The DOI matches its Crossref record; 45 of the 46 references it cites were found in a registry or an index.
MTP1-dependent Zn sequestration into shoot vacuoles suggests dual roles in Zn tolerance and accumulation in Zn-hyperaccumulating plants
Open accessAbstract
The integral membrane protein Thlaspi goesingense metal tolerance protein 1 (TgMTP1) has been suggested to play an important role in Zn hyperaccumulation in T. goesingense. Here, we show that the TgMTP1 protein is accumulated to high levels at the vacuolar membrane in shoot tissue of T. goesingense. TgMTP1 is likely to act in the transport of Zn into the vacuole, enhancing both Zn accumulation and tolerance. By specifically expressing TgMTP1 in Arabidopsis thaliana shoots, we show that TgMTP1, localized at the vacuolar membrane, can drive the enhanced shoot accumulation of Zn by initiating a systemic Zn deficiency response. The systematic response includes increased expression of Zn transporters (ZIP3, ZIP4, ZIP5 and ZIP9) in both shoot and root tissue. Furthermore, shoot-specific accumulation of TgMTP1 at the vacuolar membrane also leads to increased resistance to Zn in A. thaliana, probably through enhanced Zn compartmentalization in the vacuole. Such evidence leads to the conclusion that the high levels of TgMTP1 at the vacuolar membrane in shoot tissue of the Zn hyperaccumulator T. goesingense play a role in both Zn tolerance and enhanced Zn uptake and accumulation, via the activation of a systemic Zn deficiency response.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“Moreover, MTP1 was overexpressed in A. halleri and N. caerulescens to transport Zn and Cd (Dräger et al., 2004; Gustin et al., 2009).”
From Plant hyperaccumulators: a state-of-the-art review on mechanism of heavy metal transport and sequestration (2025) · cites this paper as Gustin et al., 2009
Experimental — accuracy not yet measuredThe cited source says what the claim attributes to it.
In the citing paper
“The transformation of TgMTP1 in A. thaliana resulted in enhanced accumulation of Zn into vacuoles, attributing to Zn tolerance (Gustin et al., 2009).”
From Plant hyperaccumulators: a state-of-the-art review on mechanism of heavy metal transport and sequestration (2025) · cites this paper as Gustin et al., 2009
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
[34] Chlorophylls and carotenoids: Pigments of photosynthetic biomembranes
Lichtenthaler, H. K. (1987) · Methods in Enzymology · doi:10.1016/0076-6879(87)48036-1 · Cited by 13,616
Salt, D. E., Smith, R. D., & Raskin, I. (1998) · Annual Review of Plant Physiology and Plant Molecular Biology · doi:10.1146/annurev.arplant.49.1.643 · Cited by 2,235
Self-citationAccumulators and excluders ‐strategies in the response of plants to heavy metals
Baker, A. J. M. (1981) · Journal of Plant Nutrition · doi:10.1080/01904168109362867 · Cited by 2,233
Mechanisms of Cadmium Mobility and Accumulation in Indian Mustard
Salt, D. E., Prince, R. C., Pickering, I. J., & Raskin, I. (1995) · PLANT PHYSIOLOGY · doi:10.1104/pp.109.4.1427 · Cited by 1,030
Self-citationEvolution of metal hyperaccumulation required cis-regulatory changes and triplication of HMA4
Hanikenne, M., Talke, I. N., Haydon, M. J., Lanz, C., Nolte, A., Motte, P., Kroymann, J., Weigel, D., & Krämer, U. (2008) · Nature · doi:10.1038/nature06877 · Cited by 812
