The DOI matches its Crossref record; 38 of the 38 references it cites were found in a registry or an index.
Large‐scale insertional mutagenesis using the Tnt1 retrotransposon in the model legume Medicago truncatula
Abstract
Summary: Medicago truncatula is a fast‐emerging model for the study of legume functional biology. We used the tobacco retrotransposon Tnt1 to tag the Medicago genome and generated over 7600 independent lines representing an estimated 190 000 insertion events. Tnt1 inserted on average at 25 different locations per genome during tissue culture, and insertions were stable during subsequent generations in soil. Analysis of 2461 Tnt1 flanking sequence tags (FSTs) revealed that Tnt1 appears to prefer gene‐rich regions. The proportion of Tnt1 insertion in coding sequences was 34.1%, compared to the expected 15.9% if random insertions were to occur. However, Tnt1 showed neither unique target site specificity nor strong insertion hot spots, although some genes were more frequently tagged than others. Forward‐genetic screening of 3237 R 1 lines resulted in identification of visible mutant phenotypes in approximately 30% of the regenerated lines. Tagging efficiency appears to be high, as all of the 20 mutants examined so far were found to be tagged. Taking the properties of Tnt1 into account and assuming 1.7 kb for the average M. truncatula gene size, we estimate that approximately 14 000–16 000 lines would be sufficient for 90% gene tagging coverage in M. truncatula. This is in contrast to more than 500 000 lines required to achieve the same saturation level using T‐DNA tagging. Our data demonstrate that Tnt1 is an efficient insertional mutagen in M. truncatula, and could be a primary choice for other plant species with large genomes.
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Cites
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Genome-Wide Insertional Mutagenesis of Arabidopsis thaliana
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Nested Retrotransposons in the Intergenic Regions of the Maize Genome
SanMiguel, P., Tikhonov, A., Jin, Y.-K., Motchoulskaia, N., Zakharov, D., Melake-Berhan, A., Springer, P. S., Edwards, K. J., Lee, M., Avramova, Z., et al. (1996) · Science · doi:10.1126/science.274.5288.765 · Cited by 1,401
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T-DNA as an Insertional Mutagen in Arabidopsis
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Medicago truncatula — a model in the making!
Cook, D. R. (1999) · Current Opinion in Plant Biology · doi:10.1016/s1369-5266(99)80053-3 · Cited by 567
Miyao, A., Tanaka, K., Murata, K., Sawaki, H., Takeda, S., Abe, K., Shinozuka, Y., Onosato, K., & Hirochika, H. (2003) · The Plant Cell · doi:10.1105/tpc.012559 · Cited by 518
Cited by
Gene Editing and Crop Improvement Using CRISPR-Cas9 System
Arora, L., & Narula, A. (2017) · Frontiers in Plant Science · doi:10.3389/fpls.2017.01932 · Cited by 367
Invasion by Invitation: Rhizobial Infection in Legumes
Murray, J. D. (2011) · Molecular Plant-Microbe Interactions® · doi:10.1094/mpmi-08-10-0181 · Cited by 245
Couzigou, J.-M., Zhukov, V., Mondy, S., Abu el Heba, G., Cosson, V., Ellis, T. H. N., Ambrose, M., Wen, J., Tadege, M., Tikhonovich, I., et al. (2012) · The Plant Cell · doi:10.1105/tpc.112.103747 · Cited by 145
Self-citationCheng, X., Peng, J., Ma, J., Tang, Y., Chen, R., Mysore, K. S., & Wen, J. (2012) · New Phytologist · doi:10.1111/j.1469-8137.2012.04147.x · Cited by 93
Self-citationConserved genetic determinant of motor organ identity in Medicago truncatula and related legumes
Chen, J., Moreau, C., Liu, Y., Kawaguchi, M., Hofer, J., Ellis, N., & Chen, R. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1204566109 · Cited by 67
Metadata
- Authors
- Tadege, M., Wen, J., He, J., Tu, H., Kwak, Y., Eschstruth, A., Cayrel, A., Endre, G., Zhao, P. X., Chabaud, M., Ratet, P., & Mysore, K. S.
- Venue
- The Plant Journal
- Publisher
- Wiley
- Published
- 2008-01-16
- Volume
- 54
- Issue
- 2
- Pages
- 335-347
- ISSN
- 0960-7412 (ISSN-L)
- 1365-313X
- OpenAlex
- W2160656965
- Cited by
- 486
- References
- 38
- FWCI
- 28.54
- Type
- journal article
- Language
- en
- Open access
- closed
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- Tadege, M., Wen, J., He, J., Tu, H., Kwak, Y., Eschstruth, A., Cayrel, A., Endre, G., Zhao, P. X., Chabaud, M., Ratet, P., & Mysore, K. S. (2008). Large‐scale insertional mutagenesis using the Tnt1 retrotransposon in the model legume Medicago truncatula. The Plant Journal. https://doi.org/10.1111/j.1365-313x.2008.03418.x
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