ORIGINAL ARTICLE: Endometrial Osteopontin mRNA Expression and Plasma Osteopontin Levels are Increased in Patients with Endometriosis
Open accessAbstract
Problem The aim of this study was to evaluate osteopontin (OPN) mRNA expression in eutopic endometrium and plasma OPN levels in patients with endometriosis. Method of study A total of 79 patients with histologically confirmed endometriosis and 43 patients without endometriosis participated in this study. OPN mRNA expression in endometrial tissues was measured by real‐time quantitative polymerase chain reaction (PCR) and plasma concentrations of OPN were quantified using a specific commercial sandwich enzyme‐linked immunosorbent assays (ELISA). Results Osteopontin mRNA expression in endometrial tissue was significantly higher in women with endometriosis than in controls ( P = 0.010). The mean plasma levels of OPN (mean ± S.E.M.) in patients with endometriosis and controls were 407.31 ± 37.80 ng/mL and 165.84 ± 19.29 ng/mL, respectively ( P < 0.001). Receiver operating characteristic (ROC) analysis for plasma OPN revealed an area under the curve (AUC) of 0.894, with a sensitivity of 93.0%, specificity of 72.4%, positive likelihood ratio of 3.37, and negative likelihood ratio of 0.1 using a cut‐off value of 167.68 ng/mL. Conclusion Osteopontin may be involved in the pathogenesis of endometriosis and plasma OPN may be a useful non‐invasive marker for the diagnosis of endometriosis.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Osteopontin was elevated in plasma for all disease stages.
“In addition, the cell adhesion molecule osteopontin was elevated in plasma for all disease stages [60, 61].”
From Update on biomarkers for the detection of endometriosis (2015) · cites this paper as [61]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method
Livak, K. J., & Schmittgen, T. D. (2001) · Methods · doi:10.1006/meth.2001.1262 · Cited by 180,860 · see citing works
The meaning and use of the area under a receiver operating characteristic (ROC) curve.
Hanley, J. A., & McNeil, B. J. (1982) · Radiology · doi:10.1148/radiology.143.1.7063747 · Cited by 21,856 · see citing works
Revised American Society for Reproductive Medicine classification of endometriosis: 1996
American Society for Reproductive Medicine (1997) · Fertility and Sterility · doi:10.1016/s0015-0282(97)81391-x · Cited by 3,018 · see citing works
Sampson, J. A. (1927) · American Journal of Obstetrics and Gynecology · doi:10.1016/s0002-9378(15)30003-x · Cited by 2,334 · see citing works
Osteopontin--a possible anchor of osteoclasts to bone.
Reinholt, F. P., Hultenby, K. R., Oldberg, Å., & Heinegård, D. K. (1990) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.87.12.4473 · Cited by 827 · see citing works
Scatena, M., Liaw, L., & Giachelli, C. M. (2007) · Arteriosclerosis Thrombosis and Vascular Biology · doi:10.1161/atvbaha.107.144824 · Cited by 644 · see citing works
The Epidemiology of Endometriosis
CRAMER, D. W., & MISSMER, S. A. (2002) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.2002.tb02761.x · Cited by 539 · see citing works
Liaw, L., Skinner, M. P., Raines, E. W., Ross, R., Cheresh, D. A., Schwartz, S. M., & Giachelli, C. M. (1995) · Journal of Clinical Investigation · doi:10.1172/jci117718 · Cited by 511 · see citing works
Osteopontin as a Potential Diagnostic Biomarker for Ovarian Cancer
Kim, J. (2002) · JAMA · doi:10.1001/jama.287.13.1671 · Cited by 489 · see citing works
Self-citationBrown, L. F., Berse, B., Water, L. V. D., Papadopoulos-Sergiou, A., Perruzzi, C. A., Manseau, E. J., Dvorak, H. F., & Senger, D. R. (1992) · Molecular Biology of the Cell · doi:10.1091/mbc.3.10.1169 · Cited by 466 · see citing works
Elevated serum bone sialoprotein and osteopontin in colon, breast, prostate, and lung cancer.
