The DOI matches its Crossref record; 32 of the 32 references it cites were found in a registry or an index.
Voltage‐gated and resting membrane currents recorded from B‐cells in intact mouse pancreatic islets
Open accessAbstract
1. The perforated patch whole-cell configuration of the patch-clamp technique was applied to superficial cells in intact pancreatic islets. Immunostaining in combination with confocal microscopy revealed that the superficial cells consisted of 35 % insulin-secreting B-cells and 65 % non-B-cells (A- and D-cells). 2. Two types of cell, with distinct electrophysiological properties, could be functionally identified. One of these generated oscillatory electrical activity when the islet was exposed to 10 mM glucose and had the electrophysiological characteristics of isolated B-cells maintained in tissue culture. 3. The Ca2+ current recorded from B-cells in situ was 80 % larger than that of isolated B-cells. It exhibited significant (70 %) inactivation during 100 ms depolarisations. The inactivation was voltage dependent and particularly prominent during depolarisations evoking the largest Ca2+ currents. 4. Voltage-dependent K+ currents were observed during depolarisations to membrane potentials above -20 mV. These currents inactivated little during a 200 ms depolarisation and were unaffected by varying the holding potential between -90 and -30 mV. 5. The maximum resting conductance in the absence of glucose, which reflects the conductance of ATP-regulated K+ (KATP) channels, amounted to approximately 4 nS. Glucose produced a concentration-dependent reduction of KATP channel conductance with half-maximal inhibition observed with 5 mM glucose. 6. Combining voltage- and current-clamp…
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In the citing paper
“The reported (244) current-voltage relationship of the glucose-activated CFTR current in metabolically intact mouse β-cells corresponds to a whole-cell conductance of 20 nS. This is 5-fold larger than the resting conductance recorded from metabolically intact β-cells in the absence of glucose (221), and 10-fold larger than the peak voltage-gated Ca2+ current (see legend to Figure 9) (221). With CF”
From Pancreatic β-Cell Electrical Activity and Insulin Secretion: Of Mice and Men (2018) · cites this paper as [221]
In the citing paper
“Steady-state inactivation is half-maximal at -40 mV, ≈60 mV more positive than the corresponding value in mouse β-cells (221, 514) (Figure 10).”
From Pancreatic β-Cell Electrical Activity and Insulin Secretion: Of Mice and Men (2018) · cites this paper as [221]
In the citing paper
“The mouse (and rat) β-cell Na+ channel is highly unusual in the voltage dependence of its inactivation. In most β-cells, it inactivates at such negative membrane potentials that little or no Na+ current remains within the physiological range of membrane potentials (221, 514, 761) (Figure 10).”
From Pancreatic β-Cell Electrical Activity and Insulin Secretion: Of Mice and Men (2018) · cites this paper as [221]
In the citing paper
“The membrane conductance of β-cells in intact islets (measured in perforated patch recordings at 20 mM glucose as outlined in Figure 11A) is ~1 nS (221), 10-fold higher than that observed in dispersed single β-cells (631).”
From Pancreatic β-Cell Electrical Activity and Insulin Secretion: Of Mice and Men (2018) · cites this paper as [221]
In the citing paper
“The background inward current must depolarize the membrane to ~-50 mV, which is the threshold for electrical activity and the membrane potential at which voltage-gated Ca2+ channels start to open (221) (Figure 9).”
From Pancreatic β-Cell Electrical Activity and Insulin Secretion: Of Mice and Men (2018) · cites this paper as [221]
In the citing paper
“A β-cell with a diameter of 15 µm will therefore have a capacitance of ~7 pF, close to that measured experimentally (221).”
From Pancreatic β-Cell Electrical Activity and Insulin Secretion: Of Mice and Men (2018) · cites this paper as [221]
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Pancreatic β-Cell Electrical Activity and Insulin Secretion: Of Mice and Men
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Self-citationRegulation of Insulin Secretion in Human Pancreatic Islets
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Self-citation
Metadata
- Authors
- Göpel, S., Kanno, T., Barg, S., Galvanovskis, J., & Rorsman, P.
- Affiliations
- Lund University (SE)
- ORCID
- Venue
- The Journal of Physiology
- Publisher
- Wiley
- Published
- 1999-12
- Volume
- 521
- Issue
- 3
- Pages
- 717-728
- ISSN
- 0022-3751 (ISSN-L)
- 1469-7793
- PMC
- PMC2269694
- OpenAlex
- W2017509811
- Cited by
- 226
- References
- 32
- Citations by year
- 2026: 4 · 2025: 2 · 2024: 5 · 2023: 4 · 2022: 7 · 2021: 10 · 2020: 7 · 2019: 7 · 2018: 4 · 2017: 10 · 2016: 8 · 2015: 9 · 2014: 8 · 2013: 12 · 2012: 12
- FWCI
- 5.27
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- The Journal of Physiology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Lund University Publications (Lund University) (submittedVersion)
- Topics
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Pancreatic function and diabetes
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Connexins and lens biology
- Keywords
- pancreatic islets, B cells, voltage-gated calcium currents, voltage-dependent potassium current, ATP-sensitive potassium channels, electrical coupling, glucose stimulation, insulin secretion
- MeSH terms
- Algorithms, Animals, Cell Communication, Electrophysiology, Glucose, Islets of Langerhans, Membrane Potentials, Tetrodotoxin, Calcium Channels, Potassium Channels, Sodium Channels, Ion Channel Gating, Gap Junctions, Patch-Clamp Techniques, ATP-Binding Cassette Transporters, Microscopy, Confocal, Potassium Channels, Inwardly Rectifying, Mice, KATP Channels
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