Effect of administration of oral contraceptives on the synthesis and breakdown of myofibrillar proteins in young women
Abstract
Oral contraceptive (OC) treatment has an inhibiting effect on protein synthesis in tendon and muscle connective tissue. We aimed to investigate whether OC influence myofibrillar protein turnover in young women. OC‐users (24±2 years; Lindynette®n=7, Cilest®n=4) and non‐OC‐users (controls, 24±4 years n=12) performed one‐legged kicking exercise. The next day, the myofibrillar protein fractional synthesis rate (FSR) was measured using stable isotopic tracers (13 C‐proline) while the subjects were fed standardized nutrient drinks. Simultaneously, a marker for myofibrillar protein breakdown, 3‐methyl‐histidine (3‐MH), was measured in the interstitial fluid of the vastus lateralis. Measurements were performed in both legs. In general, myofibrillar protein FSR was lower in OC‐users (two‐way analysis of variance, P<0.05), although the difference seemed to depend on the OC type. Interstitial 3‐MH in the skeletal muscle was not different between groups and did not vary by OC type. Exercise did not change myofibrillar protein FSR or 3‐MH concentrations. Serum androstenedione and bioavailability of testosterone were lower in OC‐users. In conclusion, the results indicate that the use of OC has an inhibiting effect on myofibrillar protein synthesis and the magnitude of the effect may depend on the type of OC. In contrast, there was no effect of OC on myofibrillar protein breakdown in the fed state.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Blood amino acid profiles of COC users differ from non-users.
“Although the blood amino acid profiles of COC users are shown to differ from non-users [225]”
From International society of sports nutrition position stand: nutritional concerns of the female athlete (2023) · cites this paper as [225]
Cites
1 retracted
Application of cellular mechanisms to growth and development of food producing animals1,2
Chung, K. Y., & Johnson, B. J. (2007) · Journal of Animal Science · doi:10.2527/jas.2007-0450 · Cited by 21 · see citing works
RetractedA Critical Evaluation of Simple Methods for the Estimation of Free Testosterone in Serum
Vermeulen, A., Verdonck, L., & Kaufman, J. M. (1999) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem.84.10.6079 · Cited by 3,824 · see citing works
Anterior Cruciate Ligament Injuries in Female Athletes
Hewett, T. E., Ford, K. R., & Myer, G. D. (2005) · The American Journal of Sports Medicine · doi:10.1177/0363546505282619 · Cited by 714 · see citing works
Miller, B. F., Olesen, J. L., Hansen, M., Døssing, S., Crameri, R. M., Welling, R. J., Langberg, H., Flyvbjerg, A., Kjaer, M., Babraj, J. A., et al. (2005) · The Journal of Physiology · doi:10.1113/jphysiol.2005.093690 · Cited by 610 · see citing works
Self-citationPhillips, S. K., Rook, K. M., SIDDLE, N. C., Bruce, S., & WOLEDGE, R. C. (1993) · Clinical Science · doi:10.1042/cs0840095 · Cited by 506 · see citing works
Age and aerobic exercise training effects on whole body and muscle protein metabolism
Short, K. R., Vittone, J. L., Bigelow, M. L., Proctor, D. N., & Nair, K. S. (2004) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00366.2003 · Cited by 470 · cited 1× · see citing works
“There has been a lack of documented gender difference in wholebody protein turnover and muscle protein kinetics after adjustment of differences in muscle mass, except for a higher leucine oxidation in men during exercise (Volpi et al., 1998; Short et al., 2004; Miller et al., 2006).”
