Effects of Overexpression of the Endogenous Farnesyl Diphosphate Synthase on the Artemisinin Content in Artemisia annua L.
Abstract
Artemisinin is a novel effective antimalarial drug extracted from the medicinal plant Artemisia annua L. Owing to the tight market and low yield of artemisinin, there is great interest in enhancing the production of artemisinin. In the present study, farnesyl diphosphate synthase (FPS) was overexpressed in high‐yield A. annua to increase the artemisinin content. The FPS activity in transgenic A. annua was two‐ to threefold greater than that in non‐transgenic A. annua. The highest artemisinin content in transgenic A. annua was approximately 0.9% (dry weight), which was 34.4% higher than that in non‐transgenic A. annua. The results demonstrate the regulatory role of FPS in artemisinin biosynthesis. (Managing editor: Wei Wang)
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Upregulation of FDS by genetic transformation of A. annua using a constitutive promoter resulted in increased artemisinin production.
“upregulation of FDS by genetic transformation of A. annua using constitutive promoter resulted in an increased artemisinin production [29]”
From Relative expression of genes of terpene metabolism in different tissues of Artemisia annuaL (2011) · cites this paper as [29]
FDS1 may be a rate-limiting enzyme in artemisinin biosynthesis.
“indicating that FDS1 also may be a rate-limiting enzyme in artemisinin biosynthesis”
From Relative expression of genes of terpene metabolism in different tissues of Artemisia annuaL (2011) · cites this paper as [29]
Overexpression of FDS1 in A. annua results in an increase in artemisinin production, indicating it is a rate-limiting enzyme in this pathway.
“Overexpression of FDS1 in A. annua results in an increase in artemisinin production [29,30] indicating that it is a rate-limiting enzyme in this pathway.”
From Relative expression of genes of terpene metabolism in different tissues of Artemisia annuaL (2011) · cites this paper as [29]
Investigators have concentrated on expressing enzymes from phases 1 and 2 of the artemisinin biosynthetic route in transgenic organisms, including HMGR, FPPS, ADS, and CYP71AV1.
“To date, investigators have concentrated on expressing the enzymes from phases 1 and 2 of the biosynthetic route to artemisinin in transgenic organisms (see Scheme 9 in Section 3). These have included: HMG-CoA reductase (HMGR) [370]; farnesyl diphosphate synthase (FPPS) [371, 372,373,374]; amorpha-4,11-diene synthase (ADS) [375,376,377]; and the P450 enzyme which oxidises amorpha-4,11-diene to art”
From The Biosynthesis of Artemisinin (Qinghaosu) and the Phytochemistry of Artemisia annua L. (Qinghao) (2010) · cites this paper as [373]
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- Authors
- Han, J., Liu, B., Ye, H., Wang, H., Li, Z., & Li, G.
- Affiliations
- Chinese Academy of Sciences (CN)
- ORCID
- Wang, Hong
- Venue
- Journal of Integrative Plant Biology
- Publisher
- Wiley
- Published
- 2006-04
- Volume
- 48
- Issue
- 4
- Pages
- 482-487
- ISSN
- 0577-7496 (ISSN-L)
- 1672-9072
- 1744-7909
- OpenAlex
- W1995977555
- Cited by
- 115
- References
- 18
- Citations by year
- 2026: 3 · 2025: 4 · 2024: 5 · 2023: 4 · 2022: 4 · 2021: 3 · 2020: 3 · 2018: 7 · 2017: 7 · 2016: 8 · 2015: 7 · 2014: 7 · 2013: 10 · 2012: 9
- FWCI
- 2.31
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Integrative Plant Biology (publishedVersion)
- Institute of Botany, CAS Institutional Repository (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Sesquiterpenes and Asteraceae Studies
- Keywords
- artemisinin, Artemisia annua, farnesyl diphosphate synthase, overexpression, transgenic plants, artemisinin biosynthesis, terpene synthase, metabolic engineering
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