The DOI matches its Crossref record; 45 of the 46 references it cites were found in a registry or an index.
MTP1-dependent Zn sequestration into shoot vacuoles suggests dual roles in Zn tolerance and accumulation in Zn-hyperaccumulating plants
Open accessAbstract
The integral membrane protein Thlaspi goesingense metal tolerance protein 1 (TgMTP1) has been suggested to play an important role in Zn hyperaccumulation in T. goesingense. Here, we show that the TgMTP1 protein is accumulated to high levels at the vacuolar membrane in shoot tissue of T. goesingense. TgMTP1 is likely to act in the transport of Zn into the vacuole, enhancing both Zn accumulation and tolerance. By specifically expressing TgMTP1 in Arabidopsis thaliana shoots, we show that TgMTP1, localized at the vacuolar membrane, can drive the enhanced shoot accumulation of Zn by initiating a systemic Zn deficiency response. The systematic response includes increased expression of Zn transporters (ZIP3, ZIP4, ZIP5 and ZIP9) in both shoot and root tissue. Furthermore, shoot-specific accumulation of TgMTP1 at the vacuolar membrane also leads to increased resistance to Zn in A. thaliana, probably through enhanced Zn compartmentalization in the vacuole. Such evidence leads to the conclusion that the high levels of TgMTP1 at the vacuolar membrane in shoot tissue of the Zn hyperaccumulator T. goesingense play a role in both Zn tolerance and enhanced Zn uptake and accumulation, via the activation of a systemic Zn deficiency response.
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“Moreover, MTP1 was overexpressed in A. halleri and N. caerulescens to transport Zn and Cd (Dräger et al., 2004; Gustin et al., 2009).”
From Plant hyperaccumulators: a state-of-the-art review on mechanism of heavy metal transport and sequestration (2025) · cites this paper as Gustin et al., 2009
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“The transformation of TgMTP1 in A. thaliana resulted in enhanced accumulation of Zn into vacuoles, attributing to Zn tolerance (Gustin et al., 2009).”
From Plant hyperaccumulators: a state-of-the-art review on mechanism of heavy metal transport and sequestration (2025) · cites this paper as Gustin et al., 2009
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Cites
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Self-citationEvolution of metal hyperaccumulation required cis-regulatory changes and triplication of HMA4
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Rascio, N., & Navari-Izzo, F. (2011) · Plant Science · doi:10.1016/j.plantsci.2010.08.016 · Cited by 2,012
“sion of ZIP transporters in hyperaccumulator plants [105].”
BackgroundMetal hyperaccumulation in plants
Krämer, U. (2010) · Annual Review of Plant Biology · doi:10.1146/annurev-arplant-042809-112156 · Cited by 1,284
The molecular mechanism of zinc and cadmium stress response in plants
Lin, Y., & Aarts, M. G. M. (2012) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-012-1089-z · Cited by 689
Facing the challenges of Cu, Fe and Zn homeostasis in plants
Palmer, C. M., & Guerinot, M. L. (2009) · Nature Chemical Biology · doi:10.1038/nchembio.166 · Cited by 633
Approaches for enhanced phytoextraction of heavy metals
Bhargava, A., Carmona, F. F. F., Bhargava, M., & Srivastava, S. (2012) · Journal of Environmental Management · doi:10.1016/j.jenvman.2012.04.002 · Cited by 585
Metadata
- Authors
- Gustin, J. L., Loureiro, M. E., Kim, D., Na, G., Tikhonova, M., & Salt, D. E.
- Affiliations
- Purdue University West Lafayette (US)
- Universidade Federal de Viçosa (BR)
- Gyeongsang National University (KR)
- Corresponding author
- Salt, David E.
- ORCID
- Gustin, Jeffery L.
- Venue
- The Plant Journal
- Publisher
- Wiley
- Published
- 2009-03
- Volume
- 57
- Issue
- 6
- Pages
- 1116-1127
- ISSN
- 0960-7412 (ISSN-L)
- 1365-313X
- OpenAlex
- W2119893617
- Cited by
- 203
- References
- 46
- Citations by year
- 2026: 7 · 2025: 7 · 2024: 7 · 2023: 8 · 2022: 4 · 2021: 24 · 2020: 18 · 2019: 15 · 2018: 8 · 2017: 10 · 2016: 12 · 2015: 8 · 2014: 13 · 2013: 14 · 2012: 11
- FWCI
- 14.92
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- The Plant Journal (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Science Foundation
- Purdue University
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Micronutrient Interactions and Effects
- Health Sciences › Nursing › Nutrition and Dietetics › Trace Elements in Health
- Physical Sciences › Chemistry › Analytical Chemistry › Heavy Metals in Plants
- Keywords
- metal tolerance proteins, zinc hyperaccumulation, tonoplast, zinc tolerance, ZIP transporters, zinc uptake, Arabidopsis thaliana transformation, metal hyperaccumulator plants
- MeSH terms
- Amino Acid Sequence, Molecular Sequence Data, Plant Proteins, Vacuoles, Zinc, Sequence Alignment, Arabidopsis, Gene Expression Regulation, Plant, Plant Shoots, Cation Transport Proteins, Thlaspi
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