Genetic deletion and pharmacological blockade of CB1 receptors modulates anxiety in the shock‐probe burying test
Abstract
Cannabinoids affect various behavioral processes, including emotion, learning and memory, which may bspecifically regulated through the CB1 receptors. The exact role CB1 receptors play in anxiety remains unclear. Both genetic and pharmacological blockade of CB1 receptors have produced inconsistent effects on anxiety. However, these studies examined passive avoidance as an index of anxiety. In the present study, both active and passive avoidance were examined using the shock‐probe burying test while CB1 receptors were blocked genetically or pharmacologically. In the shock‐probe burying test, anxiety is reflected by increased burying (increased active avoidance) and increased freezing (increased passive avoidance). In addition, probe‐contacts may reflect cognitive performance and/or passive avoidance. As there have been few studies examining mouse behavior in the shock‐probe burying test, experiment 1 was designed to pharmacologically validate this model in mice. Our results indicated that administration (i.p.) of chlordiazepoxide (4 mg/kg) or FG7412 (5 mg/kg) decreased and increased burying behavior, respectively, without affecting freezing or the number of probe contacts. Experiments 2 and 3 showed that both CB1 knockout mice and mice injected (i.p.) with 3 or 10 mg/kg, but not 1 mg/kg, of the CB1 receptor antagonist SR141716A had lower burying scores, fewer contacts with the probe and similar freezing times compared with wild‐type mice and mice injected with vehicle (experiments 2 and 3). Collectively, these results suggest that CB1 receptor blockade reduces some, but not all, aspects of anxiety. The decrease in probe contacts induced by CB1 receptor blockade may be due to enhanced cognition.
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Rodents will bury an electrified probe after having received shocks from the probe and will spontaneously bury marbles introduced as a novelty into their home cages.
“Rodents will bury an electrified probe after having received shocks from the probe43 and will even spontaneously bury marbles introduced as a novelty into their home cages44.”
From Resolving the neural circuits of anxiety (2015) · cites this paper as [43]
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- Authors
- Degroot, A., & Nomikos, G. G.
- Affiliations
- Eli Lilly (United States) (US)
- ORCID
- Nomikos, George G.
- Venue
- European Journal of Neuroscience
- Publisher
- Wiley
- Published
- 2004-08
- Volume
- 20
- Issue
- 4
- Pages
- 1059-1064
- ISSN
- 0953-816X (ISSN-L)
- 1460-9568
- OpenAlex
- W1966096012
- Cited by
- 58
- References
- 53
- Citations by year
- 2026: 1 · 2025: 4 · 2023: 3 · 2021: 1 · 2020: 3 · 2018: 1 · 2017: 1 · 2016: 1 · 2015: 8 · 2013: 2 · 2012: 4
- FWCI
- 3.98
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- European Journal of Neuroscience (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Pharmacology › Cannabis and Cannabinoid Research
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurotransmitter Receptor Influence on Behavior
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Keywords
- CB1 receptor, cannabinoid receptors, anxiety, active avoidance, passive avoidance, freezing behavior, CB1 knockout mice, SR141716A, chlordiazepoxide, cognitive performance
- MeSH terms
- Rimonabant, Animals, Anxiety, Carbolines, Chlordiazepoxide, Electroshock, Male, Mice, Inbred C57BL, Piperidines, Pyrazoles, Gene Deletion, Mice, Knockout, Receptor, Cannabinoid, CB1, Mice
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