index.
Serum testosterone, dihydrotestosterone and estradiol concentrations in older men self-reporting very good health: The healthy man study
Abstract
Summary: Objective: To determine serum concentrations, intra‐individual variability and impact of age‐related co‐morbidities on serum testosterone (T), dihydrotestosterone (DHT), estradiol (E 2) and estrone (E 1) in older men. Design: Observational, repeated measures study. Participants: Men (n = 325) with 40 years and older self‐reporting very good or excellent health. Measurements: Standardized history, physical examination and collection of nine blood samples at fixed time intervals were measured over 3 months (three at 20 min intervals on days 1 (fasting) and 2 (non‐fasting), one at days 7, 30 and 90). Serum T, DHT, E 2 and E 1 (n = 2900, > 99% of scheduled samples) measured by liquid chromatography‐tandem mass spectrometry (LC‐MS) were analysed by linear mixed model analysis with fasting, age and obesity as covariables. Results: Mean serum T did not vary with age (P = 0·76) but obesity (−0·35 n m per body mass index (BMI) unit, P < 0·0001) and ex‐smoker status (−1·6 n m, P < 0·001) had significant effects. Serum DHT was increased with age (+0·011 n m per year, P = 0·001) but decreased with obesity (−0·05 n m per BMI unit, P < 0·0001). Serum E 2 did not vary with age (P = 0·31) or obesity (P = 0·12). Overnight fasting increased (by 9–16%, all P < 0·001) and reduced variability in morning serum T, DHT, E 2 and E 1. Non‐fasting serum T and DHT were stable over time (day, week, month or 3 months; P > 0·28). Conclusions: Serum T, DHT and E 2 displayed no decrease associated with age among men over 40 years of age who self‐report very good or excellent health although obesity and ex‐smoking status were associated with decreased serum androgens (T and DHT) but not E 2. These findings support the interpretation that the age‐related decline in blood T accompanying non‐specific symptoms in older men may be due to accumulating age‐related co‐morbidities rather than a symptomatic androgen deficiency state.
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In the citing paper
“Conversely, there is increasing evidence that healthy ageing by itself is uncommonly associated with marked reductions in testosterone levels.23”
From Lowered testosterone in male obesity: Mechanisms, morbidity and management (2014) · cites this paper as [23]
In the citing paper
“Early studies comparing young (mean age 25–27 years) versus elderly males (mean age 71 years) demonstrated peak T concentrations in the 3–8 AM time period (extended to 2 PM in elderly males), with trough levels noted in the early afternoon to late evening.”
From Challenges in Testosterone Measurement, Data Interpretation, and Methodological Appraisal of Interventional Trials (2016) · cites this paper as [43]
In the citing paper
“T levels exhibit time, sleep, and age-dependent circadian variations.”
From Challenges in Testosterone Measurement, Data Interpretation, and Methodological Appraisal of Interventional Trials (2016) · cites this paper as [43]
In the citing paper
“Despite similar patterns of variation throughout the day, elderly males experienced significant blunting of peak versus trough levels, with differences in high and low values of 61–205 ng/dl (2.1–7.1 nmol/L) compared to 141–354 ng/dl (4.9–12.3 nmol/L) in younger men.”
From Challenges in Testosterone Measurement, Data Interpretation, and Methodological Appraisal of Interventional Trials (2016) · cites this paper as [43]
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Metadata
- Authors
- Sartorius, G., Spasevska, S., Idan, A., Turner, L., Forbes, E., Zamojska, A., Allan, C. A., Ly, L. P., Conway, A. J., McLachlan, R. I., & Handelsman, D. J.
- Affiliations
- The University of Sydney (AU)
- Concord Hospital (US)
- Prince Henrys Institute Clayton
- Corresponding author
- Handelsman, David J.
- ORCID
- Venue
- Clinical Endocrinology
- Publisher
- Wiley
- Published
- 2012-10-05
- Volume
- 77
- Issue
- 5
- Pages
- 755-763
- ISSN
- 0300-0664 (ISSN-L)
- 1365-2265
- OpenAlex
- W1984060669
- Cited by
- 209
- References
- 41
- Citations by year
- 2026: 6 · 2025: 4 · 2024: 5 · 2023: 24 · 2022: 9 · 2021: 10 · 2020: 14 · 2019: 14 · 2018: 12 · 2017: 16 · 2016: 26 · 2015: 23 · 2014: 23 · 2013: 18 · 2012: 4
- FWCI
- 17.45
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Clinical Endocrinology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Bupa Foundation
- Bupa Health Foundation
- Centers for Disease Control and Prevention
- Topics
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Hormonal and reproductive studies
- Health Sciences › Medicine › Reproductive Medicine › Sperm and Testicular Function
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Adrenal Hormones and Disorders
- Keywords
- serum testosterone, dihydrotestosterone, estradiol, estrone, older men, aging, obesity, smoking, fasting effects, diurnal variation, androgen levels, healthy aging
- MeSH terms
- Aged, Estradiol, Humans, Male, Middle Aged, Dihydrotestosterone, Testosterone
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