Zinc-Dependent Global Transcriptional Control, Transcriptional Deregulation, and Higher Gene Copy Number for Genes in Metal Homeostasis of the Hyperaccumulator Arabidopsis halleri

Talke, Ina N.; Hanikenne, Marc; Krämer, Ute

doi:10.1104/pp.105.076232

Cited by 452Plant Micronutrient Interactions and EffectsHeavy metals in environmentHeavy Metals in Plants

Zinc-Dependent Global Transcriptional Control, Transcriptional Deregulation, and Higher Gene Copy Number for Genes in Metal Homeostasis of the Hyperaccumulator Arabidopsis halleri

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Talke, Ina N., Hanikenne, Marc, Krämer, Ute·2006·Plant Physiology

DOI 10.1104/pp.105.076232PMC PMC1557598

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Abstract

The metal hyperaccumulator Arabidopsis halleri exhibits naturally selected zinc (Zn) and cadmium (Cd) hypertolerance and accumulates extraordinarily high Zn concentrations in its leaves. With these extreme physiological traits, A. halleri phylogenetically belongs to the sister clade of Arabidopsis thaliana. Using a combination of genome-wide cross species microarray analysis and real-time reverse transcription-PCR, a set of candidate genes is identified for Zn hyperaccumulation, Zn and Cd hypertolerance, and the adjustment of micronutrient homeostasis in A. halleri. Eighteen putative metal homeostasis genes are newly identified to be more highly expressed in A. halleri than in A. thaliana, and 11 previously identified candidate genes are confirmed. The encoded proteins include HMA4, known to contribute to root-shoot transport of Zn in A. thaliana. Expression of either AtHMA4 or AhHMA4 confers cellular Zn and Cd tolerance to yeast (Saccharomyces cerevisiae). Among further newly implicated proteins are IRT3 and ZIP10, which have been proposed to contribute to cytoplasmic Zn influx, and FRD3 required for iron partitioning in A. thaliana. In A. halleri, the presence of more than a single genomic copy is a hallmark of several highly expressed candidate genes with possible roles in metal hyperaccumulation and metal hypertolerance. Both A. halleri and A. thaliana exert tight regulatory control over Zn homeostasis at the transcript level. Zn hyperaccumulation in A. halleri involves enhanced partitioning of Zn from roots into shoots. The transcriptional regulation of marker genes suggests that in the steady state, A. halleri roots, but not the shoots, act as physiologically Zn deficient under conditions of moderate Zn supply.

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FDR3 is another transporter belonging to the MATE (Multidrug and Toxin Efflux) gene family of transporters overexpressed in the root pericycle of A. halleri and T. caerulescens to hyperaccumulate Fe.
“FDR3 is another transporter belonging to the MATE (Multidrug and Toxin Efflux) gene family of transporters overexpressed in the root pericycle of A. halleri and T. caerulescens to hyperaccumulate Fe (Talke et al., 2006; van de Mortel et al., 2006).”
From Plant hyperaccumulators: a state-of-the-art review on mechanism of heavy metal transport and sequestration (2025) · cites this paper as Talke et al., 2006
The Discussion identifies FRD3 (not FDR3) as a multidrug and toxin efflux family protein and reports very high expression in A. halleri; the gene-expression results show elevated expression in roots. The paper does not establish expression specifically in the root pericycle, report FRD3 overexpression in T. caerulescens, or show that it causes Fe hyperaccumulation. It describes a suggested role in Fe mobility instead. (LLM, conf=0.88)
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Authors: Talke, I. N., Hanikenne, M., & Krämer, U.
Affiliations: Max Planck Institute of Molecular Plant Physiology (DE)
Max Planck Institute of Molecular Plant Physiology, D–14476 Potsdam-Golm, Germany
ORCID: Talke, Ina N.
Hanikenne, Marc
Venue: Plant Physiology
Publisher: Oxford University Press (OUP)
Published: 2006-07-14
Volume: 142
Issue: 1
Pages: 148-167
ISSN: 0032-0889 (ISSN-L)
1532-2548
DOI: 10.1104/pp.105.076232
PMC: PMC1557598
OpenAlex: W2124893845
Semantic Scholar: 31b109b115253c094652610d4e926f49dc2d3913
Cited by: 452
References: 82
Citations by year: 2026: 9 · 2025: 15 · 2024: 17 · 2023: 13 · 2022: 13 · 2021: 33 · 2020: 33 · 2019: 25 · 2018: 26 · 2017: 15 · 2016: 32 · 2015: 25 · 2014: 32 · 2013: 31 · 2012: 29
FWCI: 23.79
Type: journal article
Language: en
Open access: bronze
doi.org
Licence: academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model (vor)
Locations: PLANT PHYSIOLOGY (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Open Repository and Bibliography (University of Liège) (submittedVersion)
Max Planck Institute for Plasma Physics (submittedVersion)
MPG.PuRe (Max Planck Society) (submittedVersion)
Funders: European Commission
Deutsche Forschungsgemeinschaft
Bundesministerium für Bildung und Forschung
Topics: Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Micronutrient Interactions and Effects
Physical Sciences › Environmental Science › Pollution › Heavy metals in environment
Physical Sciences › Chemistry › Analytical Chemistry › Heavy Metals in Plants
Keywords: Arabidopsis halleri, zinc hyperaccumulation, cadmium tolerance, metal homeostasis, transcriptional regulation, gene copy number, HMA4, ZIP10, zinc influx, micronutrient homeostasis, Arabidopsis thaliana comparison, metal hyperaccumulators
MeSH terms: Adenosine Triphosphatases, Homeostasis, Transcription, Genetic, Zinc, Arabidopsis, Gene Expression Regulation, Plant, Gene Dosage, Reverse Transcriptase Polymerase Chain Reaction, Oligonucleotide Array Sequence Analysis, Gene Expression Profiling, Arabidopsis Proteins

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Zinc-Dependent Global Transcriptional Control, Transcriptional Deregulation, and Higher Gene Copy Number for Genes in Metal Homeostasis of the Hyperaccumulator Arabidopsis halleri

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