Fedarko, N. S., Jain, A., Karadağ, A., Eman, M. R. V., & Fisher, L. W. (2001) · PubMed · Cited by 380 · see citing works
The performance of CA-125 measurement in the detection of endometriosis: a meta-analysis
Mol, B. W. J., Bayram, N., Lijmer, J. G., Wiegerinck, M. A. H. M., Bongers, M. Y., van der Veen, F., & Bossuyt, P. M. M. (1998) · Fertility and Sterility · doi:10.1016/s0015-0282(98)00355-0 · Cited by 355 · see citing works
A Biochemical Characterization of the Binding of Osteopontin to Integrins αvβ1 and αvβ5
Hu, D. D., Lin, E. C. K., Kovach, N. L., Hoyer, J. R., & Smith, J. W. (1995) · Journal of Biological Chemistry · doi:10.1074/jbc.270.44.26232 · Cited by 255 · see citing works
The Role of Osteopontin in Tumor Progression and Metastasis in Breast Cancer
Rodrigues, L. R., Teixeira, J. A., Schmitt, F. C., Paulsson, M. A., & Lindmark-Månsson, H. (2007) · Cancer Epidemiology Biomarkers & Prevention · doi:10.1158/1055-9965.epi-06-1008 · Cited by 242 · see citing works
Berkkanoğlu, M., & Arici, A. M. (2003) · American Journal of Reproductive Immunology · doi:10.1034/j.1600-0897.2003.00042.x · Cited by 240 · see citing works
Advances in the management of endometriosis: an update for clinicians
Crosignani, P., Olive, D., Bergqvist, A., & Luciano, A. (2006) · Human Reproduction Update · doi:10.1093/humupd/dmi049 · Cited by 237 · see citing works
Apparao, K. B. C., Murray, M. J., Fritz, M. A., Meyer, W. R., Chambers, A. F., Truong, P., & Lessey, B. A. (2001) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem.86.10.7906 · Cited by 198 · see citing works
Plasma osteopontin levels are associated with the presence and extent of coronary artery disease
Ohmori, R., Momiyama, Y., Taniguchi, H., Takahashi, R., Kusuhara, M., Nakamura, H., & Ohsuzu, F. (2003) · Atherosclerosis · doi:10.1016/s0021-9150(03)00298-3 · Cited by 185 · see citing works
The Role of Endometrium in Endometriosis
Ulukuş, M., Çakmak, H., & Arici, A. M. (2006) · Journal of the Society for Gynecologic Investigation · doi:10.1016/j.jsgi.2006.07.005 · Cited by 171 · see citing works
Crawford, H. C., Matrisian, L. M., & Liaw, L. (1998) · PubMed · Cited by 165 · see citing works
Spaczyński, R., & Dulęba, A. J. (2003) · Seminars in Reproductive Medicine · doi:10.1055/s-2003-41326 · Cited by 158 · see citing works
Reduced expression of biomarkers associated with the implantation window in women with endometriosis
Wei, Q., Clair, J. B. S., Fu, T., Stratton, P., & Nieman, L. K. (2008) · Fertility and Sterility · doi:10.1016/j.fertnstert.2008.02.121 · Cited by 133 · see citing works
CA 125 in Benign Gynecological Conditions
Meden, H., & Fattahi-Meibodi, A. (1998) · The International Journal of Biological Markers · doi:10.1177/172460089801300411 · Cited by 126 · see citing works
Wong, C., Lit, L. C., Tam, L.-S., Li, E., & Lam, C.-W.-K. (2005) · Rheumatology · doi:10.1093/rheumatology/keh558 · Cited by 115 · see citing works
Implantation Defects in Infertile Women with Endometriosis
Lessey, B. A. (2002) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.2002.tb02787.x · Cited by 92 · see citing works
Agnholt, J. S., Kelsen, J., Schack, L., Hvas, C. L., Dahlerup, J. F., & Sørensen, E. S. (2007) · Scandinavian Journal of Immunology · doi:10.1111/j.1365-3083.2007.01908.x · Cited by 74 · see citing works
Apparao, K. B. C. (2001) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.86.10.4991 · Cited by 65 · see citing works
Bautista, D. S., Saad, Z., Chambers, A. F., et al. (1996) · Clinical Biochemistry · doi:10.1016/0009-9120(96)84728-a · Cited by 63 · see citing works
Clinical significance of osteopontin expression in cervical cancer
Cho, H., Hong, S. W., Oh, Y. J., et al. (2008) · Journal of Cancer Research and Clinical Oncology · doi:10.1007/s00432-007-0351-5 · Cited by 54 · see citing works
Gene Expression Profiling of the Rat Endometriosis Model
Konno, R., Fujiwara, H., Netsu, S., Odagiri, K., Shimane, M., Nomura, H., & Suzuki, M. (2007) · American Journal of Reproductive Immunology · doi:10.1111/j.1600-0897.2007.00507.x · Cited by 52 · see citing works
Future Directions in Endometriosis Research
D’Hooghe, T. M., Kyama, C. M., Debrock, S., Meuleman, C., & Mwenda, J. M. (2004) · Annals of the New York Academy of Sciences · doi:10.1196/annals.1335.034 · Cited by 36 · see citing works
Odagiri, K., Konno, R., Fujiwara, H., Netsu, S., Ohwada, M., Shibahara, H., & Suzuki, M. (2007) · Fertility and Sterility · doi:10.1016/j.fertnstert.2007.02.007 · Cited by 16 · see citing works
Flagged first, then by citations
Research works printed but not linked: 1
- Scatena M, Liaw L, Giachelli CM: Osteopontin: a multifunctional molecule regulating chronic inflammation and vascular disease. Arterioscler Thromb Vasc Biol 2007; 27:2302-2309.
