The DOI matches its Crossref record; 51 of the 51 references it cites were found in a registry or an index.
Vesicular transport in Histoplasma capsulatum: an effective mechanism for trans-cell wall transfer of proteins and lipids in ascomycetes
Open accessAbstract
Vesicular secretion of macromolecules has recently been described in the basidiomycete Cryptococcus neoformans, raising the question as to whether ascomycetes similarly utilize vesicles for transport. In the present study, we examine whether the clinically important ascomycete Histoplasma capsulatum produce vesicles and utilized these structures to secrete macromolecules. Transmission electron microscopy (TEM) shows transcellular secretion of vesicles by yeast cells. Proteomic and lipidomic analyses of vesicles isolated from culture supernatants reveal a rich collection of macromolecules involved in diverse processes, including metabolism, cell recycling, signalling and virulence. The results demonstrate that H. capsulatum can utilize a trans-cell wall vesicular transport secretory mechanism to promote virulence. Additionally, TEM of supernatants collected from Candida albicans, Candida parapsilosis, Sporothrix schenckii and Saccharomyces cerevisiae documents that vesicles are similarly produced by additional ascomycetes. The vesicles from H. capsulatum react with immune serum from patients with histoplasmosis, providing an association of the vesicular products with pathogenesis. The findings support the proposal that vesicular secretion is a general mechanism in fungi for the transport of macromolecules related to virulence and that this process could be a target for novel therapeutics.
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1 retracted
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Cited by
Deatherage, B. L., & Cookson, B. T. (2012) · Infection and Immunity · doi:10.1128/iai.06014-11 · Cited by 770
Fungal Melanin: What do We Know About Structure?
Nosanchuk, J. D., Stark, R. E., & Casadevall, A. (2015) · Frontiers in Microbiology · doi:10.3389/fmicb.2015.01463 · Cited by 310
Self-citationCandida albicans biofilm–induced vesicles confer drug resistance through matrix biogenesis
Zarnowski, R., Sanchez, H., Covelli, A. S., Dominguez, E., Jaromin, A., Bernhardt, J., Mitchell, K. F., Heiss, C., Azadi, P., Mitchell, A., et al. (2018) · PLOS Biology · doi:10.1371/journal.pbio.2006872 · Cited by 260
Extracellular Vesicles from Cryptococcus neoformans Modulate Macrophage Functions
Oliveira, D. L., Freire-de-Lima, C. G., Nosanchuk, J. D., Casadevall, A., Rodrigues, M. L., & Nimrichter, L. (2010) · Infection and Immunity · doi:10.1128/iai.01171-09 · Cited by 257
Self-citationInteraction of pathogenic yeasts with phagocytes: survival, persistence and escape.
Seider, K., Heyken, A., Lüttich, A., Miramón, P., & Hube, B. (2010) · Current Opinion in Microbiology · doi:10.1016/j.mib.2010.05.001 · Cited by 167
Metadata
- Authors
- Albuquerque, P. C., Nakayasu, E. S., Rodrigues, M. L., Frases, S., Casadevall, A., Zancope-Oliveira, R. M., Almeida, I. C., & Nosanchuk, J. D.
- Venue
- Cellular Microbiology
- Publisher
- Hindawi Limited
- Published
- 2008-08
- Volume
- 10
- Issue
- 8
- Pages
- 1695-1710
- ISSN
- 1462-5814 (ISSN-L)
- 1462-5822
- OpenAlex
- W2105706954
- Cited by
- 351
- References
- 51
- FWCI
- 10.51
- Type
- journal article
- Language
- en
- Open access
- bronze
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- CoChat. (2026). Citation check: Vesicular transport in Histoplasma capsulatum: an effective mechanism for trans-cell wall transfer of proteins and lipids in ascomycetes. https://app.cochat.ai/papers/10.1111/j.1462-5822.2008.01160.x
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- Albuquerque, P. C., Nakayasu, E. S., Rodrigues, M. L., Frases, S., Casadevall, A., Zancope-Oliveira, R. M., Almeida, I. C., & Nosanchuk, J. D. (2008). Vesicular transport in Histoplasma capsulatum: an effective mechanism for trans-cell wall transfer of proteins and lipids in ascomycetes. Cellular Microbiology. https://doi.org/10.1111/j.1462-5822.2008.01160.x
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