Over-expression of HMG-CoA reductase and amorpha-4,11-diene synthase genes in Artemisia annua L. and its influence on artemisinin content

Alam, Pravej; Abdin, M. Z.

doi:10.1007/s00299-011-1099-6

Cited by 108Plant biochemistry and biosynthesisPlant tissue culture and regenerationTransgenic Plants and Applications

Over-expression of HMG-CoA reductase and amorpha-4,11-diene synthase genes in Artemisia annua L. and its influence on artemisinin content

Alam, Pravej, Abdin, M. Z.·2011·Plant Cell Reports

DOI 10.1007/s00299-011-1099-6

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Abstract

Artemisinin, an endoperoxide sesquiterpene lactone, is a novel antimalarial natural product isolated from Artemisia annua L. plants. The low concentrations (0.01-1.1%) of this compound in A. annua L. plants is, however, a major constraint for commercialization of artemisinin-based combination therapies (ACTs) recommended by WHO for treating malaria caused by multidrug-resistant P. falciparum sp. In this context, in vivo yield improvement programs were undertaken by us. In the present study, HMG-Co A reductase gene (hmgr) from Catharanthus roseus (L) G. Don and amorpha-4,11-diene synthase (ads) gene from A. annua L. were over-expressed in A. annua L. plants to study their effects on artemisinin yields. The transgenic lines developed from putative transgenic regenerants were evaluated for integration and copy number of the transgenes using hptII gene probe, as it was a part of the expression cassette. The transgenic lines showed positive bands of hptII gene on Southern blots confirming the integration of transgenes. Some of the transgenic lines had single copy of the transgenes, while others had multiple copies. The expressions of hmgr and ads at the transcriptional level were also confirmed in each transgenic line employing RT-PCR assays. The HPLC analyses showed that the artemisinin contents were significantly increased in these transgenics. One of the transgenic lines, TR4, was found to contain 7.65-fold higher (1.73 mg/gDW) artemisinin than the non-transgenic plant (W). The increased artemisinin levels were found to be correlated with HMG-Co A reductase and amorpha-4,11-diene synthase enzymatic activities in the biochemical analyses.

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Artemisinin biosynthesis was improved 7.65-fold through co-transformation of both ADS and HMGR in A. annua.
“Artemisinin biosynthesis was improved 7.65fold through co-transformation of both ADS and HMGR in A. annua (Alam and Abdin 2011)”
From Enhancing artemisinin content in and delivery from Artemisia annua: a review of alternative, classical, and transgenic approaches (2021) · cites this paper as Alam and Abdin 2011

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Authors: Alam, P., & Abdin, M. Z.
Affiliations: Jamia Hamdard (IN)
Corresponding author: Abdin, M. Z.
ORCID: Alam, Pravej
Abdin, M. Z.
Venue: Plant Cell Reports
Publisher: Springer Science and Business Media LLC
Published: 2011-06-08
Volume: 30
Issue: 10
Pages: 1919-1928
ISSN: 0721-7714 (ISSN-L)
1432-203X
DOI: 10.1007/s00299-011-1099-6
OpenAlex: W2152038789
Cited by: 108
References: 51
Citations by year: 2026: 3 · 2025: 8 · 2024: 7 · 2023: 5 · 2022: 8 · 2021: 4 · 2020: 6 · 2019: 5 · 2018: 7 · 2017: 11 · 2016: 10 · 2015: 11 · 2014: 8 · 2013: 7 · 2012: 6
FWCI: 2.99
Type: journal article
Language: en
Open access: closed
Licence: www.springer.com/tdm (tdm)
Locations: Plant Cell Reports (publishedVersion)
PubMed (publishedVersion)
Funders: Department of Science and Technology, Ministry of Science and Technology, India
Institute of Psychology, Chinese Academy of Sciences
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Transgenic Plants and Applications
Keywords: artemisinin, Artemisia annua, HMG-CoA reductase, amorpha-4,11-diene synthase, transgenic plants, gene overexpression, sesquiterpene lactone biosynthesis, transgene integration, transgene copy number, Catharanthus roseus
MeSH terms: Genetic Engineering, Hydroxymethylglutaryl CoA Reductases, Plant Proteins, Transformation, Genetic, Gene Expression Regulation, Plant, Gene Dosage, Transgenes, Alkyl and Aryl Transferases, Plants, Genetically Modified, Artemisia annua, Artemisinins

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Over-expression of HMG-CoA reductase and amorpha-4,11-diene synthase genes in Artemisia annua L. and its influence on artemisinin content

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