Cited by
Sorted by citations
Peripheral biomarkers of endometriosis: a systematic review
May, K. E., Conduit-Hulbert, S. A., Villar, J., Kirtley, S., Kennedy, S. H., & Becker, C. M. (2010) · Human Reproduction Update · doi:10.1093/humupd/dmq009 · Cited by 418 · see citing works
Zhang, Q., Duan, J., Liu, X., & Guo, S.-W. (2016) · Molecular and Cellular Endocrinology · doi:10.1016/j.mce.2016.03.015 · Cited by 211 · see citing works
Update on biomarkers for the detection of endometriosis
Fassbender, A., Burney, R. O., F. O, D., D’Hooghe, T., & Giudice, L. (2015) · BioMed Research International · doi:10.1155/2015/130854 · Cited by 195 · see citing works
Evaluation of a panel of 28 biomarkers for the non-invasive diagnosis of endometriosis
Vodolazkaia, A., El-Aalamat, Y., Popovic, D., Mihalyi, A., Bossuyt, X., Kyama, C. M., Fassbender, A., Bokor, A., Schols, D., Huskens, D., et al. (2012) · Human Reproduction · doi:10.1093/humrep/des234 · Cited by 189 · see citing works
Hypoxia: The force of endometriosis
Wu, M., Hsiao, K., & Tsai, S. (2019) · Journal of Obstetrics and Gynaecology Research · doi:10.1111/jog.13900 · Cited by 88 · see citing works
An integrated multi-tissue approach for endometriosis candidate biomarkers: a systematic review
Brulport, A., Bourdon, M., Vaiman, D., Drouet, C., Pocate-Cheriet, K., Bouzid, K., Marcellin, L., Santulli, P., Abo, C., Jeljeli, M., et al. (2024) · Reproductive Biology and Endocrinology · doi:10.1186/s12958-023-01181-8 · Cited by 52 · see citing works
Pathological functions of hypoxia in endometriosis
Hsiao, K.-Y. (2015) · Frontiers in Bioscience · doi:10.2741/e736 · Cited by 61 · see citing works
Expression and localisation of osteopontin and prominin-1 (CD133) in patients with endometriosis
D’AMICO, F., SKARMOUTSOU, E., QUADERNO, G., MALAPONTE, G., LA CORTE, C., SCIBILIA, G., D’AGATE, G., SCOLLO, P., FRAGGETTA, F., SPANDIDOS, D. A., et al. (2013) · International Journal of Molecular Medicine · doi:10.3892/ijmm.2013.1325 · Cited by 28 · see citing works
Biomarker development in endometriosis
Burney, R. O. (2014) · Scandinavian Journal of Clinical and Laboratory Investigation · doi:10.3109/00365513.2014.936692 · Cited by 16 · see citing works
- Authors
- Cho, S., Ahn, Y. S., Choi, Y. S., Seo, S. K., Nam, A., Kim, H. Y., Kim, J., Park, K. H., Cho, D. J., & Lee, B. S.
- Affiliations
- Yonsei University (KR)
- Corresponding author
- Seo, Seok Kyo
- Lee, Byung Seok
- Venue
- American Journal of Reproductive Immunology
- Publisher
- Wiley
- Published
- 2009-03-02
- Volume
- 61
- Issue
- 4
- Pages
- 286-293
- ISSN
- 0271-7352 (ISSN-L)
- 1046-7408
- 1600-0897
- OpenAlex
- W1509571353
- Cited by
- 51
- References
- 32
- Citations by year
- 2026: 1 · 2024: 3 · 2023: 3 · 2022: 2 · 2020: 1 · 2019: 7 · 2018: 3 · 2017: 7 · 2016: 5 · 2015: 3 · 2014: 1 · 2013: 5 · 2012: 4
- FWCI
- 3.94
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- American Journal of Reproductive Immunology (publishedVersion)
- PubMed (publishedVersion)
- YUHSpace (Yonsei University Medical Library) (submittedVersion, cc-by-nc-nd)
- Topics
- Health Sciences › Medicine › Reproductive Medicine › Endometriosis Research and Treatment
- Health Sciences › Medicine › Rheumatology › Bone and Dental Protein Studies
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Ectopic Pregnancy Diagnosis and Management
- Keywords
- osteopontin, endometriosis, biomarkers, non-invasive diagnosis, endometrium, endometriosis pathogenesis
- MeSH terms
- Adolescent, Adult, Endometriosis, Endometrium, Enzyme-Linked Immunosorbent Assay, Female, Humans, Middle Aged, RNA, Messenger, ROC Curve, Biomarkers, CA-125 Antigen, Gene Expression Profiling, Osteopontin
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