BackgroundApplied gas chromatography coupled to isotope ratio mass spectrometry
Meier‐Augenstein, W. (1999) · Journal of Chromatography A · doi:10.1016/s0021-9673(98)01057-7 · Cited by 426 · see citing works
Langberg, H., Skovgaard, D. C., Petersen, L. J., Bülow, J., & Kjær, M. (1999) · The Journal of Physiology · doi:10.1111/j.1469-7793.1999.00299.x · Cited by 350 · see citing works
Self-citationResistance training reduces the acute exercise-induced increase in muscle protein turnover
Phillips, S. M., Tipton, K. D., Ferrando, A. A., & Wolfe, R. R. (1999) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.1999.276.1.e118 · Cited by 336 · see citing works
P ROTEIN AND A MINO A CID M ETABOLISM D URING AND A FTER E XERCISE AND THE E FFECTS OF N UTRITION
Rennie, M. J., & Tipton, K. D. (2000) · Annual Review of Nutrition · doi:10.1146/annurev.nutr.20.1.457 · Cited by 321 · see citing works
Proof of the effect of testosterone on skeletal muscle
Bhasin, S., Woodhouse, L. J., & Storer, T. W. (2001) · Journal of Endocrinology · doi:10.1677/joe.0.1700027 · Cited by 294 · see citing works
Schwenk, W. F., Berg, P. J., Beaufrère, B., Miles, J. M., & Haymond, M. W. (1984) · Analytical Biochemistry · doi:10.1016/0003-2697(84)90431-7 · Cited by 231 · see citing works
Effects of flooding amino acids on incorporation of labeled amino acids into human muscle protein
Smith, K., Reynolds, N., Downie, S., Patel, A. A., & Rennie, M. J. (1998) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.1998.275.1.e73 · Cited by 197 · see citing works
Collagen synthesis in human musculoskeletal tissues and skin
Babraj, J., Cuthbertson, D. J., Smith, K., Langberg, H., Miller, B. F., Krogsgaard, M. R., Kjær, M., & Rennie, M. J. (2005) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00243.2005 · Cited by 177 · see citing works
Self-citationResistance-training-induced adaptations in skeletal muscle protein turnover in the fed state
Phillips, S. M., Parise, G., Roy, B. D., Tipton, K. D., Wolfe, R. R., & Tarnopolsky, M. A. (2002) · Canadian Journal of Physiology and Pharmacology · doi:10.1139/y02-134 · Cited by 164 · see citing works
Estrogen status and skeletal muscle recovery from disuse atrophy
McClung, J. M., Davis, J. M., Wilson, M. A., Goldsmith, E. C., & Carson, J. A. (2006) · Journal of Applied Physiology · doi:10.1152/japplphysiol.01583.2005 · Cited by 147 · see citing works
Hansen, M., Miller, B. F., Holm, L., Doessing, S., Petersen, S. G., Skovgaard, D. C., Frystyk, J., Flyvbjerg, A., Koskinen, S., Pingel, J., et al. (2008) · Journal of Applied Physiology · doi:10.1152/japplphysiol.90933.2008 · Cited by 146 · see citing works
Self-citationHamadeh, M. J., Devries, M. C., & Tarnopolsky, M. A. (2005) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2004-1743 · Cited by 144 · see citing works
Toth, M. J., Poehlman, É. T., Matthews, D. E., Tchernof, A., & MacCoss, M. J. (2001) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.2001.280.3.e496 · Cited by 134 · see citing works
Wiik, A., Glenmark, B., Ekman, M., Esbjörnsson‐Liljedahl, M., Johansson, O., Bodin, K., Enmark, E., & Jansson, E. (2003) · Acta Physiologica Scandinavica · doi:10.1046/j.0001-6772.2003.01186.x · Cited by 122 · see citing works
Miller, B. F., Hansen, M., Olesen, J. L., Flyvbjerg, A., Schwarz, P., Babraj, J. A., Smith, K., Rennie, M. J., & Kjaer, M. (2006) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00300.2005 · Cited by 111 · see citing works
Self-citationWelle, S. L., & Thornton, C. A. (1998) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.1998.274.4.e677 · Cited by 102 · see citing works
Resistance Training in Postmenopausal Women with and without Hormone Therapy
Teixeira, P. J., Going, S. B., HOUTKOOPER, L. B., METCALFE, L. L., Blew, R. M., FLINT-WAGNER, H. G., CUSSLER, E. C., Sardinha, L. B., & Lohman, T. G. (2003) · Medicine & Science in Sports & Exercise · doi:10.1249/01.mss.0000058437.17262.11 · Cited by 92 · see citing works
Comparison of Injury During Cadet Basic Training by Gender
Bijur, P. E. (1997) · Archives of Pediatrics and Adolescent Medicine · doi:10.1001/archpedi.1997.02170420026004 · Cited by 89 · see citing works
