Ultrastructural organization of medial prefrontal inputs to the rhinal cortices
Abstract
Accumulating evidence suggests that the medial prefrontal cortex (mPFC) plays a critical role in the formation, retrieval and long-term storage of hippocampal-dependent memories. Consistent with this, there are direct hippocampal projections to the mPFC. Moreover, the mPFC sends robust projections to the perirhinal and entorhinal cortices, two interconnected cortical fields that funnel information into and out of the hippocampus. However, the significance of the latter projection remains unclear because no data are available regarding the rhinal targets of mPFC axons. This question was examined in the present study using a combination of anterograde tracing with Phaseolus vulgaris leucoagglutinin and pre-embedding gamma-aminobutyric acid (GABA) immunocytochemistry in guinea pigs. Following Phaseolus vulgaris leucoagglutinin injections in the mPFC, anterogradely labeled axons were seen in the perirhinal (mainly superficial layers) and lateral entorhinal (mainly deep layers) cortices. In the electron microscope, the synaptic articulation of anterogradely labeled mPFC axon terminals with perirhinal and entorhinal neurons was found to be nearly identical. In these two rhinal fields, mPFC axon terminals only formed asymmetric synapses, typically with GABA-immunonegative spines ( approximately 70%) but occasionally with dendritic profiles ( approximately 30%), half of which were GABA immunopositive. In the light of earlier observations, these findings indicate that mPFC inputs exert mainly excitatory effects in the rhinal cortices, prevalently on principal neurons. Thus, these results suggest that the mPFC may affect hippocampal-dependent memories by enhancing impulse traffic into and out of the hippocampus at the level of the rhinal cortices.
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The nucleus reuniens (NR) in the medial thalamus, the perirhinal cortex and LEC are mutually connected with the PFC and hippocampus.
“The nucleus reuniens (NR) in the medial thalamus, the perirhinal cortex and LEC are mutually connected with the PFC and hippocampus”
From The medial prefrontal cortex - hippocampus circuit that integrates information of object, place and time to construct episodic memory in rodents: Behavioral, anatomical and neurochemical properties (2020) · cites this paper as Apergis-Schoute et al., 2006
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- Authors
- Apergis‐Schoute, J., Pinto, A., & Paré, D.
- Affiliations
- Rutgers, The State University of New Jersey (US)
- Corresponding author
- Paré, Denis
- Venue
- European Journal of Neuroscience
- Publisher
- Wiley
- Published
- 2006-07
- Volume
- 24
- Issue
- 1
- Pages
- 135-144
- ISSN
- 0953-816X (ISSN-L)
- 1460-9568
- OpenAlex
- W2035523884
- Cited by
- 51
- References
- 66
- Citations by year
- 2025: 2 · 2024: 1 · 2022: 4 · 2021: 4 · 2020: 6 · 2019: 1 · 2018: 1 · 2017: 4 · 2015: 4 · 2014: 1 · 2013: 6 · 2012: 3
- FWCI
- 1.13
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- European Journal of Neuroscience (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health
- U.S. Public Health Service (073610)
- National Institute of Mental Health
- Topics
- Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Cognitive Neuroscience › Memory Processes and Influences
- Keywords
- medial prefrontal cortex, perirhinal cortex, entorhinal cortex, hippocampal-dependent memory, hippocampal projections, synaptic ultrastructure, asymmetric synapses, GABAergic synapses, axon terminals, principal neurons, guinea pig
- MeSH terms
- Animals, Axons, gamma-Aminobutyric Acid, Guinea Pigs, Immunohistochemistry, Male, Microscopy, Electron, Phytohemagglutinins, Synapses, Prefrontal Cortex, Entorhinal Cortex, Parahippocampal Gyrus, Phaseolus, Dendritic Spines
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