Grotz, N., Fox, T. C., Connolly, E. L., Park, W. D., Guerinot, M. L., & Eide, D. J. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.12.7220 · Cited by 735
Küpper, H., Lombi, E., Zhao, F.-J., & McGrath, S. P. (2000) · Planta · doi:10.1007/s004250000366 · Cited by 685
Becher, M., Talke, I. N., Krall, L., & Krämer, U. (2004) · The Plant Journal · doi:10.1046/j.1365-313x.2003.01959.x · Cited by 568
van de Mortel, J. E., Almar Villanueva, L., Schat, H., Kwekkeboom, J., Coughlan, S., Moerland, P. D., Ver Loren van Themaat, E., Koornneef, M., & Aarts, M. G. M. (2006) · Plant Physiology · doi:10.1104/pp.106.082073 · Cited by 547
Talke, I. N., Hanikenne, M., & Krämer, U. (2006) · Plant Physiology · doi:10.1104/pp.105.076232 · Cited by 452
Genomic scale profiling of nutrient and trace elements in Arabidopsis thaliana
Lahner, B., Gong, J., Mahmoudian, M., Smith, E. L., Abid, K. B., Rogers, E. E., Guerinot, M. L., Harper, J. F., Ward, J. M., McIntyre, L. M., et al. (2003) · Nature Biotechnology · doi:10.1038/nbt865 · Cited by 446
Self-citationCellular Compartmentation of Zinc in Leaves of the Hyperaccumulator Thlaspi caerulescens1
Küpper, H., Zhao, F. J., & McGrath, S. P. (1999) · PLANT PHYSIOLOGY · doi:10.1104/pp.119.1.305 · Cited by 444
Weber, M., Harada, E., Vess, C., Roepenack‐Lahaye, E. V., & Clemens, S. (2004) · The Plant Journal · doi:10.1046/j.1365-313x.2003.01960.x · Cited by 440
Henri Wintz, A., Fox, T. C., Wu, Y., Feng, V., Chen, W., Chang, H., Zhu, T., & Vulpe, C. D. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m309338200 · Cited by 431
Arrivault, S., Senger, T., & Krämer, U. (2006) · The Plant Journal · doi:10.1111/j.1365-313x.2006.02746.x · Cited by 418
van der Zaal, B. J., Neuteboom, L. W., Pinas, J. E., Chardonnens, A. N., Schat, H., Verkleij, J. A. C., & Hooykaas, P. J. J. (1999) · PLANT PHYSIOLOGY · doi:10.1104/pp.119.3.1047 · Cited by 415
Boyes, D. C., Nam, J., & Dangl, J. L. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.26.15849 · Cited by 410
Molecular physiology of zinc transport in the Zn hyperaccumulator Thlaspi caerulescens
Lasat, M. M., Pence, N. S., Garvin, D. F., Ebbs, S. D., & Kochian, L. V. (2000) · Journal of Experimental Botany · doi:10.1093/jexbot/51.342.71 · Cited by 344
Selective and powerful stress gene expression in Arabidopsis in response to malondialdehyde
Weber, H. H., Chételat, A., Reymond, P., & Farmer, E. E. (2004) · The Plant Journal · doi:10.1111/j.1365-313x.2003.02013.x · Cited by 326
Persans, M. W., Nieman, K., & Salt, D. E. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.171039798 · Cited by 317
Self-citationKobae, Y., Uemura, T., Sato, M. H., Ohnishi, M., Mimura, T., Nakagawa, T., & Maeshima, M. (2004) · Plant and Cell Physiology · doi:10.1093/pcp/pci015 · Cited by 311
Desbrosses-Fonrouge, A.-G., Voigt, K., Schröder, A., Arrivault, S., Thomine, S., & Krämer, U. (2005) · FEBS Letters · doi:10.1016/j.febslet.2005.06.046 · Cited by 305
Natural Variants of AtHKT1 Enhance Na+ Accumulation in Two Wild Populations of Arabidopsis
Rus, A. M., Baxter, I. R., Balasubramaniam, M., Gustin, J., Lahner, B., Yakubova, E., & Salt, D. E. (2006) · PLoS Genetics · doi:10.1371/journal.pgen.0020210 · Cited by 299