Hansen, M., Koskinen, S., Petersen, S. G., Doessing, S., Frystyk, J., Flyvbjerg, A., Westh, E. K., Magnusson, S. P., Kjær, M., & Langberg, H. (2008) · The Journal of Physiology · doi:10.1113/jphysiol.2007.147348 · Cited by 89 · see citing works
Self-citationRelationship between endogenous 3-methylhistidine excretion and body composition
Lukaski, H. C., Méndez, J., BUSKIRK, E. R., & Cohn, S. H. (1981) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.1981.240.3.e302 · Cited by 88 · see citing works
Morin-Papunen, L., Martikainen, H., McCarthy, M. I., Franks, S., Sovio, U., Hartikainen, A.-L., Ruokonen, A., Leinonen, M., Laitinen, J., Järvelin, M.-R., et al. (2008) · American Journal of Obstetrics and Gynecology · doi:10.1016/j.ajog.2008.04.013 · Cited by 78 · see citing works
Reubinoff, B. E., Grubstein, A., Meirow, D., et al. (1995) · Fertility and Sterility · doi:10.1016/s0015-0282(16)57419-6 · Cited by 68 · see citing works
Progesterone stimulates cardiac muscle protein synthesis via receptor-dependent pathway
Goldstein, J. S., Sites, C. K., & Toth, M. J. (2004) · Fertility and Sterility · doi:10.1016/j.fertnstert.2004.03.018 · Cited by 58 · see citing works
Gender Differences in Basal Protein Kinetics in Young Adults
Volpi, E., Lucidi, P., Bolli, G. B., Santeusanio, F., & Feo, P. D. (1998) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem.83.12.5330 · Cited by 53 · see citing works
Sequential extracts of human bone show differing collagen synthetic rates
Babraj, J., Cuthbertson, D. J., Rickhuss, P. K., Meier‐Augenstein, W., Smith, K., Bohé, J., Wolfe, R. R., Gibson, J. N. A., Adams, C. E., & Rennie, M. J. (2002) · Biochemical Society Transactions · doi:10.1042/bst0300061 · Cited by 52 · see citing works
Can oestrogen influence skeletal muscle damage, inflammation, and repair?
Tiidus, P. M. (2005) · British Journal of Sports Medicine · doi:10.1136/bjsm.2005.016881 · Cited by 51 · see citing works
Testosterone Prohormone Supplements
Brown, G. A., Vukovich, M. D., & King, D. S. (2006) · Medicine & Science in Sports & Exercise · doi:10.1249/01.mss.0000228928.69512.2e · Cited by 46 · see citing works
Muscular Transcriptome in Postmenopausal Women With or Without Hormone Replacement
Pöllänen, E., Ronkainen, P. H. A., Suominen, H., Takala, T., Koskinen, S. O. A., Puolakka, J. O., Sipilä, S., & Kovanen, V. (2007) · Rejuvenation Research · doi:10.1089/rej.2007.0536 · Cited by 41 · see citing works
Oestrogen-progestogen replacement therapy changes body composition in early post-menopausal women
Jensen, J., Christiansen, C., & Rødbro, P. (1986) · Maturitas · doi:10.1016/0378-5122(86)90028-9 · Cited by 39 · see citing works
Oral contraceptive use by teenage women does not affect body composition
Lloyd, T. (2002) · Obstetrics and Gynecology · doi:10.1016/s0029-7844(02)02056-2 · Cited by 35 · see citing works
Wreje, U., Brynhildsen, J., Åberg, H., Byström, B., Hammar, M. L., & von Schoultz, B. (2000) · Contraception · doi:10.1016/s0010-7824(00)00106-2 · Cited by 32 · see citing works
Ovariectomy, hindlimb unweighting, and recovery effects on skeletal muscle in adult rats.
Brown, M., Foley, A. M., & Ferrería, J. (2005) · PubMed · Cited by 30 · see citing works
Rennie, M. J., Phillips, S. M., & Smith, K. (2008) · Journal of Applied Physiology · doi:10.1152/japplphysiol.90782.2008 · Cited by 23 · see citing works
Hansen, M., Trappe, T. A., Crameri, R. M., Qvortrup, K., Kjær, M., & Langberg, H. (2009) · Scandinavian Journal of Medicine and Science in Sports · doi:10.1111/j.1600-0838.2007.00766.x · Cited by 21 · see citing works
Self-citationMenstruation in Girls and Adolescents
Committee on Adolescence (2014) · American Academy of Pediatrics eBooks · doi:10.1542/9781581108613-part05-menstruation · Cited by 5 · see citing works
Changes in medication use from age 26 to 32 in a representative birth cohort
Thomson, W. M., Poulton, R., Hancox, R. J., et al. (2007) · Internal Medicine Journal · doi:10.1111/j.1445-5994.2007.01360.x · Cited by 4 · see citing works
[Oral contraceptives. General aspects].
