Cited by 203Plant Micronutrient Interactions and EffectsTrace Elements in HealthHeavy Metals in Plants

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MTP1-dependent Zn sequestration into shoot vacuoles suggests dual roles in Zn tolerance and accumulation in Zn-hyperaccumulating plants

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Gustin, Jeffery L., Loureiro, Marcello E., Kim, Donggiun, Na, Gunnam, Tikhonova, Marina, Salt, David E.·2009·The Plant Journal

DOI 10.1111/j.1365-313x.2008.03754.x

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Abstract

The integral membrane protein Thlaspi goesingense metal tolerance protein 1 (TgMTP1) has been suggested to play an important role in Zn hyperaccumulation in T. goesingense. Here, we show that the TgMTP1 protein is accumulated to high levels at the vacuolar membrane in shoot tissue of T. goesingense. TgMTP1 is likely to act in the transport of Zn into the vacuole, enhancing both Zn accumulation and tolerance. By specifically expressing TgMTP1 in Arabidopsis thaliana shoots, we show that TgMTP1, localized at the vacuolar membrane, can drive the enhanced shoot accumulation of Zn by initiating a systemic Zn deficiency response. The systematic response includes increased expression of Zn transporters (ZIP3, ZIP4, ZIP5 and ZIP9) in both shoot and root tissue. Furthermore, shoot-specific accumulation of TgMTP1 at the vacuolar membrane also leads to increased resistance to Zn in A. thaliana, probably through enhanced Zn compartmentalization in the vacuole. Such evidence leads to the conclusion that the high levels of TgMTP1 at the vacuolar membrane in shoot tissue of the Zn hyperaccumulator T. goesingense play a role in both Zn tolerance and enhanced Zn uptake and accumulation, via the activation of a systemic Zn deficiency response.

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“Our findings suggest that the enhanced Cd contents might be due to the up-regulation of various Zn transporter genes, such as AtZIP3 , AtZIP4 , and AtZIP9 , by CaWRKY41 (Supplementary Fig.”
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Background
Functional analysis of metal tolerance proteins isolated from Zn/Cd hyperaccumulating ecotype and non-hyperaccumulating ecotype of Sedum alfredii Hance
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Background
Physiology and Molecular Biology of Trace Element Hyperaccumulation
Merlot, S., de la Torre, V. S. G., & Hanikenne, M. (2017) · Mineral resource reviews · doi:10.1007/978-3-319-61899-9_6 · Cited by 44
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Background
Retracted
Genome-Wide Identification and Expression Analysis of the Cation Diffusion Facilitator Gene Family in Turnip Under Diverse Metal Ion Stresses
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“In this regard, free histidine has been proposed to play a key role in metal translocation through the xylem along with the participation of Zn transporters to take up the metal into the cytosol and vacuole (Mills et al. 2005; Gustin et al. 2009; Maestri et al. 2010).”
Background
Zinc-Solubilizing Bacteria: An Option to Increase Zinc Uptake by Plants
Haroon, M., Khan, S. T., & Malik, A. R. A. (2021) · Springer eBooks · doi:10.1007/978-3-030-76609-2_11 · Cited by 35

Authors: Gustin, J. L., Loureiro, M. E., Kim, D., Na, G., Tikhonova, M., & Salt, D. E.
Affiliations: Purdue University West Lafayette (US)
Universidade Federal de Viçosa (BR)
Gyeongsang National University (KR)
Corresponding author: Salt, David E.
ORCID: Gustin, Jeffery L.
Venue: The Plant Journal
Publisher: Wiley
Published: 2009-03
Volume: 57
Issue: 6
Pages: 1116-1127
ISSN: 0960-7412 (ISSN-L)
1365-313X
DOI: 10.1111/j.1365-313x.2008.03754.x
OpenAlex: W2119893617
Cited by: 203
References: 46
Citations by year: 2026: 7 · 2025: 7 · 2024: 7 · 2023: 8 · 2022: 4 · 2021: 24 · 2020: 18 · 2019: 15 · 2018: 8 · 2017: 10 · 2016: 12 · 2015: 8 · 2014: 13 · 2013: 14 · 2012: 11
FWCI: 14.92
Type: journal article
Language: en
Open access: bronze
doi.org
Licence: onlinelibrary.wiley.com/termsAndConditions#vor (vor)
Locations: The Plant Journal (publishedVersion)
PubMed (publishedVersion)
Funders: National Science Foundation
Purdue University
Topics: Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Micronutrient Interactions and Effects
Health Sciences › Nursing › Nutrition and Dietetics › Trace Elements in Health
Physical Sciences › Chemistry › Analytical Chemistry › Heavy Metals in Plants
Keywords: metal tolerance proteins, zinc hyperaccumulation, tonoplast, zinc tolerance, ZIP transporters, zinc uptake, Arabidopsis thaliana transformation, metal hyperaccumulator plants
MeSH terms: Amino Acid Sequence, Molecular Sequence Data, Plant Proteins, Vacuoles, Zinc, Sequence Alignment, Arabidopsis, Gene Expression Regulation, Plant, Plant Shoots, Cation Transport Proteins, Thlaspi

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MTP1-dependent Zn sequestration into shoot vacuoles suggests dual roles in Zn tolerance and accumulation in Zn-hyperaccumulating plants

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