Self-citationKüpper, H., Mijovilovich, A., Meyer‐Klaucke, W., & Kroneck, P. M. H. (2004) · PLANT PHYSIOLOGY · doi:10.1104/pp.103.032953 · Cited by 298
Dräger, D. B., Desbrosses‐Fonrouge, A., Krach, C., Chardonnens, A. N., Meyer, R. C., Saumitou‐Laprade, P., & Krämer, U. (2004) · The Plant Journal · doi:10.1111/j.1365-313x.2004.02143.x · Cited by 294
European species of Thlaspi L.(Cruciferae) as indicators of nickel and zinc
Reeves, R. D., & Brooks, R. R. (1983) · Journal of Geochemical Exploration · doi:10.1016/0375-6742(83)90073-0 · Cited by 207
Medina‐Escobar, N., Haupt, S., Thow, G., Boevink, P. C., Chapman, S. N., & OPARKA, K. J. (2003) · The Plant Cell · doi:10.1105/tpc.013284 · Cited by 204
Blaudez, D., Kohler, A., Martin, F., Sanders, D., & Chalot, M. (2003) · The Plant Cell · doi:10.1105/tpc.017541 · Cited by 200
Saito, C., Ueda, T., Abe, H., Wada, Y., Kuroiwa, T., Hisada, A., Furuya, M., & Nakano, A. (2002) · The Plant Journal · doi:10.1046/j.0960-7412.2001.01189.x · Cited by 198
Zinc tolerance and hyperaccumulation are genetically independent characters
Macnair, M. R., Bert, V., Huitson, S. B., Saumitou‐Laprade, P., & Petit, D. (1999) · Proceedings of the Royal Society B Biological Sciences · doi:10.1098/rspb.1999.0905 · Cited by 196
Willems, G., Dräger, D. B., Courbot, M., Godé, C., Verbruggen, N., & Saumitou-Laprade, P. (2007) · Genetics · doi:10.1534/genetics.106.064485 · Cited by 182
Structure of a Plasma Membrane H+-ATPase Gene from the Plant Arabidopsis thaliana
Pardo, J. M., & Serrano, R. (1989) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)81827-0 · Cited by 171
Kim, D., Gustin, J. L., Lahner, B., Persans, M. W., Baek, D., Yun, D., & Salt, D. E. (2004) · The Plant Journal · doi:10.1111/j.1365-313x.2004.02126.x · Cited by 148
Self-citationPeer, W. A., Mamoudian, M., Lahner, B., Reeves, R. D., Murphy, A. S., & Salt, D. E. (2003) · New Phytologist · doi:10.1046/j.1469-8137.2003.00822.x · Cited by 131
Self-citationDeniau, A. X., Pieper, B., Bookum, W. M. T., Lindhout, P., Aarts, M. G. M., & Schat, H. (2006) · Theoretical and Applied Genetics · doi:10.1007/s00122-006-0350-y · Cited by 120
Vacuolar membrane dynamics revealed by GFP‐AtVam3 fusion protein
Uemura, T., Yoshimura, S. H., Takeyasu, K., & Sato, M. H. (2002) · Genes to Cells · doi:10.1046/j.1365-2443.2002.00550.x · Cited by 110
Dissociation and Reassembly of the Vacuolar H+-ATPase Complex from Oat Roots
Ward, J. M., Reinders, A., Hsu, H. T., & Sze, H. (1992) · PLANT PHYSIOLOGY · doi:10.1104/pp.99.1.161 · Cited by 89
Responses of plants to iron, zinc and copper deficiencies
Wintz, H., Fox, T. C., & Vulpe, C. D. (2002) · Biochemical Society Transactions · doi:10.1042/bst0300766 · Cited by 84
Assunção, A. G. L., Pieper, B., Vromans, J., Lindhout, P., Aarts, M. G. M., & Schat, H. (2005) · New Phytologist · doi:10.1111/j.1469-8137.2005.01631.x · Cited by 84
Assunção, A. G. L., Bookum, W. M. T., Nelissen, H. J. M., Vooijs, R., Schat, H., & Ernst, W. H. O. (2003) · New Phytologist · doi:10.1046/j.1469-8137.2003.00758.x · Cited by 80
Pauwels, M., WILLEMS, G., Roosens, N. H. C., Frérot, H., & Saumitou‐Laprade, P. (2007) · Molecular Ecology · doi:10.1111/j.1365-294x.2007.03486.x · Cited by 51
A quantitative trait loci analysis of Zinc hyperaccumulation in Arabidopsis halleri
Filatov, V., Dowdle, J., Smirnoff, N., Ford-Lloyd, B. V., Newbury, H. J., & Macnair, M. R. (2007) · New Phytologist · doi:10.1111/j.1469-8137.2007.02036.x · Cited by 42