Lidegaard, Ø., Schiødt, A. V., & Ef, P. (2001) · PubMed · Cited by 3 · see citing works
Oral Contraceptive Use by Teenage Women Does Not Affect Body Composition
Lloyd, T., Lin, H. M., Matthews, A. E., et al. (2002) · Obstetrics and Gynecology · doi:10.1097/00006250-200208000-00006 · Cited by 3 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Effect of Estrogen on Musculoskeletal Performance and Injury Risk
Chidi-Ogbolu, N., & Baar, K. (2019) · Frontiers in Physiology · doi:10.3389/fphys.2018.01834 · Cited by 389 · see citing works
Aging of the musculoskeletal system: How the loss of estrogen impacts muscle strength
Collins, B. C., Laakkonen, E. K., & Lowe, D. A. (2019) · Bone · doi:10.1016/j.bone.2019.03.033 · Cited by 227 · see citing works
Tanner, R. E., Brunker, L. B., Agergaard, J., Barrows, K. M., Briggs, R. A., Kwon, O. S., Young, L. M., Hopkins, P. N., Volpi, E., Marcus, R. L., et al. (2015) · The Journal of Physiology · doi:10.1113/jp270699 · Cited by 223 · see citing works
Smith, G. I., Yoshino, J., Reeds, D. N., Bradley, D., Burrows, R. E., Heisey, H. D., Moseley, A. C., & Mittendorfer, B. (2014) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2013-2835 · Cited by 124 · see citing works
International society of sports nutrition position stand: nutritional concerns of the female athlete
Sims, S. T., Kerksick, C. M., Smith-Ryan, A. E., Janse de Jonge, X. A. K., Hirsch, K. R., Arent, S. M., Hewlings, S. J., Kleiner, S. M., Bustillo, E., Tartar, J. L., et al. (2023) · Journal of the International Society of Sports Nutrition · doi:10.1080/15502783.2023.2204066 · Cited by 91 · see citing works
Pinckaers, P. J., Kouw, I. W., Gorissen, S. H., Houben, L. H., Senden, J. M., Wodzig, W. K., de Groot, L. C., Verdijk, L. B., Snijders, T., & van Loon, L. J. (2022) · The Journal of Nutrition · doi:10.1093/jn/nxac222 · Cited by 70 · see citing works
Influence of Oral Contraceptive Use on Adaptations to Resistance Training
Dalgaard, L. B., Dalgas, U., Andersen, J. L., Rossen, N. B., Møller, A. B., Stødkilde-Jørgensen, H., Jørgensen, J. O., Kovanen, V., Couppé, C., Langberg, H., et al. (2019) · Frontiers in Physiology · doi:10.3389/fphys.2019.00824 · Cited by 58 · see citing works
Self-citationProtein Requirements of Pre-Menopausal Female Athletes: Systematic Literature Review
Mercer, D., Convit, L., Condo, D., Carr, A. J., Hamilton, D. L., Slater, G., & Snipe, R. M. J. (2020) · Nutrients · doi:10.3390/nu12113527 · Cited by 20 · see citing works
- Authors
- Hansen, M., Langberg, H., Holm, L., Miller, B. F., Petersen, S. G., Doessing, S., Skovgaard, D., Trappe, T., & Kjaer, M.
- Affiliations
- University of Copenhagen (DK)
- Colorado State University (US)
- Ball State University (US)
- Corresponding author
- Hansen, M.
- Venue
- Scandinavian Journal of Medicine & Science in Sports
- Publisher
- Wiley
- Published
- 2011-01-11
- Volume
- 21
- Issue
- 1
- Pages
- 62-72
- ISSN
- 0905-7188 (ISSN-L)
- 1600-0838
- OpenAlex
- W1552988067
- Cited by
- 57
- References
- 44
- Citations by year
- 2026: 5 · 2025: 6 · 2024: 7 · 2023: 5 · 2022: 3 · 2021: 2 · 2020: 4 · 2019: 5 · 2018: 1 · 2017: 2 · 2016: 4 · 2015: 4 · 2014: 2 · 2013: 4 · 2012: 2
- FWCI
- 0.44
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Scandinavian Journal of Medicine and Science in Sports (publishedVersion)
- PubMed (publishedVersion)
- Research at the University of Copenhagen (University of Copenhagen) (submittedVersion)
- University of Birmingham Research Portal (University of Birmingham) (submittedVersion)
- Funders
- Aarhus Universitetshospital
- Gigtforeningen
- Eva og Henry Frænkels Mindefond
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Muscle metabolism and nutrition
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Health Sciences › Medicine › Physiology › Nutrition and Health in Aging
- Keywords
- oral contraceptives, myofibrillar protein synthesis, protein turnover, protein degradation, 3-methylhistidine, fractional synthesis rate, skeletal muscle, androstenedione, young women, physical activity
- MeSH terms
- Analysis of Variance, Energy Intake, Contraceptives, Oral, Exercise Test, Female, Humans, Leg, Methylhistidines, Muscle Proteins, Myofibrils, Proline, Muscle, Skeletal, Young Adult
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
