Detection and identification of Bartonella sp. in fleas from carnivorous mammals in Andalusia, Spain
Abstract
Abstract.: A total of 559 fleas representing four species ( Pulex irritans, Ctenocephalides felis, Ctenocephalides canis and Spilopsyllus cuniculi ) collected on carnivores (five Iberian lynx Lynx pardinus , six European wildcat Felis silvestris , 10 common genet Genetta genetta , three Eurasian badger Meles meles , 22 red fox Vulpes vulpes , 87 dogs and 23 cats) in Andalusia, southern Spain, were distributed in 156 pools of monospecific flea from each carnivore, and tested for Bartonella infection in an assay based on polymerase chain reaction (PCR) amplification of the 16 S–23 S rRNA intergenic spacer region. Twenty‐one samples (13.5%) were positive and the sequence data showed the presence of four different Bartonella species. Bartonella henselae was detected in nine pools of Ctenocephalides felis from cats and dogs and in three pools of Ctenocephalides canis from cats; Bartonella clarridgeiae in Ctenocephalides felis from a cat, and Bartonella alsatica in Spilopsyllus cuniculi from a wildcat. DNA of Bartonella sp., closely related to Bartonella rochalimae , was found in seven pools of Pulex irritans from foxes. This is the first detection of B. alsatica and Bartonella sp. in the Iberian Peninsula. All of these Bartonella species have been implicated as agents of human diseases. The present survey confirms that carnivores are major reservoirs for Bartonella spp.
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Both B. henselae and B. clarridgeiae have been found in fleas collected from dogs and cats in France and Germany, Albania, Spain, the Netherlands, Hungary, UK, and in the USA.
“As key examples, both B. henselae and B. clarridgeiae have been found in fleas collected from dogs and cats in France and Germany [161], Albania [162], Spain [90], the Netherlands [98], Hungary [163], UK [159], and in the USA as well [164].”
From Fleas infesting pets in the era of emerging extra-intestinal nematodes (2013) · cites this paper as [90]
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- Authors
- MÁRQUEZ, F. J., MILLÁN, J., RODRÍGUEZ‐LIÉBANA, J. J., GARCÍA‐EGEA, I., & MUNIAIN, M. A.
- Affiliations
- Universidad de Jaén (ES)
- Consejo Superior de Investigaciones Científicas (ES)
- Hospital Universitario Virgen Macarena (ES)
- Corresponding author
- MÁRQUEZ, F. J.
- Venue
- Medical and Veterinary Entomology
- Publisher
- Wiley
- Published
- 2009-11-17
- Volume
- 23
- Issue
- 4
- Pages
- 393-398
- ISSN
- 0269-283X (ISSN-L)
- 1365-2915
- OpenAlex
- W2022187405
- Cited by
- 61
- References
- 38
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 4 · 2023: 3 · 2022: 5 · 2021: 6 · 2020: 5 · 2019: 2 · 2018: 4 · 2017: 2 · 2016: 3 · 2015: 6 · 2014: 2 · 2013: 3 · 2012: 2
- FWCI
- 2.87
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Medical and Veterinary Entomology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Junta de Andalucía
- Topics
- Life Sciences › Immunology and Microbiology › Parasitology › Bartonella species infections research
- Life Sciences › Immunology and Microbiology › Parasitology › Vector-borne infectious diseases
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacillus and Francisella bacterial research
- Keywords
- Bartonella, fleas, zoonotic reservoirs, Bartonella henselae, Bartonella clarridgeiae, Bartonella rochalimae, Ctenocephalides felis, Ctenocephalides canis, Spilopsyllus cuniculi, Pulex irritans, Iberian lynx, European wildcat, common genet, Meles meles, red fox, dogs, cats, 16S-23S rRNA intergenic spacer, Andalusia
- MeSH terms
- Animals, Bartonella, Bartonella Infections, Base Sequence, Carnivora, Disease Reservoirs, DNA, Bacterial, Siphonaptera, Molecular Sequence Data, Phylogeny, Spain, Polymerase Chain Reaction, Sequence Alignment, DNA, Ribosomal Spacer
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