Frérot, H., Petit, C., Lefèbvre, C., Grüber, W., Collin, C., & Escarré, J. (2003) · New Phytologist · doi:10.1046/j.1469-8137.2003.00701.x · Cited by 42
Isolation of Membranes and Organelles from Plant Cells
Rao, K. K. (1984) · FEBS Letters · doi:10.1016/0014-5793(84)81407-6 · Cited by 41
Frérot, H., Lefèbvre, C., Petit, C., Collin, C., dos Santos, A., & Escarré, J. (2004) · New Phytologist · doi:10.1111/j.1469-8137.2004.01227.x · Cited by 38
Research works printed but not linked: 3
- 10.1371/journal.pgen.00202102 · DOI
- Sambrook J., 2001, Molecular Cloning, A Laboratory Manual
- Weigel D., 2002, Arabidosis A Laboratory Manual
Cited by
Sorted by citations
Heavy metal hyperaccumulating plants: how and why do they do it? And what makes them so interesting
Rascio, N., & Navari-Izzo, F. (2011) · Plant Science · doi:10.1016/j.plantsci.2010.08.016 · Cited by 2,012
“sion of ZIP transporters in hyperaccumulator plants [105].”
BackgroundMetal hyperaccumulation in plants
Krämer, U. (2010) · Annual Review of Plant Biology · doi:10.1146/annurev-arplant-042809-112156 · Cited by 1,284
The molecular mechanism of zinc and cadmium stress response in plants
Lin, Y., & Aarts, M. G. M. (2012) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-012-1089-z · Cited by 689
Facing the challenges of Cu, Fe and Zn homeostasis in plants
Palmer, C. M., & Guerinot, M. L. (2009) · Nature Chemical Biology · doi:10.1038/nchembio.166 · Cited by 633
Approaches for enhanced phytoextraction of heavy metals
Bhargava, A., Carmona, F. F. F., Bhargava, M., & Srivastava, S. (2012) · Journal of Environmental Management · doi:10.1016/j.jenvman.2012.04.002 · Cited by 585
Metadata
- Authors
- Gustin, J. L., Loureiro, M. E., Kim, D., Na, G., Tikhonova, M., & Salt, D. E.
- Affiliations
- Purdue University West Lafayette (US)
- Universidade Federal de Viçosa (BR)
- Gyeongsang National University (KR)
- Corresponding author
- Salt, David E.
- ORCID
- Gustin, Jeffery L.
- Venue
- The Plant Journal
- Publisher
- Wiley
- Published
- 2009-03
- Volume
- 57
- Issue
- 6
- Pages
- 1116-1127
- ISSN
- 0960-7412 (ISSN-L)
- 1365-313X
- OpenAlex
- W2119893617
- Cited by
- 203
- References
- 46
- Citations by year
- 2026: 7 · 2025: 7 · 2024: 7 · 2023: 8 · 2022: 4 · 2021: 24 · 2020: 18 · 2019: 15 · 2018: 8 · 2017: 10 · 2016: 12 · 2015: 8 · 2014: 13 · 2013: 14 · 2012: 11
- FWCI
- 14.92
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- The Plant Journal (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Science Foundation
- Purdue University
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Micronutrient Interactions and Effects
- Health Sciences › Nursing › Nutrition and Dietetics › Trace Elements in Health
- Physical Sciences › Chemistry › Analytical Chemistry › Heavy Metals in Plants
- Keywords
- metal tolerance proteins, zinc hyperaccumulation, tonoplast, zinc tolerance, ZIP transporters, zinc uptake, Arabidopsis thaliana transformation, metal hyperaccumulator plants
- MeSH terms
- Amino Acid Sequence, Molecular Sequence Data, Plant Proteins, Vacuoles, Zinc, Sequence Alignment, Arabidopsis, Gene Expression Regulation, Plant, Plant Shoots, Cation Transport Proteins, Thlaspi
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
